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The map label for this gene is ddl

Identifier: 15602009

GI number: 15602009

Start: 170685

End: 171614

Strand: Direct

Name: ddl

Synonym: PM0144

Alternate gene names: 15602009

Gene position: 170685-171614 (Clockwise)

Preceding gene: 15602008

Following gene: 15602010

Centisome position: 7.56

GC content: 41.94

Gene sequence:

>930_bases
ATGAAAAAAACATTAAAACAAGAAAAAATTGCAGTGTTATTAGGGGGCACTTCAGCAGAGCGTGAGGTTTCTTTGGATTC
AGGTCAGGCGGTGCTCAATGCGTTACTGAGTGCTGGCTTTGATGCTCACCCTATCGATCCGAAAACTTTTCCTGTAGCGA
CTTTAAAGGAACAGGGTTTTGATCGCGTTTTTAATATTTTACATGGTCGTGGCGGTGAAGATGGCACCATGCAGGGACTA
TTAGAGCAGATTGGTATTCCCTATACGGGGTGTGGCGTCATGACCTCAGCACTGACAATGGATAAAATGCGAACAAAAAT
GCTGTGGAAAGCTTTTGGTTTGCCAGTTGCTGAGATGGAAATTGTGACAAAAGCCAATGTGGGAGAACTTAATCCTAGTG
CTGTTGTCGAAAAGTTAGGCTTACCTGTAATGGTGAAACCTTCATTAGAAGGCTCTAGTGTAGGGTTAACCAAAGTAAAA
CGGGTGGAAGATCTGAAAAGTGCGGTCGATTTTGCCTTAAAATACGATGATACGGTCTTAATTGAAGAGTGGTTATCCGG
TGCCGAATTTACGGTTCCTGTATTAGATAATGAAGTGCTACCTTCTATTCGTATTGTGCCAGAAGGTGAATTTTATGATT
ATGAGGCGAAGTACATTTCAGATAATACCCAATATTTTTGTCCATCTGGCTTAAGTGCTGAACGTGAAGATGAATTAAAA
CAATTGGTGAAACAAGCTTATGATGTGGTGGGATGCCGTGGTTGGAGCCGTATTGATGTGATGTTAGATGGCAATGATGC
GTTCCGTTTAGTGGAAGTGAATACTAACCCGGGCATGACAAGTCATAGTTTATTTCCTAAATCCGCTGCAACAGTCGGGT
ATTCATTTGAGCAACTGGTTGTGAAGATTTTGGAGTTGAGTGCTGAATGA

Upstream 100 bases:

>100_bases
TTTTACAAGATGGGGATTTAATTTTGGCACAGGGTGCGGGTAATGTGAGTAAAATTGCGCGTCAATTAGCAGAAACTTGG
ACCAAAGAATAGGTGGTACA

Downstream 100 bases:

>100_bases
AAGGCTTAAAACGTAAAACAACACAGGGCGTTAAAAAAAGCAATGAGACCAAGATAGGGATGATAAAGAACCTTAAATTG
GCCTTTTTGCTATGTTGTAT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKKTLKQEKIAVLLGGTSAEREVSLDSGQAVLNALLSAGFDAHPIDPKTFPVATLKEQGFDRVFNILHGRGGEDGTMQGL
LEQIGIPYTGCGVMTSALTMDKMRTKMLWKAFGLPVAEMEIVTKANVGELNPSAVVEKLGLPVMVKPSLEGSSVGLTKVK
RVEDLKSAVDFALKYDDTVLIEEWLSGAEFTVPVLDNEVLPSIRIVPEGEFYDYEAKYISDNTQYFCPSGLSAEREDELK
QLVKQAYDVVGCRGWSRIDVMLDGNDAFRLVEVNTNPGMTSHSLFPKSAATVGYSFEQLVVKILELSAE

Sequences:

