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The map label for this gene is guaC

Identifier: 15673127

GI number: 15673127

Start: 1158457

End: 1159446

Strand: Direct

Name: guaC

Synonym: L158186

Alternate gene names: 15673127

Gene position: 1158457-1159446 (Clockwise)

Preceding gene: 15673126

Following gene: 15673128

Centisome position: 48.97

GC content: 38.59

Gene sequence:

>990_bases
ATGAATAATCTAAACTCAGTATTTGACTACGAAGACATCCAACTTATCCCAAATAAATGTGTCATTAACAGCCGTTTAGA
AGCAGACACAAGCGTAAAACTAGGAAATTTTACGTTCAAGCTCCCTGTTGTTCCAGCGAATATGCAAACCATTATTGATG
ATAAAATTGCTGAAATGCTTGCAAAAGAAGGCTATTTCTATATCATGCACCGTTTTGAAGCAGAAAATAGAGCGGCATTT
ATAAAAAAAATGCACCAACAAGGCCTGATTGCTTCAATTTCTGTTGGTGTTAAAGCAGATGAACATGCTTTTATTCGTGA
AATTTCAGCAGACGCACTCATTCCCGAATTTATTACAATTGATATTGCTCATGGACATGCAGATTCTGTTATTAAAACGA
TTCAATTGATTAAACGTTTGATGCCACAAACTTTTGTGATTGCTGGAAATGTTGGAACGCCAGAAGCTGTCCGTGAATTG
GAAAACGCTGGAGCAGATGCGACTAAAGTAGGCATTGGTCCAGGGAAAGTATGTATCACAAAAGTTAAAACTGGATTTGG
TACAGGTGGTTGGCAACTTGCAGCGGTAAAATGGTGTGCTAAAGCAGCAAGTAAACCAGTTATTGCTGACGGCGGAATTC
GTACCCATGGCGATATTGCGAAATCAATCCGTATGGGAGCAACAATGGTAATGGTCGGTTCACTTTTTGCTGCACACGAA
GAATCACCAGGACAAACTGTTGAACGTGACGGACAACTTTTCAAAGAATATTTTGGTTCGGCTTCAGAATATCAAAAAGG
TGAACACAAAAATGTCGAAGGAAAGAAAATTCTCCTTCCTCACAAAGGCTCACTTAAAGATACATTGAAAGAAATGGAAG
AAGATCTCCAAAGTTCAATTTCTTATGCTGGTGGTCGTGATTTGTCTGCGCTGACAAAAGTTGATTATGTTGTTGTTAAA
AACTCAATTTGGAATGGTGATGCGATTTAG

Upstream 100 bases:

>100_bases
ACCCGAAGGGTTTGGTCGGTCTTTTCTGTAAATCAAATCATAAATGAGTTCACAGGCTAGAAATTCATAAAAGCCTACTC
ATTCTCAAGGAGAAATTGCA

Downstream 100 bases:

>100_bases
TAAGCATTTTCTTTCCCATTGTTCGGGAATACCAAAACTTATGCTTGAAAAACTTTAGCATTTAGTCTATAATAGAACAA
TCTTATTTATACCTAGGATA

Product: guanosine 5'-monophosphate oxidoreductase

Products: NA

Alternate protein names: Guanosine 5'-monophosphate oxidoreductase; Guanosine monophosphate reductase

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNNLNSVFDYEDIQLIPNKCVINSRLEADTSVKLGNFTFKLPVVPANMQTIIDDKIAEMLAKEGYFYIMHRFEAENRAAF
IKKMHQQGLIASISVGVKADEHAFIREISADALIPEFITIDIAHGHADSVIKTIQLIKRLMPQTFVIAGNVGTPEAVREL
ENAGADATKVGIGPGKVCITKVKTGFGTGGWQLAAVKWCAKAASKPVIADGGIRTHGDIAKSIRMGATMVMVGSLFAAHE
ESPGQTVERDGQLFKEYFGSASEYQKGEHKNVEGKKILLPHKGSLKDTLKEMEEDLQSSISYAGGRDLSALTKVDYVVVK
NSIWNGDAI

Sequences:

>Translated_329_residues
MNNLNSVFDYEDIQLIPNKCVINSRLEADTSVKLGNFTFKLPVVPANMQTIIDDKIAEMLAKEGYFYIMHRFEAENRAAF
IKKMHQQGLIASISVGVKADEHAFIREISADALIPEFITIDIAHGHADSVIKTIQLIKRLMPQTFVIAGNVGTPEAVREL
ENAGADATKVGIGPGKVCITKVKTGFGTGGWQLAAVKWCAKAASKPVIADGGIRTHGDIAKSIRMGATMVMVGSLFAAHE
ESPGQTVERDGQLFKEYFGSASEYQKGEHKNVEGKKILLPHKGSLKDTLKEMEEDLQSSISYAGGRDLSALTKVDYVVVK
NSIWNGDAI
>Mature_329_residues
MNNLNSVFDYEDIQLIPNKCVINSRLEADTSVKLGNFTFKLPVVPANMQTIIDDKIAEMLAKEGYFYIMHRFEAENRAAF
IKKMHQQGLIASISVGVKADEHAFIREISADALIPEFITIDIAHGHADSVIKTIQLIKRLMPQTFVIAGNVGTPEAVREL
ENAGADATKVGIGPGKVCITKVKTGFGTGGWQLAAVKWCAKAASKPVIADGGIRTHGDIAKSIRMGATMVMVGSLFAAHE
ESPGQTVERDGQLFKEYFGSASEYQKGEHKNVEGKKILLPHKGSLKDTLKEMEEDLQSSISYAGGRDLSALTKVDYVVVK
NSIWNGDAI

Specific function: Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides

COG id: COG0516

COG function: function code F; IMP dehydrogenase/GMP reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI156104880, Length=326, Percent_Identity=30.6748466257669, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI50541954, Length=335, Percent_Identity=33.134328358209, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI50541952, Length=335, Percent_Identity=33.134328358209, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI50541948, Length=335, Percent_Identity=33.134328358209, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI50541956, Length=335, Percent_Identity=33.134328358209, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI217035148, Length=249, Percent_Identity=30.9236947791165, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI217035150, Length=251, Percent_Identity=31.4741035856574, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI217035146, Length=251, Percent_Identity=31.4741035856574, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI34328930, Length=251, Percent_Identity=31.4741035856574, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI217035152, Length=251, Percent_Identity=31.4741035856574, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI156616279, Length=249, Percent_Identity=30.9236947791165, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI34328928, Length=251, Percent_Identity=31.4741035856574, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI66933016, Length=150, Percent_Identity=40, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1786293, Length=341, Percent_Identity=32.5513196480938, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1788855, Length=237, Percent_Identity=36.2869198312236, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17560440, Length=323, Percent_Identity=31.2693498452012, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI71994389, Length=151, Percent_Identity=37.7483443708609, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71994385, Length=151, Percent_Identity=37.7483443708609, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323585, Length=231, Percent_Identity=29.8701298701299, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322012, Length=249, Percent_Identity=29.3172690763052, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323464, Length=217, Percent_Identity=31.3364055299539, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24641071, Length=259, Percent_Identity=30.1158301158301, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24641073, Length=259, Percent_Identity=30.1158301158301, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28571163, Length=259, Percent_Identity=30.1158301158301, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): GUAC_LACLA (Q9CGF1)

Other databases:

- EMBL:   AE005176
- PIR:   A86768
- RefSeq:   NP_267301.1
- ProteinModelPortal:   Q9CGF1
- SMR:   Q9CGF1
- GeneID:   1114786
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L158186
- NMPDR:   fig|272623.1.peg.1178
- HOGENOM:   HBG298985
- OMA:   PDYITID
- ProtClustDB:   PRK05458
- BioCyc:   LLAC272623:L158186-MONOMER
- BRENDA:   1.7.1.7
- HAMAP:   MF_01511
- InterPro:   IPR013785
- InterPro:   IPR005994
- InterPro:   IPR015875
- InterPro:   IPR001093
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF036500
- TIGRFAMs:   TIGR01306

Pfam domain/function: PF00478 IMPDH

EC number: =1.7.1.7

Molecular weight: Translated: 35838; Mature: 35838

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00487 IMP_DH_GMP_RED

Important sites: ACT_SITE 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLNSVFDYEDIQLIPNKCVINSRLEADTSVKLGNFTFKLPVVPANMQTIIDDKIAEML
CCCCCCCCCCCCCEECCCCEEECCCCCCCCEEEECCEEEEEEEECCCHHHHHHHHHHHHH
AKEGYFYIMHRFEAENRAAFIKKMHQQGLIASISVGVKADEHAFIREISADALIPEFITI
HHCCEEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEE
DIAHGHADSVIKTIQLIKRLMPQTFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCIT
EEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCEEEE
KVKTGFGTGGWQLAAVKWCAKAASKPVIADGGIRTHGDIAKSIRMGATMVMVGSLFAAHE
EECCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHCC
ESPGQTVERDGQLFKEYFGSASEYQKGEHKNVEGKKILLPHKGSLKDTLKEMEEDLQSSI
CCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHH
SYAGGRDLSALTKVDYVVVKNSIWNGDAI
HHCCCCCHHHHHHHEEEEEECCCCCCCCC
>Mature Secondary Structure
MNNLNSVFDYEDIQLIPNKCVINSRLEADTSVKLGNFTFKLPVVPANMQTIIDDKIAEML
CCCCCCCCCCCCCEECCCCEEECCCCCCCCEEEECCEEEEEEEECCCHHHHHHHHHHHHH
AKEGYFYIMHRFEAENRAAFIKKMHQQGLIASISVGVKADEHAFIREISADALIPEFITI
HHCCEEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEE
DIAHGHADSVIKTIQLIKRLMPQTFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCIT
EEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCEEEE
KVKTGFGTGGWQLAAVKWCAKAASKPVIADGGIRTHGDIAKSIRMGATMVMVGSLFAAHE
EECCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHCC
ESPGQTVERDGQLFKEYFGSASEYQKGEHKNVEGKKILLPHKGSLKDTLKEMEEDLQSSI
CCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHH
SYAGGRDLSALTKVDYVVVKNSIWNGDAI
HHCCCCCHHHHHHHEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471