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The map label for this gene is metE

Identifier: 15602285

GI number: 15602285

Start: 492952

End: 495225

Strand: Reverse

Name: metE

Synonym: PM0420

Alternate gene names: 15602285

Gene position: 495225-492952 (Counterclockwise)

Preceding gene: 15602290

Following gene: 15602284

Centisome position: 21.94

GC content: 43.71

Gene sequence:

>2274_bases
ATGACAACATTTCATGTAGCAGGCTTTCCGCGTGTGGGTGCAAAACGTGAACTAAAATTTGCCCAAGAGCGTTATTGGCG
TGGCGAAATTGCCGAACAGGATCTGTTAGAGATTGCACAAAAATTACGTGAAATTAACTGGAAACACCAAGCAGCAGCCA
ATGCGGACTTCGTGGCAGTCGCCGATTTCACATTCTACGATCATATTTTGGATCTGCAAGTAGCAACAGGCGCTATTCCA
GCCCGTTTCGGTTTCGATAGCCAAAACTTAAGTTTAAATGAATATTTCCAACTTGCACGCGGTAACCAAACCCAATTTGC
CATTGAAATGACCAAATGGTTTGATACAAACTACCACTATTTAGTACCAGAATTCACTAAAAATACCGAATTTAAAGCGA
ACCCAGCACATTACGTACAACAAATCCGTGAAGCGAAAGCCTTAGGTTTCAAATTTAAACCAACAATCGTGGGACCATTA
ACGTTCCTTTGGTTAGGTAAAGAAAAAGGCGAGGCCTTTAATCGTTTTGAATTACTGGCAAAACTCGTACCTGTGTATGT
AGAAATCCTCAATGCCTTAGTAGCAGAAGGCGCAGAGTGGATCCAAATTGATGAACCGGCTTTAGCGGTGGATCTACCAA
CAGAATGGATTGAAGCTTACAAAGCTGTTTATACGACATTGAAAGAAAAAGTAAAAGCAAAACTCTTACTAGCGACTTAT
TTCGGTTCTGTTGCAGAACACGCGCCACTGCTTAAAGGTTTACCAGTCGATGGTTTACACATTGATTTAGTGCGTGCACC
TGCACAGCTTGCTGCCTTTGAAGATTACAACAAAGTGCTTTCTGTTGGTGTCATTGACGGTCGTAACATTTGGCGTGCCA
ACCTCAACCAAGTACTCGATGTAGTTGAACCATTGAAAGCAAAATTCGGTGAAAATCTATGGATTGCGCCAAGTTGTTCA
CTCCTCCACACACCATATGATTTAGAAGTGGAAACGCAATTAAAAGCGAATAAACCGGAGCTATACAGCTGGTTAGCCTT
CACGTTACAAAAAGTGCAAGAATTGCGTGTCATTAAGACCGCACTTGAGCAAGGTCGTGGCGCAGTACAAGCTGAACTTG
ATGCAAGCCAAGCAGCTGCTGACGCCCGTGCAAACAGCAAAGAAATTCATCGTCCAGAAGTGGCAGAACGTTTAGCAAAC
TTGCCGACGGATGCGGACAAACGTAAATCGCCTTTTGCAGAACGTATTGCAAAACAAAATGCGTGGTTAAATTTACCGTT
ATTACCAACCACGAATATCGGTTCCTTCCCACAAACCGTTGAAATTCGCCAAGCCCGTGCGAAATTCAAGAAAGGCGAAC
TTAGCGTCGCAGATTATGAAGCGGCAATGAAGAAAGAAATCGAATTTGTGGTACGTCGCCAAGAAGAACTGGATTTAGAT
GTGTTAGTGCACGGTGAAGCAGAGCGTAACGACATGGTGGAATACTTCGGTGAGTTACTCGACGGTTTCGCTTTCACAAA
ATTCGGTTGGGTACAAAGCTACGGTTCACGTTGCGTGAAGCCACCAGTGATTTACGGTGATGTGGTACGCCCAGAACCAA
TGACAGTACGTTGGTCACAATATGCACAAAGCCTCACCAACAAAGTGATGAAAGGCATGCTGACAGGACCGGTTACGATT
CTACAATGGTCATTTGTACGTAACGACATTCCACGTTCAACGGTATGTAAACAAATCGGTGTGGCACTGTCCGATGAAGT
GTTAGATTTAGAAAAAGCCGGCATCAAAGTGATTCAAATTGACGAACCGGCGATCCGTGAAGGTTTACCACTGAAACGTG
CAGACTGGGATGCGTACTTACAATGGGCAGGCGAAGCCTTCCGTTTAAGCTACATGGGTGTACAAGATGATACGCAAATT
CACACACACATGTGTTACTCTGAGTTTAACGACATCTTACCTGCGATTGCGGGCTTAGATGCGGATGTGATTACCATTGA
AACCTCACGTTCAGATATGGAATTATTAACCGCCTTCGGTGATTTCAAATATCCAAACGATATTGGTCCGGGCGTGTATG
ATATTCACAGTCCACGTGTGCCAAAAGCAGAAGAAATTGAACGCTTATTACGTAAAGCATTAAATGTAGTACCAAAAGAG
CGTTTATGGGTGAACCCAGACTGTGGTTTAAAAACCCGTGGCTGGCCGGAAACTATCGCTCAATTAGAAGTGATGATGGA
AGTCACCAAAAAACTCCGTGCAGAATTAAACTAA

