Click here to switch to the map view.

The map label for this gene is glyS

Identifier: 15673078

GI number: 15673078

Start: 1102793

End: 1104814

Strand: Direct

Name: glyS

Synonym: L0360

Alternate gene names: 15673078

Gene position: 1102793-1104814 (Clockwise)

Preceding gene: 15673077

Following gene: 15673079

Centisome position: 46.62

GC content: 34.67

Gene sequence:

>2022_bases
ATGACAAATTACTTACTTGAAATTGGTTTGGAAGAAATTCCAGCACATTTGGTGACTCCTTCAATCAATCAATTAGCCGA
AAGAATGGAAACTTTTCTTAAAGAAAATCGTTTAGAATTTGATAAAATTATTAAATTTTCAACTCCACGTCGTTTAGCAA
TTATTGTTGAAGGTTTGGCAGAAGCGTCAGAAGCGATTGATGAAGAAGTTAAAGGTCCATCTGCTAAAATTGCTAAAGAT
GCGCAAGGAAATTGGTCAAAAGCGATTCAAGGTTTCTCAAGAGGACAAGGAGCTACTCCAGATGATTTAATCCTTAAGGG
TGATTATTATTATGCTAAAAAACATATTGACGGAGTAAAAGCAGAAGAAATTCTGTCTAAAGTTGGCGATGAAGTCATCG
CAAAAATGACATTCTCAACTTATATGAAATGGGGAAATAATGATTTTCTCTTTGTTCGTCCGATTCAATGGATTGTTTCA
CTTCTAGAAGATAAAGTTGTTGCTTTTGATTTATTGGACGTAACAGCAAATCGTTTTTCACGAGGCCATCGTTTCTTGGC
AAATGTTGAGGTTGAACTCAAAAATGCAAATGATTATGCTAGCAAAATGCCAGAAAACTTTGTTTTAGTTGATGCTGAAC
ATCGTAAAGCAGAAATTTCAGCTCAAATTTTGGCTCTTGCGTCTGAAAATAAATGGCAAGTTACTTTGCATAAAGATTTA
TTAGAAGAAGTAAATAATATTGTTGAATATCCAACTGCCTTTGTTGGTTCATTTGATCCCAAATATCTTTCTGTTCCAGC
AGAAGTTTTAGTGACATCAATGCGTGATAATCAACGTTATTTTGAAGTTTATAACCAAGAAGGTCAACTCGCACCAAACT
TTATCTCTGTCCGTAATGGGAACGCTGAAAATATTGAAAATGTAGTGCTCGGAAATGAAAAAGTTTTGGTTGCTCGTCTT
GAAGATGCAGAGTTTTTCTGGAAAGAAGACCAAAAACTTAAAATTGAAGATTTGGTCGCTAAACTTGCTAAAGTAACTTT
CCATGCTAAAATTGGTTCAATTACTGAACACATGGCGCGAACAAAAGAAATAGCCGCTAAATTAGCTGATATTGCTGGCC
TGACTGATGAAGAAAAAAGAGACGTTGCTCGTAGTGCTGAAATTTATAAATTTGACCTTTTGACAGGAATGGTTGGAGAA
TTTGATGAATTACAAGGGGTAATGGGTGAAAAATATGCGCTTTTAGCTGGTGAAAATGCAAATGTTTCTGCAGCAATTCG
TGAACATTATATGCCAACTTCAGCCGAGGGAGAATTGCCTGAAACAAAAGTTGGTTCTGTGCTTGCAGCAGCTGACAAAA
TTGATTCAGTCCTCTCTTTCTTTAATGTTGGTTTAATTCCTTCTGGTTCAAATGACCCTTATGCCCTACGTCGAGCAGTA
CAAGGCTTGATTCGTATTATTGAAAAAATGAATTGGCATTTCGATTTATCTTTATTTATCGACCAATTTGAAGGTGAAAA
TCATCTTGAAATTTTGGATTTCGTCAAAGCACGTATTCAAAAACTCTTGCTTGAAAAACTCGATCGTCATGATATTGTTG
AAGCGGCAATTAATAGTTCAAACTTTGATATTACAAACATGATGGAATCGGCTTTTGTCATTGATGGGCACAAACTTCAC
GAACCTTTTAAACCAGCAATTGAAAATGTTTCGCGTTCAATTAATTTGGTTAAAAAAGCAAAAGATATTAAAGAAATAAA
TCCAACACTTTTTGAAGAAGATGCAGAAGAAGCTCTTTATAACGTTGTTATTTCTCTTCAAAATCAATGGACTTATATGC
CTGGTGAAGAAAAATTCAGAGCAATTGTTCATTCACTTGCACCTGCAATTGAAACTTTCTTTGAAAGTGTAATGGTAATG
GCTGAAGATTTATCAGTTCGTGATAATCGTATTGCGTTGTTGTCAGAAGTTGTTGCATTAACAAGTGTTATGGCTGATTT
TTCTTTGATTAATACAAAATAA

Upstream 100 bases:

