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The map label for this gene is radA

Identifier: 15674046

GI number: 15674046

Start: 2149123

End: 2150484

Strand: Reverse

Name: radA

Synonym: L0303

Alternate gene names: 15674046

Gene position: 2150484-2149123 (Counterclockwise)

Preceding gene: 15674047

Following gene: 15674044

Centisome position: 90.91

GC content: 40.01

Gene sequence:

>1362_bases
ATGGCGAAGAAAAAATCATCATTTGTTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGACGTTGTCCAAACTG
TGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTTTCAATGACTGGCGAACGTT
CAAAACCATTGAAGCTAGATGAAGTCGAATTATTTGATACTCCTCGGATTGAAACGGACCTAGATGAATTTAATCGTGTA
TTAGGAGGGGGCGTCGTTCCTGGTTCATTAGTTCTGATTGGGGGAGACCCTGGGATAGGTAAATCAACCTTACTTTTACA
GGTTTCTACTCAATTGGCTTCTAGAGGGCGTGTGCTTTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAAGAG
CTGAGCGTTTAGGAGATATTGACCGTGACTTTTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTTGAG
CGTTTAAAACCTAACTTCTTAATTATTGATTCCATTCAAACAATCATGACACCAGAAATTCAATCAACTCAAGGCTCTGT
AAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACGAATGATATTGCGACTTTTATTGTTGGTCATG
TGACTAAAGAAGGACAGCTGGCTGGTCCAAGGATGTTGGAGCACATGGTTGATACGGTGCTGTATTTTGAAGGAGAACGA
AATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGCTCAACTAATGAAATTGGAATCTTTGAGATGCAGGG
TCATGGCTTGGTCGAAGTGACTAATCCCTCAGAGGTCTTTTTAGAAGAACGTTTAGAGGGCTCAACTGGATCTGCAATTG
TTTGTGCACTTGAAGGAACACGGCCTATACTAGTTGAAATACAAGCACTGACGACACCGACAATGTTTGGAAATGCTAAA
AGGACGACCTCAGGCCTAGATTTTAACCGTGTAAGTTTAATTATGGCGGTGCTTGAGAAGAGAACGGGGCTCTTGATGCA
AAATCAAGATGCCTACCTCAAATCTGCGGGGGGAGTAAAATTGGATGAGCCAGCCATTGACCTCGCGGTTGCGGTTGCTG
TGGCTTCAAGTTATAAAGAACTTCCAACAGATGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACGGGTGAAATTCGT
CGGGTGACCCGAATTGAACAAAGATTAAATGAAGCTGCAAAACTAGGTTTTAAAAAAGTATATGCTCCTAAAAATTCAAT
TGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTAACAACTTTAACTGAATGTTTAAAACTAGTCTTTGGCT
AA

Upstream 100 bases:

>100_bases
AAATGGTAAAAGAATAAAATCCTTAATCAAATAAAACAGCGGATTATCCGCTGTTTTGTTACATTTTAATAAATTTTACG
TATGAATAAGTGGAGGGAAA

Downstream 100 bases:

>100_bases
TATAAAAACAAGGCTATTTCATTAATGAAATAGCCTTTGCTTTTTATGAATGACTTATCTTATAAGAATAATAAGCTAAT
AGAATTAATAGACTAGAGCC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Sequences:

>Translated_453_residues
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG
>Mature_452_residues
AKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRVL
GGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVER
LKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGERN
NTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAKR
TTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIRR
VTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=457, Percent_Identity=43.1072210065646, Blast_Score=382, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49894; Mature: 49763

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLD
CCCCCCCCHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
EVELFDTPRIETDLDEFNRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLY
CEEECCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEEHHHHHCCCEEE
VSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVERLKPNFLIIDSIQTIMTPEI
EECCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEECHHHHCCCCC
QSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEHEECCCCCCCCCCHHHHHHHHHHHEEEECCC
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGT
CCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCHHHHHHHHCCCCCCCEEEEEECCC
RPILVEIQALTTPTMFGNAKRTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVK
CEEEEEEEEECCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHCCCCCE
LDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIRRVTRIEQRLNEAAKLGFKKV
ECCCHHHHHHHHHHHHHHHHCCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHE
YAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG
ECCCCCEEECCCCCCEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure 
AKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLD
CCCCCCCHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
EVELFDTPRIETDLDEFNRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLY
CEEECCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEEHHHHHCCCEEE
VSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVERLKPNFLIIDSIQTIMTPEI
EECCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEECHHHHCCCCC
QSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEHEECCCCCCCCCCHHHHHHHHHHHEEEECCC
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGT
CCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCHHHHHHHHCCCCCCCEEEEEECCC
RPILVEIQALTTPTMFGNAKRTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVK
CEEEEEEEEECCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHCCCCCE
LDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIRRVTRIEQRLNEAAKLGFKKV
ECCCHHHHHHHHHHHHHHHHCCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHE
YAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG
ECCCCCEEECCCCCCEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]