Upstream 100 bases:

>100_bases
TGATATTGCATAATGGTCTAAAATCCTTCATTCTATATCCATACAGACGTTTAGCCGTCTATTCAGAGAATAACAACATC
AATTTGAGTGAGGTATAACT

Downstream 100 bases:

>100_bases
TTCTCACACCCATTTCTCTTATAACAATAGCAAACCACAACACAACGGCACTTGATTTCAAGTGCCGTTTTTTTATGCAA
AGTAAATTTCATTGACCTTG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 757; Mature: 756

Protein sequence:

>757_residues
MTTFHVAGFPRVGAKRELKFAQERYWRGEIAEQDLLEIAQKLREINWKHQAAANADFVAVADFTFYDHILDLQVATGAIP
ARFGFDSQNLSLNEYFQLARGNQTQFAIEMTKWFDTNYHYLVPEFTKNTEFKANPAHYVQQIREAKALGFKFKPTIVGPL
TFLWLGKEKGEAFNRFELLAKLVPVYVEILNALVAEGAEWIQIDEPALAVDLPTEWIEAYKAVYTTLKEKVKAKLLLATY
FGSVAEHAPLLKGLPVDGLHIDLVRAPAQLAAFEDYNKVLSVGVIDGRNIWRANLNQVLDVVEPLKAKFGENLWIAPSCS
LLHTPYDLEVETQLKANKPELYSWLAFTLQKVQELRVIKTALEQGRGAVQAELDASQAAADARANSKEIHRPEVAERLAN
LPTDADKRKSPFAERIAKQNAWLNLPLLPTTNIGSFPQTVEIRQARAKFKKGELSVADYEAAMKKEIEFVVRRQEELDLD
VLVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVVRPEPMTVRWSQYAQSLTNKVMKGMLTGPVTI
LQWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVQDDTQI
HTHMCYSEFNDILPAIAGLDADVITIETSRSDMELLTAFGDFKYPNDIGPGVYDIHSPRVPKAEEIERLLRKALNVVPKE
RLWVNPDCGLKTRGWPETIAQLEVMMEVTKKLRAELN

Sequences:

>Translated_757_residues
MTTFHVAGFPRVGAKRELKFAQERYWRGEIAEQDLLEIAQKLREINWKHQAAANADFVAVADFTFYDHILDLQVATGAIP
ARFGFDSQNLSLNEYFQLARGNQTQFAIEMTKWFDTNYHYLVPEFTKNTEFKANPAHYVQQIREAKALGFKFKPTIVGPL
TFLWLGKEKGEAFNRFELLAKLVPVYVEILNALVAEGAEWIQIDEPALAVDLPTEWIEAYKAVYTTLKEKVKAKLLLATY
FGSVAEHAPLLKGLPVDGLHIDLVRAPAQLAAFEDYNKVLSVGVIDGRNIWRANLNQVLDVVEPLKAKFGENLWIAPSCS
LLHTPYDLEVETQLKANKPELYSWLAFTLQKVQELRVIKTALEQGRGAVQAELDASQAAADARANSKEIHRPEVAERLAN
LPTDADKRKSPFAERIAKQNAWLNLPLLPTTNIGSFPQTVEIRQARAKFKKGELSVADYEAAMKKEIEFVVRRQEELDLD
VLVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVVRPEPMTVRWSQYAQSLTNKVMKGMLTGPVTI
LQWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVQDDTQI
HTHMCYSEFNDILPAIAGLDADVITIETSRSDMELLTAFGDFKYPNDIGPGVYDIHSPRVPKAEEIERLLRKALNVVPKE
RLWVNPDCGLKTRGWPETIAQLEVMMEVTKKLRAELN
>Mature_756_residues
TTFHVAGFPRVGAKRELKFAQERYWRGEIAEQDLLEIAQKLREINWKHQAAANADFVAVADFTFYDHILDLQVATGAIPA
RFGFDSQNLSLNEYFQLARGNQTQFAIEMTKWFDTNYHYLVPEFTKNTEFKANPAHYVQQIREAKALGFKFKPTIVGPLT
FLWLGKEKGEAFNRFELLAKLVPVYVEILNALVAEGAEWIQIDEPALAVDLPTEWIEAYKAVYTTLKEKVKAKLLLATYF
GSVAEHAPLLKGLPVDGLHIDLVRAPAQLAAFEDYNKVLSVGVIDGRNIWRANLNQVLDVVEPLKAKFGENLWIAPSCSL
LHTPYDLEVETQLKANKPELYSWLAFTLQKVQELRVIKTALEQGRGAVQAELDASQAAADARANSKEIHRPEVAERLANL
PTDADKRKSPFAERIAKQNAWLNLPLLPTTNIGSFPQTVEIRQARAKFKKGELSVADYEAAMKKEIEFVVRRQEELDLDV
LVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVVRPEPMTVRWSQYAQSLTNKVMKGMLTGPVTIL
QWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVQDDTQIH
THMCYSEFNDILPAIAGLDADVITIETSRSDMELLTAFGDFKYPNDIGPGVYDIHSPRVPKAEEIERLLRKALNVVPKER
LWVNPDCGLKTRGWPETIAQLEVMMEVTKKLRAELN