>100_bases
CAAAACTTGGTTTCCCGCTCTTAAAAGACGAAGCTTTACGTGAAAAATACCTTGGTAAAAAAGGAAAATATACTAAGATT
GTAGAAGGAGAAGAAAAATA

Downstream 100 bases:

>100_bases
AATTGATAAATTTACTGACAGAAAGTTTATTTATGACTTTAAAAAGCTGTCAGTAAAATTTGAGTTCTGTCAGTAATTTT
AACAATATTCATGAAGAAAG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MTNYLLEIGLEEIPAHLVTPSINQLAERMETFLKENRLEFDKIIKFSTPRRLAIIVEGLAEASEAIDEEVKGPSAKIAKD
AQGNWSKAIQGFSRGQGATPDDLILKGDYYYAKKHIDGVKAEEILSKVGDEVIAKMTFSTYMKWGNNDFLFVRPIQWIVS
LLEDKVVAFDLLDVTANRFSRGHRFLANVEVELKNANDYASKMPENFVLVDAEHRKAEISAQILALASENKWQVTLHKDL
LEEVNNIVEYPTAFVGSFDPKYLSVPAEVLVTSMRDNQRYFEVYNQEGQLAPNFISVRNGNAENIENVVLGNEKVLVARL
EDAEFFWKEDQKLKIEDLVAKLAKVTFHAKIGSITEHMARTKEIAAKLADIAGLTDEEKRDVARSAEIYKFDLLTGMVGE
FDELQGVMGEKYALLAGENANVSAAIREHYMPTSAEGELPETKVGSVLAAADKIDSVLSFFNVGLIPSGSNDPYALRRAV
QGLIRIIEKMNWHFDLSLFIDQFEGENHLEILDFVKARIQKLLLEKLDRHDIVEAAINSSNFDITNMMESAFVIDGHKLH
EPFKPAIENVSRSINLVKKAKDIKEINPTLFEEDAEEALYNVVISLQNQWTYMPGEEKFRAIVHSLAPAIETFFESVMVM
AEDLSVRDNRIALLSEVVALTSVMADFSLINTK

Sequences:

>Translated_673_residues
MTNYLLEIGLEEIPAHLVTPSINQLAERMETFLKENRLEFDKIIKFSTPRRLAIIVEGLAEASEAIDEEVKGPSAKIAKD
AQGNWSKAIQGFSRGQGATPDDLILKGDYYYAKKHIDGVKAEEILSKVGDEVIAKMTFSTYMKWGNNDFLFVRPIQWIVS
LLEDKVVAFDLLDVTANRFSRGHRFLANVEVELKNANDYASKMPENFVLVDAEHRKAEISAQILALASENKWQVTLHKDL
LEEVNNIVEYPTAFVGSFDPKYLSVPAEVLVTSMRDNQRYFEVYNQEGQLAPNFISVRNGNAENIENVVLGNEKVLVARL
EDAEFFWKEDQKLKIEDLVAKLAKVTFHAKIGSITEHMARTKEIAAKLADIAGLTDEEKRDVARSAEIYKFDLLTGMVGE
FDELQGVMGEKYALLAGENANVSAAIREHYMPTSAEGELPETKVGSVLAAADKIDSVLSFFNVGLIPSGSNDPYALRRAV
QGLIRIIEKMNWHFDLSLFIDQFEGENHLEILDFVKARIQKLLLEKLDRHDIVEAAINSSNFDITNMMESAFVIDGHKLH
EPFKPAIENVSRSINLVKKAKDIKEINPTLFEEDAEEALYNVVISLQNQWTYMPGEEKFRAIVHSLAPAIETFFESVMVM
AEDLSVRDNRIALLSEVVALTSVMADFSLINTK
>Mature_672_residues
TNYLLEIGLEEIPAHLVTPSINQLAERMETFLKENRLEFDKIIKFSTPRRLAIIVEGLAEASEAIDEEVKGPSAKIAKDA
QGNWSKAIQGFSRGQGATPDDLILKGDYYYAKKHIDGVKAEEILSKVGDEVIAKMTFSTYMKWGNNDFLFVRPIQWIVSL
LEDKVVAFDLLDVTANRFSRGHRFLANVEVELKNANDYASKMPENFVLVDAEHRKAEISAQILALASENKWQVTLHKDLL
EEVNNIVEYPTAFVGSFDPKYLSVPAEVLVTSMRDNQRYFEVYNQEGQLAPNFISVRNGNAENIENVVLGNEKVLVARLE
DAEFFWKEDQKLKIEDLVAKLAKVTFHAKIGSITEHMARTKEIAAKLADIAGLTDEEKRDVARSAEIYKFDLLTGMVGEF
DELQGVMGEKYALLAGENANVSAAIREHYMPTSAEGELPETKVGSVLAAADKIDSVLSFFNVGLIPSGSNDPYALRRAVQ
GLIRIIEKMNWHFDLSLFIDQFEGENHLEILDFVKARIQKLLLEKLDRHDIVEAAINSSNFDITNMMESAFVIDGHKLHE
PFKPAIENVSRSINLVKKAKDIKEINPTLFEEDAEEALYNVVISLQNQWTYMPGEEKFRAIVHSLAPAIETFFESVMVMA
EDLSVRDNRIALLSEVVALTSVMADFSLINTK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=693, Percent_Identity=33.044733044733, Blast_Score=350, Evalue=1e-97,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_LACLA (Q9CGK0)

Other databases:

- EMBL:   AE005176
- PIR:   H86761
- RefSeq:   NP_267252.1
- ProteinModelPortal:   Q9CGK0
- GeneID:   1114736
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0360
- NMPDR:   fig|272623.1.peg.1128
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- BioCyc:   LLAC272623:L0360-MONOMER
- BRENDA:   6.1.1.14
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 75718; Mature: 75587

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNYLLEIGLEEIPAHLVTPSINQLAERMETFLKENRLEFDKIIKFSTPRRLAIIVEGLA
CCCHHHCCCHHHCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHH
EASEAIDEEVKGPSAKIAKDAQGNWSKAIQGFSRGQGATPDDLILKGDYYYAKKHIDGVK
HHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHCCCCC
AEEILSKVGDEVIAKMTFSTYMKWGNNDFLFVRPIQWIVSLLEDKVVAFDLLDVTANRFS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHCHHHHHHHHHHHHHHHH
RGHRFLANVEVELKNANDYASKMPENFVLVDAEHRKAEISAQILALASENKWQVTLHKDL
CCCEEEEEEEEEEECCCHHHHHCCCCEEEEECCCCHHHHHHHHEEEECCCCEEEEEHHHH
LEEVNNIVEYPTAFVGSFDPKYLSVPAEVLVTSMRDNQRYFEVYNQEGQLAPNFISVRNG
HHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEECCC
NAENIENVVLGNEKVLVARLEDAEFFWKEDQKLKIEDLVAKLAKVTFHAKIGSITEHMAR
CHHCCCCEEECCCEEEEEEECCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
TKEIAAKLADIAGLTDEEKRDVARSAEIYKFDLLTGMVGEFDELQGVMGEKYALLAGENA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHEEHHHHHHHHCCHHHHHHHCCCCEEEEECCCC
NVSAAIREHYMPTSAEGELPETKVGSVLAAADKIDSVLSFFNVGLIPSGSNDPYALRRAV
CHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
QGLIRIIEKMNWHFDLSLFIDQFEGENHLEILDFVKARIQKLLLEKLDRHDIVEAAINSS
HHHHHHHHHCCCCEEHEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCC
NFDITNMMESAFVIDGHKLHEPFKPAIENVSRSINLVKKAKDIKEINPTLFEEDAEEALY
CCCHHHHHHHHEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NVVISLQNQWTYMPGEEKFRAIVHSLAPAIETFFESVMVMAEDLSVRDNRIALLSEVVAL
HHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
TSVMADFSLINTK
HHHHHHHHHHCCC
>Mature Secondary Structure 
TNYLLEIGLEEIPAHLVTPSINQLAERMETFLKENRLEFDKIIKFSTPRRLAIIVEGLA
CCHHHCCCHHHCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHH
EASEAIDEEVKGPSAKIAKDAQGNWSKAIQGFSRGQGATPDDLILKGDYYYAKKHIDGVK
HHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHCCCCC
AEEILSKVGDEVIAKMTFSTYMKWGNNDFLFVRPIQWIVSLLEDKVVAFDLLDVTANRFS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHCHHHHHHHHHHHHHHHH
RGHRFLANVEVELKNANDYASKMPENFVLVDAEHRKAEISAQILALASENKWQVTLHKDL
CCCEEEEEEEEEEECCCHHHHHCCCCEEEEECCCCHHHHHHHHEEEECCCCEEEEEHHHH
LEEVNNIVEYPTAFVGSFDPKYLSVPAEVLVTSMRDNQRYFEVYNQEGQLAPNFISVRNG
HHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEECCC
NAENIENVVLGNEKVLVARLEDAEFFWKEDQKLKIEDLVAKLAKVTFHAKIGSITEHMAR
CHHCCCCEEECCCEEEEEEECCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
TKEIAAKLADIAGLTDEEKRDVARSAEIYKFDLLTGMVGEFDELQGVMGEKYALLAGENA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHEEHHHHHHHHCCHHHHHHHCCCCEEEEECCCC
NVSAAIREHYMPTSAEGELPETKVGSVLAAADKIDSVLSFFNVGLIPSGSNDPYALRRAV
CHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
QGLIRIIEKMNWHFDLSLFIDQFEGENHLEILDFVKARIQKLLLEKLDRHDIVEAAINSS
HHHHHHHHHCCCCEEHEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCC
NFDITNMMESAFVIDGHKLHEPFKPAIENVSRSINLVKKAKDIKEINPTLFEEDAEEALY
CCCHHHHHHHHEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NVVISLQNQWTYMPGEEKFRAIVHSLAPAIETFFESVMVMAEDLSVRDNRIALLSEVVAL
HHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
TSVMADFSLINTK
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471