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The map label for this gene is malE

Identifier: 15673665

GI number: 15673665

Start: 1739050

End: 1740282

Strand: Direct

Name: malE

Synonym: L128695

Alternate gene names: 15673665

Gene position: 1739050-1740282 (Clockwise)

Preceding gene: 15673645

Following gene: 15673666

Centisome position: 73.51

GC content: 39.42

Gene sequence:

>1233_bases
ATGAAATCATGGAAAAAAGTTGCTCTTGGTGGAGCATCTGTACTCGCCCTCGCAACCCTCGCAGCTTGCGGTTCATCAGC
AAGTTCTAACAAATCATCATCTTCTTCTAGCTCAAACAATGTTAAAGGTAATGTTACCTTGTGGGTTGACCCAGCTAATG
TTGATAGCTACAAAACACTAGTAACTGGCTTTGAAAAAAAATATCCTGATGTTAAAGTAAAAGTCAATCAATCTCCAACA
GGTTCTGCAAATGCCAAAACTGACGTTGGTAAAGACCCAGCAAAAGCAGCGGATGTCTTCAAAGTTCCTAATGACCAACT
TGGTGCCATGGCTGAAGCAGGTTACATTAACCCTCTTTCTCCTGATGCAACAAAATGGGTAGACAAAAATGACATTTCTG
TTGCTGGAGAAGCTGTTTCTTGGAAAGGTAAATATTATGCCTATCCTCAAGACCAACAATCAAATATCATCTTCTATAAC
AAAGCTAAATTCTCTGAAGCACCAACTAGTTGGACTCAATTTACACAAGATAAAGCAATCGGTACAGACTTTACAAACTC
TTATAACTGGTATCCAGCCTTCCTTTCAAACGGTACAGTATTGTTTGGTAAAGATGGTGAAACTTTAGACGGTACTGACG
TTGCCGGAGATGCTGGACTTCAAGTCTTGAATTGGTTTGCAAAACAAAAATCTAACACAGGTGTTGTCCAATCAGGTCAA
GCATTGCTAGCTGATTTAAAATCAGGTAAAACAGCTGCTGTCCTCGATGGACCTTGGGATGCAAGTAATGTTAAGAAAAT
TCTTGGCGACAATTACGGTGTGACCACCCTTCCAACAATTGATTTCGGCTCTGGCGAAAAGAAAATGCAAGCTTTCTCTG
GTGTAGGTACACTTGCTGTTAACTCTGCTTCTAAAAATCAAGTCGCTGCATCTTCTCTCGCTCAATATCTTTCTAATGCT
GACTCACAAAAAGAACTTTATAAAGATAATAATGCAATCCCAGTTGCTAAAAGTTTGCAAACTGATTCAGATATTACCGC
AGACCCAGCTGCCCAAGCTGTTATCAAACAAGTGCCTGAAGATACTTTGATGCCTAAAATGCCTGAAATGGCCACATTCT
GGAATTTAGCTGCTCCTTTGATTAATAATACATACCTTGGTAAGACTCCTGCTTCACAATATGACAGTCAACTTAAAACT
TTCCAAGATTCTATCTCTAAAGCAACTAAATAA

Upstream 100 bases:

>100_bases
GTAATCAAAATGTAATATTTTTTCCCTTGACAAAAAGCAAACGGTTGTGTAAAACGAGGAGTGTATAAAATAAACCGTTT
TCATTAGGAGGAAACACAAA

Downstream 100 bases:

>100_bases
TTCATTAGTACTCTTTTAAAGAGGTTGGGCACAGCCTAATCTCTTTATTTATTAGAAAAGGACAAAGTTATATGTTCAGA
AAAAAAACTTCTTTACCACC

Product: maltose ABC transporter substrate binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKSWKKVALGGASVLALATLAACGSSASSNKSSSSSSSNNVKGNVTLWVDPANVDSYKTLVTGFEKKYPDVKVKVNQSPT
GSANAKTDVGKDPAKAADVFKVPNDQLGAMAEAGYINPLSPDATKWVDKNDISVAGEAVSWKGKYYAYPQDQQSNIIFYN
KAKFSEAPTSWTQFTQDKAIGTDFTNSYNWYPAFLSNGTVLFGKDGETLDGTDVAGDAGLQVLNWFAKQKSNTGVVQSGQ
ALLADLKSGKTAAVLDGPWDASNVKKILGDNYGVTTLPTIDFGSGEKKMQAFSGVGTLAVNSASKNQVAASSLAQYLSNA
DSQKELYKDNNAIPVAKSLQTDSDITADPAAQAVIKQVPEDTLMPKMPEMATFWNLAAPLINNTYLGKTPASQYDSQLKT
FQDSISKATK

Sequences:

>Translated_410_residues
MKSWKKVALGGASVLALATLAACGSSASSNKSSSSSSSNNVKGNVTLWVDPANVDSYKTLVTGFEKKYPDVKVKVNQSPT
GSANAKTDVGKDPAKAADVFKVPNDQLGAMAEAGYINPLSPDATKWVDKNDISVAGEAVSWKGKYYAYPQDQQSNIIFYN
KAKFSEAPTSWTQFTQDKAIGTDFTNSYNWYPAFLSNGTVLFGKDGETLDGTDVAGDAGLQVLNWFAKQKSNTGVVQSGQ
ALLADLKSGKTAAVLDGPWDASNVKKILGDNYGVTTLPTIDFGSGEKKMQAFSGVGTLAVNSASKNQVAASSLAQYLSNA
DSQKELYKDNNAIPVAKSLQTDSDITADPAAQAVIKQVPEDTLMPKMPEMATFWNLAAPLINNTYLGKTPASQYDSQLKT
FQDSISKATK
>Mature_410_residues
MKSWKKVALGGASVLALATLAACGSSASSNKSSSSSSSNNVKGNVTLWVDPANVDSYKTLVTGFEKKYPDVKVKVNQSPT
GSANAKTDVGKDPAKAADVFKVPNDQLGAMAEAGYINPLSPDATKWVDKNDISVAGEAVSWKGKYYAYPQDQQSNIIFYN
KAKFSEAPTSWTQFTQDKAIGTDFTNSYNWYPAFLSNGTVLFGKDGETLDGTDVAGDAGLQVLNWFAKQKSNTGVVQSGQ
ALLADLKSGKTAAVLDGPWDASNVKKILGDNYGVTTLPTIDFGSGEKKMQAFSGVGTLAVNSASKNQVAASSLAQYLSNA
DSQKELYKDNNAIPVAKSLQTDSDITADPAAQAVIKQVPEDTLMPKMPEMATFWNLAAPLINNTYLGKTPASQYDSQLKT
FQDSISKATK

Specific function: Binding protein for cyclodextrin; involved in its cellular uptake. Interacts with all natural cyclodextrins:alpha, beta and gamma [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=414, Percent_Identity=25.8454106280193, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 43552; Mature: 43552

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSWKKVALGGASVLALATLAACGSSASSNKSSSSSSSNNVKGNVTLWVDPANVDSYKTL
CCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHH
VTGFEKKYPDVKVKVNQSPTGSANAKTDVGKDPAKAADVFKVPNDQLGAMAEAGYINPLS
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHEEECCCHHHHHHHHCCCCCCCC
PDATKWVDKNDISVAGEAVSWKGKYYAYPQDQQSNIIFYNKAKFSEAPTSWTQFTQDKAI
CCHHHCCCCCCCEECCCEEEECCCEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCC
GTDFTNSYNWYPAFLSNGTVLFGKDGETLDGTDVAGDAGLQVLNWFAKQKSNTGVVQSGQ
CCCCCCCCCCEEHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCH
ALLADLKSGKTAAVLDGPWDASNVKKILGDNYGVTTLPTIDFGSGEKKMQAFSGVGTLAV
HHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCCEEECEEECCCCHHHHHHHHCCCEEEE
NSASKNQVAASSLAQYLSNADSQKELYKDNNAIPVAKSLQTDSDITADPAAQAVIKQVPE
CCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHCCCCCCCCCCCHHHHHHHHHCCC
DTLMPKMPEMATFWNLAAPLINNTYLGKTPASQYDSQLKTFQDSISKATK
HHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSWKKVALGGASVLALATLAACGSSASSNKSSSSSSSNNVKGNVTLWVDPANVDSYKTL
CCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHH
VTGFEKKYPDVKVKVNQSPTGSANAKTDVGKDPAKAADVFKVPNDQLGAMAEAGYINPLS
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHEEECCCHHHHHHHHCCCCCCCC
PDATKWVDKNDISVAGEAVSWKGKYYAYPQDQQSNIIFYNKAKFSEAPTSWTQFTQDKAI
CCHHHCCCCCCCEECCCEEEECCCEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCC
GTDFTNSYNWYPAFLSNGTVLFGKDGETLDGTDVAGDAGLQVLNWFAKQKSNTGVVQSGQ
CCCCCCCCCCEEHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCH
ALLADLKSGKTAAVLDGPWDASNVKKILGDNYGVTTLPTIDFGSGEKKMQAFSGVGTLAV
HHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCCEEECEEECCCCHHHHHHHHCCCEEEE
NSASKNQVAASSLAQYLSNADSQKELYKDNNAIPVAKSLQTDSDITADPAAQAVIKQVPE
CCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHCCCCCCCCCCCHHHHHHHHHCCC
DTLMPKMPEMATFWNLAAPLINNTYLGKTPASQYDSQLKTFQDSISKATK
HHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]