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The map label for this gene is mraW

Identifier: 15601999

GI number: 15601999

Start: 158500

End: 159465

Strand: Direct

Name: mraW

Synonym: PM0134

Alternate gene names: 15601999

Gene position: 158500-159465 (Clockwise)

Preceding gene: 15601998

Following gene: 15602000

Centisome position: 7.02

GC content: 41.72

Gene sequence:

>966_bases
ATGAACGATAAAGATCATTTTTCATCACCTGCGCATATTACTGTCTTACTCAATGAGGCAGTAGCGGGTTTAGCCTTGAA
AGAAAAAGGTATCTATATCGATGGTACATTTGGACGAGGTGGACATTCTCGTTTAATCCTTTCCCGTTTAGCCGATAATG
GGCGTTTGATTGCGATTGATCGCGATCCACGTGCTATTGCTGAAGCCAAGACGATTCAAGATCCCCGTTTTCATATTGAA
CATCGCAGTTTTTCTGACATTCCTCGCATTTGTGAACGATTAAATCTTGTTGGTAAAGTCGATGGGATTTTATTGGATTT
AGGTGTTTCTTCTCCCCAGTTAGATGAAGCAGAACGCGGCTTTAGCTTTATGAAAGATGGTCCGCTTGATATGCGTATGG
ATACCACACAAGGTTTATCGGCAAGTGAGTGGCTGCAACAGGTATCAGAACAAGATTTAGCTTGGGTGTTAAAAACCTTT
GGGGAAGAACGGTTTGCCAAACGCATTGCACAAGCGATTGTGAATTATAACAAACAGGCGTTACAACATGGCACGGAACC
ATTGAGCAGAACCTTGCAATTAGCAGAGTTGATTGCCAATGCGGTGCCTTTTAAAGATAAACATAAGCATCCTGCGACAC
GAAGTTTTCAGGCGATTCGGATTTTTATTAATGCAGAATTAGATGAACTCGAGAGCGTGTTGAAATCAGCTGTACAAGTG
CTCGCGCCACAAGGACGATTAGCAATTATCAGTTTTCATTCGTTAGAAGATCGCATGGTGAAACACTTTATGCGTAAGCA
AAGTAAGGGAGAAGATATTCCTAAAGGTTTGCCTTTACGTGATGATCAGATTCAGCGGACACAAACTTTAAAAGTGATTG
GTAAAGCAATTATGCCAACAGAGCAAGAACAACAACAAAATCCGCGTTCTAGAAGTGCAGTATTACGTGTAGCGGAGAAG
TTGTGA

Upstream 100 bases:

>100_bases
TAGAACAGCAGCACTAGCAAAAGGTAAGCGGTGCTAGGCACCGCCTTATCTTCTCTTAATTATTTTAATTTCCTTTTATT
TGTACCGTTGGTAAATAGTT

Downstream 100 bases:

>100_bases
TGTTAGAGAGTCAAGATCGTTATCCATTACAAAATATTATTTTAGATGATTTGTTTAGTGCTAATAAAACTATCATTGCT
TTATTATTTTTGATTGTGAT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNDKDHFSSPAHITVLLNEAVAGLALKEKGIYIDGTFGRGGHSRLILSRLADNGRLIAIDRDPRAIAEAKTIQDPRFHIE
HRSFSDIPRICERLNLVGKVDGILLDLGVSSPQLDEAERGFSFMKDGPLDMRMDTTQGLSASEWLQQVSEQDLAWVLKTF
GEERFAKRIAQAIVNYNKQALQHGTEPLSRTLQLAELIANAVPFKDKHKHPATRSFQAIRIFINAELDELESVLKSAVQV
LAPQGRLAIISFHSLEDRMVKHFMRKQSKGEDIPKGLPLRDDQIQRTQTLKVIGKAIMPTEQEQQQNPRSRSAVLRVAEK
L

Sequences:

