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The map label for this gene is trmE

Identifier: 15674224

GI number: 15674224

Start: 2327749

End: 2329116

Strand: Direct

Name: trmE

Synonym: L0157

Alternate gene names: 15674224

Gene position: 2327749-2329116 (Clockwise)

Preceding gene: 15674221

Following gene: 15674230

Centisome position: 98.4

GC content: 38.08

Gene sequence:

>1368_bases
ATGACATTAACACAAGAATTTGATACGATTGCGGCGATTTCGACACCGCTTGGTGAAGGAGCAATTGCCATTGTTCGTTT
ATCAGGGACAGATGCCCTAAAAATTGCTCAATCTGTCTATAAAGGAAAAAATTTAACTCAAGTTGCTAGCCATACGATTA
ATTATGGCCATATTTTTGAAGAAAAACGTCTCGTTGACGAAGTCATGGTTTCAGTCATGCGTGCGCCTAAAACATTTACA
CGCGAGGATATTGTTGAAATAAATACGCATGGTGGAATTGCAGTCACACAAGAAATTTTGCAATTACTTTTACGTAATGG
CGCAAGACTCGCTGAACCTGGCGAATTTACCAAACGTGCCTTTTTAAATGGACGAATTGACTTGGCTCAGGCTGAATCTG
TCATGGATTTAATTCGAGCTAAAACTGACAAAGCTGCCAACATTGCCGTAAAACAACTTGACGGAAGTCTTTCAAAAATG
ATTAATAACATTCGTCAAGAGATTCTCGAAAGTTTGGCACAAGTCGAGGTCAATATTGACTATCCAGAGTATGATGATGT
GGAAACAATGACTTCACAAATGCTCCTTGAAAAAACGGCTCATTTTGAACAGCTTTTGGAAAATCTTTTATCAACAGCTA
AACGCGGAAAAATTTTAAGAGAAGGTTTAAAAACGGCGATTATTGGTCGGCCAAACGTTGGAAAATCAAGTTTACTTAAT
CAATTGCTTCGTGAAGAAAAAGCCATTGTCACTGATATTGCTGGAACAACGCGCGATGTCATTACTGAATTTGCCAATAT
TGGCGGTGTTCCTCTTGAACTAGTGGATACTGCTGGTATTCGTGAAACAGATGATTTAGTCGAAGCGATTGGGGTTGAAC
GTTCTAAAAAAGCTTTGGCTGAAGCTGATTTAGTTTTACTCGTTTTAGATGCCTCAAATGAATTGACTGACAAAGATTTA
GAGCTCTTAGAACTGTCTAAAAATTCAAATCGCATTGTTCTACTCAACAAAACCGACCTGCCAGAAAAAATTGATAGCAC
TCAAATTTCTGGTGATTTTATCCGAATTTCAGCTCTTAAAAATGAAAACTTAAATGCTGTTGAAGAAAAAATCAACCAAA
TTTTCTTTGCCGGTGAAATTGAAGCCAAAGATGCAACGGTATTATCTAATGCCCGCCACATTTCAATTGTTGAAGAAGCT
TTGAAGGCTCTTAAAGAAGCAAACTCTGGGCTTGCTCTGGGCTTACCTGTTGATTTAATTCAAGTGGATGTCACACGTTG
TTGGCAATTACTTGGAGAAATCACAGGGGAAGCTGCCCCAGATGAATTGATTACACAACTTTTTTCACAATTTTGTTTGG
GAAAATAA

Upstream 100 bases:

>100_bases
TTTATTGGTGCAAACTGAATTATTATAGATTTTTATTTTTTATAAAAAAATAAATTAAATAGGCTAGGAAATTCAGCCTT
TTTGCGTTAAAATAGTCTTT

Downstream 100 bases:

>100_bases
AAAAGAGTTCAAATGAACTCTTTTTTTTACTGACAGAAATTGGTCAATCATCAAATCATCCCTTTAATTTCTGTCAGACA
TTTTCTAACAAACTGGTCAG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTLTQEFDTIAAISTPLGEGAIAIVRLSGTDALKIAQSVYKGKNLTQVASHTINYGHIFEEKRLVDEVMVSVMRAPKTFT
REDIVEINTHGGIAVTQEILQLLLRNGARLAEPGEFTKRAFLNGRIDLAQAESVMDLIRAKTDKAANIAVKQLDGSLSKM
INNIRQEILESLAQVEVNIDYPEYDDVETMTSQMLLEKTAHFEQLLENLLSTAKRGKILREGLKTAIIGRPNVGKSSLLN
QLLREEKAIVTDIAGTTRDVITEFANIGGVPLELVDTAGIRETDDLVEAIGVERSKKALAEADLVLLVLDASNELTDKDL
ELLELSKNSNRIVLLNKTDLPEKIDSTQISGDFIRISALKNENLNAVEEKINQIFFAGEIEAKDATVLSNARHISIVEEA
LKALKEANSGLALGLPVDLIQVDVTRCWQLLGEITGEAAPDELITQLFSQFCLGK

Sequences:

