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The map label for this gene is ytjE

Identifier: 15673898

GI number: 15673898

Start: 1994162

End: 1995325

Strand: Reverse

Name: ytjE

Synonym: L177593

Alternate gene names: 15673898

Gene position: 1995325-1994162 (Counterclockwise)

Preceding gene: 15673899

Following gene: 15673897

Centisome position: 84.35

GC content: 33.08

Gene sequence:

>1164_bases
ATGACAAAATATGATTTTACAACCATTCCAAATCGACTTGACCACAATTCTGTAAAATGGCAAGAAATAAAAGAGAATCC
TCATAAACTTCCATTATGGGTGGCTGATATGGATTTTCTTGCCTTACCAGAAATAAAACAAAGTATTCATGATTACGCAG
ACTATGGAGTTTATGGATATGCCTATGTAGAAGAAGAATTAATTAAAAGCATTCAAAATTGGGAAAAAAATCAACATCAG
TATGACTTTTCTAAAGAAGCTTTAATTTTGGTTGAAGGAGTTGTTCCAGGAATTGGTCTGGCCATTCAAGCATTGACCAA
AGAAAATGATGCTGTTTTAATAAACACACCTGTTTATCCCCCGTTTGCCAGAACAGTTAAGCTAAATAATCGAAAGCTAA
TTGAAAATAAACTGATGGAAAAAAATTGGGAATTTCTAATTGATTTTGAAGTTTTTGAAAAAGAAATTGTTGAAAATTCC
GTGAAATTATATATTCTTTGTAACCCCCACAATCCTGGTGGGCGCGTGTGGACGAAAGAAGAACTGATAAAACTTGGCCA
AATTTGTAAAAAGCATAATGTGATTGTCGTTTCTGATGAAATTCATCAAGATTTAACATTGTTTGGTCATCAGCACCAGA
CCTTTAACACTCTTTCAGAAGACTTCAAAGATTTTTCAGTTGTTTTATCTTCTGCCACCAAAAGTTTTAACATTGCTGGC
GTGAAATGCGCTTATGCAATTATTGAAAACCAAGATTTACGTGAAAAATACTTGCAAAGAAGACTAGCAAATAATCAACA
AGAAATTAGCACTTTAGGAATGCTAGCCACCAAAGTTGCTTATGAAAATGGAAGTGAATGGTTAAATGAACTCAAAAATG
TGCTTGAAAAAAATATCACTTATATTGAGTCAGAACTTCATCAAAAAACAAAAATCAAAGTAATGAAACCTCAAGGAACC
TATTTAATTTGGCTTGATTTTTCTGAATATCAATTAGAAGATAAGGTATTGGATGAAAAATTAGCAGAAGAGGCAGGTGT
GATTCTAAATAAAGGAATAAGCTTTGGAAAAGCTGGACAAGCGCATGCCAGATTAAATGCTGCTGCACCTTTTTCATTGA
CTGAAGAAGCAAGCAAACGACTCGTAGAAACATTCAAAATTTAA

Upstream 100 bases:

>100_bases
CTGTCAGTAAATTTTTTACTGATGGAGCTACTATCATATCTGTAAACTGCTAAAGCAGTGACAGCTATCCTATGACGAAA
AGAAAAAAGAGAGTATAAAA

Downstream 100 bases:

>100_bases
GGATTTTAGTCGAAAATGTGTTTTTAAGAATGAAAAATATGTTAAAATCAACTTATGAATGATATTTTACAACTCACAAT
TGTTTTAATTGCCTCTTTAA

Product: aminotransferase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MTKYDFTTIPNRLDHNSVKWQEIKENPHKLPLWVADMDFLALPEIKQSIHDYADYGVYGYAYVEEELIKSIQNWEKNQHQ
YDFSKEALILVEGVVPGIGLAIQALTKENDAVLINTPVYPPFARTVKLNNRKLIENKLMEKNWEFLIDFEVFEKEIVENS
VKLYILCNPHNPGGRVWTKEELIKLGQICKKHNVIVVSDEIHQDLTLFGHQHQTFNTLSEDFKDFSVVLSSATKSFNIAG
VKCAYAIIENQDLREKYLQRRLANNQQEISTLGMLATKVAYENGSEWLNELKNVLEKNITYIESELHQKTKIKVMKPQGT
YLIWLDFSEYQLEDKVLDEKLAEEAGVILNKGISFGKAGQAHARLNAAAPFSLTEEASKRLVETFKI

Sequences:

>Translated_387_residues
MTKYDFTTIPNRLDHNSVKWQEIKENPHKLPLWVADMDFLALPEIKQSIHDYADYGVYGYAYVEEELIKSIQNWEKNQHQ
YDFSKEALILVEGVVPGIGLAIQALTKENDAVLINTPVYPPFARTVKLNNRKLIENKLMEKNWEFLIDFEVFEKEIVENS
VKLYILCNPHNPGGRVWTKEELIKLGQICKKHNVIVVSDEIHQDLTLFGHQHQTFNTLSEDFKDFSVVLSSATKSFNIAG
VKCAYAIIENQDLREKYLQRRLANNQQEISTLGMLATKVAYENGSEWLNELKNVLEKNITYIESELHQKTKIKVMKPQGT
YLIWLDFSEYQLEDKVLDEKLAEEAGVILNKGISFGKAGQAHARLNAAAPFSLTEEASKRLVETFKI
>Mature_386_residues
TKYDFTTIPNRLDHNSVKWQEIKENPHKLPLWVADMDFLALPEIKQSIHDYADYGVYGYAYVEEELIKSIQNWEKNQHQY
DFSKEALILVEGVVPGIGLAIQALTKENDAVLINTPVYPPFARTVKLNNRKLIENKLMEKNWEFLIDFEVFEKEIVENSV
KLYILCNPHNPGGRVWTKEELIKLGQICKKHNVIVVSDEIHQDLTLFGHQHQTFNTLSEDFKDFSVVLSSATKSFNIAGV
KCAYAIIENQDLREKYLQRRLANNQQEISTLGMLATKVAYENGSEWLNELKNVLEKNITYIESELHQKTKIKVMKPQGTY
LIWLDFSEYQLEDKVLDEKLAEEAGVILNKGISFGKAGQAHARLNAAAPFSLTEEASKRLVETFKI

Specific function: Catalyzes the transformation of cystathionine to homocysteine. Also exhibits cysteine desulfhydrase activity in vitro, producing sulfide from cysteine [H]

COG id: COG1168

COG function: function code E; Bifunctional PLP-dependent enzyme with beta-cystathionase and maltose regulon repressor activities

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/PatB cystathionine beta-lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI56713256, Length=266, Percent_Identity=26.6917293233083, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI56713254, Length=288, Percent_Identity=26.3888888888889, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI95147551, Length=249, Percent_Identity=24.8995983935743, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI169881279, Length=249, Percent_Identity=24.8995983935743, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI169881281, Length=180, Percent_Identity=24.4444444444444, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787909, Length=389, Percent_Identity=28.7917737789203, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1788627, Length=314, Percent_Identity=24.203821656051, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786816, Length=283, Percent_Identity=22.6148409893993, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788722, Length=305, Percent_Identity=22.2950819672131, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17567663, Length=306, Percent_Identity=22.8758169934641, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71994476, Length=181, Percent_Identity=30.3867403314917, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71994472, Length=181, Percent_Identity=30.3867403314917, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322401, Length=314, Percent_Identity=27.7070063694268, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI18859735, Length=399, Percent_Identity=23.5588972431078, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28573069, Length=169, Percent_Identity=30.1775147928994, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646114, Length=169, Percent_Identity=30.1775147928994, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28573067, Length=169, Percent_Identity=30.1775147928994, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28573065, Length=169, Percent_Identity=30.1775147928994, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 44552; Mature: 44421

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYDFTTIPNRLDHNSVKWQEIKENPHKLPLWVADMDFLALPEIKQSIHDYADYGVYGY
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCHHCCHHHHHHHHHHHHCCCEEH
AYVEEELIKSIQNWEKNQHQYDFSKEALILVEGVVPGIGLAIQALTKENDAVLINTPVYP
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHEEECCCCEEEEECCCCC
PFARTVKLNNRKLIENKLMEKNWEFLIDFEVFEKEIVENSVKLYILCNPHNPGGRVWTKE
CCHHEEEECCCHHHHHHHHHCCCCEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHH
ELIKLGQICKKHNVIVVSDEIHQDLTLFGHQHQTFNTLSEDFKDFSVVLSSATKSFNIAG
HHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VKCAYAIIENQDLREKYLQRRLANNQQEISTLGMLATKVAYENGSEWLNELKNVLEKNIT
CEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
YIESELHQKTKIKVMKPQGTYLIWLDFSEYQLEDKVLDEKLAEEAGVILNKGISFGKAGQ
HHHHHHCCCCEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCC
AHARLNAAAPFSLTEEASKRLVETFKI
CHHEECCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKYDFTTIPNRLDHNSVKWQEIKENPHKLPLWVADMDFLALPEIKQSIHDYADYGVYGY
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCCHHCCHHHHHHHHHHHHCCCEEH
AYVEEELIKSIQNWEKNQHQYDFSKEALILVEGVVPGIGLAIQALTKENDAVLINTPVYP
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHEEECCCCEEEEECCCCC
PFARTVKLNNRKLIENKLMEKNWEFLIDFEVFEKEIVENSVKLYILCNPHNPGGRVWTKE
CCHHEEEECCCHHHHHHHHHCCCCEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHH
ELIKLGQICKKHNVIVVSDEIHQDLTLFGHQHQTFNTLSEDFKDFSVVLSSATKSFNIAG
HHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VKCAYAIIENQDLREKYLQRRLANNQQEISTLGMLATKVAYENGSEWLNELKNVLEKNIT
CEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
YIESELHQKTKIKVMKPQGTYLIWLDFSEYQLEDKVLDEKLAEEAGVILNKGISFGKAGQ
HHHHHHCCCCEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCC
AHARLNAAAPFSLTEEASKRLVETFKI
CHHEECCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497199; 9274030; 9384377 [H]