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The map label for this gene is rsuA

Identifier: 15674222

GI number: 15674222

Start: 2326078

End: 2326899

Strand: Reverse

Name: rsuA

Synonym: L109527

Alternate gene names: 15674222

Gene position: 2326899-2326078 (Counterclockwise)

Preceding gene: 15674223

Following gene: 15674220

Centisome position: 98.36

GC content: 35.28

Gene sequence:

>822_bases
ATGAGAAAAATTACTGACAGAAATTCTGTCAGTAACTTTTTTTCCATTATTTTACTGACAGACCTGTCAGCAAAATCTGA
TATAATAAAAGACATGAGACTAGATAAATTTTTAGCAGAAAGTGGCCTCGGAAGCCGTAGTGAAGTCAAAATTCTATTGA
AAAAAGGGCGCGTGACAGTTGCTGGGGAAGTTCAGCGAGACGGCAGGTTTCAAGTAGACAAGAATTCTAGACAAGTCGCC
TTTGATGGTGAGATTTTAGATTACCAAGAATATTATTATTACTTATTAAATAAACCGCAAGGTCTTGTTTCTGCAACAAA
AGATAATCTTTTTAAGACAGTTGTTCAATTATTATCGGAAAAAGATTTTCGTGAGGATATTTTTCCAGTCGGACGTTTAG
ATAAAAATACTGAGGGACTTTTAATTTTAACGAATGACGGAGCATTGGGACATGATTTAACTCAACCCAAAAAGCATGTT
GAAAAAGAATACTTTGCGCGAATTGATGGAAAAGTTACTGACGAAATTGTCAATAAATTTGCTGAGGGCTTGACTCTAAA
AAATGGAGAAAAAGCACGACCAGGAGAATTATTCATCGAAAATATTTACGAAGGAGAGAGTCAATCTGGCTGGATTAGTG
AAATTCGTGTGATTATTCATGAAGGAAAATTTCATCAAGTCAAACGGATGTTTGAAGCTGTTGAAATGTACGTTCTCTAT
CTCAAACGCATTCGGATGGGCAATTTAGCATTGGATTCAGATTTAGCGCTAGGAGCCTATCGTCCTTTAACAACCGAAGA
AATAGAAAATTTGAAAAAATAA

Upstream 100 bases:

>100_bases
AAGATGATGATGACAGTTTTGCCAATCAATTGGCAGCATATCCACCGGAAACAATGATTATTTTTAAGCTGACAGATGCA
GATATTTATGGAGAATAAGA

Downstream 100 bases:

>100_bases
AAAGTTACTGATGAAAATTCGAAGGTATCCGTAAGTTAAAAAACAACGGCTATCCTAGAAGTTTCATCAGTAACTTTTTT
AATTTTCGCGATTAATATTG

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MRKITDRNSVSNFFSIILLTDLSAKSDIIKDMRLDKFLAESGLGSRSEVKILLKKGRVTVAGEVQRDGRFQVDKNSRQVA
FDGEILDYQEYYYYLLNKPQGLVSATKDNLFKTVVQLLSEKDFREDIFPVGRLDKNTEGLLILTNDGALGHDLTQPKKHV
EKEYFARIDGKVTDEIVNKFAEGLTLKNGEKARPGELFIENIYEGESQSGWISEIRVIIHEGKFHQVKRMFEAVEMYVLY
LKRIRMGNLALDSDLALGAYRPLTTEEIENLKK

Sequences:

>Translated_273_residues
MRKITDRNSVSNFFSIILLTDLSAKSDIIKDMRLDKFLAESGLGSRSEVKILLKKGRVTVAGEVQRDGRFQVDKNSRQVA
FDGEILDYQEYYYYLLNKPQGLVSATKDNLFKTVVQLLSEKDFREDIFPVGRLDKNTEGLLILTNDGALGHDLTQPKKHV
EKEYFARIDGKVTDEIVNKFAEGLTLKNGEKARPGELFIENIYEGESQSGWISEIRVIIHEGKFHQVKRMFEAVEMYVLY
LKRIRMGNLALDSDLALGAYRPLTTEEIENLKK
>Mature_273_residues
MRKITDRNSVSNFFSIILLTDLSAKSDIIKDMRLDKFLAESGLGSRSEVKILLKKGRVTVAGEVQRDGRFQVDKNSRQVA
FDGEILDYQEYYYYLLNKPQGLVSATKDNLFKTVVQLLSEKDFREDIFPVGRLDKNTEGLLILTNDGALGHDLTQPKKHV
EKEYFARIDGKVTDEIVNKFAEGLTLKNGEKARPGELFIENIYEGESQSGWISEIRVIIHEGKFHQVKRMFEAVEMYVLY
LKRIRMGNLALDSDLALGAYRPLTTEEIENLKK

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=241, Percent_Identity=40.2489626556017, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1787524, Length=234, Percent_Identity=31.1965811965812, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1790453, Length=245, Percent_Identity=30.2040816326531, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=33.1460674157303, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 31247; Mature: 31247

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKITDRNSVSNFFSIILLTDLSAKSDIIKDMRLDKFLAESGLGSRSEVKILLKKGRVTV
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHEEEECCCEEE
AGEVQRDGRFQVDKNSRQVAFDGEILDYQEYYYYLLNKPQGLVSATKDNLFKTVVQLLSE
EEECCCCCCEEECCCCCEEEECCCEECHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHCC
KDFREDIFPVGRLDKNTEGLLILTNDGALGHDLTQPKKHVEKEYFARIDGKVTDEIVNKF
CCHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
AEGLTLKNGEKARPGELFIENIYEGESQSGWISEIRVIIHEGKFHQVKRMFEAVEMYVLY
HHCCEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LKRIRMGNLALDSDLALGAYRPLTTEEIENLKK
HHHHHHCCEEECCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MRKITDRNSVSNFFSIILLTDLSAKSDIIKDMRLDKFLAESGLGSRSEVKILLKKGRVTV
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHEEEECCCEEE
AGEVQRDGRFQVDKNSRQVAFDGEILDYQEYYYYLLNKPQGLVSATKDNLFKTVVQLLSE
EEECCCCCCEEECCCCCEEEECCCEECHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHCC
KDFREDIFPVGRLDKNTEGLLILTNDGALGHDLTQPKKHVEKEYFARIDGKVTDEIVNKF
CCHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
AEGLTLKNGEKARPGELFIENIYEGESQSGWISEIRVIIHEGKFHQVKRMFEAVEMYVLY
HHCCEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LKRIRMGNLALDSDLALGAYRPLTTEEIENLKK
HHHHHHCCEEECCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]