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The map label for this gene is pi212

Identifier: 15673001

GI number: 15673001

Start: 1043169

End: 1043621

Strand: Direct

Name: pi212

Synonym: L0300

Alternate gene names: 15673001

Gene position: 1043169-1043621 (Clockwise)

Preceding gene: 15673000

Following gene: 15673002

Centisome position: 44.1

GC content: 38.85

Gene sequence:

>453_bases
ATGATTAATAATGTTGTATTAGTAGGTCGCTTAACTCGTGACCCTGAACTTAGACATACACCACAGAACCAAGCAGTAGG
TACATTTGGATTAGCTGTAAATCGCCAGTTTAAAAATGCGAATGGCGAACGTGAGGCTGATTTCATTAACTGCGTTATTT
GGCGACAACAAGCCGAAAATTTAGCTAAGTTTGCTAAAAAAGGAGCTTTGATTGGAATAACTGGACGGATTCAAACAAGG
AACTATGAGAACCAACAAGGACAAAAAGTTTATGTTACCGAAGTTGTTGCAGATACTTTCCAAATGCTAGAAAGCAATAA
AACACAAGGTCAGCAAACAAGTAAACCGCAAGCTCAAAATAAAAAGCCACAAGCACCAGACCCTTTTAAAGCTCCTGCTG
CTGATCCATTTGCTGGTGGTACTGAAATTTCAGATGATGACCTACCATTTTAA

Upstream 100 bases:

>100_bases
AAAATCCAGAAAATAAACCTAATGTTGATGAATTTTTGAAAACAAAGTCGGAACACGAAGTTGCTGAAATGATGAAAGCA
TATATAGATTGGAGTAAATA

Downstream 100 bases:

>100_bases
TTAACAACCAGGTGCAGCGTGCGTAACAAATGCTTAAATTCGAGGGGATAGGCAATGCGCAACATCCCCCAGCCTTTAAT
TTGAAAAATAAAACTTGAAA

Product: single strand binding protein

Products: NA

Alternate protein names: SSB 1; Helix-destabilizing protein 1

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MINNVVLVGRLTRDPELRHTPQNQAVGTFGLAVNRQFKNANGEREADFINCVIWRQQAENLAKFAKKGALIGITGRIQTR
NYENQQGQKVYVTEVVADTFQMLESNKTQGQQTSKPQAQNKKPQAPDPFKAPAADPFAGGTEISDDDLPF

Sequences:

>Translated_150_residues
MINNVVLVGRLTRDPELRHTPQNQAVGTFGLAVNRQFKNANGEREADFINCVIWRQQAENLAKFAKKGALIGITGRIQTR
NYENQQGQKVYVTEVVADTFQMLESNKTQGQQTSKPQAQNKKPQAPDPFKAPAADPFAGGTEISDDDLPF
>Mature_150_residues
MINNVVLVGRLTRDPELRHTPQNQAVGTFGLAVNRQFKNANGEREADFINCVIWRQQAENLAKFAKKGALIGITGRIQTR
NYENQQGQKVYVTEVVADTFQMLESNKTQGQQTSKPQAQNKKPQAPDPFKAPAADPFAGGTEISDDDLPF

Specific function: This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair

COG id: COG0629

COG function: function code L; Single-stranded DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SSB domain

Homologues:

Organism=Escherichia coli, GI1790494, Length=115, Percent_Identity=34.7826086956522, Blast_Score=71, Evalue=3e-14,

Paralogues:

None

Copy number: 1622 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SSB1_LACLA (Q9CGS5)

Other databases:

- EMBL:   AE005176
- PIR:   C86752
- RefSeq:   NP_267175.1
- ProteinModelPortal:   Q9CGS5
- SMR:   Q9CGS5
- GeneID:   1114651
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0300
- NMPDR:   fig|272623.1.peg.1043
- HOGENOM:   HBG755544
- OMA:   PSNIANA
- BioCyc:   LLAC272623:L0300-MONOMER
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000424
- InterPro:   IPR011344
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR10302
- TIGRFAMs:   TIGR00621

Pfam domain/function: PF00436 SSB; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 16644; Mature: 16644

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50935 SSB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINNVVLVGRLTRDPELRHTPQNQAVGTFGLAVNRQFKNANGEREADFINCVIWRQQAEN
CCCCEEEEEECCCCCCCCCCCCCCCEEEEHHEEECHHCCCCCCCCHHHHHHHHHHHHHHH
LAKFAKKGALIGITGRIQTRNYENQQGQKVYVTEVVADTFQMLESNKTQGQQTSKPQAQN
HHHHHHCCCEEEEECEEECCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KKPQAPDPFKAPAADPFAGGTEISDDDLPF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MINNVVLVGRLTRDPELRHTPQNQAVGTFGLAVNRQFKNANGEREADFINCVIWRQQAEN
CCCCEEEEEECCCCCCCCCCCCCCCEEEEHHEEECHHCCCCCCCCHHHHHHHHHHHHHHH
LAKFAKKGALIGITGRIQTRNYENQQGQKVYVTEVVADTFQMLESNKTQGQQTSKPQAQN
HHHHHHCCCEEEEECEEECCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KKPQAPDPFKAPAADPFAGGTEISDDDLPF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471