>Translated_321_residues
MNDKDHFSSPAHITVLLNEAVAGLALKEKGIYIDGTFGRGGHSRLILSRLADNGRLIAIDRDPRAIAEAKTIQDPRFHIE
HRSFSDIPRICERLNLVGKVDGILLDLGVSSPQLDEAERGFSFMKDGPLDMRMDTTQGLSASEWLQQVSEQDLAWVLKTF
GEERFAKRIAQAIVNYNKQALQHGTEPLSRTLQLAELIANAVPFKDKHKHPATRSFQAIRIFINAELDELESVLKSAVQV
LAPQGRLAIISFHSLEDRMVKHFMRKQSKGEDIPKGLPLRDDQIQRTQTLKVIGKAIMPTEQEQQQNPRSRSAVLRVAEK
L
>Mature_321_residues
MNDKDHFSSPAHITVLLNEAVAGLALKEKGIYIDGTFGRGGHSRLILSRLADNGRLIAIDRDPRAIAEAKTIQDPRFHIE
HRSFSDIPRICERLNLVGKVDGILLDLGVSSPQLDEAERGFSFMKDGPLDMRMDTTQGLSASEWLQQVSEQDLAWVLKTF
GEERFAKRIAQAIVNYNKQALQHGTEPLSRTLQLAELIANAVPFKDKHKHPATRSFQAIRIFINAELDELESVLKSAVQV
LAPQGRLAIISFHSLEDRMVKHFMRKQSKGEDIPKGLPLRDDQIQRTQTLKVIGKAIMPTEQEQQQNPRSRSAVLRVAEK
L

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=346, Percent_Identity=39.3063583815029, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI165377202, Length=196, Percent_Identity=39.7959183673469, Blast_Score=124, Evalue=9e-29,
Organism=Escherichia coli, GI1786270, Length=311, Percent_Identity=63.0225080385852, Blast_Score=406, Evalue=1e-115,
Organism=Drosophila melanogaster, GI62472493, Length=339, Percent_Identity=37.1681415929204, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI28571637, Length=339, Percent_Identity=37.1681415929204, Blast_Score=181, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_PASMU (Q9CPB4)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245071.1
- ProteinModelPortal:   Q9CPB4
- SMR:   Q9CPB4
- GeneID:   1243481
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0134
- NMPDR:   fig|272843.1.peg.134
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   PMUL272843:PM0134-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 36076; Mature: 36076

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: BINDING 60-60 BINDING 84-84 BINDING 106-106 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKDHFSSPAHITVLLNEAVAGLALKEKGIYIDGTFGRGGHSRLILSRLADNGRLIAID
CCCCCCCCCCCEEEEEEHHHHHHHEEECCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEC
RDPRAIAEAKTIQDPRFHIEHRSFSDIPRICERLNLVGKVDGILLDLGVSSPQLDEAERG
CCCHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHC
FSFMKDGPLDMRMDTTQGLSASEWLQQVSEQDLAWVLKTFGEERFAKRIAQAIVNYNKQA
CHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LQHGTEPLSRTLQLAELIANAVPFKDKHKHPATRSFQAIRIFINAELDELESVLKSAVQV
HHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHH
LAPQGRLAIISFHSLEDRMVKHFMRKQSKGEDIPKGLPLRDDQIQRTQTLKVIGKAIMPT
HCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
EQEQQQNPRSRSAVLRVAEKL
CHHHHCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MNDKDHFSSPAHITVLLNEAVAGLALKEKGIYIDGTFGRGGHSRLILSRLADNGRLIAID
CCCCCCCCCCCEEEEEEHHHHHHHEEECCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEC
RDPRAIAEAKTIQDPRFHIEHRSFSDIPRICERLNLVGKVDGILLDLGVSSPQLDEAERG
CCCHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHC
FSFMKDGPLDMRMDTTQGLSASEWLQQVSEQDLAWVLKTFGEERFAKRIAQAIVNYNKQA
CHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LQHGTEPLSRTLQLAELIANAVPFKDKHKHPATRSFQAIRIFINAELDELESVLKSAVQV
HHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHH
LAPQGRLAIISFHSLEDRMVKHFMRKQSKGEDIPKGLPLRDDQIQRTQTLKVIGKAIMPT
HCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
EQEQQQNPRSRSAVLRVAEKL
CHHHHCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100