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The map label for this gene is mraW

Identifier: 15672847

GI number: 15672847

Start: 887854

End: 888807

Strand: Direct

Name: mraW

Synonym: L87561

Alternate gene names: 15672847

Gene position: 887854-888807 (Clockwise)

Preceding gene: 15672846

Following gene: 15672848

Centisome position: 37.53

GC content: 35.12

Gene sequence:

>954_bases
TTGCCAAAAACAATAGCATTTAAGCATGATACAGTCTTACTACATGAAACCGTAGATATGCTTGAAGTAAAGCCAAATGG
AATTTATGTTGATGCGACACTAGGTGGCGCAGGACATAGTGAGTATCTTCTTAGTAAACTGACTAATGGTCATCTTTATT
CATTTGACCAGGATGAAACTGCTCATGAAAACGCAAAAGTACGCTTAAGTGAGCAACTTGCAGAAGATAAAGTAACATTG
ATTAAAAGTAATTTTCGCTATCTTAAATCAGCTTTAGCTGAATTAGGAGTCACGAAAATTGATGGAATTCTTTATGATTT
AGGAGTTTCCAGCCCACAATTCGATGATAGTCAACGAGGTTTCTCTTATAAAAAAGAGGCAAGATTAGACATGCGGATGG
ACCAAAATCAAACTCTTTCTGCTTATGAAGTTGTAAATGATTATCCTTACGAAGCATTAGTTCGCATTTTTTTCCGCTAC
GGCGAAGATAAATTCTCTAAACAAATTGCTCGTAAGATTGAACAAGCAAGAAAAATTAAACCTATTGAGACAACAATTGA
GCTTGCAGATTTAATAAAATCTGCATTGCCTCAAAAAGAACTCAAGAAAAAAGGGCATCCAGCCAAACGCATTTTCCAAG
CGATTCGTATTGAAGTAAATGATGAACTTGGAGCAGCGGAAGAATCAATCGAACAAGCCATTGATTTATTAAAAGTTGAT
GGTCGAATCTCGGTAATTACTTTCCATTCTTTGGAAGATCGTTTAACAAAAACAATTTTCAAAGAATATAGTACAGTTAA
TGTTCCCAAGGGATTACCGATGCTTCCTAAAGATATGGAAGCTAAACTAAAATTGATTAATCGCAAACCAGTTCTTGCAA
GCGAAGAAGAACTCGAATTTAATAATCGAGCTCATAGTGCAAAATTAAGAGTAGCGCAAAAACAAAGAGATTAA

Upstream 100 bases:

>100_bases
CCTTTTTATTAAAAAAATCTTTACATAATCATGTAAACTACTACAAAAATAGAAAAAAAGTGATATAATAAACTGTAAGT
ATACAAGGAGGACGGCGCTC

Downstream 100 bases:

>100_bases
TCAAATAACAAGTTGGTCTAATAAAGAGGGTTTTCCCACTTGAGGATTGTTTTTGGATGTCTTATATAAATGAAATAACA
ATATTTAGCTTTTCTCTTTT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MPKTIAFKHDTVLLHETVDMLEVKPNGIYVDATLGGAGHSEYLLSKLTNGHLYSFDQDETAHENAKVRLSEQLAEDKVTL
IKSNFRYLKSALAELGVTKIDGILYDLGVSSPQFDDSQRGFSYKKEARLDMRMDQNQTLSAYEVVNDYPYEALVRIFFRY
GEDKFSKQIARKIEQARKIKPIETTIELADLIKSALPQKELKKKGHPAKRIFQAIRIEVNDELGAAEESIEQAIDLLKVD
GRISVITFHSLEDRLTKTIFKEYSTVNVPKGLPMLPKDMEAKLKLINRKPVLASEEELEFNNRAHSAKLRVAQKQRD

Sequences:

>Translated_317_residues
MPKTIAFKHDTVLLHETVDMLEVKPNGIYVDATLGGAGHSEYLLSKLTNGHLYSFDQDETAHENAKVRLSEQLAEDKVTL
IKSNFRYLKSALAELGVTKIDGILYDLGVSSPQFDDSQRGFSYKKEARLDMRMDQNQTLSAYEVVNDYPYEALVRIFFRY
GEDKFSKQIARKIEQARKIKPIETTIELADLIKSALPQKELKKKGHPAKRIFQAIRIEVNDELGAAEESIEQAIDLLKVD
GRISVITFHSLEDRLTKTIFKEYSTVNVPKGLPMLPKDMEAKLKLINRKPVLASEEELEFNNRAHSAKLRVAQKQRD
>Mature_316_residues
PKTIAFKHDTVLLHETVDMLEVKPNGIYVDATLGGAGHSEYLLSKLTNGHLYSFDQDETAHENAKVRLSEQLAEDKVTLI
KSNFRYLKSALAELGVTKIDGILYDLGVSSPQFDDSQRGFSYKKEARLDMRMDQNQTLSAYEVVNDYPYEALVRIFFRYG
EDKFSKQIARKIEQARKIKPIETTIELADLIKSALPQKELKKKGHPAKRIFQAIRIEVNDELGAAEESIEQAIDLLKVDG
RISVITFHSLEDRLTKTIFKEYSTVNVPKGLPMLPKDMEAKLKLINRKPVLASEEELEFNNRAHSAKLRVAQKQRD

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=343, Percent_Identity=34.402332361516, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI165377202, Length=195, Percent_Identity=38.4615384615385, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1786270, Length=316, Percent_Identity=40.1898734177215, Blast_Score=223, Evalue=1e-59,
Organism=Drosophila melanogaster, GI62472493, Length=332, Percent_Identity=35.5421686746988, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI28571637, Length=332, Percent_Identity=35.5421686746988, Blast_Score=175, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_LACLA (Q9CH73)

Other databases:

- EMBL:   AE005176
- PIR:   A86733
- RefSeq:   NP_267021.1
- ProteinModelPortal:   Q9CH73
- SMR:   Q9CH73
- GeneID:   1114494
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L87561
- NMPDR:   fig|272623.1.peg.886
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   LLAC272623:L87561-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 36110; Mature: 35979

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: BINDING 56-56 BINDING 85-85 BINDING 106-106 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTIAFKHDTVLLHETVDMLEVKPNGIYVDATLGGAGHSEYLLSKLTNGHLYSFDQDET
CCCCEEECCCEEEEECHHHHEEECCCCEEEEEEECCCCHHHHHHHHHCCCCEEECCCCCC
AHENAKVRLSEQLAEDKVTLIKSNFRYLKSALAELGVTKIDGILYDLGVSSPQFDDSQRG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEECCCCCCCCCCCCCC
FSYKKEARLDMRMDQNQTLSAYEVVNDYPYEALVRIFFRYGEDKFSKQIARKIEQARKIK
CCCHHHCCHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
PIETTIELADLIKSALPQKELKKKGHPAKRIFQAIRIEVNDELGAAEESIEQAIDLLKVD
CHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCC
GRISVITFHSLEDRLTKTIFKEYSTVNVPKGLPMLPKDMEAKLKLINRKPVLASEEELEF
CEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHH
NNRAHSAKLRVAQKQRD
CCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PKTIAFKHDTVLLHETVDMLEVKPNGIYVDATLGGAGHSEYLLSKLTNGHLYSFDQDET
CCCEEECCCEEEEECHHHHEEECCCCEEEEEEECCCCHHHHHHHHHCCCCEEECCCCCC
AHENAKVRLSEQLAEDKVTLIKSNFRYLKSALAELGVTKIDGILYDLGVSSPQFDDSQRG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEECCCCCCCCCCCCCC
FSYKKEARLDMRMDQNQTLSAYEVVNDYPYEALVRIFFRYGEDKFSKQIARKIEQARKIK
CCCHHHCCHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
PIETTIELADLIKSALPQKELKKKGHPAKRIFQAIRIEVNDELGAAEESIEQAIDLLKVD
CHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCC
GRISVITFHSLEDRLTKTIFKEYSTVNVPKGLPMLPKDMEAKLKLINRKPVLASEEELEF
CEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHH
NNRAHSAKLRVAQKQRD
CCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471