>Translated_455_residues
MTLTQEFDTIAAISTPLGEGAIAIVRLSGTDALKIAQSVYKGKNLTQVASHTINYGHIFEEKRLVDEVMVSVMRAPKTFT
REDIVEINTHGGIAVTQEILQLLLRNGARLAEPGEFTKRAFLNGRIDLAQAESVMDLIRAKTDKAANIAVKQLDGSLSKM
INNIRQEILESLAQVEVNIDYPEYDDVETMTSQMLLEKTAHFEQLLENLLSTAKRGKILREGLKTAIIGRPNVGKSSLLN
QLLREEKAIVTDIAGTTRDVITEFANIGGVPLELVDTAGIRETDDLVEAIGVERSKKALAEADLVLLVLDASNELTDKDL
ELLELSKNSNRIVLLNKTDLPEKIDSTQISGDFIRISALKNENLNAVEEKINQIFFAGEIEAKDATVLSNARHISIVEEA
LKALKEANSGLALGLPVDLIQVDVTRCWQLLGEITGEAAPDELITQLFSQFCLGK
>Mature_454_residues
TLTQEFDTIAAISTPLGEGAIAIVRLSGTDALKIAQSVYKGKNLTQVASHTINYGHIFEEKRLVDEVMVSVMRAPKTFTR
EDIVEINTHGGIAVTQEILQLLLRNGARLAEPGEFTKRAFLNGRIDLAQAESVMDLIRAKTDKAANIAVKQLDGSLSKMI
NNIRQEILESLAQVEVNIDYPEYDDVETMTSQMLLEKTAHFEQLLENLLSTAKRGKILREGLKTAIIGRPNVGKSSLLNQ
LLREEKAIVTDIAGTTRDVITEFANIGGVPLELVDTAGIRETDDLVEAIGVERSKKALAEADLVLLVLDASNELTDKDLE
LLELSKNSNRIVLLNKTDLPEKIDSTQISGDFIRISALKNENLNAVEEKINQIFFAGEIEAKDATVLSNARHISIVEEAL
KALKEANSGLALGLPVDLIQVDVTRCWQLLGEITGEAAPDELITQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=464, Percent_Identity=33.4051724137931, Blast_Score=209, Evalue=5e-54,
Organism=Homo sapiens, GI193082988, Length=495, Percent_Identity=31.3131313131313, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI193082993, Length=454, Percent_Identity=32.3788546255507, Blast_Score=191, Evalue=9e-49,
Organism=Escherichia coli, GI2367268, Length=457, Percent_Identity=35.0109409190372, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI87082120, Length=170, Percent_Identity=37.0588235294118, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17507259, Length=463, Percent_Identity=30.8855291576674, Blast_Score=180, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6323665, Length=490, Percent_Identity=29.3877551020408, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI45550813, Length=477, Percent_Identity=30.6079664570231, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_LACLA (Q9CDH8)

Other databases:

- EMBL:   AE005176
- PIR:   B86905
- RefSeq:   NP_268399.1
- ProteinModelPortal:   Q9CDH8
- SMR:   Q9CDH8
- GeneID:   1115920
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0157
- NMPDR:   fig|272623.1.peg.2297
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   LLAC272623:L0157-MONOMER
- GO:   GO:0005737
- GO:   GO:0016020
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR003661
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- SMART:   SM00388
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49878; Mature: 49747

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: BINDING 26-26 BINDING 86-86 BINDING 125-125 BINDING 455-455

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTQEFDTIAAISTPLGEGAIAIVRLSGTDALKIAQSVYKGKNLTQVASHTINYGHIFE
CCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHH
EKRLVDEVMVSVMRAPKTFTREDIVEINTHGGIAVTQEILQLLLRNGARLAEPGEFTKRA
HHHHHHHHHHHHHHCCCCCCHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
FLNGRIDLAQAESVMDLIRAKTDKAANIAVKQLDGSLSKMINNIRQEILESLAQVEVNID
HHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHEEEECC
YPEYDDVETMTSQMLLEKTAHFEQLLENLLSTAKRGKILREGLKTAIIGRPNVGKSSLLN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHH
QLLREEKAIVTDIAGTTRDVITEFANIGGVPLELVDTAGIRETDDLVEAIGVERSKKALA
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEHHCCCCCCCHHHHHHHHCCCHHHHHHH
EADLVLLVLDASNELTDKDLELLELSKNSNRIVLLNKTDLPEKIDSTQISGDFIRISALK
HCCEEEEEEECCCCCCCCCHHHHHCCCCCCEEEEEECCCCCCHHCCCCCCCCEEEEEEEC
NENLNAVEEKINQIFFAGEIEAKDATVLSNARHISIVEEALKALKEANSGLALGLPVDLI
CCCCHHHHHHHHHEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHH
QVDVTRCWQLLGEITGEAAPDELITQLFSQFCLGK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLTQEFDTIAAISTPLGEGAIAIVRLSGTDALKIAQSVYKGKNLTQVASHTINYGHIFE
CCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHH
EKRLVDEVMVSVMRAPKTFTREDIVEINTHGGIAVTQEILQLLLRNGARLAEPGEFTKRA
HHHHHHHHHHHHHHCCCCCCHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
FLNGRIDLAQAESVMDLIRAKTDKAANIAVKQLDGSLSKMINNIRQEILESLAQVEVNID
HHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHEEEECC
YPEYDDVETMTSQMLLEKTAHFEQLLENLLSTAKRGKILREGLKTAIIGRPNVGKSSLLN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHH
QLLREEKAIVTDIAGTTRDVITEFANIGGVPLELVDTAGIRETDDLVEAIGVERSKKALA
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEHHCCCCCCCHHHHHHHHCCCHHHHHHH
EADLVLLVLDASNELTDKDLELLELSKNSNRIVLLNKTDLPEKIDSTQISGDFIRISALK
HCCEEEEEEECCCCCCCCCHHHHHCCCCCCEEEEEECCCCCCHHCCCCCCCCEEEEEEEC
NENLNAVEEKINQIFFAGEIEAKDATVLSNARHISIVEEALKALKEANSGLALGLPVDLI
CCCCHHHHHHHHHEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHH
QVDVTRCWQLLGEITGEAAPDELITQLFSQFCLGK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471