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The map label for this gene is dfrA

Identifier: 15673132

GI number: 15673132

Start: 1163134

End: 1163640

Strand: Direct

Name: dfrA

Synonym: L162872

Alternate gene names: 15673132

Gene position: 1163134-1163640 (Clockwise)

Preceding gene: 15673129

Following gene: 30024025

Centisome position: 49.17

GC content: 33.53

Gene sequence:

>507_bases
ATGATAATTGGAATATGGGCAGAAGATGAAGCAGGTCTTATCGGTGAAGCTGATAAAATGCCTTGGTCTTTACCTGCTGA
ACAACAACATTTTAAAGAAACAACCATGAATCAAGTGATTTTGATGGGACGAAAAACGTTTGAAGGCATGAATAAACGTG
TATTGCCAGGGAGAATAAGTATTATTTTAACTCGCGATGAAACTTATCAATCAGATAATGAAAAAGTGCTCATCATGCAC
AGCCCTAAGGAAGTTCTAGATTGGTACCATAAGCAAAATAAAGACTTATTTATCACAGGAGGAGCTGAAATTTTAGCCCT
TTTTGAATCTGAACTTGAATTGCTCTATCGAACAGTTGTTCATGAAAAATTTAAAGGAGATACTTATTTTCCAAGTACAT
TTGACTTTGGAAGATTTAAGCTAGTCTCTGAAAAATTTCACGATAAAGATGAGCGGAATTCTTATACTTTTACAATTAAA
AAATATGAAAAAGTGAAACAACCATGA

Upstream 100 bases:

>100_bases
ATTATTTTTAAAAATGAGTGACTAATATCAAACTTGTCAAAGCGAAAACAGCAAAAATTTGCTATAATAGTAAAAGTAAA
AATCAATGAGAGGGGTTGCT

Downstream 100 bases:

>100_bases
CAAAATCAATTTTTGGGCTTTTCACAGCTCTCCTTTGTTGGATTAGCATTGTCATCGCTATTCAATGCTTTAGAAAAAAA
CGTTGGGGTCTGGGAGTATT

Product: dihydropholate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MIIGIWAEDEAGLIGEADKMPWSLPAEQQHFKETTMNQVILMGRKTFEGMNKRVLPGRISIILTRDETYQSDNEKVLIMH
SPKEVLDWYHKQNKDLFITGGAEILALFESELELLYRTVVHEKFKGDTYFPSTFDFGRFKLVSEKFHDKDERNSYTFTIK
KYEKVKQP

Sequences:

>Translated_168_residues
MIIGIWAEDEAGLIGEADKMPWSLPAEQQHFKETTMNQVILMGRKTFEGMNKRVLPGRISIILTRDETYQSDNEKVLIMH
SPKEVLDWYHKQNKDLFITGGAEILALFESELELLYRTVVHEKFKGDTYFPSTFDFGRFKLVSEKFHDKDERNSYTFTIK
KYEKVKQP
>Mature_168_residues
MIIGIWAEDEAGLIGEADKMPWSLPAEQQHFKETTMNQVILMGRKTFEGMNKRVLPGRISIILTRDETYQSDNEKVLIMH
SPKEVLDWYHKQNKDLFITGGAEILALFESELELLYRTVVHEKFKGDTYFPSTFDFGRFKLVSEKFHDKDERNSYTFTIK
KYEKVKQP

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain

Homologues:

Organism=Escherichia coli, GI1786233, Length=152, Percent_Identity=30.9210526315789, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): DYR_LACLA (Q59487)

Other databases:

- EMBL:   X60681
- EMBL:   AE005176
- PIR:   F86768
- PIR:   S47467
- RefSeq:   NP_267306.1
- ProteinModelPortal:   Q59487
- SMR:   Q59487
- GeneID:   1114791
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L162872
- NMPDR:   fig|272623.1.peg.1183
- HOGENOM:   HBG665708
- OMA:   QDEEGVI
- ProtClustDB:   CLSK797157
- BioCyc:   LLAC272623:L162872-MONOMER
- BRENDA:   1.5.1.3
- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796
- PANTHER:   PTHR11549:SF1
- PRINTS:   PR00070

Pfam domain/function: PF00186 DHFR_1

EC number: =1.5.1.3

Molecular weight: Translated: 19729; Mature: 19729

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00075 DHFR_1; PS51330 DHFR_2

Important sites: BINDING 27-27 BINDING 58-58 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGIWAEDEAGLIGEADKMPWSLPAEQQHFKETTMNQVILMGRKTFEGMNKRVLPGRIS
CEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEECCHHHHCCCCEECCCEEE
IILTRDETYQSDNEKVLIMHSPKEVLDWYHKQNKDLFITGGAEILALFESELELLYRTVV
EEEECCCCCCCCCCEEEEEECCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHH
HEKFKGDTYFPSTFDFGRFKLVSEKFHDKDERNSYTFTIKKYEKVKQP
HHHHCCCCCCCCCCCCCCEEEHHHHHCCCCCCCCEEEEEEEHHHCCCC
>Mature Secondary Structure
MIIGIWAEDEAGLIGEADKMPWSLPAEQQHFKETTMNQVILMGRKTFEGMNKRVLPGRIS
CEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEECCHHHHCCCCEECCCEEE
IILTRDETYQSDNEKVLIMHSPKEVLDWYHKQNKDLFITGGAEILALFESELELLYRTVV
EEEECCCCCCCCCCEEEEEECCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHH
HEKFKGDTYFPSTFDFGRFKLVSEKFHDKDERNSYTFTIKKYEKVKQP
HHHHCCCCCCCCCCCCCCEEEHHHHHCCCCCCCCEEEEEEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7574597; 11337471