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The map label for this gene is recF

Identifier: 15673957

GI number: 15673957

Start: 2051081

End: 2052157

Strand: Direct

Name: recF

Synonym: L0261

Alternate gene names: 15673957

Gene position: 2051081-2052157 (Clockwise)

Preceding gene: 15673956

Following gene: 15673959

Centisome position: 86.7

GC content: 33.05

Gene sequence:

>1077_bases
ATGAAACTCAAAGCAATTGAACTCAAAAATTTTCGTAATTATGAGGAATTAAAACTTGATTTTCACCCTAATCTCAATAT
TTTTTTAGGACAAAATGCTCAAGGGAAAACAAATATTCTTGAGGCGATTCATTTTTTAGCACTCACTCGTAGTCACAGAA
CAAGTCACGACAAAGAGCTCATTTCTTGGTCACAACAAGAAATGAAGGTCTCAGGAGTCGTTGAAAAAGCTCATGCAACT
GTTCCACTTGAAGTTCAATTAAGTCCCAAAGGGAGAATTGCTAAAGCCAATCATCTCAAAGAAAATCGCTTAGCCGACTA
CATTGGTCAACTAAAAATCTTGATGTTTGCACCTGAAAATTTGGAATTAGTCAAAGGTTCTCCAGCTACCCGCAGAAAAT
TTATGGATATTGAACTGGGACAAATCCATGCGGTTTACCTTTATGATTCCATGCGATATAATCGTGCGTTAAAAGAACGG
AATGCTTACTTAAAATTTGATAAAGACAAAATTGATAAAAATTTTTTAAGTGTTCTTGACGGACAATTAGCTGAACATGG
TAATAAAATCATGCTTGAGCGGCAAAATTTTATTGATAAACTTGAAGTTCATGCAAAAAAAATCCATGAGCAACTTACTC
ATGGTAAAGAAAATTTAAAAATCATTTATAATCAAAATGTGAAAACTGACTTTTCAAAAGAATTATTGAGCAGACAAGAC
CATGATATTTTTAGGCATCAAACATCGGTTGGCCCTCATCGTGACGATTTACAATTTTTTATCAATGAGATTAATGTTGC
TGATTTTGGTAGTCAAGGACAACAACGAACCGTTGCTCTCTCAATTAAATTAGCAGAAATTGATTTAATTTTTGAAGAGA
CTGGTGAGTATCCGATTTTACTTTTAGATGATGTCATGAGTGAATTAGACAATCATCGTCAACTTGATTTGATTGAAACA
AGTTTGGGTAAAACACAAACTTTTATAACTACAACTACTCTTGATCATCTTAAAAATTTACCTGAAAATCTGAGTATTTT
CCATGTTAATGCTGGAACCATTGAACAAGAGCAATAA

Upstream 100 bases:

>100_bases
AAGAAGCGCGTGTTAAAGCTATTGTGAAAAAAATGAACGCGGAAAACAAGACGACAAAACCAGCTAAAAAAGCTCCGCCA
CGTTTTCCTGGTAGAAAATA

Downstream 100 bases:

>100_bases
AAAGTTACTGATGAAACTACTAGCATATCTGTAAACTGCTAAAGCAGTAACAGCTATCCTATGACAAAAATTCATCAGTA
ACTTTTTTACTTTCTGTCAG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKLKAIELKNFRNYEELKLDFHPNLNIFLGQNAQGKTNILEAIHFLALTRSHRTSHDKELISWSQQEMKVSGVVEKAHAT
VPLEVQLSPKGRIAKANHLKENRLADYIGQLKILMFAPENLELVKGSPATRRKFMDIELGQIHAVYLYDSMRYNRALKER
NAYLKFDKDKIDKNFLSVLDGQLAEHGNKIMLERQNFIDKLEVHAKKIHEQLTHGKENLKIIYNQNVKTDFSKELLSRQD
HDIFRHQTSVGPHRDDLQFFINEINVADFGSQGQQRTVALSIKLAEIDLIFEETGEYPILLLDDVMSELDNHRQLDLIET
SLGKTQTFITTTTLDHLKNLPENLSIFHVNAGTIEQEQ

Sequences:

>Translated_358_residues
MKLKAIELKNFRNYEELKLDFHPNLNIFLGQNAQGKTNILEAIHFLALTRSHRTSHDKELISWSQQEMKVSGVVEKAHAT
VPLEVQLSPKGRIAKANHLKENRLADYIGQLKILMFAPENLELVKGSPATRRKFMDIELGQIHAVYLYDSMRYNRALKER
NAYLKFDKDKIDKNFLSVLDGQLAEHGNKIMLERQNFIDKLEVHAKKIHEQLTHGKENLKIIYNQNVKTDFSKELLSRQD
HDIFRHQTSVGPHRDDLQFFINEINVADFGSQGQQRTVALSIKLAEIDLIFEETGEYPILLLDDVMSELDNHRQLDLIET
SLGKTQTFITTTTLDHLKNLPENLSIFHVNAGTIEQEQ
>Mature_358_residues
MKLKAIELKNFRNYEELKLDFHPNLNIFLGQNAQGKTNILEAIHFLALTRSHRTSHDKELISWSQQEMKVSGVVEKAHAT
VPLEVQLSPKGRIAKANHLKENRLADYIGQLKILMFAPENLELVKGSPATRRKFMDIELGQIHAVYLYDSMRYNRALKER
NAYLKFDKDKIDKNFLSVLDGQLAEHGNKIMLERQNFIDKLEVHAKKIHEQLTHGKENLKIIYNQNVKTDFSKELLSRQD
HDIFRHQTSVGPHRDDLQFFINEINVADFGSQGQQRTVALSIKLAEIDLIFEETGEYPILLLDDVMSELDNHRQLDLIET
SLGKTQTFITTTTLDHLKNLPENLSIFHVNAGTIEQEQ

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=359, Percent_Identity=24.2339832869081, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 41357; Mature: 41357

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKAIELKNFRNYEELKLDFHPNLNIFLGQNAQGKTNILEAIHFLALTRSHRTSHDKEL
CCEEEEECCCCCCHHHEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
ISWSQQEMKVSGVVEKAHATVPLEVQLSPKGRIAKANHLKENRLADYIGQLKILMFAPEN
HHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEHHHCCHHHHHHHHHCCEEEEEECCCC
LELVKGSPATRRKFMDIELGQIHAVYLYDSMRYNRALKERNAYLKFDKDKIDKNFLSVLD
CEEEECCCHHHHEEEEEEECCEEEEEEECCHHHHHHHHHCCCEEEECHHHHHHHHHHHHC
GQLAEHGNKIMLERQNFIDKLEVHAKKIHEQLTHGKENLKIIYNQNVKTDFSKELLSRQD
CHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCC
HDIFRHQTSVGPHRDDLQFFINEINVADFGSQGQQRTVALSIKLAEIDLIFEETGEYPIL
HHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEEEEECCCCCCEE
LLDDVMSELDNHRQLDLIETSLGKTQTFITTTTLDHLKNLPENLSIFHVNAGTIEQEQ
EHHHHHHHHCCCCEEEHHHHCCCCCEEEEEEEHHHHHHCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MKLKAIELKNFRNYEELKLDFHPNLNIFLGQNAQGKTNILEAIHFLALTRSHRTSHDKEL
CCEEEEECCCCCCHHHEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
ISWSQQEMKVSGVVEKAHATVPLEVQLSPKGRIAKANHLKENRLADYIGQLKILMFAPEN
HHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEHHHCCHHHHHHHHHCCEEEEEECCCC
LELVKGSPATRRKFMDIELGQIHAVYLYDSMRYNRALKERNAYLKFDKDKIDKNFLSVLD
CEEEECCCHHHHEEEEEEECCEEEEEEECCHHHHHHHHHCCCEEEECHHHHHHHHHHHHC
GQLAEHGNKIMLERQNFIDKLEVHAKKIHEQLTHGKENLKIIYNQNVKTDFSKELLSRQD
CHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCC
HDIFRHQTSVGPHRDDLQFFINEINVADFGSQGQQRTVALSIKLAEIDLIFEETGEYPIL
HHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEEEEECCCCCCEE
LLDDVMSELDNHRQLDLIETSLGKTQTFITTTTLDHLKNLPENLSIFHVNAGTIEQEQ
EHHHHHHHHCCCCEEEHHHHCCCCCEEEEEEEHHHHHHCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA