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The map label for this gene is pfkA [H]

Identifier: 15601934

GI number: 15601934

Start: 95079

End: 96044

Strand: Direct

Name: pfkA [H]

Synonym: PM0069

Alternate gene names: 15601934

Gene position: 95079-96044 (Clockwise)

Preceding gene: 15601933

Following gene: 15601939

Centisome position: 4.21

GC content: 42.65

Gene sequence:

>966_bases
ATGATAAAAAAAATAGCAGTATTAACCAGTGGTGGTGACGCACCGGGCATGAATGCCGCCATTCGCGGCGTGGTTCGTTC
AGCACTTGCGGCAGGATTAGAAGTATACGGTATTTATGAAGGCTATTATGGGTTATATCATAATAAAGTGAAACAAATGA
CCCGTTATAGTGTTTCCGACATTATTAACCGCGGCGGTACTTTTTTAGGATCGGCGCGTTTTCCTGAATTTAAAGATCCT
GCGGTTCGCGCTAAATGTGCTGAAATTTTACGTTCCCATGGTATTGATGCACTTGTGGTGATTGGTGGGGACGGTTCTTA
CATGGGGGCAAAATTACTGACGGAAGAACATGGTTTCCCTTGCGTCGGTATCCCGGGTACGATTGATAATGATGTGGCAG
GGACAGACTACACCATCGGTTATCAAACCGCATTACAGACAGCCGTCGAAGCCATTGACCGTTTACGCGATACCTCAAGC
TCGCACCAACGTATTTCGATTGTTGAAATCATGGGACGTCATTGTAGTGATTTAGCTATTTCAGCGGGTATTGCTGGTGG
TTGTGAGTATATTGTGGCATCGGAAGTAGAATTTAACCGTGAAGAGTTAATTCAACAGATTGAGCGCAGTATTATCAAAG
GTAAACGTCATGCCATTATTGCAATTACTGAGTTAATTTGTGATGTGAATGAATTAGCCCGTGAAATTGAATCGCGTGTG
AAACATGAAACCCGTGCTACCATTTTAGGTCATATTCAACGTGGCGGTACGCCTTGCGCATTTGACCGTATTTTAGGTTC
ACGTATGGGCGTTTATGCGGTTGATTTATTAATGCAAGGTAAAGGTGGCTATTGCGTTGGTATTCAAAATGAACAATTAG
TTCACCACGATATTATTGATGCAATCAACAATATGCGCCGTGAATTTAAAGCGGATTGGTTAGCATTATCGAAACGTTTA
GACTAA

Upstream 100 bases:

>100_bases
ATGTAGTAGGATTTTTCATAAATTTTGCTAGAGCAGTTTGTATGAATAGTGTAATCTTAGCGACGATTTTGGATTCATTT
TTATTTAGAAGGTTTAATAT

Downstream 100 bases:

>100_bases
TCTGTTGAATGATATTGAAGGAAAAACGGTGGAAGACTCCACCGTTTTTTTATGTCCTTAAAAAATCGGTCTTGTAAGAC
AGATAAACACATTTATTTTC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MIKKIAVLTSGGDAPGMNAAIRGVVRSALAAGLEVYGIYEGYYGLYHNKVKQMTRYSVSDIINRGGTFLGSARFPEFKDP
AVRAKCAEILRSHGIDALVVIGGDGSYMGAKLLTEEHGFPCVGIPGTIDNDVAGTDYTIGYQTALQTAVEAIDRLRDTSS
SHQRISIVEIMGRHCSDLAISAGIAGGCEYIVASEVEFNREELIQQIERSIIKGKRHAIIAITELICDVNELAREIESRV
KHETRATILGHIQRGGTPCAFDRILGSRMGVYAVDLLMQGKGGYCVGIQNEQLVHHDIIDAINNMRREFKADWLALSKRL
D

Sequences:

