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The map label for this gene is truB

Identifier: 15673108

GI number: 15673108

Start: 1140387

End: 1141376

Strand: Direct

Name: truB

Synonym: L0328

Alternate gene names: 15673108

Gene position: 1140387-1141376 (Clockwise)

Preceding gene: 15673107

Following gene: 15673109

Centisome position: 48.21

GC content: 37.68

Gene sequence:

>990_bases
ATGAATGAAAATTTAAATGGCATTTTAAATGTCTATAAAGAAGCGGGCTGGACATCTTTTGATGTTGTAGCCAAACTTCG
TGGTATTTTAAAAACAAAAAAAATTGGACATGGTGGAACTTTGGACCCTTCTGTGACAGGCGTTTTGCCAGTGGCCGTTG
GAAAATCGACGCGGTTATTAGAATACATGGAAGCGGCAGGTAAAATTTACGAAGGTGAAGTTACTATTGGTTTTTCAACG
GAAACCGAAGATGCTGACGGGGAAATTGTTAGTAAAACGCCAGTAGAACGGGAGCTGACAGAGTCTGAAATTGACTCAGT
AATGAAAAATTTTATTGGAAAAATAAAGCAAATTCCTCCAATGTATTCAGCCGTAAAAATTAACGGAAAAAAATTGTATG
AATATGCCAGAGCTGGTCAGACAGTTGACCGTCCGACAAGAGAGATTACAATCAAATCATTTGTCAGAACAAGTCCCATG
ATGACTGATGAAGCGGCAGGTTTGGTAAGCTTTTCTTTCAAAGTTGAGTGCAGTAAAGGAACCTATGTCCGAACATTAGC
AGTAGATTTAGCAGAGCAACTTGGTTATGCAGGACATATGTCAAAATTGCAAAGAACAGCAAGTAATGGACTGATGATTA
AAGATGCTGTCAAACTCTCAGAGATTGAAGTAGCAATGGAGGCAGGAGAGCTTTCTAGCTTAATGTACCCAGCAGAATAT
GCTGTCAGTGATTTGCCAAGAGTAAATTTGACTGCTGATCAGTTTGAAATGGCACGAGTTGGAAAAAAATTTGCTGAGGA
TGATTGGGTTACAGATGAAACTACTGACAGCCCTGTCATTAAGTCTAATAAAAGTAAATTTTTACTGACAGACTTGTCAG
AACTGACAAGAGATAAATTTGCAGCCTTTTATGATGATAAATTAATTGCTGTTTACATGAAACATCCAGAAAAAGCAGGA
ATTTGGAAACCAAATAAAGTATTGGTTTAA

Upstream 100 bases:

>100_bases
GAGAAATTATTGACATTCTGTCAGTAATTTTTTTTATGGAAAGGACCAATCATAAAGACTGTAAGTGATTTTTTAGTCAA
AAAATGCTATAATTTTCAGG

Downstream 100 bases:

>100_bases
TTCTTAGCAATATGAGCCAAAGTGAGAAAAATGGAAATATTAGAATTTAATGATGAACTAAAATTTGATGAAGAACTTAT
CCTTGTCCTCGGTTATTTTG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNENLNGILNVYKEAGWTSFDVVAKLRGILKTKKIGHGGTLDPSVTGVLPVAVGKSTRLLEYMEAAGKIYEGEVTIGFST
ETEDADGEIVSKTPVERELTESEIDSVMKNFIGKIKQIPPMYSAVKINGKKLYEYARAGQTVDRPTREITIKSFVRTSPM
MTDEAAGLVSFSFKVECSKGTYVRTLAVDLAEQLGYAGHMSKLQRTASNGLMIKDAVKLSEIEVAMEAGELSSLMYPAEY
AVSDLPRVNLTADQFEMARVGKKFAEDDWVTDETTDSPVIKSNKSKFLLTDLSELTRDKFAAFYDDKLIAVYMKHPEKAG
IWKPNKVLV

Sequences:

>Translated_329_residues
MNENLNGILNVYKEAGWTSFDVVAKLRGILKTKKIGHGGTLDPSVTGVLPVAVGKSTRLLEYMEAAGKIYEGEVTIGFST
ETEDADGEIVSKTPVERELTESEIDSVMKNFIGKIKQIPPMYSAVKINGKKLYEYARAGQTVDRPTREITIKSFVRTSPM
MTDEAAGLVSFSFKVECSKGTYVRTLAVDLAEQLGYAGHMSKLQRTASNGLMIKDAVKLSEIEVAMEAGELSSLMYPAEY
AVSDLPRVNLTADQFEMARVGKKFAEDDWVTDETTDSPVIKSNKSKFLLTDLSELTRDKFAAFYDDKLIAVYMKHPEKAG
IWKPNKVLV
>Mature_329_residues
MNENLNGILNVYKEAGWTSFDVVAKLRGILKTKKIGHGGTLDPSVTGVLPVAVGKSTRLLEYMEAAGKIYEGEVTIGFST
ETEDADGEIVSKTPVERELTESEIDSVMKNFIGKIKQIPPMYSAVKINGKKLYEYARAGQTVDRPTREITIKSFVRTSPM
MTDEAAGLVSFSFKVECSKGTYVRTLAVDLAEQLGYAGHMSKLQRTASNGLMIKDAVKLSEIEVAMEAGELSSLMYPAEY
AVSDLPRVNLTADQFEMARVGKKFAEDDWVTDETTDSPVIKSNKSKFLLTDLSELTRDKFAAFYDDKLIAVYMKHPEKAG
IWKPNKVLV

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=291, Percent_Identity=28.8659793814433, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI4503337, Length=210, Percent_Identity=30, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI215599015, Length=210, Percent_Identity=30, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI2367200, Length=256, Percent_Identity=36.328125, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17553978, Length=203, Percent_Identity=29.064039408867, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=130, Percent_Identity=43.0769230769231, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323204, Length=210, Percent_Identity=31.9047619047619, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI281364189, Length=202, Percent_Identity=28.7128712871287, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281364187, Length=202, Percent_Identity=28.7128712871287, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281364185, Length=202, Percent_Identity=28.7128712871287, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281364183, Length=202, Percent_Identity=28.7128712871287, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI62471759, Length=202, Percent_Identity=28.7128712871287, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17975520, Length=202, Percent_Identity=28.7128712871287, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_LACLA (Q9CGH0)

Other databases:

- EMBL:   AE005176
- PIR:   F86765
- RefSeq:   NP_267282.1
- ProteinModelPortal:   Q9CGH0
- SMR:   Q9CGH0
- GeneID:   1114766
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0328
- NMPDR:   fig|272623.1.peg.1158
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   PRK02484
- BioCyc:   LLAC272623:L0328-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 36368; Mature: 36368

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: ACT_SITE 42-42

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNENLNGILNVYKEAGWTSFDVVAKLRGILKTKKIGHGGTLDPSVTGVLPVAVGKSTRLL
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHH
EYMEAAGKIYEGEVTIGFSTETEDADGEIVSKTPVERELTESEIDSVMKNFIGKIKQIPP
HHHHHCCCEEECEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCC
MYSAVKINGKKLYEYARAGQTVDRPTREITIKSFVRTSPMMTDEAAGLVSFSFKVECSKG
CHHEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCC
TYVRTLAVDLAEQLGYAGHMSKLQRTASNGLMIKDAVKLSEIEVAMEAGELSSLMYPAEY
CEEHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHH
AVSDLPRVNLTADQFEMARVGKKFAEDDWVTDETTDSPVIKSNKSKFLLTDLSELTRDKF
HHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCEEEEHHHHHHHHHHH
AAFYDDKLIAVYMKHPEKAGIWKPNKVLV
HHHHCCCEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MNENLNGILNVYKEAGWTSFDVVAKLRGILKTKKIGHGGTLDPSVTGVLPVAVGKSTRLL
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHH
EYMEAAGKIYEGEVTIGFSTETEDADGEIVSKTPVERELTESEIDSVMKNFIGKIKQIPP
HHHHHCCCEEECEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCC
MYSAVKINGKKLYEYARAGQTVDRPTREITIKSFVRTSPMMTDEAAGLVSFSFKVECSKG
CHHEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCC
TYVRTLAVDLAEQLGYAGHMSKLQRTASNGLMIKDAVKLSEIEVAMEAGELSSLMYPAEY
CEEHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHH
AVSDLPRVNLTADQFEMARVGKKFAEDDWVTDETTDSPVIKSNKSKFLLTDLSELTRDKF
HHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCEEEEHHHHHHHHHHH
AAFYDDKLIAVYMKHPEKAGIWKPNKVLV
HHHHCCCEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471