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The map label for this gene is ssbB

Identifier: 15674172

GI number: 15674172

Start: 2274181

End: 2274681

Strand: Reverse

Name: ssbB

Synonym: L0302

Alternate gene names: 15674172

Gene position: 2274681-2274181 (Counterclockwise)

Preceding gene: 15674173

Following gene: 15674171

Centisome position: 96.16

GC content: 42.51

Gene sequence:

>501_bases
ATGATTAACAATGTTGTATTAGTAGGACGCATTACTCGCGATCCTGAACTTCGATACACCCCTCAAAATCAAGCTGTCGC
AACATTTTCATTGGCTGTAAACCGTCAATTTAAAAATGCGAATGGCGAACGTGAGGCTGATTTCATTAACTGCGTTATTT
GGCGCCAACAAGCTGAGAATTTGGCGAATTGGGCTAAAAAAGGAGCTTTGATTGGGGTAACTGGTCGAATTCAAACACGT
AACTATGAAAATCAACAAGGTCAACGCGTTTATGTGACTGAGGTTGTGGCTGACAGTTTCCAAATGTTGGAAAGTAGATC
TGCTCGTGAAGGTATGGGAGGCGGAACTTCTGCTGGTTCATATTCTGCACCTAGCCAATCTACAAATAATACTCCACGTC
CACAAACGAATAACAATAATGCAACACCGAATTTCGGTCGTGATGCTGATCCGTTTGGTAGCTCACCGATGGAAATTTCG
GATGATGACCTTCCATTCTAA

Upstream 100 bases:

>100_bases
GCGAATTTGACCGTCTTGCTAAGATCAACCTTGACATTCTTCGTCATATGATCGTTAAAGTTGAAGCTTAAGAGCTTTCA
TTTCTAAGGAGAAGTTCATC

Downstream 100 bases:

>100_bases
TAGGGTGAAATAATTGACGCTAAAGCGCCTAATTATTTCACGGAAACCGCTATGGCGGATTTCCTAACCCTCTTACTAGT
TATTATGAAAAGCAGAATCG

Product: single-stranded DNA-binding protein

Products: NA

Alternate protein names: SSB 2; Helix-destabilizing protein 2

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MINNVVLVGRITRDPELRYTPQNQAVATFSLAVNRQFKNANGEREADFINCVIWRQQAENLANWAKKGALIGVTGRIQTR
NYENQQGQRVYVTEVVADSFQMLESRSAREGMGGGTSAGSYSAPSQSTNNTPRPQTNNNNATPNFGRDADPFGSSPMEIS
DDDLPF

Sequences:

>Translated_166_residues
MINNVVLVGRITRDPELRYTPQNQAVATFSLAVNRQFKNANGEREADFINCVIWRQQAENLANWAKKGALIGVTGRIQTR
NYENQQGQRVYVTEVVADSFQMLESRSAREGMGGGTSAGSYSAPSQSTNNTPRPQTNNNNATPNFGRDADPFGSSPMEIS
DDDLPF
>Mature_166_residues
MINNVVLVGRITRDPELRYTPQNQAVATFSLAVNRQFKNANGEREADFINCVIWRQQAENLANWAKKGALIGVTGRIQTR
NYENQQGQRVYVTEVVADSFQMLESRSAREGMGGGTSAGSYSAPSQSTNNTPRPQTNNNNATPNFGRDADPFGSSPMEIS
DDDLPF

Specific function: This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair

COG id: COG0629

COG function: function code L; Single-stranded DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SSB domain

Homologues:

Organism=Escherichia coli, GI1790494, Length=178, Percent_Identity=32.0224719101124, Blast_Score=74, Evalue=4e-15,

Paralogues:

None

Copy number: 1622 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SSB2_LACLA (Q9CDM9)

Other databases:

- EMBL:   AE005176
- PIR:   F86898
- RefSeq:   NP_268347.1
- ProteinModelPortal:   Q9CDM9
- GeneID:   1115868
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0302
- NMPDR:   fig|272623.1.peg.2245
- HOGENOM:   HBG755544
- OMA:   NANNERE
- ProtClustDB:   PRK07275
- BioCyc:   LLAC272623:L0302-MONOMER
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000424
- InterPro:   IPR011344
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR10302
- TIGRFAMs:   TIGR00621

Pfam domain/function: PF00436 SSB; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 18304; Mature: 18304

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50935 SSB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINNVVLVGRITRDPELRYTPQNQAVATFSLAVNRQFKNANGEREADFINCVIWRQQAEN
CCCCEEEEEEECCCCCEEECCCCCEEEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHH
LANWAKKGALIGVTGRIQTRNYENQQGQRVYVTEVVADSFQMLESRSAREGMGGGTSAGS
HHHHHHCCCEEEEECEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCC
YSAPSQSTNNTPRPQTNNNNATPNFGRDADPFGSSPMEISDDDLPF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MINNVVLVGRITRDPELRYTPQNQAVATFSLAVNRQFKNANGEREADFINCVIWRQQAEN
CCCCEEEEEEECCCCCEEECCCCCEEEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHH
LANWAKKGALIGVTGRIQTRNYENQQGQRVYVTEVVADSFQMLESRSAREGMGGGTSAGS
HHHHHHCCCEEEEECEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCC
YSAPSQSTNNTPRPQTNNNNATPNFGRDADPFGSSPMEISDDDLPF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471