>Translated_309_residues
MKKTLKQEKIAVLLGGTSAEREVSLDSGQAVLNALLSAGFDAHPIDPKTFPVATLKEQGFDRVFNILHGRGGEDGTMQGL
LEQIGIPYTGCGVMTSALTMDKMRTKMLWKAFGLPVAEMEIVTKANVGELNPSAVVEKLGLPVMVKPSLEGSSVGLTKVK
RVEDLKSAVDFALKYDDTVLIEEWLSGAEFTVPVLDNEVLPSIRIVPEGEFYDYEAKYISDNTQYFCPSGLSAEREDELK
QLVKQAYDVVGCRGWSRIDVMLDGNDAFRLVEVNTNPGMTSHSLFPKSAATVGYSFEQLVVKILELSAE
>Mature_309_residues
MKKTLKQEKIAVLLGGTSAEREVSLDSGQAVLNALLSAGFDAHPIDPKTFPVATLKEQGFDRVFNILHGRGGEDGTMQGL
LEQIGIPYTGCGVMTSALTMDKMRTKMLWKAFGLPVAEMEIVTKANVGELNPSAVVEKLGLPVMVKPSLEGSSVGLTKVK
RVEDLKSAVDFALKYDDTVLIEEWLSGAEFTVPVLDNEVLPSIRIVPEGEFYDYEAKYISDNTQYFCPSGLSAEREDELK
QLVKQAYDVVGCRGWSRIDVMLDGNDAFRLVEVNTNPGMTSHSLFPKSAATVGYSFEQLVVKILELSAE

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=303, Percent_Identity=60.0660066006601, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1786579, Length=352, Percent_Identity=32.1022727272727, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_PASMU (P57819)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245081.1
- ProteinModelPortal:   P57819
- SMR:   P57819
- PRIDE:   P57819
- GeneID:   1243491
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0144
- NMPDR:   fig|272843.1.peg.144
- HOGENOM:   HBG427092
- OMA:   VPYTGSD
- ProtClustDB:   PRK01372
- BioCyc:   PMUL272843:PM0144-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33776; Mature: 33776

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLKQEKIAVLLGGTSAEREVSLDSGQAVLNALLSAGFDAHPIDPKTFPVATLKEQGF
CCCCCCCCCEEEEECCCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCH
DRVFNILHGRGGEDGTMQGLLEQIGIPYTGCGVMTSALTMDKMRTKMLWKAFGLPVAEME
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEE
IVTKANVGELNPSAVVEKLGLPVMVKPSLEGSSVGLTKVKRVEDLKSAVDFALKYDDTVL
EEECCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEH
IEEWLSGAEFTVPVLDNEVLPSIRIVPEGEFYDYEAKYISDNTQYFCPSGLSAEREDELK
HHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCCEEEEECCCCCEECCCCCCCCHHHHHH
QLVKQAYDVVGCRGWSRIDVMLDGNDAFRLVEVNTNPGMTSHSLFPKSAATVGYSFEQLV
HHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCHHHHCCCHHHHH
VKILELSAE
HHHHHHCCC
>Mature Secondary Structure
MKKTLKQEKIAVLLGGTSAEREVSLDSGQAVLNALLSAGFDAHPIDPKTFPVATLKEQGF
CCCCCCCCCEEEEECCCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCH
DRVFNILHGRGGEDGTMQGLLEQIGIPYTGCGVMTSALTMDKMRTKMLWKAFGLPVAEME
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEE
IVTKANVGELNPSAVVEKLGLPVMVKPSLEGSSVGLTKVKRVEDLKSAVDFALKYDDTVL
EEECCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEH
IEEWLSGAEFTVPVLDNEVLPSIRIVPEGEFYDYEAKYISDNTQYFCPSGLSAEREDELK
HHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCCEEEEECCCCCEECCCCCCCCHHHHHH
QLVKQAYDVVGCRGWSRIDVMLDGNDAFRLVEVNTNPGMTSHSLFPKSAATVGYSFEQLV
HHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCHHHHCCCHHHHH
VKILELSAE
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100