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=756, Percent_Identity=53.8359788359788, Blast_Score=821, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=775, Percent_Identity=46.4516129032258, Blast_Score=662, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_PASMU (P57843)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245357.1
- ProteinModelPortal:   P57843
- SMR:   P57843
- GeneID:   1243767
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0420
- NMPDR:   fig|272843.1.peg.420
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- BioCyc:   PMUL272843:PM0420-MONOMER
- BRENDA:   2.1.1.14
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 85685; Mature: 85554

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFHVAGFPRVGAKRELKFAQERYWRGEIAEQDLLEIAQKLREINWKHQAAANADFVAV
CCEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCEEEE
ADFTFYDHILDLQVATGAIPARFGFDSQNLSLNEYFQLARGNQTQFAIEMTKWFDTNYHY
ECHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHCCCCCEEEEEEEHHHCCCCEE
LVPEFTKNTEFKANPAHYVQQIREAKALGFKFKPTIVGPLTFLWLGKEKGEAFNRFELLA
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCEECCEEEEEECCCCCCHHHHHHHHH
KLVPVYVEILNALVAEGAEWIQIDEPALAVDLPTEWIEAYKAVYTTLKEKVKAKLLLATY
HHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGSVAEHAPLLKGLPVDGLHIDLVRAPAQLAAFEDYNKVLSVGVIDGRNIWRANLNQVLD
HHHHHHHCHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHH
VVEPLKAKFGENLWIAPSCSLLHTPYDLEVETQLKANKPELYSWLAFTLQKVQELRVIKT
HHHHHHHHHCCCEEECCCCCEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
ALEQGRGAVQAELDASQAAADARANSKEIHRPEVAERLANLPTDADKRKSPFAERIAKQN
HHHHCCCCEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHCCHHHHHHHHCC
AWLNLPLLPTTNIGSFPQTVEIRQARAKFKKGELSVADYEAAMKKEIEFVVRRQEELDLD
CEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEE
VLVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVVRPEPMTVRWSQ
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEHHH
YAQSLTNKVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQI
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCEEEEEE
DEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVQDDTQIHTHMCYSEFNDILPAIAGLD
CCHHHHCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
ADVITIETSRSDMELLTAFGDFKYPNDIGPGVYDIHSPRVPKAEEIERLLRKALNVVPKE
CCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCC
RLWVNPDCGLKTRGWPETIAQLEVMMEVTKKLRAELN
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTFHVAGFPRVGAKRELKFAQERYWRGEIAEQDLLEIAQKLREINWKHQAAANADFVAV
CEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCEEEE
ADFTFYDHILDLQVATGAIPARFGFDSQNLSLNEYFQLARGNQTQFAIEMTKWFDTNYHY
ECHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHCCCCCEEEEEEEHHHCCCCEE
LVPEFTKNTEFKANPAHYVQQIREAKALGFKFKPTIVGPLTFLWLGKEKGEAFNRFELLA
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCEECCEEEEEECCCCCCHHHHHHHHH
KLVPVYVEILNALVAEGAEWIQIDEPALAVDLPTEWIEAYKAVYTTLKEKVKAKLLLATY
HHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGSVAEHAPLLKGLPVDGLHIDLVRAPAQLAAFEDYNKVLSVGVIDGRNIWRANLNQVLD
HHHHHHHCHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHH
VVEPLKAKFGENLWIAPSCSLLHTPYDLEVETQLKANKPELYSWLAFTLQKVQELRVIKT
HHHHHHHHHCCCEEECCCCCEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
ALEQGRGAVQAELDASQAAADARANSKEIHRPEVAERLANLPTDADKRKSPFAERIAKQN
HHHHCCCCEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHCCHHHHHHHHCC
AWLNLPLLPTTNIGSFPQTVEIRQARAKFKKGELSVADYEAAMKKEIEFVVRRQEELDLD
CEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEE
VLVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVVRPEPMTVRWSQ
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEHHH
YAQSLTNKVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEKAGIKVIQI
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCEEEEEE
DEPAIREGLPLKRADWDAYLQWAGEAFRLSYMGVQDDTQIHTHMCYSEFNDILPAIAGLD
CCHHHHCCCCCCCCCHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
ADVITIETSRSDMELLTAFGDFKYPNDIGPGVYDIHSPRVPKAEEIERLLRKALNVVPKE
CCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCC
RLWVNPDCGLKTRGWPETIAQLEVMMEVTKKLRAELN
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100