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The map label for this gene is hisS

Identifier: 15673949

GI number: 15673949

Start: 2042397

End: 2043689

Strand: Reverse

Name: hisS

Synonym: L0342

Alternate gene names: 15673949

Gene position: 2043689-2042397 (Counterclockwise)

Preceding gene: 15673950

Following gene: 15673948

Centisome position: 86.39

GC content: 37.12

Gene sequence:

>1293_bases
ATGAAACTTCAAAAACCAAAAGGAACAGCAGATTTACTGCCTGCTGAAACTGCAAAATGGCAGTACATCGAAGAAATTGC
TCGTGGCGTTTTCAATGATTATAATTTCAAAGAAATTCGGACACCAATGTTTGAAAGCTATGAACTTTTTAGTCGTGCAA
CAGGTGAAACAAGTGATATTGTTACCAAAGAAATGTATGATTTTGAGGATAAAGGGGGACGTCATATTGCGCTTCGTCCA
GAAGGAACTGCCTCAGCTGTTCGGGCTTATATTGAGAATAAACTCTATGCCCCTGAAGTAGTAAAACCAGTGAAGCTATG
GTATGACGCACCAATGTTTCGTTATGAACGTCCACAATCAGGACGTTTACGTCAATTTCATCAATTTGGCGTAGAGTGTT
TAGGAGTGAAAAATCCAGCAGTTGATGTAGAAATTATTGCAATGGCTGACACACTTTTCCGCCAGTTAGGAATTACTGGT
CTTAAACTAGCTCTGAATACACTTGGAGATATGGAAAGTCGCATGGCTTACCGTCAAGCATTGATTGATTATTTGACTCC
TTTTGAGAATCAACTTTCAGAAGATAGCCGTCGTCGTCTCAATGAAAATCCTTTACGCGTTTTGGATAGTAAAGAAGCAG
AAGACATCGCAATTGTTAAAAATGCTCCAGCTATTTTAGATTATCTCAATGAAGCTTCAAAGGCTTATTTTGAAGAAGTG
AAAGCCTTACTTGAAGCTTTACATATTGAGTACACTATTGACCCAAATATGGTTCGTGGTTTGGATTATTATAATGATAC
AATTTTTGAATTTATTGTTGATTTTGATGGCAAAGATTTGACCGTATGTGGTGGTGGACGTTATGATGGGTTAGTTGAAT
ATTTCGACGGACCAGCAACTCCTGCCTTTGGATTTGGTTTAGGAATTGAAAGACTTTTGATGATTGCTCAAAAACAAGAA
ATTAATTTCATTCCTGAAGAAACTTTGGATGTTTATATTGCTGTAATGGGCGAAAAAGCAAATCTTGAAGCAACAAAATT
AGCTGAAAGCCTACGTGAACAGGCTTTTAAAGTCGAACGTGATTTCTCAAATCGAAAACTAGGAGCGCAATTTAAAACGG
CTGAAAAATTGGGTGCTGAGTTGATTATTACACTTGGAGAAGATGAGATTCGCACAGGTCAAATCAAAGTGAAACATAAT
CAAACACGTAAACAAGTAGAAACAACACTTAAAGCTGTTCATGAATCATTTGCACCGATTTTTGAAGAGATTTATGCGGA
TGAAGAACTTTAA

Upstream 100 bases:

>100_bases
TTAGGGTGCTTTCTTATGTAAACGGTATCTTTGAAATGAATGTTCAAACCCAATGAATAATCATAAAGATTTTTACTCCC
AGTTAACCGAAAGGAAAAAA

Downstream 100 bases:

>100_bases
GTGACTTAAAGCAGGGGTGAATTGATGACGGATTGTCCATTTTGTCAGATGAAAGATACAATCTTTGAAAATGAATTGGC
ACAGGCATTTTATGATGCCT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKLQKPKGTADLLPAETAKWQYIEEIARGVFNDYNFKEIRTPMFESYELFSRATGETSDIVTKEMYDFEDKGGRHIALRP
EGTASAVRAYIENKLYAPEVVKPVKLWYDAPMFRYERPQSGRLRQFHQFGVECLGVKNPAVDVEIIAMADTLFRQLGITG
LKLALNTLGDMESRMAYRQALIDYLTPFENQLSEDSRRRLNENPLRVLDSKEAEDIAIVKNAPAILDYLNEASKAYFEEV
KALLEALHIEYTIDPNMVRGLDYYNDTIFEFIVDFDGKDLTVCGGGRYDGLVEYFDGPATPAFGFGLGIERLLMIAQKQE
INFIPEETLDVYIAVMGEKANLEATKLAESLREQAFKVERDFSNRKLGAQFKTAEKLGAELIITLGEDEIRTGQIKVKHN
QTRKQVETTLKAVHESFAPIFEEIYADEEL

Sequences:

>Translated_430_residues
MKLQKPKGTADLLPAETAKWQYIEEIARGVFNDYNFKEIRTPMFESYELFSRATGETSDIVTKEMYDFEDKGGRHIALRP
EGTASAVRAYIENKLYAPEVVKPVKLWYDAPMFRYERPQSGRLRQFHQFGVECLGVKNPAVDVEIIAMADTLFRQLGITG
LKLALNTLGDMESRMAYRQALIDYLTPFENQLSEDSRRRLNENPLRVLDSKEAEDIAIVKNAPAILDYLNEASKAYFEEV
KALLEALHIEYTIDPNMVRGLDYYNDTIFEFIVDFDGKDLTVCGGGRYDGLVEYFDGPATPAFGFGLGIERLLMIAQKQE
INFIPEETLDVYIAVMGEKANLEATKLAESLREQAFKVERDFSNRKLGAQFKTAEKLGAELIITLGEDEIRTGQIKVKHN
QTRKQVETTLKAVHESFAPIFEEIYADEEL
>Mature_430_residues
MKLQKPKGTADLLPAETAKWQYIEEIARGVFNDYNFKEIRTPMFESYELFSRATGETSDIVTKEMYDFEDKGGRHIALRP
EGTASAVRAYIENKLYAPEVVKPVKLWYDAPMFRYERPQSGRLRQFHQFGVECLGVKNPAVDVEIIAMADTLFRQLGITG
LKLALNTLGDMESRMAYRQALIDYLTPFENQLSEDSRRRLNENPLRVLDSKEAEDIAIVKNAPAILDYLNEASKAYFEEV
KALLEALHIEYTIDPNMVRGLDYYNDTIFEFIVDFDGKDLTVCGGGRYDGLVEYFDGPATPAFGFGLGIERLLMIAQKQE
INFIPEETLDVYIAVMGEKANLEATKLAESLREQAFKVERDFSNRKLGAQFKTAEKLGAELIITLGEDEIRTGQIKVKHN
QTRKQVETTLKAVHESFAPIFEEIYADEEL

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=455, Percent_Identity=26.3736263736264, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI6996014, Length=461, Percent_Identity=26.0303687635575, Blast_Score=106, Evalue=5e-23,
Organism=Escherichia coli, GI1788861, Length=416, Percent_Identity=43.9903846153846, Blast_Score=354, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI71993686, Length=470, Percent_Identity=27.2340425531915, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71993693, Length=470, Percent_Identity=27.2340425531915, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325290, Length=473, Percent_Identity=24.1014799154334, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24643061, Length=471, Percent_Identity=24.6284501061571, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24643059, Length=491, Percent_Identity=24.0325865580448, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_LACLA (Q9CE78)

Other databases:

- EMBL:   AE005176
- PIR:   G86870
- RefSeq:   NP_268124.1
- ProteinModelPortal:   Q9CE78
- SMR:   Q9CE78
- PhosSite:   Q9CE78
- GeneID:   1115641
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0342
- NMPDR:   fig|272623.1.peg.2020
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   LLAC272623:L0342-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 49004; Mature: 49004

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQKPKGTADLLPAETAKWQYIEEIARGVFNDYNFKEIRTPMFESYELFSRATGETSDI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCHHHH
VTKEMYDFEDKGGRHIALRPEGTASAVRAYIENKLYAPEVVKPVKLWYDAPMFRYERPQS
HHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHCCCCCC
GRLRQFHQFGVECLGVKNPAVDVEIIAMADTLFRQLGITGLKLALNTLGDMESRMAYRQA
CHHHHHHHCCCEEEECCCCCCCEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LIDYLTPFENQLSEDSRRRLNENPLRVLDSKEAEDIAIVKNAPAILDYLNEASKAYFEEV
HHHHHCCHHHHHCHHHHHHCCCCCHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
KALLEALHIEYTIDPNMVRGLDYYNDTIFEFIVDFDGKDLTVCGGGRYDGLVEYFDGPAT
HHHHHHHHEEEEECCHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCC
PAFGFGLGIERLLMIAQKQEINFIPEETLDVYIAVMGEKANLEATKLAESLREQAFKVER
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
DFSNRKLGAQFKTAEKLGAELIITLGEDEIRTGQIKVKHNQTRKQVETTLKAVHESFAPI
CCCCCCCCCHHHHHHHCCCEEEEEECCCCCEECEEEEECCHHHHHHHHHHHHHHHHHHHH
FEEIYADEEL
HHHHHCCCCC
>Mature Secondary Structure
MKLQKPKGTADLLPAETAKWQYIEEIARGVFNDYNFKEIRTPMFESYELFSRATGETSDI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCHHHH
VTKEMYDFEDKGGRHIALRPEGTASAVRAYIENKLYAPEVVKPVKLWYDAPMFRYERPQS
HHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHCCCCCC
GRLRQFHQFGVECLGVKNPAVDVEIIAMADTLFRQLGITGLKLALNTLGDMESRMAYRQA
CHHHHHHHCCCEEEECCCCCCCEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LIDYLTPFENQLSEDSRRRLNENPLRVLDSKEAEDIAIVKNAPAILDYLNEASKAYFEEV
HHHHHCCHHHHHCHHHHHHCCCCCHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
KALLEALHIEYTIDPNMVRGLDYYNDTIFEFIVDFDGKDLTVCGGGRYDGLVEYFDGPAT
HHHHHHHHEEEEECCHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCC
PAFGFGLGIERLLMIAQKQEINFIPEETLDVYIAVMGEKANLEATKLAESLREQAFKVER
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
DFSNRKLGAQFKTAEKLGAELIITLGEDEIRTGQIKVKHNQTRKQVETTLKAVHESFAPI
CCCCCCCCCHHHHHHHCCCEEEEEECCCCCEECEEEEECCHHHHHHHHHHHHHHHHHHHH
FEEIYADEEL
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471