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The map label for this gene is mscL

Identifier: 15674083

GI number: 15674083

Start: 2170586

End: 2170954

Strand: Direct

Name: mscL

Synonym: L153973

Alternate gene names: 15674083

Gene position: 2170586-2170954 (Clockwise)

Preceding gene: 15674045

Following gene: 15674094

Centisome position: 91.76

GC content: 31.98

Gene sequence:

>369_bases
ATGTTAAAGGAATTTAAAAACTTTATTTTACGTGGGAACGTATTGGACTTAGCCGTTGGGGTTATCATCGGGGCAGCTTT
TACTGCTTTAGTTAAATCTTTAGTTGATAACTTGATTAATCCGTTAATCGGAATGTTTGTTCAAAGTACTGCTCTTGCTC
ATTTGTCAGTAACTGTTGGAAAAACAAAATTCACTTACGGTGCTTTCTTAAATGATGTCATTAATTTCATCATTACAGCC
TTTGTTATTTTCATTCTTATCAAATTCATCAACAAACTCTTCCCTAAAAAAGAAGAAACTGTCGAAGAACAAAAAAACGA
AGAATTGGAAACTCTCCAAGAAATTCGCGATTTATTGAAAAAACAATAA

Upstream 100 bases:

>100_bases
AGGAATTTACTTGAAATGACAAGAAAGCTTACTTTGTCATCTTTAAACTTTTTGGTATAATTTGTTTGAAGGATTTATAA
ACTATTATATAGGAGGATTT

Downstream 100 bases:

>100_bases
AAAAATTACTGACAGAAAAGCTGTCAGTATTTTTTTATTTTAAATTTTGATTGTGAGGCTTCGATTAAACTTGATTTTGA
GAAAAATAAATCTAAAGAGA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 122; Mature: 122

Protein sequence:

>122_residues
MLKEFKNFILRGNVLDLAVGVIIGAAFTALVKSLVDNLINPLIGMFVQSTALAHLSVTVGKTKFTYGAFLNDVINFIITA
FVIFILIKFINKLFPKKEETVEEQKNEELETLQEIRDLLKKQ

Sequences:

>Translated_122_residues
MLKEFKNFILRGNVLDLAVGVIIGAAFTALVKSLVDNLINPLIGMFVQSTALAHLSVTVGKTKFTYGAFLNDVINFIITA
FVIFILIKFINKLFPKKEETVEEQKNEELETLQEIRDLLKKQ
>Mature_122_residues
MLKEFKNFILRGNVLDLAVGVIIGAAFTALVKSLVDNLINPLIGMFVQSTALAHLSVTVGKTKFTYGAFLNDVINFIITA
FVIFILIKFINKLFPKKEETVEEQKNEELETLQEIRDLLKKQ

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=130, Percent_Identity=45.3846153846154, Blast_Score=102, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_LACLA (Q9CDW0)

Other databases:

- EMBL:   AE005176
- PIR:   E86887
- RefSeq:   NP_268258.1
- ProteinModelPortal:   Q9CDW0
- SMR:   Q9CDW0
- GeneID:   1115778
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L153973
- NMPDR:   fig|272623.1.peg.2156
- HOGENOM:   HBG727604
- OMA:   IQLLTEI
- ProtClustDB:   PRK00567
- BioCyc:   LLAC272623:L153973-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 13759; Mature: 13759

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xfec0500)-; HASH(0xb9e3ad8)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEFKNFILRGNVLDLAVGVIIGAAFTALVKSLVDNLINPLIGMFVQSTALAHLSVTVG
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KTKFTYGAFLNDVINFIITAFVIFILIKFINKLFPKKEETVEEQKNEELETLQEIRDLLK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KQ
CH
>Mature Secondary Structure
MLKEFKNFILRGNVLDLAVGVIIGAAFTALVKSLVDNLINPLIGMFVQSTALAHLSVTVG
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KTKFTYGAFLNDVINFIITAFVIFILIKFINKLFPKKEETVEEQKNEELETLQEIRDLLK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KQ
CH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11337471