Click here to switch to the map view.

The map label for this gene is nifS_1 [H]

Identifier: 15602183

GI number: 15602183

Start: 374078

End: 375292

Strand: Direct

Name: nifS_1 [H]

Synonym: PM0318

Alternate gene names: 15602183

Gene position: 374078-375292 (Clockwise)

Preceding gene: 15602182

Following gene: 15602184

Centisome position: 16.57

GC content: 40.91

Gene sequence:

>1215_bases
ATGAAATTACCTATTTATCTTGATTACGCAGCGACTTGTCCCGTTGATGAGCGTGTTGCCAAAAAAATGATGGAATATTT
AACGGTAGAGGGCAACTTTGGTAACCCAGCCTCCCGTTCACATAAATTTGGCTGGCAGGCAGAAGAAGCTGTTGATGTGG
CACGTAACTATATTGCGGATTTGATCGGGGCAGATTCACGTGAGATCGTGTTTACGTCTGGTGCAACCGAATCAGACAAT
TTAGCCATTAAAGGGGCTGCGCACTTTTATCAAAGCAAAGGTAAACATATTATTACCTGTAAAACTGAGCATAAAGCTGT
ATTAGATACTTGTCGTCAGCTTGAGCGTGAAGGCTTCGAAGTGACCTATTTAAACCCAAAATCAGATGGTTTAATTGATT
TAGAGGAATTAAAAAATGCGATGCGTGATGACACTATTTTAGTTTCTATTATGCACGTTAATAATGAAATTGGTGTGATT
CAAGACATTGCGGCTATCGGTGAATTATGCCGTGCGCGTAAAATCTTATTCCACGTTGATGCCACACAAAGTGTTGGTAA
ATTGCCGATTAATTTAGCCGAATTAAAAGTGGATTTGATGTCAATGTCTAGTCACAAACTTTACGGACCAAAAGGGATCG
GTGCACTTTATGTTAGCCGTAAGCCACGTGTGCGTTTAGAAGCGATCATTCACGGTGGTGGTCATGAGCGTGGTATGCGT
TCAGGTACCTTACCTGTACACCAAATCGTTGGTATGGGTGAAGCATATCGTATCTGTAAAGAAGAAATGGCAAGCGAAAT
GCCACGTTTAAAAGCATTACGCGATCGCTTATACAATGGCTTAAAAGATATTGAAGAAACTTACGTAAACGGTTCGATGG
AACACCGTTTAGACAGCAACTTAAATATCAGCTTTAACTATGTTGAAGGTGAATCATTAATGATGGCTTTACGTGATATC
GCCGTTTCTTCAGGTTCTGCTTGTACTTCTGCGAGTCTTGAGCCATCTTACGTCTTACGTGCGTTAGGCTTAAACGATGA
ATTAGCACACAGCTCAATTCGTTTCACGTTAGGTCGTTATACTACGGAAGAAGAAATTGATTACACTATCGAATTAGTGA
AAAATGCAGTCGCCAAATTGCGTGAGCTTTCACCACTTTGGGATATGTTTAAAGAAGGGATCGATCTGAATACGATCGAG
TGGACGCACCACTAA

Upstream 100 bases:

>100_bases
CAACCATTGAGTTCCGAGGTGACTTTGATAATCTGATTATTTTAGATAATTAAGCCGACAGCTGTGCGGTAAAAATGAAT
TGTTAGGAGTGAGGAAAGAA

Downstream 100 bases:

>100_bases
TCGGAGAAACATTGCCACAGTCTTTGCCGATTGTTCGGTTTATTGATGTGATTTACGCCGTCTAGAAAGGTAATAGAGGC
AATGTTTTTAGGTTTAATAT

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLPIYLDYAATCPVDERVAKKMMEYLTVEGNFGNPASRSHKFGWQAEEAVDVARNYIADLIGADSREIVFTSGATESDN
LAIKGAAHFYQSKGKHIITCKTEHKAVLDTCRQLEREGFEVTYLNPKSDGLIDLEELKNAMRDDTILVSIMHVNNEIGVI
QDIAAIGELCRARKILFHVDATQSVGKLPINLAELKVDLMSMSSHKLYGPKGIGALYVSRKPRVRLEAIIHGGGHERGMR
SGTLPVHQIVGMGEAYRICKEEMASEMPRLKALRDRLYNGLKDIEETYVNGSMEHRLDSNLNISFNYVEGESLMMALRDI
AVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFTLGRYTTEEEIDYTIELVKNAVAKLRELSPLWDMFKEGIDLNTIE
WTHH

