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The map label for this gene is rcfA

Identifier: 15673985

GI number: 15673985

Start: 2082212

End: 2082880

Strand: Direct

Name: rcfA

Synonym: L0239

Alternate gene names: 15673985

Gene position: 2082212-2082880 (Clockwise)

Preceding gene: 15673984

Following gene: 15673986

Centisome position: 88.02

GC content: 34.08

Gene sequence:

>669_bases
ATGACTTTAGATAATCATCATTGCGTCCAATTAGTTCCACTCTTTGAAAAACTTGCTGAACAAGATTTAACTAAGGTCGA
AAAAATTGTTGAACACAAAAAAATTAAAAAAGGTGAAATTATTATTAGCCCTGACAAAGAACCTTTATTAGTCATTGTAG
CTCAAGGTGCACTTAAAATTTCACAACTTTCACAAAGTGGTAAAGAGCAACTTTTAAGAATCATCGAAGCAGGTGATTAT
GAAGGCGAAGGAGCCTTACTTGGCGTGACCAACGGACAACTTTATGGTCAGGCCATGATTGATTCTACGATTTGTTTCTT
GCGTCAATCTGATTTTGAGACATTATTAACGACTTATCCTAGTCTTTCATTACAACTTTTAAAACTTAATGCTAAAAAAT
ATCTAGCTGCTGAGCACCAAGCAGGATTTTTGGCACTAGATGATGTTGAAGCACGTCTTGCACATTACTTTTTGAATCTA
TCTGCCACTTCTGATAGCATTTATTTTGAAATTCCAATGAAACTCAAGGAGCTTGCCAATTATATCGGAACCAGTCCAGA
AACAATTTCACGTAAAATCAAGGTTTTTGAAGAAAATAAAATTATTAGCCGTCAAGGACGAATGATTAAACTTTTGGATA
AGGAGAAATTAGAAGATGATTTTGCTTAA

Upstream 100 bases:

>100_bases
AATACTATCCAAAAATTTCACTGTCGCAAATGACTAAAAATAAGGTTATAATTAAAGAAAATCTTTCAGGAAAAATATCT
ACTCAAATAAGGAGAATAAA

Downstream 100 bases:

>100_bases
AAATTTTACAAAAAAAAATAGAGTGAGTCAACTCTATTTTTTTTAGAAAATATTTCCAAATTACTTTAATTTAAGCTTCT
AACGCAAATTGTAAAATGAA

Product: transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MTLDNHHCVQLVPLFEKLAEQDLTKVEKIVEHKKIKKGEIIISPDKEPLLVIVAQGALKISQLSQSGKEQLLRIIEAGDY
EGEGALLGVTNGQLYGQAMIDSTICFLRQSDFETLLTTYPSLSLQLLKLNAKKYLAAEHQAGFLALDDVEARLAHYFLNL
SATSDSIYFEIPMKLKELANYIGTSPETISRKIKVFEENKIISRQGRMIKLLDKEKLEDDFA

Sequences:

>Translated_222_residues
MTLDNHHCVQLVPLFEKLAEQDLTKVEKIVEHKKIKKGEIIISPDKEPLLVIVAQGALKISQLSQSGKEQLLRIIEAGDY
EGEGALLGVTNGQLYGQAMIDSTICFLRQSDFETLLTTYPSLSLQLLKLNAKKYLAAEHQAGFLALDDVEARLAHYFLNL
SATSDSIYFEIPMKLKELANYIGTSPETISRKIKVFEENKIISRQGRMIKLLDKEKLEDDFA
>Mature_221_residues
TLDNHHCVQLVPLFEKLAEQDLTKVEKIVEHKKIKKGEIIISPDKEPLLVIVAQGALKISQLSQSGKEQLLRIIEAGDYE
GEGALLGVTNGQLYGQAMIDSTICFLRQSDFETLLTTYPSLSLQLLKLNAKKYLAAEHQAGFLALDDVEARLAHYFLNLS
ATSDSIYFEIPMKLKELANYIGTSPETISRKIKVFEENKIISRQGRMIKLLDKEKLEDDFA

Specific function: Global Transcription Factor That Controls The Expression Of Over 100 Target Genes In Response To Anoxia. It Facilitates The Adaptation To Anaerobic Growth Conditions By Regulating The Expression Of Gene Products That Are Involved In Anaerobic Energy Metab

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=207, Percent_Identity=26.0869565217391, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789756, Length=185, Percent_Identity=27.027027027027, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24980; Mature: 24849

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDNHHCVQLVPLFEKLAEQDLTKVEKIVEHKKIKKGEIIISPDKEPLLVIVAQGALKI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEECCCHHH
SQLSQSGKEQLLRIIEAGDYEGEGALLGVTNGQLYGQAMIDSTICFLRQSDFETLLTTYP
HHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHCCHHHHHHHCC
SLSLQLLKLNAKKYLAAEHQAGFLALDDVEARLAHYFLNLSATSDSIYFEIPMKLKELAN
CCEEEEEEECHHHHHHHHCCCCEEEEHHHHHHHHHHHHEEECCCCEEEEEECHHHHHHHH
YIGTSPETISRKIKVFEENKIISRQGRMIKLLDKEKLEDDFA
HHCCCHHHHHHHHHHHHCCCCHHCCCCEEEECCHHHHCCCCC
>Mature Secondary Structure 
TLDNHHCVQLVPLFEKLAEQDLTKVEKIVEHKKIKKGEIIISPDKEPLLVIVAQGALKI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEECCCHHH
SQLSQSGKEQLLRIIEAGDYEGEGALLGVTNGQLYGQAMIDSTICFLRQSDFETLLTTYP
HHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHCCHHHHHHHCC
SLSLQLLKLNAKKYLAAEHQAGFLALDDVEARLAHYFLNLSATSDSIYFEIPMKLKELAN
CCEEEEEEECHHHHHHHHCCCCEEEEHHHHHHHHHHHHEEECCCCEEEEEECHHHHHHHH
YIGTSPETISRKIKVFEENKIISRQGRMIKLLDKEKLEDDFA
HHCCCHHHHHHHHHHHHCCCCHHCCCCEEEECCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2113923; 8441692 [H]