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The map label for this gene is metB2

Identifier: 15672763

GI number: 15672763

Start: 790537

End: 791679

Strand: Direct

Name: metB2

Synonym: L0181

Alternate gene names: 15672763

Gene position: 790537-791679 (Clockwise)

Preceding gene: 15672762

Following gene: 15672764

Centisome position: 33.42

GC content: 37.36

Gene sequence:

>1143_bases
ATGACAAGTATAAAAACGAAAGTAATTCATGGTGGTATTTCAACAGACAAGACAACAGGAGCAGTATCTGTTCCGATTTA
TCAAACGTCAACTTATAAACAAAACGGACTTGGACAACCTAAAGAATACGAATATTCACGTTCTGGAAATCCAACGCGTC
ATGCCTTGGAAGAATTAATTGCAGATTTAGAAGGTGGTGTTCAAGGATTTGCATTTAGTTCAGGACTTGCTGGAATTCAT
GCGGTTCTTTCGCTATTTTCTGCAGGAGATCATATCATTTTAGCTGATGATGTTTATGGTGGAACTTTCCGTTTGATGGA
TAAAGTGCTGACAAAGACAGGGATTATTTATGATTTAGTTGATTTGAGTAATCTTGATGACTTGAAAGCGGCTTTTAAAG
AAGAAACGAAAGCTATTTATTTTGAAACTCCTTCAAATCCTTTATTAAAAGTTTTGGACATTAAGGAAATTTCAGCAATT
GCTAAAGCTCATGATGCCTTAACACTGGTGGATAATACCTTTGCGACACCGTATTTACAACAACCAATTGCTTTAGGAGC
TGATATTGTTTTGCATTCCGCAACGAAATATTTAGGTGGACATTCAGATGTAGTGGCAGGACTAGTTACAACCAATTCAA
AAGAACTCGCCAGCGAAATTGGCTTTTTACAAAATTCAATTGGGGCGGTTCTTGGACCACAAGACAGCTGGTTAGTGCAA
AGAGGTATTAAAACACTTGCGCTTCGGATGGAGGCTCATTCGGCAAATGCTCAAAAAATTGCTGAGTTTTTAGAAACTTC
TAAAGCCGTCTCAAAAGTTTATTATCCAGGTCTTAATAGCCATCCGGGGCATGAAATTGCTAAAAAACAAATGTCTGCCT
TTGGAGGGATGATTTCATTTGAATTAACGGATGAAAATGCCGTCAAAGATTTTGTTGAAAACTTGAGCTATTTTACGTTG
GCTGAATCACTAGGTGGCGTTGAATCTTTAATTGAAGTGCCAGCAGTGATGACTCATGCTTCTATTCCAAAAGAACTTCG
TGAAGAAATTGGGATTAAGGATGGCCTAATTCGTCTATCAGTAGGGGTTGAAGCAATTGAAGATTTACTGACAGATATCA
AAGAAGCACTTGAAAAAAAATAA

Upstream 100 bases:

>100_bases
TACATAAAAAAAGTTAATTCTGCTATAAAAAAATCTTATAGCACTGGGCACACTTTCGTGATATCTTTATATTAGATTGA
AAATAGAAAGAAAAGGGCCA

Downstream 100 bases:

>100_bases
TTTTACTGACAGACTTGTAAGCAAAGCTTACGACTCCTTTCTCCCTCACTTTAACAGCTCACATTGGTCCTGTTGAACTG
ACAGAAAAATTAGACAGTAG

Product: cystathionine gamma-synthase/cystathionine beta-lyase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTSIKTKVIHGGISTDKTTGAVSVPIYQTSTYKQNGLGQPKEYEYSRSGNPTRHALEELIADLEGGVQGFAFSSGLAGIH
AVLSLFSAGDHIILADDVYGGTFRLMDKVLTKTGIIYDLVDLSNLDDLKAAFKEETKAIYFETPSNPLLKVLDIKEISAI
AKAHDALTLVDNTFATPYLQQPIALGADIVLHSATKYLGGHSDVVAGLVTTNSKELASEIGFLQNSIGAVLGPQDSWLVQ
RGIKTLALRMEAHSANAQKIAEFLETSKAVSKVYYPGLNSHPGHEIAKKQMSAFGGMISFELTDENAVKDFVENLSYFTL
AESLGGVESLIEVPAVMTHASIPKELREEIGIKDGLIRLSVGVEAIEDLLTDIKEALEKK

Sequences:

>Translated_380_residues
MTSIKTKVIHGGISTDKTTGAVSVPIYQTSTYKQNGLGQPKEYEYSRSGNPTRHALEELIADLEGGVQGFAFSSGLAGIH
AVLSLFSAGDHIILADDVYGGTFRLMDKVLTKTGIIYDLVDLSNLDDLKAAFKEETKAIYFETPSNPLLKVLDIKEISAI
AKAHDALTLVDNTFATPYLQQPIALGADIVLHSATKYLGGHSDVVAGLVTTNSKELASEIGFLQNSIGAVLGPQDSWLVQ
RGIKTLALRMEAHSANAQKIAEFLETSKAVSKVYYPGLNSHPGHEIAKKQMSAFGGMISFELTDENAVKDFVENLSYFTL
AESLGGVESLIEVPAVMTHASIPKELREEIGIKDGLIRLSVGVEAIEDLLTDIKEALEKK
>Mature_379_residues
TSIKTKVIHGGISTDKTTGAVSVPIYQTSTYKQNGLGQPKEYEYSRSGNPTRHALEELIADLEGGVQGFAFSSGLAGIHA
VLSLFSAGDHIILADDVYGGTFRLMDKVLTKTGIIYDLVDLSNLDDLKAAFKEETKAIYFETPSNPLLKVLDIKEISAIA
KAHDALTLVDNTFATPYLQQPIALGADIVLHSATKYLGGHSDVVAGLVTTNSKELASEIGFLQNSIGAVLGPQDSWLVQR
GIKTLALRMEAHSANAQKIAEFLETSKAVSKVYYPGLNSHPGHEIAKKQMSAFGGMISFELTDENAVKDFVENLSYFTLA
ESLGGVESLIEVPAVMTHASIPKELREEIGIKDGLIRLSVGVEAIEDLLTDIKEALEKK

