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The map label for this gene is ispB [H]

Identifier: 15602211

GI number: 15602211

Start: 408419

End: 409411

Strand: Reverse

Name: ispB [H]

Synonym: PM0346

Alternate gene names: 15602211

Gene position: 409411-408419 (Counterclockwise)

Preceding gene: 15602217

Following gene: 15602210

Centisome position: 18.14

GC content: 40.38

Gene sequence:

>993_bases
ATGACAAAGCAACATAATTTAATGAGTATTGATGACATCCAAAAACTCATTCATCGTGATATGCAAAAAACCAATGAAAC
AATTCTCGCTCAGCTTAACTCTGATGTCACGTTAATTAATCAATTGGGTTATTATATCATCCAAAGTGGTGGAAAACGTA
TCCGTCCGATGATTGCGATTTTGGCTGCACGTGCACTGGGATTTCATGGTGAACAACCGATTACTTGTGCCACTTTTATT
GAATTTATTCATACCGCGACTTTATTACATGATGATGTAGTAGATGAATCTGCATTACGACGAGGTAATCCCACTGCTAA
CGCGGAATTTGGTAATGCGGCAAGTGTGTTAGTGGGGGATTTTATTTATACTCGCGCTTTCCAATTAATGACGCAATTGG
ACTCTTTACGTATTCTCAAAGTGATGTCAGATGCAACCAATGTCATTGCAGAAGGTGAAGTGCAACAATTAATGAATGTC
AACGATCCGAATACGACGGAAGAAAACTATATGCAAGTCATTTATGGCAAGACTGCGCGTCTATTCGAAGCTGCGACGCA
TTGTGCCGCGATACTTGCTCATGCGACGGAAGCCCAAGAAAATGCTTTACGCGAATATGGTTGTTATCTAGGCACAGCTT
TTCAATTAGTCGATGATATTTTAGATTATAGTGCAGATGCACAAGCACTCGGCAAAAATATTGGTGATGATTTAGCGGAA
GGTAAACCAACATTACCGTTATTGCACGCCATGCATCACGGCAATCCAGCACAAGCAAAATTGATTCGCGAAGCGATTGA
GCAAGGGGGTAAGCGTGATATTTTAGAGGATGTACTGACAATTATGACAGAACATAAATCCCTCGACTATGCGATGATGC
GCGCTAAACAAGAGGCACAAAAAGCCGTTGATGCGATTGCATTATTGCCTGAAAATGAATATAAACAAGCGTTAATTTCA
TTAGCTTACTTATCCGTCGATCGCGCTTATTAA

Upstream 100 bases:

>100_bases
TAAAGTAAAAAAATTATGATTTTGTGTGATTTAATTTCAGAAATCGCAGAAAATTGGGTACAATCCCTTTCACATTCAGT
CTAAAAATCCATTATTACAA

Downstream 100 bases:

>100_bases
CCACTTAATAAGGCGAGACATGTTAGCGTAACGACCGCCTAAAGTGCGGTCATTTATTTAGTAATTTTAAACACGACAAT
GACAGAACAAAACATCCCTA

Product: IspB

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTKQHNLMSIDDIQKLIHRDMQKTNETILAQLNSDVTLINQLGYYIIQSGGKRIRPMIAILAARALGFHGEQPITCATFI
EFIHTATLLHDDVVDESALRRGNPTANAEFGNAASVLVGDFIYTRAFQLMTQLDSLRILKVMSDATNVIAEGEVQQLMNV
NDPNTTEENYMQVIYGKTARLFEAATHCAAILAHATEAQENALREYGCYLGTAFQLVDDILDYSADAQALGKNIGDDLAE
GKPTLPLLHAMHHGNPAQAKLIREAIEQGGKRDILEDVLTIMTEHKSLDYAMMRAKQEAQKAVDAIALLPENEYKQALIS
LAYLSVDRAY

Sequences:

>Translated_330_residues
MTKQHNLMSIDDIQKLIHRDMQKTNETILAQLNSDVTLINQLGYYIIQSGGKRIRPMIAILAARALGFHGEQPITCATFI
EFIHTATLLHDDVVDESALRRGNPTANAEFGNAASVLVGDFIYTRAFQLMTQLDSLRILKVMSDATNVIAEGEVQQLMNV
NDPNTTEENYMQVIYGKTARLFEAATHCAAILAHATEAQENALREYGCYLGTAFQLVDDILDYSADAQALGKNIGDDLAE
GKPTLPLLHAMHHGNPAQAKLIREAIEQGGKRDILEDVLTIMTEHKSLDYAMMRAKQEAQKAVDAIALLPENEYKQALIS
LAYLSVDRAY
>Mature_329_residues
TKQHNLMSIDDIQKLIHRDMQKTNETILAQLNSDVTLINQLGYYIIQSGGKRIRPMIAILAARALGFHGEQPITCATFIE
FIHTATLLHDDVVDESALRRGNPTANAEFGNAASVLVGDFIYTRAFQLMTQLDSLRILKVMSDATNVIAEGEVQQLMNVN
DPNTTEENYMQVIYGKTARLFEAATHCAAILAHATEAQENALREYGCYLGTAFQLVDDILDYSADAQALGKNIGDDLAEG
KPTLPLLHAMHHGNPAQAKLIREAIEQGGKRDILEDVLTIMTEHKSLDYAMMRAKQEAQKAVDAIALLPENEYKQALISL
AYLSVDRAY

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=336, Percent_Identity=27.3809523809524, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI4758430, Length=295, Percent_Identity=24.406779661017, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI83700220, Length=295, Percent_Identity=24.406779661017, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=65.7320872274143, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1786623, Length=245, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=354, Percent_Identity=32.2033898305085, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319475, Length=287, Percent_Identity=31.7073170731707, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325188, Length=224, Percent_Identity=23.6607142857143, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24651612, Length=298, Percent_Identity=29.5302013422819, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI281365769, Length=322, Percent_Identity=24.8447204968944, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24660002, Length=322, Percent_Identity=24.8447204968944, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36546; Mature: 36415

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQHNLMSIDDIQKLIHRDMQKTNETILAQLNSDVTLINQLGYYIIQSGGKRIRPMIAI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHH
LAARALGFHGEQPITCATFIEFIHTATLLHDDVVDESALRRGNPTANAEFGNAASVLVGD
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
FIYTRAFQLMTQLDSLRILKVMSDATNVIAEGEVQQLMNVNDPNTTEENYMQVIYGKTAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LFEAATHCAAILAHATEAQENALREYGCYLGTAFQLVDDILDYSADAQALGKNIGDDLAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHC
GKPTLPLLHAMHHGNPAQAKLIREAIEQGGKRDILEDVLTIMTEHKSLDYAMMRAKQEAQ
CCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KAVDAIALLPENEYKQALISLAYLSVDRAY
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKQHNLMSIDDIQKLIHRDMQKTNETILAQLNSDVTLINQLGYYIIQSGGKRIRPMIAI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHH
LAARALGFHGEQPITCATFIEFIHTATLLHDDVVDESALRRGNPTANAEFGNAASVLVGD
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
FIYTRAFQLMTQLDSLRILKVMSDATNVIAEGEVQQLMNVNDPNTTEENYMQVIYGKTAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LFEAATHCAAILAHATEAQENALREYGCYLGTAFQLVDDILDYSADAQALGKNIGDDLAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHC
GKPTLPLLHAMHHGNPAQAKLIREAIEQGGKRDILEDVLTIMTEHKSLDYAMMRAKQEAQ
CCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KAVDAIALLPENEYKQALISLAYLSVDRAY
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]