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The map label for this gene is gltX

Identifier: 15674035

GI number: 15674035

Start: 2140195

End: 2141646

Strand: Reverse

Name: gltX

Synonym: L0349

Alternate gene names: 15674035

Gene position: 2141646-2140195 (Counterclockwise)

Preceding gene: 15674036

Following gene: 30024072

Centisome position: 90.53

GC content: 37.67

Gene sequence:

>1452_bases
ATGAATAAAAAAATCCGCGTCCGTTACGCACCATCACCAACAGGACTTTTGCATATCGGGAATGCTCGTACAGCCCTTTT
TAACTATCTTTTTGCCCGTCATCACGGGGGAGATTTTATCATCCGTATTGAAGATACAGACCGTGAACGTCACGTAGAAG
ATGGTGAGCGTTCACAACTTGAAAACCTTCGTTGGTTGGGAATGGATTGGGATGAAAGTCCTGAAACACATGAAAACTAC
CGTCAATCAGAACGTTTGCCTTTATATCAAAAATATATTGACCAATTGTTGACTGAAGGAAAAGCTTATTATTCTTATAA
AACGCCAGAAGAACTTGAAGCTGACCATGCGAAACAAGAAGCGGCAGGAATTGCTCCACACTATATTAACGAATATGCTG
GCATGAGTGATGATGAAAAAGCAGCTTATATCGCGGAGCGTAAAGCGCAAAATATTGAACCGGTTGTTCGTATTTCTGTT
GATGAAAAAGCAATTTATAAATGGAATGATATTGTTAAAGGCGACATTGAATTTGAAGGTAAAAACATCGGTGGAGATTG
GGTAATCCAAAAACGTGATGGTTACCCAACTTACAATTTTGCTGTTGTTGTTGATGACCATGATATGCAAATCTCACATG
TTATCCGTGGCGATGACCATATTGCCAATACACCAAAACAATTAGTTGTTTACGATGCTTTGGGTTGGGAAGCACCACAA
TTTGGTCACATGACTTTGATTATTAATTCTGAAACTGGGAAAAAATTATCAAAACGTGACACAAATACACTTCAGTTTAT
TGAAGACTACCGTAAAAAAGGTTACATGTCTGATGCCATTTTCAACTTTATTGCACTTTTGGGCTGGAATCCTGGTGGAG
AAAAAGAAATTTTCTCTCGTGAAGAATTAATTGAACTTTTTGATGAACATCGTTTGTCTAAATCACCAGCAGCCTTTGAC
CAAAAGAAATTGGACTGGTTGGATAATGAATACATCAAAAATGCTGATTTTGCTAAAGTATTTGAATTGACAAAACCATT
CCTTGTTGCTGCTAATCGCTTTGATGAACGTGCTGAGGAACTGGTTAAACTTTATCAACCACAAATGAAATCAGCAGATG
AAATTGTTGAATTAACAGACCTTTTCTATGGAGATTTCCCAGAATTGACTGATGAAGCTCGTGAAATGTTGGCTGCTGAA
ACAACACCATTAGCTTTGAGTACTTTCCGTGCAAAATTGGCTGAGCTTCCTGAAAATGATTTTACAGTGGAAAATATTTT
CCCACTCTTTAAAGCGACTCAAAAAGAAACTGGCGTAAAAGGTAAAATGCTTTGGATGCCAATTCGTATTGCAGCTTCAG
GTTCAATGCATGGTCCAGAATTACCAGAAACAATTGCACTTCTTGGAAAAGAAAAAGTATTGGCTCATTTGGACGCTGCT
TTAAATAAATAA

Upstream 100 bases:

>100_bases
TAGAGGTGATTGTTAGAGTAGTTTAGGCTATTTAAAACCAAGAACGATTCATTTCTTACAGAAAAGAAGTGAACGAATTA
ATGAGGAGAATTGGATTAAT

Downstream 100 bases:

>100_bases
TATAATAAAAAGACGCCGTGTGGCGTCTTTTTATTAAACCAAAATAGAACACTCAGACATTCAAATGGTATAAAATAAGG
AGATAAACTAACGAAAAGAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MNKKIRVRYAPSPTGLLHIGNARTALFNYLFARHHGGDFIIRIEDTDRERHVEDGERSQLENLRWLGMDWDESPETHENY
RQSERLPLYQKYIDQLLTEGKAYYSYKTPEELEADHAKQEAAGIAPHYINEYAGMSDDEKAAYIAERKAQNIEPVVRISV
DEKAIYKWNDIVKGDIEFEGKNIGGDWVIQKRDGYPTYNFAVVVDDHDMQISHVIRGDDHIANTPKQLVVYDALGWEAPQ
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMSDAIFNFIALLGWNPGGEKEIFSREELIELFDEHRLSKSPAAFD
QKKLDWLDNEYIKNADFAKVFELTKPFLVAANRFDERAEELVKLYQPQMKSADEIVELTDLFYGDFPELTDEAREMLAAE
TTPLALSTFRAKLAELPENDFTVENIFPLFKATQKETGVKGKMLWMPIRIAASGSMHGPELPETIALLGKEKVLAHLDAA
LNK

Sequences:

>Translated_483_residues
MNKKIRVRYAPSPTGLLHIGNARTALFNYLFARHHGGDFIIRIEDTDRERHVEDGERSQLENLRWLGMDWDESPETHENY
RQSERLPLYQKYIDQLLTEGKAYYSYKTPEELEADHAKQEAAGIAPHYINEYAGMSDDEKAAYIAERKAQNIEPVVRISV
DEKAIYKWNDIVKGDIEFEGKNIGGDWVIQKRDGYPTYNFAVVVDDHDMQISHVIRGDDHIANTPKQLVVYDALGWEAPQ
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMSDAIFNFIALLGWNPGGEKEIFSREELIELFDEHRLSKSPAAFD
QKKLDWLDNEYIKNADFAKVFELTKPFLVAANRFDERAEELVKLYQPQMKSADEIVELTDLFYGDFPELTDEAREMLAAE
TTPLALSTFRAKLAELPENDFTVENIFPLFKATQKETGVKGKMLWMPIRIAASGSMHGPELPETIALLGKEKVLAHLDAA
LNK
>Mature_483_residues
MNKKIRVRYAPSPTGLLHIGNARTALFNYLFARHHGGDFIIRIEDTDRERHVEDGERSQLENLRWLGMDWDESPETHENY
RQSERLPLYQKYIDQLLTEGKAYYSYKTPEELEADHAKQEAAGIAPHYINEYAGMSDDEKAAYIAERKAQNIEPVVRISV
DEKAIYKWNDIVKGDIEFEGKNIGGDWVIQKRDGYPTYNFAVVVDDHDMQISHVIRGDDHIANTPKQLVVYDALGWEAPQ
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMSDAIFNFIALLGWNPGGEKEIFSREELIELFDEHRLSKSPAAFD
QKKLDWLDNEYIKNADFAKVFELTKPFLVAANRFDERAEELVKLYQPQMKSADEIVELTDLFYGDFPELTDEAREMLAAE
TTPLALSTFRAKLAELPENDFTVENIFPLFKATQKETGVKGKMLWMPIRIAASGSMHGPELPETIALLGKEKVLAHLDAA
LNK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=323, Percent_Identity=37.7708978328173, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI62241042, Length=236, Percent_Identity=25, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788743, Length=483, Percent_Identity=38.0952380952381, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI226510925, Length=253, Percent_Identity=31.6205533596838, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71993394, Length=490, Percent_Identity=31.6326530612245, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17543332, Length=298, Percent_Identity=23.8255033557047, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=484, Percent_Identity=31.6115702479339, Blast_Score=227, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6321192, Length=217, Percent_Identity=26.2672811059908, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=312, Percent_Identity=24.0384615384615, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665321, Length=509, Percent_Identity=30.4518664047151, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI17864488, Length=300, Percent_Identity=25.6666666666667, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_LACLA (Q9CDZ7)

Other databases:

- EMBL:   AE005176
- PIR:   E86881
- RefSeq:   NP_268210.1
- ProteinModelPortal:   Q9CDZ7
- SMR:   Q9CDZ7
- GeneID:   1115730
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0349
- NMPDR:   fig|272623.1.peg.2108
- HOGENOM:   HBG628189
- OMA:   MANKIRV
- ProtClustDB:   PRK01406
- BioCyc:   LLAC272623:L0349-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 55462; Mature: 55462

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKIRVRYAPSPTGLLHIGNARTALFNYLFARHHGGDFIIRIEDTDRERHVEDGERSQL
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCHHCCCCCCHHHH
ENLRWLGMDWDESPETHENYRQSERLPLYQKYIDQLLTEGKAYYSYKTPEELEADHAKQE
HCCEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHH
AAGIAPHYINEYAGMSDDEKAAYIAERKAQNIEPVVRISVDEKAIYKWNDIVKGDIEFEG
HCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHEEECCCEEECEEEECC
KNIGGDWVIQKRDGYPTYNFAVVVDDHDMQISHVIRGDDHIANTPKQLVVYDALGWEAPQ
CCCCCCEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHCCCCCCEEEEECCCCCCCC
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMSDAIFNFIALLGWNPGGEKEIFSR
CCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHH
EELIELFDEHRLSKSPAAFDQKKLDWLDNEYIKNADFAKVFELTKPFLVAANRFDERAEE
HHHHHHHHHHCCCCCCCCCCHHHHHHHCHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHH
LVKLYQPQMKSADEIVELTDLFYGDFPELTDEAREMLAAETTPLALSTFRAKLAELPEND
HHHHHCHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
FTVENIFPLFKATQKETGVKGKMLWMPIRIAASGSMHGPELPETIALLGKEKVLAHLDAA
CCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
LNK
HCC
>Mature Secondary Structure
MNKKIRVRYAPSPTGLLHIGNARTALFNYLFARHHGGDFIIRIEDTDRERHVEDGERSQL
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCHHCCCCCCHHHH
ENLRWLGMDWDESPETHENYRQSERLPLYQKYIDQLLTEGKAYYSYKTPEELEADHAKQE
HCCEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHH
AAGIAPHYINEYAGMSDDEKAAYIAERKAQNIEPVVRISVDEKAIYKWNDIVKGDIEFEG
HCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHEEECCCEEECEEEECC
KNIGGDWVIQKRDGYPTYNFAVVVDDHDMQISHVIRGDDHIANTPKQLVVYDALGWEAPQ
CCCCCCEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHCCCCCCEEEEECCCCCCCC
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMSDAIFNFIALLGWNPGGEKEIFSR
CCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHH
EELIELFDEHRLSKSPAAFDQKKLDWLDNEYIKNADFAKVFELTKPFLVAANRFDERAEE
HHHHHHHHHHCCCCCCCCCCHHHHHHHCHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHH
LVKLYQPQMKSADEIVELTDLFYGDFPELTDEAREMLAAETTPLALSTFRAKLAELPEND
HHHHHCHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
FTVENIFPLFKATQKETGVKGKMLWMPIRIAASGSMHGPELPETIALLGKEKVLAHLDAA
CCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
LNK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471