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The map label for this gene is lysU_2

Identifier: 15602067

GI number: 15602067

Start: 225736

End: 226707

Strand: Direct

Name: lysU_2

Synonym: PM0202

Alternate gene names: 15602067

Gene position: 225736-226707 (Clockwise)

Preceding gene: 15602062

Following gene: 15602070

Centisome position: 10.0

GC content: 39.4

Gene sequence:

>972_bases
ATGTTTGAACAAGAAAATTGGCAGCCTTCAGCCTCTATTGAGAATTTGCTAGCACGAGCCAAGATTATTGCTGAAATTCG
TCGCTTTTTTACTGATCGTGGTTTATTAGAAGTAGAAACCCCTGTACTCAGTGAATTTGGGGTCACAGATGTGCATTTAT
CGACGTTTAACACTACATTTATTTCACCTACAGCTGAAAAATCGAAAGCATTGTGGTTATCAACTAGCCCAGAATATCAT
ATGAAGCGTTTACTTGCTGCTGGTAGTGGTCCTATTTTTCAGTTATGTCACGTGTTTCGTAACGAAGAAGCTGGGCAACG
TCACAATCCTGAATTTACGATGTTGGAATGGTATCGTCCTCATTTTGATATGTATCGTTTAATTAATGAAGTGGATGATC
TATTGCAGCAAATTTTGGATTGTAAACCAACAGAAAGTTTAAGTTATCAATTTGTTTTCCAAGAATATGTGGGCTTAGAT
CCACTTTCTGCGGAAAAAGCGGAATTAGTGGCAAAGGCAAAGCAATATCATTTACAGCAGGCTGAGCAAGAAGATCGTGA
TACCTTATTGCAATTTTTATTTAGCACTGTTGTTGAGCCGAATATTGGTAAAGAAAATCCGGTCGCAGTTTATCATTTCC
CCGCGACACAAGCCGCACTTGCACAAATCAGCTCTGAAGATCATCGTGTTGCAGAACGTTTTGAGTTTTATTACAAAGGG
CTTGAATTAGCGAATGGTTTCCATGAGTTAACAGATGTGAATGAACAATTGCATCGCTTTGAGCAAGATAATGTACAACG
ACAAAAAATGGGTTTGCCGCAACGTCAAATTGATAAACGCTTATTAGGGGCATTACAAGCGGGAGTGCCGAATTGTTCTG
GTATTGCATTAGGTGTGGACCGCTTATTGATGATTGCCTTAGGCGCAAATGCAATTCATGAAGTCATGGCATTTGGTATC
GAAAACGCTTAA

Upstream 100 bases:

>100_bases
AATGCGTAGATTATTTTCTGTACAAGTGTGAGGCAGAATTTGAAATAACCTAAGGGGATAGTTAGAATAGCGCCATCTTA
TTTATAAAACGGACAAGTTT

Downstream 100 bases:

>100_bases
GCACGACGAGAATAAAAATAGGCAGAACAGTATCTGCCTATTTTTTTAGCTTATTTTGCCATTTTCACTTCTTTGACCCA
TTTGTCTGTGAGACGTTTGC

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MFEQENWQPSASIENLLARAKIIAEIRRFFTDRGLLEVETPVLSEFGVTDVHLSTFNTTFISPTAEKSKALWLSTSPEYH
MKRLLAAGSGPIFQLCHVFRNEEAGQRHNPEFTMLEWYRPHFDMYRLINEVDDLLQQILDCKPTESLSYQFVFQEYVGLD
PLSAEKAELVAKAKQYHLQQAEQEDRDTLLQFLFSTVVEPNIGKENPVAVYHFPATQAALAQISSEDHRVAERFEFYYKG
LELANGFHELTDVNEQLHRFEQDNVQRQKMGLPQRQIDKRLLGALQAGVPNCSGIALGVDRLLMIALGANAIHEVMAFGI
ENA

Sequences:

>Translated_323_residues
MFEQENWQPSASIENLLARAKIIAEIRRFFTDRGLLEVETPVLSEFGVTDVHLSTFNTTFISPTAEKSKALWLSTSPEYH
MKRLLAAGSGPIFQLCHVFRNEEAGQRHNPEFTMLEWYRPHFDMYRLINEVDDLLQQILDCKPTESLSYQFVFQEYVGLD
PLSAEKAELVAKAKQYHLQQAEQEDRDTLLQFLFSTVVEPNIGKENPVAVYHFPATQAALAQISSEDHRVAERFEFYYKG
LELANGFHELTDVNEQLHRFEQDNVQRQKMGLPQRQIDKRLLGALQAGVPNCSGIALGVDRLLMIALGANAIHEVMAFGI
ENA
>Mature_323_residues
MFEQENWQPSASIENLLARAKIIAEIRRFFTDRGLLEVETPVLSEFGVTDVHLSTFNTTFISPTAEKSKALWLSTSPEYH
MKRLLAAGSGPIFQLCHVFRNEEAGQRHNPEFTMLEWYRPHFDMYRLINEVDDLLQQILDCKPTESLSYQFVFQEYVGLD
PLSAEKAELVAKAKQYHLQQAEQEDRDTLLQFLFSTVVEPNIGKENPVAVYHFPATQAALAQISSEDHRVAERFEFYYKG
LELANGFHELTDVNEQLHRFEQDNVQRQKMGLPQRQIDKRLLGALQAGVPNCSGIALGVDRLLMIALGANAIHEVMAFGI
ENA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=65.8461538461538, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1790571, Length=331, Percent_Identity=29.0030211480363, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789256, Length=324, Percent_Identity=29.0123456790123, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71994340, Length=339, Percent_Identity=26.8436578171091, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17535925, Length=339, Percent_Identity=26.8436578171091, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17535927, Length=339, Percent_Identity=26.8436578171091, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6320242, Length=337, Percent_Identity=26.1127596439169, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324256, Length=354, Percent_Identity=27.683615819209, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YJEA_PASMU (P57824)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245139.1
- ProteinModelPortal:   P57824
- SMR:   P57824
- GeneID:   1243549
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0202
- NMPDR:   fig|272843.1.peg.202
- HOGENOM:   HBG631383
- OMA:   MPEASGI
- ProtClustDB:   PRK09350
- BioCyc:   PMUL272843:PM0202-MONOMER
- BRENDA:   6.1.1.6
- GO:   GO:0005737
- HAMAP:   MF_00174
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149
- PANTHER:   PTHR22594
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00462

Pfam domain/function: PF00152 tRNA-synt_2

EC number: 6.1.1.6

Molecular weight: Translated: 36859; Mature: 36859

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEQENWQPSASIENLLARAKIIAEIRRFFTDRGLLEVETPVLSEFGVTDVHLSTFNTTF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCEEEEECCCCEE
ISPTAEKSKALWLSTSPEYHMKRLLAAGSGPIFQLCHVFRNEEAGQRHNPEFTMLEWYRP
ECCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEHHHHCC
HFDMYRLINEVDDLLQQILDCKPTESLSYQFVFQEYVGLDPLSAEKAELVAKAKQYHLQQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
AEQEDRDTLLQFLFSTVVEPNIGKENPVAVYHFPATQAALAQISSEDHRVAERFEFYYKG
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHH
LELANGFHELTDVNEQLHRFEQDNVQRQKMGLPQRQIDKRLLGALQAGVPNCSGIALGVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
RLLMIALGANAIHEVMAFGIENA
HHHHHHHCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFEQENWQPSASIENLLARAKIIAEIRRFFTDRGLLEVETPVLSEFGVTDVHLSTFNTTF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCEEEEECCCCEE
ISPTAEKSKALWLSTSPEYHMKRLLAAGSGPIFQLCHVFRNEEAGQRHNPEFTMLEWYRP
ECCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEHHHHCC
HFDMYRLINEVDDLLQQILDCKPTESLSYQFVFQEYVGLDPLSAEKAELVAKAKQYHLQQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
AEQEDRDTLLQFLFSTVVEPNIGKENPVAVYHFPATQAALAQISSEDHRVAERFEFYYKG
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHH
LELANGFHELTDVNEQLHRFEQDNVQRQKMGLPQRQIDKRLLGALQAGVPNCSGIALGVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
RLLMIALGANAIHEVMAFGIENA
HHHHHHHCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100