Sequences:

>Translated_404_residues
MKLPIYLDYAATCPVDERVAKKMMEYLTVEGNFGNPASRSHKFGWQAEEAVDVARNYIADLIGADSREIVFTSGATESDN
LAIKGAAHFYQSKGKHIITCKTEHKAVLDTCRQLEREGFEVTYLNPKSDGLIDLEELKNAMRDDTILVSIMHVNNEIGVI
QDIAAIGELCRARKILFHVDATQSVGKLPINLAELKVDLMSMSSHKLYGPKGIGALYVSRKPRVRLEAIIHGGGHERGMR
SGTLPVHQIVGMGEAYRICKEEMASEMPRLKALRDRLYNGLKDIEETYVNGSMEHRLDSNLNISFNYVEGESLMMALRDI
AVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFTLGRYTTEEEIDYTIELVKNAVAKLRELSPLWDMFKEGIDLNTIE
WTHH
>Mature_404_residues
MKLPIYLDYAATCPVDERVAKKMMEYLTVEGNFGNPASRSHKFGWQAEEAVDVARNYIADLIGADSREIVFTSGATESDN
LAIKGAAHFYQSKGKHIITCKTEHKAVLDTCRQLEREGFEVTYLNPKSDGLIDLEELKNAMRDDTILVSIMHVNNEIGVI
QDIAAIGELCRARKILFHVDATQSVGKLPINLAELKVDLMSMSSHKLYGPKGIGALYVSRKPRVRLEAIIHGGGHERGMR
SGTLPVHQIVGMGEAYRICKEEMASEMPRLKALRDRLYNGLKDIEETYVNGSMEHRLDSNLNISFNYVEGESLMMALRDI
AVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFTLGRYTTEEEIDYTIELVKNAVAKLRELSPLWDMFKEGIDLNTIE
WTHH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=57.2139303482587, Blast_Score=501, Evalue=1e-142,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=30.2884615384615, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=80.940594059406, Blast_Score=708, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=224, Percent_Identity=32.5892857142857, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789175, Length=215, Percent_Identity=27.906976744186, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=403, Percent_Identity=57.3200992555831, Blast_Score=483, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17533177, Length=330, Percent_Identity=33.030303030303, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=58.354114713217, Blast_Score=494, Evalue=1e-141,
Organism=Drosophila melanogaster, GI20129463, Length=403, Percent_Identity=56.3275434243176, Blast_Score=476, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45169; Mature: 45169

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYLDYAATCPVDERVAKKMMEYLTVEGNFGNPASRSHKFGWQAEEAVDVARNYIAD
CCCEEEEECCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LIGADSREIVFTSGATESDNLAIKGAAHFYQSKGKHIITCKTEHKAVLDTCRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCE
VTYLNPKSDGLIDLEELKNAMRDDTILVSIMHVNNEIGVIQDIAAIGELCRARKILFHVD
EEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEEEC
ATQSVGKLPINLAELKVDLMSMSSHKLYGPKGIGALYVSRKPRVRLEAIIHGGGHERGMR
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCC
SGTLPVHQIVGMGEAYRICKEEMASEMPRLKALRDRLYNGLKDIEETYVNGSMEHRLDSN
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
LNISFNYVEGESLMMALRDIAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFTLGRY
CCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHCHHEEEECCC
TTEEEIDYTIELVKNAVAKLRELSPLWDMFKEGIDLNTIEWTHH
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEECCC
>Mature Secondary Structure
MKLPIYLDYAATCPVDERVAKKMMEYLTVEGNFGNPASRSHKFGWQAEEAVDVARNYIAD
CCCEEEEECCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LIGADSREIVFTSGATESDNLAIKGAAHFYQSKGKHIITCKTEHKAVLDTCRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCE
VTYLNPKSDGLIDLEELKNAMRDDTILVSIMHVNNEIGVIQDIAAIGELCRARKILFHVD
EEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEEEC
ATQSVGKLPINLAELKVDLMSMSSHKLYGPKGIGALYVSRKPRVRLEAIIHGGGHERGMR
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCC
SGTLPVHQIVGMGEAYRICKEEMASEMPRLKALRDRLYNGLKDIEETYVNGSMEHRLDSN
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
LNISFNYVEGESLMMALRDIAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFTLGRY
CCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHCHHEEEECCC
TTEEEIDYTIELVKNAVAKLRELSPLWDMFKEGIDLNTIEWTHH
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA