Click here to switch to the map view.

The map label for this gene is ppnK

Identifier: 15602198

GI number: 15602198

Start: 387262

End: 388179

Strand: Reverse

Name: ppnK

Synonym: PM0333

Alternate gene names: 15602198

Gene position: 388179-387262 (Counterclockwise)

Preceding gene: 15602201

Following gene: 15602197

Centisome position: 17.2

GC content: 41.5

Gene sequence:

>918_bases
ATGAAACACCCGAATACCATTGATATTAAAGCGCTACAATCTTCTTTTCAGATCATAGGGTTACTTGGTAAACCTCGCCA
TGATGTGACCTTACAAATGCATAAAAATCTTTTTCAATGGTTACTTGAGAAAGGCTATCAGGTGCTCGTTGAGCGACCAA
TTGGTGAACAACTTGGTTTGAGTGAAAATTATTTAGCCTCTGTGGATGAGATTGGACAACAGGCTCAACTGGCCATTGTG
ATTGGTGGCGATGGTAATGTATTAGGTCGTGCTCGAACCCTTGCTAAATATGACATTGCATTAATTGGTATCAATAGAGG
CAACTTAGGCTTTCTCACGGATATCGACCCGAAAAATGCCTATTCACAACTGCAAGCCTGTTTAGAAGACGGGGACTGTT
TTGTAGAAGAACGCTTCATTTTAGAAGCCAGTGTCGAACGAAATGGAAAAATCATTGCGCGCGGAAATGCAGTCAATGAA
GCGGTAGTTCACCCAGCTAAAATTGCCCACATGATCGATTTTCATGTTTATATCAATGATAAATTCGCCTTCTCACAACG
TTCAGACGGTTTAATTATTTCTACACCAACAGGCTCGACTGCCTACTCACTCTCTGCTGGGGGACCTATTTTAACGCCAC
AGCTCAATGCTATTGCACTGGTGCCGATGTTCCCTCACACCCTATCCTCTCGCCCACTGGTGATTGATGGCGACAGCAAA
ATTTCCATTCGTTTCGCAGAATATAATACCTCACAATTAGAAGTCGGTTGTGATAGTCAAGTGGCGTTAGAATTCTCGCC
AGATGACATTGTACATATTCAAAAAAGTCCTGATAAACTGCGTCTGCTACATTTGAAAAATTACAATTACTATAAAGTAT
TGAGTTCCAAATTAGGCTGGCTACGCAATTCCGTTTAA

Upstream 100 bases:

>100_bases
CTATAGATACTTGCTGACAAATAAAAGGATTTGTGTGTAATATAATGACTAATTTTTTGAATTCAAGTGCGGTTATCTTT
TTTAGCCGATGGAATAAGAC

Downstream 100 bases:

>100_bases
AAATGCTTAAAATTTAGCAAAAGCACTTTACTGTATATAAAAACAGTTATAAACTGATAAATATACAGTAAGGGAGATTT
TGCAATGCTTATTCAACTCA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKHPNTIDIKALQSSFQIIGLLGKPRHDVTLQMHKNLFQWLLEKGYQVLVERPIGEQLGLSENYLASVDEIGQQAQLAIV
IGGDGNVLGRARTLAKYDIALIGINRGNLGFLTDIDPKNAYSQLQACLEDGDCFVEERFILEASVERNGKIIARGNAVNE
AVVHPAKIAHMIDFHVYINDKFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPQLNAIALVPMFPHTLSSRPLVIDGDSK
ISIRFAEYNTSQLEVGCDSQVALEFSPDDIVHIQKSPDKLRLLHLKNYNYYKVLSSKLGWLRNSV

Sequences:

>Translated_305_residues
MKHPNTIDIKALQSSFQIIGLLGKPRHDVTLQMHKNLFQWLLEKGYQVLVERPIGEQLGLSENYLASVDEIGQQAQLAIV
IGGDGNVLGRARTLAKYDIALIGINRGNLGFLTDIDPKNAYSQLQACLEDGDCFVEERFILEASVERNGKIIARGNAVNE
AVVHPAKIAHMIDFHVYINDKFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPQLNAIALVPMFPHTLSSRPLVIDGDSK
ISIRFAEYNTSQLEVGCDSQVALEFSPDDIVHIQKSPDKLRLLHLKNYNYYKVLSSKLGWLRNSV
>Mature_305_residues
MKHPNTIDIKALQSSFQIIGLLGKPRHDVTLQMHKNLFQWLLEKGYQVLVERPIGEQLGLSENYLASVDEIGQQAQLAIV
IGGDGNVLGRARTLAKYDIALIGINRGNLGFLTDIDPKNAYSQLQACLEDGDCFVEERFILEASVERNGKIIARGNAVNE
AVVHPAKIAHMIDFHVYINDKFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPQLNAIALVPMFPHTLSSRPLVIDGDSK
ISIRFAEYNTSQLEVGCDSQVALEFSPDDIVHIQKSPDKLRLLHLKNYNYYKVLSSKLGWLRNSV

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=319, Percent_Identity=24.7648902821317, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1788968, Length=289, Percent_Identity=56.7474048442907, Blast_Score=330, Evalue=5e-92,
Organism=Saccharomyces cerevisiae, GI6320794, Length=239, Percent_Identity=30.1255230125523, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6325068, Length=186, Percent_Identity=31.1827956989247, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6322509, Length=238, Percent_Identity=26.0504201680672, Blast_Score=93, Evalue=4e-20,
Organism=Drosophila melanogaster, GI28573832, Length=247, Percent_Identity=29.9595141700405, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28573826, Length=247, Percent_Identity=29.9595141700405, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28573830, Length=247, Percent_Identity=29.9595141700405, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28573828, Length=247, Percent_Identity=29.9595141700405, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI161077047, Length=247, Percent_Identity=29.9595141700405, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_PASMU (Q9CNU2)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245270.1
- ProteinModelPortal:   Q9CNU2
- SMR:   Q9CNU2
- GeneID:   1243680
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0333
- NMPDR:   fig|272843.1.peg.333
- HOGENOM:   HBG713904
- OMA:   FESFMID
- ProtClustDB:   PRK02231
- BioCyc:   PMUL272843:PM0333-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 33711; Mature: 33711

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHPNTIDIKALQSSFQIIGLLGKPRHDVTLQMHKNLFQWLLEKGYQVLVERPIGEQLGL
CCCCCEEEHHHHHCCEEEEEECCCCCCCEEHHHHHHHHHHHHHCCCEEEEECCCCHHCCC
SENYLASVDEIGQQAQLAIVIGGDGNVLGRARTLAKYDIALIGINRGNLGFLTDIDPKNA
CCHHHHHHHHCCCCEEEEEEECCCCCEEHHHHEEEEEEEEEEEECCCCEEEEEECCCHHH
YSQLQACLEDGDCFVEERFILEASVERNGKIIARGNAVNEAVVHPAKIAHMIDFHVYIND
HHHHHHHHHCCCEEEEEEEEEEEECCCCCEEEEECCCCCHHHCCHHHEEEEEEEEEEECC
KFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPQLNAIALVPMFPHTLSSRPLVIDGDSK
EEEECCCCCCEEEECCCCCCEEEECCCCCEECCCCCEEEEEEECCCCCCCCCEEEECCCE
ISIRFAEYNTSQLEVGCDSQVALEFSPDDIVHIQKSPDKLRLLHLKNYNYYKVLSSKLGW
EEEEEEECCCCEEEECCCCEEEEEECCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHH
LRNSV
HHCCC
>Mature Secondary Structure
MKHPNTIDIKALQSSFQIIGLLGKPRHDVTLQMHKNLFQWLLEKGYQVLVERPIGEQLGL
CCCCCEEEHHHHHCCEEEEEECCCCCCCEEHHHHHHHHHHHHHCCCEEEEECCCCHHCCC
SENYLASVDEIGQQAQLAIVIGGDGNVLGRARTLAKYDIALIGINRGNLGFLTDIDPKNA
CCHHHHHHHHCCCCEEEEEEECCCCCEEHHHHEEEEEEEEEEEECCCCEEEEEECCCHHH
YSQLQACLEDGDCFVEERFILEASVERNGKIIARGNAVNEAVVHPAKIAHMIDFHVYIND
HHHHHHHHHCCCEEEEEEEEEEEECCCCCEEEEECCCCCHHHCCHHHEEEEEEEEEEECC
KFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPQLNAIALVPMFPHTLSSRPLVIDGDSK
EEEECCCCCCEEEECCCCCCEEEECCCCCEECCCCCEEEEEEECCCCCCCCCEEEECCCE
ISIRFAEYNTSQLEVGCDSQVALEFSPDDIVHIQKSPDKLRLLHLKNYNYYKVLSSKLGW
EEEEEEECCCCEEEECCCCEEEEEECCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHH
LRNSV
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100