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The map label for this gene is folC

Identifier: 15673139

GI number: 15673139

Start: 1168825

End: 1170108

Strand: Direct

Name: folC

Synonym: L0177

Alternate gene names: 15673139

Gene position: 1168825-1170108 (Clockwise)

Preceding gene: 15673138

Following gene: 15673140

Centisome position: 49.41

GC content: 36.37

Gene sequence:

>1284_bases
ATGTCTATAGAAGAAGCATTGGAATGGATACATTCACGTTTAAAATTTAATATTCGCCCAGGCCTAAGTCGTGTTTCGGC
CCTTTTAGAATTGCTTGGTCATCCAGAAGAGTCTTTGTCAATGATTCACGTTGCTGGAACAAATGGAAAAGGCTCCACAG
TCGCTTTCACACGCTCAATCTTTATGCAGGCAGGTCTGAAGGTTGCTTCTTTCACAAGTCCTTTCATCACCACTTTTGGT
GAGCGGATGTCGATTAATGCACTCCCGATTGCTGATGATAAATTAATTTATTATGTAGAAATGATCCAACCACTTGTTGC
TGAACTTGATAAAGATGCTGAACTGACTGGAATTACCGAATTTGAAATTATCACGGCAATGGCTTTTAAATATTTCTCTG
ATGAGCAGGTTGATTTAGCGGTTATTGAAGTTGGTTTAGGTGGACTTCTTGATTCAACAAATGTGATTAAACCTGTTGTT
TCTGGAATTACAACAATTGGTTTAGATCATATTGATATTCTTGGTTCGACCATTGAAGAAATCGCAGCTCAAAAGGCTGG
AATTATTAAACCAGGAATTCCAGTAGTTGTTGGAAATATTGAATTAAAAGCACTTCGGGTTATATGGGAAGTGGCTAGAA
AAAATACAGCGCGTGTTTATCAATTTCCATATGATTATCGTACGGAAGTGGAAGAACACGAACATTTTAATTTCTTTTCT
GGTCAAGAAGCAATATTGGATATTGAAAAATCTTTAGTTGGCTTACATCAAATAGAAAATGCTGGTATGGCTATTGAACT
TTCTCTGGTTTATGCAAGTAAGGTTGGGATTGAATTGACTGAGGATGTGATTCGCTCTGGAATTCGTGAGGCTTTTTGGC
CAGCTCGTATGGAAAAATTGGGTGAAAAACCACTCATTTTACTGGATGGTGCTCATAATGTTCATGCGATGAATCGTTTG
CTTGAAAATCTTAGCTCTGAGTTTCCAGATAAAAAAATTACAATCATTTTTTCAGCCATTACCACAAAAGATATTAGTCA
AATGATAAAAATGCTTCAAACTGTGAAAAATTCGCATCTGATTTTGACAACTTTTGATTATCCAAAAGCTTTGAATTTGG
GAGATTTTCAAAGATTGGAAGAAGAAGGGGTTGAATTGGCTCCAAGTTGGGAATTAGCTTTAGTTCGTGCGCAAAAAAAT
TTAGCTGAAGATGATTTGTTATTAGTTACAGGCTCTCTCTATTTCTCATCTCAAGTTCGTGAGTTTTTGAAAAAAGAGAA
GTAA

Upstream 100 bases:

>100_bases
CCTTGCTGAATTGCCTTTTTTCCTTCTTGATAGAACTGAAGCTCCTGATGAATTTAAAAAATATTATCAATACAAAAGAT
AAAGTAGAAAAGGTCACAAA

Downstream 100 bases:

>100_bases
TAACTAAAATGATTTTTTAGTTTTTAAAATTAATAAATAGTTGTAAATTTAAAAAATTCGGCTATAATAAAATAGATTTA
TAAATTCATTTCTTTCTTTT

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSIEEALEWIHSRLKFNIRPGLSRVSALLELLGHPEESLSMIHVAGTNGKGSTVAFTRSIFMQAGLKVASFTSPFITTFG
ERMSINALPIADDKLIYYVEMIQPLVAELDKDAELTGITEFEIITAMAFKYFSDEQVDLAVIEVGLGGLLDSTNVIKPVV
SGITTIGLDHIDILGSTIEEIAAQKAGIIKPGIPVVVGNIELKALRVIWEVARKNTARVYQFPYDYRTEVEEHEHFNFFS
GQEAILDIEKSLVGLHQIENAGMAIELSLVYASKVGIELTEDVIRSGIREAFWPARMEKLGEKPLILLDGAHNVHAMNRL
LENLSSEFPDKKITIIFSAITTKDISQMIKMLQTVKNSHLILTTFDYPKALNLGDFQRLEEEGVELAPSWELALVRAQKN
LAEDDLLLVTGSLYFSSQVREFLKKEK

Sequences:

>Translated_427_residues
MSIEEALEWIHSRLKFNIRPGLSRVSALLELLGHPEESLSMIHVAGTNGKGSTVAFTRSIFMQAGLKVASFTSPFITTFG
ERMSINALPIADDKLIYYVEMIQPLVAELDKDAELTGITEFEIITAMAFKYFSDEQVDLAVIEVGLGGLLDSTNVIKPVV
SGITTIGLDHIDILGSTIEEIAAQKAGIIKPGIPVVVGNIELKALRVIWEVARKNTARVYQFPYDYRTEVEEHEHFNFFS
GQEAILDIEKSLVGLHQIENAGMAIELSLVYASKVGIELTEDVIRSGIREAFWPARMEKLGEKPLILLDGAHNVHAMNRL
LENLSSEFPDKKITIIFSAITTKDISQMIKMLQTVKNSHLILTTFDYPKALNLGDFQRLEEEGVELAPSWELALVRAQKN
LAEDDLLLVTGSLYFSSQVREFLKKEK
>Mature_426_residues
SIEEALEWIHSRLKFNIRPGLSRVSALLELLGHPEESLSMIHVAGTNGKGSTVAFTRSIFMQAGLKVASFTSPFITTFGE
RMSINALPIADDKLIYYVEMIQPLVAELDKDAELTGITEFEIITAMAFKYFSDEQVDLAVIEVGLGGLLDSTNVIKPVVS
GITTIGLDHIDILGSTIEEIAAQKAGIIKPGIPVVVGNIELKALRVIWEVARKNTARVYQFPYDYRTEVEEHEHFNFFSG
QEAILDIEKSLVGLHQIENAGMAIELSLVYASKVGIELTEDVIRSGIREAFWPARMEKLGEKPLILLDGAHNVHAMNRLL
ENLSSEFPDKKITIIFSAITTKDISQMIKMLQTVKNSHLILTTFDYPKALNLGDFQRLEEEGVELAPSWELALVRAQKNL
AEDDLLLVTGSLYFSSQVREFLKKEK

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=364, Percent_Identity=31.8681318681319, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI66932984, Length=364, Percent_Identity=31.8681318681319, Blast_Score=167, Evalue=1e-41,
Organism=Escherichia coli, GI1788654, Length=430, Percent_Identity=28.6046511627907, Blast_Score=155, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17553148, Length=347, Percent_Identity=28.5302593659942, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI71984923, Length=347, Percent_Identity=28.5302593659942, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17553150, Length=347, Percent_Identity=28.5302593659942, Blast_Score=137, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6323760, Length=361, Percent_Identity=32.409972299169, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6324815, Length=302, Percent_Identity=31.1258278145695, Blast_Score=126, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6322718, Length=368, Percent_Identity=25.8152173913043, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24641571, Length=291, Percent_Identity=33.3333333333333, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24581568, Length=194, Percent_Identity=29.8969072164948, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47578; Mature: 47446

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00589 PTS_HPR_SER ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEEALEWIHSRLKFNIRPGLSRVSALLELLGHPEESLSMIHVAGTNGKGSTVAFTRSI
CCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCHHCEEEEEECCCCCCCCHHHHHHH
FMQAGLKVASFTSPFITTFGERMSINALPIADDKLIYYVEMIQPLVAELDKDAELTGITE
HHHHCCCHHHCCCHHHHHCCCCEECCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHH
FEIITAMAFKYFSDEQVDLAVIEVGLGGLLDSTNVIKPVVSGITTIGLDHIDILGSTIEE
HHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
IAAQKAGIIKPGIPVVVGNIELKALRVIWEVARKNTARVYQFPYDYRTEVEEHEHFNFFS
HHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCEEEECCCCHHCCCHHCCCCCCCC
GQEAILDIEKSLVGLHQIENAGMAIELSLVYASKVGIELTEDVIRSGIREAFWPARMEKL
CCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHCCCHHHHHHHHHHHHHHHCHHHHHHC
GEKPLILLDGAHNVHAMNRLLENLSSEFPDKKITIIFSAITTKDISQMIKMLQTVKNSHL
CCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCEE
ILTTFDYPKALNLGDFQRLEEEGVELAPSWELALVRAQKNLAEDDLLLVTGSLYFSSQVR
EEEECCCCCCCCCHHHHHHHHCCCEECCCHHHEEEEHHHCCCCCCEEEEECHHHHHHHHH
EFLKKEK
HHHHHCC
>Mature Secondary Structure 
SIEEALEWIHSRLKFNIRPGLSRVSALLELLGHPEESLSMIHVAGTNGKGSTVAFTRSI
CHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCHHCEEEEEECCCCCCCCHHHHHHH
FMQAGLKVASFTSPFITTFGERMSINALPIADDKLIYYVEMIQPLVAELDKDAELTGITE
HHHHCCCHHHCCCHHHHHCCCCEECCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHH
FEIITAMAFKYFSDEQVDLAVIEVGLGGLLDSTNVIKPVVSGITTIGLDHIDILGSTIEE
HHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
IAAQKAGIIKPGIPVVVGNIELKALRVIWEVARKNTARVYQFPYDYRTEVEEHEHFNFFS
HHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCEEEECCCCHHCCCHHCCCCCCCC
GQEAILDIEKSLVGLHQIENAGMAIELSLVYASKVGIELTEDVIRSGIREAFWPARMEKL
CCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHCCCHHHHHHHHHHHHHHHCHHHHHHC
GEKPLILLDGAHNVHAMNRLLENLSSEFPDKKITIIFSAITTKDISQMIKMLQTVKNSHL
CCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCEE
ILTTFDYPKALNLGDFQRLEEEGVELAPSWELALVRAQKNLAEDDLLLVTGSLYFSSQVR
EEEECCCCCCCCCHHHHHHHHCCCEECCCHHHEEEEHHHCCCCCCEEEEECHHHHHHHHH
EFLKKEK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]