>Translated_321_residues
MIKKIAVLTSGGDAPGMNAAIRGVVRSALAAGLEVYGIYEGYYGLYHNKVKQMTRYSVSDIINRGGTFLGSARFPEFKDP
AVRAKCAEILRSHGIDALVVIGGDGSYMGAKLLTEEHGFPCVGIPGTIDNDVAGTDYTIGYQTALQTAVEAIDRLRDTSS
SHQRISIVEIMGRHCSDLAISAGIAGGCEYIVASEVEFNREELIQQIERSIIKGKRHAIIAITELICDVNELAREIESRV
KHETRATILGHIQRGGTPCAFDRILGSRMGVYAVDLLMQGKGGYCVGIQNEQLVHHDIIDAINNMRREFKADWLALSKRL
D
>Mature_321_residues
MIKKIAVLTSGGDAPGMNAAIRGVVRSALAAGLEVYGIYEGYYGLYHNKVKQMTRYSVSDIINRGGTFLGSARFPEFKDP
AVRAKCAEILRSHGIDALVVIGGDGSYMGAKLLTEEHGFPCVGIPGTIDNDVAGTDYTIGYQTALQTAVEAIDRLRDTSS
SHQRISIVEIMGRHCSDLAISAGIAGGCEYIVASEVEFNREELIQQIERSIIKGKRHAIIAITELICDVNELAREIESRV
KHETRATILGHIQRGGTPCAFDRILGSRMGVYAVDLLMQGKGGYCVGIQNEQLVHHDIIDAINNMRREFKADWLALSKRL
D

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453619, Length=312, Percent_Identity=40.3846153846154, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI266453768, Length=312, Percent_Identity=40.3846153846154, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI266453748, Length=312, Percent_Identity=40.3846153846154, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI4505749, Length=312, Percent_Identity=40.3846153846154, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI11321601, Length=311, Percent_Identity=40.5144694533762, Blast_Score=204, Evalue=7e-53,
Organism=Homo sapiens, GI48762920, Length=313, Percent_Identity=41.5335463258786, Blast_Score=203, Evalue=1e-52,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=71.875, Blast_Score=478, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI25147584, Length=311, Percent_Identity=39.871382636656, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17558788, Length=309, Percent_Identity=39.8058252427184, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI72003601, Length=290, Percent_Identity=31.3793103448276, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321679, Length=340, Percent_Identity=38.5294117647059, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6323861, Length=341, Percent_Identity=34.3108504398827, Blast_Score=179, Evalue=4e-46,
Organism=Drosophila melanogaster, GI28573326, Length=311, Percent_Identity=42.443729903537, Blast_Score=214, Evalue=5e-56,
Organism=Drosophila melanogaster, GI24652337, Length=311, Percent_Identity=41.4790996784566, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI17647809, Length=311, Percent_Identity=41.4790996784566, Blast_Score=210, Evalue=9e-55,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35026; Mature: 35026

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKIAVLTSGGDAPGMNAAIRGVVRSALAAGLEVYGIYEGYYGLYHNKVKQMTRYSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
IINRGGTFLGSARFPEFKDPAVRAKCAEILRSHGIDALVVIGGDGSYMGAKLLTEEHGFP
HHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCC
CVGIPGTIDNDVAGTDYTIGYQTALQTAVEAIDRLRDTSSSHQRISIVEIMGRHCSDLAI
EECCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCHHHHHH
SAGIAGGCEYIVASEVEFNREELIQQIERSIIKGKRHAIIAITELICDVNELAREIESRV
HCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KHETRATILGHIQRGGTPCAFDRILGSRMGVYAVDLLMQGKGGYCVGIQNEQLVHHDIID
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHEECCCCEEEECCCCCHHHHHHHH
AINNMRREFKADWLALSKRLD
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKKIAVLTSGGDAPGMNAAIRGVVRSALAAGLEVYGIYEGYYGLYHNKVKQMTRYSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
IINRGGTFLGSARFPEFKDPAVRAKCAEILRSHGIDALVVIGGDGSYMGAKLLTEEHGFP
HHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCC
CVGIPGTIDNDVAGTDYTIGYQTALQTAVEAIDRLRDTSSSHQRISIVEIMGRHCSDLAI
EECCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCHHHHHH
SAGIAGGCEYIVASEVEFNREELIQQIERSIIKGKRHAIIAITELICDVNELAREIESRV
HCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KHETRATILGHIQRGGTPCAFDRILGSRMGVYAVDLLMQGKGGYCVGIQNEQLVHHDIID
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHEECCCCEEEECCCCCHHHHHHHH
AINNMRREFKADWLALSKRLD
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA