Click here to switch to the map view.

The map label for this gene is dacB

Identifier: 15672932

GI number: 15672932

Start: 975393

End: 976139

Strand: Direct

Name: dacB

Synonym: L175147

Alternate gene names: 15672932

Gene position: 975393-976139 (Clockwise)

Preceding gene: 15672931

Following gene: 15672933

Centisome position: 41.23

GC content: 34.94

Gene sequence:

>747_bases
ATGAAAAGAAGAATAACATTTAGTTTAATCGTGCTTGTTCTTTTAATCATTGGCGCTTTAGGATTTATTTTAATCACCAA
TGATAAGTCAAATTCAACAAACGAATCAGCTAAAAAAACAAGTATTTCGTCATCTAAAGAGGTGGCAAAGAAAAAGGATT
TACCTGACGCTAAACAATCAGATTGGGATTTAGTATTGGTGAGTCGAAAATCACCTAAAGATGAAATGAACCCAACACTG
GCAACCGTAAATGGGATTCAAGTTGATCAAAGGATAGCATCAGCGGTTCAACAATTTTTAGCAGCAGCCCAACAAATATC
ACCAGCTGAACATTTAATCTCAGGTTATCGGTCAGTCTCATATCAAGAAGGGCTTTATCAATCTTATGTCCAAGCAGAAA
TGAATGGTCAAGGAACAGTAAATTCTAGTGGAAATCCAATTTCAGAAGAAGAAGCGATAAAAAATGTACAAACTTATTCT
CAGCCGCCAAGAAGTTCAGAACATCAAACAGGATTAGCGATTGATATGAGTAATATTGATAGCCTAAATGAGAGTCAATA
TGCAAAACAAGTTGCAGCAATTGCTCCCAAATATGGATTTGTTCTTCGTTTTCCAGAAGGGAAAAAGGCAGAGACAGGAA
TTGGTTATGAAGACTGGCATTTTAGATATGTTGGTTTAGAAAATGCTAAATATATGACCGAACACAATCTTACTTTAGAA
GAATATCTAAAACTATTACCAGCATAA

Upstream 100 bases:

>100_bases
GTTCTGTCACCCATTTAAGTTTTGAGGATGGAAAATTCACCATTGGAAAAGTCGGAGACATGTCTTATCGAGAAATAGGG
AGTGAAAAGTTAGCTAAAAA

Downstream 100 bases:

>100_bases
AGGAAATGAAATCATGCTAAACCGAACCAGACTTCTACTCTATGTTAATGTTGCTCTATTTATTTACAGTGTCTCGGAAA
TGATTAAAGTTCTCCTTGTT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKRRITFSLIVLVLLIIGALGFILITNDKSNSTNESAKKTSISSSKEVAKKKDLPDAKQSDWDLVLVSRKSPKDEMNPTL
ATVNGIQVDQRIASAVQQFLAAAQQISPAEHLISGYRSVSYQEGLYQSYVQAEMNGQGTVNSSGNPISEEEAIKNVQTYS
QPPRSSEHQTGLAIDMSNIDSLNESQYAKQVAAIAPKYGFVLRFPEGKKAETGIGYEDWHFRYVGLENAKYMTEHNLTLE
EYLKLLPA

Sequences:

>Translated_248_residues
MKRRITFSLIVLVLLIIGALGFILITNDKSNSTNESAKKTSISSSKEVAKKKDLPDAKQSDWDLVLVSRKSPKDEMNPTL
ATVNGIQVDQRIASAVQQFLAAAQQISPAEHLISGYRSVSYQEGLYQSYVQAEMNGQGTVNSSGNPISEEEAIKNVQTYS
QPPRSSEHQTGLAIDMSNIDSLNESQYAKQVAAIAPKYGFVLRFPEGKKAETGIGYEDWHFRYVGLENAKYMTEHNLTLE
EYLKLLPA
>Mature_248_residues
MKRRITFSLIVLVLLIIGALGFILITNDKSNSTNESAKKTSISSSKEVAKKKDLPDAKQSDWDLVLVSRKSPKDEMNPTL
ATVNGIQVDQRIASAVQQFLAAAQQISPAEHLISGYRSVSYQEGLYQSYVQAEMNGQGTVNSSGNPISEEEAIKNVQTYS
QPPRSSEHQTGLAIDMSNIDSLNESQYAKQVAAIAPKYGFVLRFPEGKKAETGIGYEDWHFRYVGLENAKYMTEHNLTLE
EYLKLLPA

Specific function: Vancomycin-inducible, penicillin-resistant, DD- carboxypeptidase that hydrolyzes depsipeptide- and D-alanyl-D- alanine-containing peptidoglycan precursors. Insensitive to beta- lactams [H]

COG id: COG1876

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M15B family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR003709 [H]

Pfam domain/function: PF02557 VanY [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 27478; Mature: 27478

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRITFSLIVLVLLIIGALGFILITNDKSNSTNESAKKTSISSSKEVAKKKDLPDAKQS
CCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHCCHHHHHHHHCCCCCCCCC
DWDLVLVSRKSPKDEMNPTLATVNGIQVDQRIASAVQQFLAAAQQISPAEHLISGYRSVS
CCCEEEEECCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
YQEGLYQSYVQAEMNGQGTVNSSGNPISEEEAIKNVQTYSQPPRSSEHQTGLAIDMSNID
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCC
SLNESQYAKQVAAIAPKYGFVLRFPEGKKAETGIGYEDWHFRYVGLENAKYMTEHNLTLE
CCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEHHHCCCCHH
EYLKLLPA
HHHHHCCC
>Mature Secondary Structure
MKRRITFSLIVLVLLIIGALGFILITNDKSNSTNESAKKTSISSSKEVAKKKDLPDAKQS
CCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHCCHHHHHHHHCCCCCCCCC
DWDLVLVSRKSPKDEMNPTLATVNGIQVDQRIASAVQQFLAAAQQISPAEHLISGYRSVS
CCCEEEEECCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
YQEGLYQSYVQAEMNGQGTVNSSGNPISEEEAIKNVQTYSQPPRSSEHQTGLAIDMSNID
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCC
SLNESQYAKQVAAIAPKYGFVLRFPEGKKAETGIGYEDWHFRYVGLENAKYMTEHNLTLE
CCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEHHHCCCCHH
EYLKLLPA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8631706; 12663927 [H]