Specific function: The enzymatic degradation of amino acids in cheese is believed to generate aroma compounds and therefore to be essential for flavor development. Cystathionine beta-lyase (CBL) can convert cystathionine to homocysteine but is also able to catalyze an alpha

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family

Homologues:

Organism=Homo sapiens, GI21361334, Length=361, Percent_Identity=50.415512465374, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI299473759, Length=361, Percent_Identity=45.4293628808864, Blast_Score=301, Evalue=8e-82,
Organism=Homo sapiens, GI25453487, Length=360, Percent_Identity=45, Blast_Score=289, Evalue=3e-78,
Organism=Escherichia coli, GI1790375, Length=375, Percent_Identity=44.2666666666667, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1789383, Length=393, Percent_Identity=28.498727735369, Blast_Score=174, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17560056, Length=378, Percent_Identity=48.1481481481481, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17538055, Length=378, Percent_Identity=48.1481481481481, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=43.2432432432432, Blast_Score=286, Evalue=9e-78,
Organism=Saccharomyces cerevisiae, GI6319307, Length=384, Percent_Identity=48.4375, Blast_Score=335, Evalue=1e-92,
Organism=Saccharomyces cerevisiae, GI6321254, Length=400, Percent_Identity=35.25, Blast_Score=222, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6323333, Length=419, Percent_Identity=35.3221957040573, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321170, Length=322, Percent_Identity=34.1614906832298, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6321904, Length=400, Percent_Identity=30.5, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322970, Length=305, Percent_Identity=27.8688524590164, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322590, Length=259, Percent_Identity=27.7992277992278, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323557, Length=266, Percent_Identity=26.3157894736842, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922554, Length=377, Percent_Identity=50.132625994695, Blast_Score=369, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): METC_LACLA (P0A4K2)

Other databases:

- EMBL:   AE005176
- PIR:   E86722
- RefSeq:   NP_266937.1
- ProteinModelPortal:   P0A4K2
- SMR:   P0A4K2
- GeneID:   1114408
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0181
- NMPDR:   fig|272623.1.peg.801
- HOGENOM:   HBG754729
- OMA:   NQYEYSR
- ProtClustDB:   PRK06176
- BioCyc:   LLAC272623:L0181-MONOMER
- BRENDA:   4.4.1.8
- GO:   GO:0005737
- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11808
- PIRSF:   PIRSF001434

Pfam domain/function: PF01053 Cys_Met_Meta_PP; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =4.4.1.8

Molecular weight: Translated: 40938; Mature: 40807

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIKTKVIHGGISTDKTTGAVSVPIYQTSTYKQNGLGQPKEYEYSRSGNPTRHALEELI
CCCCCCEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
ADLEGGVQGFAFSSGLAGIHAVLSLFSAGDHIILADDVYGGTFRLMDKVLTKTGIIYDLV
HHHHCCCCCHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCHHHHHH
DLSNLDDLKAAFKEETKAIYFETPSNPLLKVLDIKEISAIAKAHDALTLVDNTFATPYLQ
CCCCCHHHHHHHHHCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHEECCCCCCCHHHH
QPIALGADIVLHSATKYLGGHSDVVAGLVTTNSKELASEIGFLQNSIGAVLGPQDSWLVQ
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCHHCCCCHHHHHH
RGIKTLALRMEAHSANAQKIAEFLETSKAVSKVYYPGLNSHPGHEIAKKQMSAFGGMISF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEE
ELTDENAVKDFVENLSYFTLAESLGGVESLIEVPAVMTHASIPKELREEIGIKDGLIRLS
EECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEH
VGVEAIEDLLTDIKEALEKK
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSIKTKVIHGGISTDKTTGAVSVPIYQTSTYKQNGLGQPKEYEYSRSGNPTRHALEELI
CCCCCEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
ADLEGGVQGFAFSSGLAGIHAVLSLFSAGDHIILADDVYGGTFRLMDKVLTKTGIIYDLV
HHHHCCCCCHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCHHHHHH
DLSNLDDLKAAFKEETKAIYFETPSNPLLKVLDIKEISAIAKAHDALTLVDNTFATPYLQ
CCCCCHHHHHHHHHCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHEECCCCCCCHHHH
QPIALGADIVLHSATKYLGGHSDVVAGLVTTNSKELASEIGFLQNSIGAVLGPQDSWLVQ
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCHHCCCCHHHHHH
RGIKTLALRMEAHSANAQKIAEFLETSKAVSKVYYPGLNSHPGHEIAKKQMSAFGGMISF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEE
ELTDENAVKDFVENLSYFTLAESLGGVESLIEVPAVMTHASIPKELREEIGIKDGLIRLS
EECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEH
VGVEAIEDLLTDIKEALEKK
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471