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The map label for this gene is glnA

Identifier: 15674182

GI number: 15674182

Start: 2282309

End: 2283649

Strand: Reverse

Name: glnA

Synonym: L0118

Alternate gene names: 15674182

Gene position: 2283649-2282309 (Counterclockwise)

Preceding gene: 30024074

Following gene: 15674180

Centisome position: 96.54

GC content: 39.52

Gene sequence:

>1341_bases
ATGACAATCACAGCAGCAGACATTCGTCGCGACGTCAAAGAAAAGGATATTAAATTCTTGCGTTTGATGTTTACAGACAT
TCTTGGAACACTTAAAAACGTTGAAGTTCCAGCTACAGATGAACAATTGGACAAATTGTTTGAAAACAAAATGATGTTTG
ACGGTTCATCAATCGAAGGATTCGTTCGTATCAATGAATCAGACATGTATCTTTATCCAGACCTTGACACTTGGATTGTT
TTTCCTTGGGGAGATGAGTATGGTAAAGTGGCAGGTGTAATTTGTGATGTTTACACTCCAGAAGGTGAACCTTTCGCTGG
AGACCCACGCGGTGTCTTGAAACGTAACCTTAAATCAATGGAAAAATTGGGCTTCAAGAGCTTTAACCTTGGACCTGAAC
CAGAGTTCTTCCTTTTCAAACTTAATGAAAATGATGAACCAACACTTGAAGTGAATGATAAAGGTGGTTATTTTGACCTT
GCACCAACTGACCTCGCTGGAAACACAAGACGTGAAATCGTAAATGTATTGACAGACCTTGGTTTTGAAGTTGAAGCTTC
TCACCACGAAGTAGCAATCGGTCAACACGAAATTGACTTTAAATATGCCAATGCTTTGAAAGCTTGTGATAATATTCAAA
TCTTTAAGCTTGTTGTTAAAACAATTGCGCGTAAACATGGTTTACATGCAACGTTTATGGCAAAACCAGTTCACGGAATC
AACGGTTCTGGGATGCATTGTAACATGTCTTTATTTACAGAAGATGGTGCCAATGCCTTTGCTGATCCAACAGGAGATAT
GGGACTTTCAGATGTTGCACATAGCTTTATTGCTGGACTTTTGAAACATGCTTATAACTTTACAGCAATTACAAACCCAA
CTGTTAACTCATATAAACGTTTGGTTCCTGGTTATGAAGCACCCGTTTATGTCGCTTGGGCTGGACGTAACAGATCACCT
TTGATTCGCGTACCTGCTTCACGTGGTTTGTCAACTCGTGTTGAACTTCGTGCGGTTGACCCAACAGCAAATCCATATCT
TGCATTGGCTGTTCTATTGGCAGCTGGTCTTGACGGTGTTGAACATAAATTGGAAGCGCCAGAAGCAATCGAATCAAATA
TTTACGTAATGACAGAAGAAGAACGTAAAGCACATGGTATTACAGATTTGCCATCTACTTTGCACAATGCCGTTAAAGCA
TTGCCTGAAGATACAATTGTGACTGAAGCGCTTGGAGAACATGTTTTGGTTAACTTTGTTGAAGCAAAACGCATTGAATG
GGCAAGCTATGCACAATTCGTATCGCAATGGGAAATTGATAACTATTTAGAACTTTATTAA

Upstream 100 bases:

>100_bases
ACTCCTGATCCCACTCATTTTACCCAACCGCGGATGTAAGTTTAGTTTACTTTATTTGTCAACTAAGTTTACAGCGAAAA
TTTAACTAGGAGAGTAAAGC

Downstream 100 bases:

>100_bases
TTGATATAAAAAATCACTGACAAAGCCTGTCAGTGATTTTTTGGACCATTTAAATATTTTAGTTGTTTTCAGTAATTGTC
AAAACGTTGAAGGCAGTTTT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTITAADIRRDVKEKDIKFLRLMFTDILGTLKNVEVPATDEQLDKLFENKMMFDGSSIEGFVRINESDMYLYPDLDTWIV
FPWGDEYGKVAGVICDVYTPEGEPFAGDPRGVLKRNLKSMEKLGFKSFNLGPEPEFFLFKLNENDEPTLEVNDKGGYFDL
APTDLAGNTRREIVNVLTDLGFEVEASHHEVAIGQHEIDFKYANALKACDNIQIFKLVVKTIARKHGLHATFMAKPVHGI
NGSGMHCNMSLFTEDGANAFADPTGDMGLSDVAHSFIAGLLKHAYNFTAITNPTVNSYKRLVPGYEAPVYVAWAGRNRSP
LIRVPASRGLSTRVELRAVDPTANPYLALAVLLAAGLDGVEHKLEAPEAIESNIYVMTEEERKAHGITDLPSTLHNAVKA
LPEDTIVTEALGEHVLVNFVEAKRIEWASYAQFVSQWEIDNYLELY

Sequences:

>Translated_446_residues
MTITAADIRRDVKEKDIKFLRLMFTDILGTLKNVEVPATDEQLDKLFENKMMFDGSSIEGFVRINESDMYLYPDLDTWIV
FPWGDEYGKVAGVICDVYTPEGEPFAGDPRGVLKRNLKSMEKLGFKSFNLGPEPEFFLFKLNENDEPTLEVNDKGGYFDL
APTDLAGNTRREIVNVLTDLGFEVEASHHEVAIGQHEIDFKYANALKACDNIQIFKLVVKTIARKHGLHATFMAKPVHGI
NGSGMHCNMSLFTEDGANAFADPTGDMGLSDVAHSFIAGLLKHAYNFTAITNPTVNSYKRLVPGYEAPVYVAWAGRNRSP
LIRVPASRGLSTRVELRAVDPTANPYLALAVLLAAGLDGVEHKLEAPEAIESNIYVMTEEERKAHGITDLPSTLHNAVKA
LPEDTIVTEALGEHVLVNFVEAKRIEWASYAQFVSQWEIDNYLELY
>Mature_445_residues
TITAADIRRDVKEKDIKFLRLMFTDILGTLKNVEVPATDEQLDKLFENKMMFDGSSIEGFVRINESDMYLYPDLDTWIVF
PWGDEYGKVAGVICDVYTPEGEPFAGDPRGVLKRNLKSMEKLGFKSFNLGPEPEFFLFKLNENDEPTLEVNDKGGYFDLA
PTDLAGNTRREIVNVLTDLGFEVEASHHEVAIGQHEIDFKYANALKACDNIQIFKLVVKTIARKHGLHATFMAKPVHGIN
GSGMHCNMSLFTEDGANAFADPTGDMGLSDVAHSFIAGLLKHAYNFTAITNPTVNSYKRLVPGYEAPVYVAWAGRNRSPL
IRVPASRGLSTRVELRAVDPTANPYLALAVLLAAGLDGVEHKLEAPEAIESNIYVMTEEERKAHGITDLPSTLHNAVKAL
PEDTIVTEALGEHVLVNFVEAKRIEWASYAQFVSQWEIDNYLELY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family

Homologues:

Organism=Homo sapiens, GI221307485, Length=457, Percent_Identity=27.3522975929978, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1790301, Length=447, Percent_Identity=40.2684563758389, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI87081870, Length=456, Percent_Identity=26.7543859649123, Blast_Score=138, Evalue=8e-34,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): GLNA_LACLA (Q9CDL9)

Other databases:

- EMBL:   AE005176
- PIR:   H86899
- RefSeq:   NP_268357.1
- ProteinModelPortal:   Q9CDL9
- SMR:   Q9CDL9
- PhosSite:   Q9CDL9
- GeneID:   1115878
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0118
- NMPDR:   fig|272623.1.peg.2255
- HOGENOM:   HBG553755
- OMA:   ALIRIPK
- ProtClustDB:   CLSK876973
- BioCyc:   LLAC272623:L0118-MONOMER
- BRENDA:   6.3.1.2
- GO:   GO:0005737
- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- Gene3D:   G3DSA:3.30.590.10
- TIGRFAMs:   TIGR00653

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N; SSF54368 Gln_synt_beta

EC number: =6.3.1.2

Molecular weight: Translated: 49694; Mature: 49563

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00180 GLNA_1; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITAADIRRDVKEKDIKFLRLMFTDILGTLKNVEVPATDEQLDKLFENKMMFDGSSIEG
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCEEECCCCCCE
FVRINESDMYLYPDLDTWIVFPWGDEYGKVAGVICDVYTPEGEPFAGDPRGVLKRNLKSM
EEEECCCCEEEECCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHH
EKLGFKSFNLGPEPEFFLFKLNENDEPTLEVNDKGGYFDLAPTDLAGNTRREIVNVLTDL
HHCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHC
GFEVEASHHEVAIGQHEIDFKYANALKACDNIQIFKLVVKTIARKHGLHATFMAKPVHGI
CCEEECCCCEEEECCEECCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCC
NGSGMHCNMSLFTEDGANAFADPTGDMGLSDVAHSFIAGLLKHAYNFTAITNPTVNSYKR
CCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHH
LVPGYEAPVYVAWAGRNRSPLIRVPASRGLSTRVELRAVDPTANPYLALAVLLAAGLDGV
HCCCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCH
EHKLEAPEAIESNIYVMTEEERKAHGITDLPSTLHNAVKALPEDTIVTEALGEHVLVNFV
HHHCCCCHHHCCCEEEEECCCHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EAKRIEWASYAQFVSQWEIDNYLELY
HHHHCCHHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure 
TITAADIRRDVKEKDIKFLRLMFTDILGTLKNVEVPATDEQLDKLFENKMMFDGSSIEG
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCEEECCCCCCE
FVRINESDMYLYPDLDTWIVFPWGDEYGKVAGVICDVYTPEGEPFAGDPRGVLKRNLKSM
EEEECCCCEEEECCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHH
EKLGFKSFNLGPEPEFFLFKLNENDEPTLEVNDKGGYFDLAPTDLAGNTRREIVNVLTDL
HHCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHC
GFEVEASHHEVAIGQHEIDFKYANALKACDNIQIFKLVVKTIARKHGLHATFMAKPVHGI
CCEEECCCCEEEECCEECCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCC
NGSGMHCNMSLFTEDGANAFADPTGDMGLSDVAHSFIAGLLKHAYNFTAITNPTVNSYKR
CCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHH
LVPGYEAPVYVAWAGRNRSPLIRVPASRGLSTRVELRAVDPTANPYLALAVLLAAGLDGV
HCCCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCH
EHKLEAPEAIESNIYVMTEEERKAHGITDLPSTLHNAVKALPEDTIVTEALGEHVLVNFV
HHHCCCCHHHCCCEEEEECCCHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EAKRIEWASYAQFVSQWEIDNYLELY
HHHHCCHHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471