Click here to switch to the map view.

The map label for this gene is rrmJ [H]

Identifier: 15602302

GI number: 15602302

Start: 512402

End: 513031

Strand: Direct

Name: rrmJ [H]

Synonym: PM0437

Alternate gene names: 15602302

Gene position: 512402-513031 (Clockwise)

Preceding gene: 15602295

Following gene: 15602303

Centisome position: 22.7

GC content: 43.97

Gene sequence:

>630_bases
ATGGGAAAGAAAAAACGTTCAGCGAGTTCTTCTCGCTGGCTAAATGAGCATTTTAAAGATCCGTTTGTCCAGAAAGCACA
TAAACAAAAATTGCGTTCACGAGCCTATTTTAAGTTAGATGAGATCCAACAAACTGACCGTTTATTCAAGCCCGGTATGA
CAGTGGTCGATTTAGGTGCGGCACCGGGTGGCTGGTCCCAGTATGTCGTGAGCCAAATAGGTGGTGCGGGACGCATTATC
GCCTGTGATATTTTGGAAATGGATCCGATTGTAGGCGTCGATTTTTTACAAGGCGATTTTCGTGATGAAAAGGTGTTAGC
TGCGTTATTAGCGCGTGTTGGTGAAGGTAAAGTGGACGTGGTGATGTCAGATATGGCGCCTAATTTTAGTGGTATGCCAT
CGGTAGATATTCCGCGTGCGATGTATTTAGTTGAGCTGGCACTGGATATGTGTAAACAAGTGCTTGCGACAAAAGGCAGT
TTTGTCGTAAAAGTTTTTCAGGGTGAAGGTTTTGATGAATACTTGAGAGAAATTCGTTCTCTCTTTAGTGTTGTAAAAGT
GCGGAAACCTGAGGCTTCTCGAGGTCGTTCTCGTGAGGTGTATATTGTTGCAACCGGTTATAAAGGATAG

Upstream 100 bases:

>100_bases
TAGCCAATATTGATTCAATTCATTTTTTCTACCAAATTCGCCTTGAAACTTGTGTATAATGCCCCATATTGAAAAAACAT
CGAAGCAATAGAAGAAGTTT

Downstream 100 bases:

>100_bases
GCTTACTTACGTTGAGTAAGTTTATTCGTTAATGACTATTGATGGGATAATTTCCAATTTCATTACAGATATAGTAATCT
TTAGCAACAAATTATTAACG

Product: 23S rRNA methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MGKKKRSASSSRWLNEHFKDPFVQKAHKQKLRSRAYFKLDEIQQTDRLFKPGMTVVDLGAAPGGWSQYVVSQIGGAGRII
ACDILEMDPIVGVDFLQGDFRDEKVLAALLARVGEGKVDVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCKQVLATKGS
FVVKVFQGEGFDEYLREIRSLFSVVKVRKPEASRGRSREVYIVATGYKG

Sequences:

>Translated_209_residues
MGKKKRSASSSRWLNEHFKDPFVQKAHKQKLRSRAYFKLDEIQQTDRLFKPGMTVVDLGAAPGGWSQYVVSQIGGAGRII
ACDILEMDPIVGVDFLQGDFRDEKVLAALLARVGEGKVDVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCKQVLATKGS
FVVKVFQGEGFDEYLREIRSLFSVVKVRKPEASRGRSREVYIVATGYKG
>Mature_208_residues
GKKKRSASSSRWLNEHFKDPFVQKAHKQKLRSRAYFKLDEIQQTDRLFKPGMTVVDLGAAPGGWSQYVVSQIGGAGRIIA
CDILEMDPIVGVDFLQGDFRDEKVLAALLARVGEGKVDVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCKQVLATKGSF
VVKVFQGEGFDEYLREIRSLFSVVKVRKPEASRGRSREVYIVATGYKG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=215, Percent_Identity=34.8837209302326, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI194097365, Length=207, Percent_Identity=32.8502415458937, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI29029591, Length=191, Percent_Identity=32.9842931937173, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI29029589, Length=191, Percent_Identity=32.9842931937173, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI7110661, Length=191, Percent_Identity=32.9842931937173, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=81.7307692307692, Blast_Score=349, Evalue=7e-98,
Organism=Caenorhabditis elegans, GI17553474, Length=217, Percent_Identity=33.6405529953917, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71987550, Length=208, Percent_Identity=31.7307692307692, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17554650, Length=192, Percent_Identity=30.2083333333333, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71987561, Length=208, Percent_Identity=31.7307692307692, Blast_Score=93, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17553860, Length=190, Percent_Identity=32.1052631578947, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6319535, Length=200, Percent_Identity=35, Blast_Score=109, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319796, Length=210, Percent_Identity=29.5238095238095, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6321302, Length=96, Percent_Identity=42.7083333333333, Blast_Score=79, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356387, Length=215, Percent_Identity=33.4883720930233, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18859957, Length=207, Percent_Identity=33.8164251207729, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648639, Length=194, Percent_Identity=33.5051546391753, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24647580, Length=199, Percent_Identity=29.6482412060301, Blast_Score=91, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR004512
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 23271; Mature: 23140

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKKRSASSSRWLNEHFKDPFVQKAHKQKLRSRAYFKLDEIQQTDRLFKPGMTVVDLGA
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
APGGWSQYVVSQIGGAGRIIACDILEMDPIVGVDFLQGDFRDEKVLAALLARVGEGKVDV
CCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCEEE
VMSDMAPNFSGMPSVDIPRAMYLVELALDMCKQVLATKGSFVVKVFQGEGFDEYLREIRS
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHH
LFSVVKVRKPEASRGRSREVYIVATGYKG
HHHHHHHCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure 
GKKKRSASSSRWLNEHFKDPFVQKAHKQKLRSRAYFKLDEIQQTDRLFKPGMTVVDLGA
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
APGGWSQYVVSQIGGAGRIIACDILEMDPIVGVDFLQGDFRDEKVLAALLARVGEGKVDV
CCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCEEE
VMSDMAPNFSGMPSVDIPRAMYLVELALDMCKQVLATKGSFVVKVFQGEGFDEYLREIRS
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHH
LFSVVKVRKPEASRGRSREVYIVATGYKG
HHHHHHHCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA