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The map label for this gene is hisH

Identifier: 15673194

GI number: 15673194

Start: 1234887

End: 1235495

Strand: Direct

Name: hisH

Synonym: L0069

Alternate gene names: 15673194

Gene position: 1234887-1235495 (Clockwise)

Preceding gene: 15673193

Following gene: 15673195

Centisome position: 52.2

GC content: 36.29

Gene sequence:

>609_bases
ATGAAAAAAATTGTTATCATCGACTACAATATTGGAAATCTTCAAAGTGTACAGGCCGCATTTTTACGATTGGGACAAGA
AACAGTGATTTCAAGAGATTTAGAGGAAATTCGTAAAGCAGATGCACTTATTCTTCCAGGAGTTGGGGCTTTTCCTACAG
CAATGAATAATCTAAAAAAGTTTAATTTGATTGAACTTATACAAGAACGAGCTGCTGCTGGAATACCAATATTGGGAATT
TGTTTAGGAATGCAAGTGCTTTTTGAAAAAGGATACGAGATAGAAGAAAGACAAGGTCTTGGACTTTTAAAGGGAGAGGT
GATTCCAATCAAAACTAATGAGAAAATTCCGCATATGGGATGGAATCAATTAAACCTGGCTAAAGCCAGTCCAACAACTC
ATTATTTGTCTGATAATGATGAGGTCTATTTTGTCCATTCTTATCAGGCGACTTGTCCTGATGATGAACTCATTGCCTAC
ACCACTTATGGCGAAGTAAAAATTCCGGCAATTGTTGGAAAAAATAATGTGATAGGCTGTCAATTTCACCCTGAAAAGAG
TGGAGAAATTGGCAGAAAGGTACTAAAAGCATTCTTGGAGGAAATTTAA

Upstream 100 bases:

>100_bases
GTCGGGATAGAATCGATGTTGATCGTTTGAAATTGGTTGGCTGCTGTGAGGTTTTTGGTTAAATTGAATTTTAGTATATT
TTGATATTGAAAGGAGTATT

Downstream 100 bases:

>100_bases
ATGAAGATTATTCCAGCAATTGATTTGCAAAATGGTGAAGCCGTGCGTCTCTACAAAGGAGATTATGATAAGAAAACGGT
CTATTCAAAAACTCCCCTTG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MKKIVIIDYNIGNLQSVQAAFLRLGQETVISRDLEEIRKADALILPGVGAFPTAMNNLKKFNLIELIQERAAAGIPILGI
CLGMQVLFEKGYEIEERQGLGLLKGEVIPIKTNEKIPHMGWNQLNLAKASPTTHYLSDNDEVYFVHSYQATCPDDELIAY
TTYGEVKIPAIVGKNNVIGCQFHPEKSGEIGRKVLKAFLEEI

Sequences:

>Translated_202_residues
MKKIVIIDYNIGNLQSVQAAFLRLGQETVISRDLEEIRKADALILPGVGAFPTAMNNLKKFNLIELIQERAAAGIPILGI
CLGMQVLFEKGYEIEERQGLGLLKGEVIPIKTNEKIPHMGWNQLNLAKASPTTHYLSDNDEVYFVHSYQATCPDDELIAY
TTYGEVKIPAIVGKNNVIGCQFHPEKSGEIGRKVLKAFLEEI
>Mature_202_residues
MKKIVIIDYNIGNLQSVQAAFLRLGQETVISRDLEEIRKADALILPGVGAFPTAMNNLKKFNLIELIQERAAAGIPILGI
CLGMQVLFEKGYEIEERQGLGLLKGEVIPIKTNEKIPHMGWNQLNLAKASPTTHYLSDNDEVYFVHSYQATCPDDELIAY
TTYGEVKIPAIVGKNNVIGCQFHPEKSGEIGRKVLKAFLEEI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=201, Percent_Identity=42.2885572139304, Blast_Score=130, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6319725, Length=213, Percent_Identity=33.8028169014084, Blast_Score=104, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_LACLA (Q02132)

Other databases:

- EMBL:   U92974
- EMBL:   AE005176
- PIR:   D86776
- PIR:   I45734
- RefSeq:   NP_267368.1
- ProteinModelPortal:   Q02132
- SMR:   Q02132
- GeneID:   1114860
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0069
- NMPDR:   fig|272623.1.peg.1247
- HOGENOM:   HBG292341
- OMA:   HVEKSQT
- ProtClustDB:   PRK13141
- BioCyc:   LLAC272623:L0069-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22454; Mature: 22454

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 81-81 ACT_SITE 183-183 ACT_SITE 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVIIDYNIGNLQSVQAAFLRLGQETVISRDLEEIRKADALILPGVGAFPTAMNNLKK
CCEEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHH
FNLIELIQERAAAGIPILGICLGMQVLFEKGYEIEERQGLGLLKGEVIPIKTNEKIPHMG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHCCCCEECCCEEEEECCCCCCCCC
WNQLNLAKASPTTHYLSDNDEVYFVHSYQATCPDDELIAYTTYGEVKIPAIVGKNNVIGC
CCCEEEECCCCCEEEECCCCCEEEEEECCCCCCCCCEEEEEECCCEEEEEEECCCCEEEE
QFHPEKSGEIGRKVLKAFLEEI
EECCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MKKIVIIDYNIGNLQSVQAAFLRLGQETVISRDLEEIRKADALILPGVGAFPTAMNNLKK
CCEEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHH
FNLIELIQERAAAGIPILGICLGMQVLFEKGYEIEERQGLGLLKGEVIPIKTNEKIPHMG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHCCCCEECCCEEEEECCCCCCCCC
WNQLNLAKASPTTHYLSDNDEVYFVHSYQATCPDDELIAYTTYGEVKIPAIVGKNNVIGC
CCCEEEECCCCCEEEECCCCCEEEEEECCCCCCCCCEEEEEECCCEEEEEEECCCCEEEE
QFHPEKSGEIGRKVLKAFLEEI
EECCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400209; 11337471