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The map label for this gene is nusA

Identifier: 15672746

GI number: 15672746

Start: 773810

End: 774958

Strand: Direct

Name: nusA

Synonym: L173813

Alternate gene names: 15672746

Gene position: 773810-774958 (Clockwise)

Preceding gene: 15672745

Following gene: 15672747

Centisome position: 32.71

GC content: 38.21

Gene sequence:

>1149_bases
ATGAGCAAAGAAATGCTGAACGCCTTTGCAATGTTAGAAGAGCAAAAAGGAATCAAATCTGAGATTGTTATTGAAGCGAT
TGAGCAAGCTATCACAGCAGCTTACAAGCGTCAATATGGTCAAGCACAAAATGTTAAAGTTATCTTTGATGAAAAGAAAG
GAAACTTCAATGTTTATTCGACTCGTGAAGTCGTGGATGAAGTTTTCGATAGTCGTCTTGAAATTTCACTTGAGGATGCG
CATGAATTAAATCCTCACTATGAAATTGGCGACAAAATTATGTTTGAAGAAAAACCAAAAGATTTTGCTCGTACTGCTGC
TGGAGCTGCTAAACAAGTTATCATGCAAAAAATGCGTGAAGAAGAACGCACAATTATTTATAACGAATATAGCCGTTACA
AAGATGAAATCATCCAAGGAACTGTTGAAAAAGTTGATGCTCGTGCCGTTTATGTAAATCTTGGCCGTGTTGATGCTCTT
TTGACTAAGAAAGACCAAATTCCACAAGAAAACTTCCATGTGGGAGACCGTGTAAAAGTTTATGTTACTGATGTTGTCTT
GACTCCAAAAGGAACTCGTGTCTTCATTTCACGTACTGCGCCTGATATGCTCAAACGTATGTTTGAAAAAGAAATTCCTG
AAGTTTTTGATGGAACTGTTGAAATCAAATCAATTGCTCGTGATGCTGGAGACCGTGCTAAAGTCATCGTTCAAAGTCAT
GATGAGAATGTTGATGCAATTGGTACAATGGTTGGGACTAAAGGTTCACGTATCCAAGGAATCATTCGCGAACTTGCGGG
TGAAAAAATGGATATCATTGAATGGTCAGAAGATAAAGCGACACTTGTTGCCAATGCTTTAAAACCTGCTCGTATTGAAC
AAGTTTTGATTACTGCTGACGGTTCAAGTTTAGCAGTTGTTGCGCGCGATCAATTGTCACTTGCCATTGGTAAACGTGGT
CAAAATGTTCGTCTTGCTGCACACGTTACAAACAGTAAAATTGATATTAAAGCGGCTGATGAATTCAATCTGGATGATTA
TGAATTCGTGGGTGAAGATGGGGAAGAAATTCCAACAACTGAAGTTAACGAAACTCTTGTAGTTGAAGAAGTTGTTGAAA
CACCTGAAGTTAGCGAAAGTGAAGAATAA

Upstream 100 bases:

>100_bases
CTTGAGTTAGGCGAAAATGTAAAACTTCCTTGATTCAAGGTAGTCGAACTTAGCTTATATTAAAAATAAAATTTTCTTAT
AAAAAGAAAGAGAGAAAAAA

Downstream 100 bases:

>100_bases
ATTTTAAAAGCTTGAAAATTTACTGACAGAAAATAATATTTTAGAAAAAGCACAAAGGTGCTGAAAATTTCTTTTTTATA
GGTCCGTCAGTAAAATGGCT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSKEMLNAFAMLEEQKGIKSEIVIEAIEQAITAAYKRQYGQAQNVKVIFDEKKGNFNVYSTREVVDEVFDSRLEISLEDA
HELNPHYEIGDKIMFEEKPKDFARTAAGAAKQVIMQKMREEERTIIYNEYSRYKDEIIQGTVEKVDARAVYVNLGRVDAL
LTKKDQIPQENFHVGDRVKVYVTDVVLTPKGTRVFISRTAPDMLKRMFEKEIPEVFDGTVEIKSIARDAGDRAKVIVQSH
DENVDAIGTMVGTKGSRIQGIIRELAGEKMDIIEWSEDKATLVANALKPARIEQVLITADGSSLAVVARDQLSLAIGKRG
QNVRLAAHVTNSKIDIKAADEFNLDDYEFVGEDGEEIPTTEVNETLVVEEVVETPEVSESEE

Sequences:

>Translated_382_residues
MSKEMLNAFAMLEEQKGIKSEIVIEAIEQAITAAYKRQYGQAQNVKVIFDEKKGNFNVYSTREVVDEVFDSRLEISLEDA
HELNPHYEIGDKIMFEEKPKDFARTAAGAAKQVIMQKMREEERTIIYNEYSRYKDEIIQGTVEKVDARAVYVNLGRVDAL
LTKKDQIPQENFHVGDRVKVYVTDVVLTPKGTRVFISRTAPDMLKRMFEKEIPEVFDGTVEIKSIARDAGDRAKVIVQSH
DENVDAIGTMVGTKGSRIQGIIRELAGEKMDIIEWSEDKATLVANALKPARIEQVLITADGSSLAVVARDQLSLAIGKRG
QNVRLAAHVTNSKIDIKAADEFNLDDYEFVGEDGEEIPTTEVNETLVVEEVVETPEVSESEE
>Mature_381_residues
SKEMLNAFAMLEEQKGIKSEIVIEAIEQAITAAYKRQYGQAQNVKVIFDEKKGNFNVYSTREVVDEVFDSRLEISLEDAH
ELNPHYEIGDKIMFEEKPKDFARTAAGAAKQVIMQKMREEERTIIYNEYSRYKDEIIQGTVEKVDARAVYVNLGRVDALL
TKKDQIPQENFHVGDRVKVYVTDVVLTPKGTRVFISRTAPDMLKRMFEKEIPEVFDGTVEIKSIARDAGDRAKVIVQSHD
ENVDAIGTMVGTKGSRIQGIIRELAGEKMDIIEWSEDKATLVANALKPARIEQVLITADGSSLAVVARDQLSLAIGKRGQ
NVRLAAHVTNSKIDIKAADEFNLDDYEFVGEDGEEIPTTEVNETLVVEEVVETPEVSESEE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=344, Percent_Identity=35.1744186046512, Blast_Score=234, Evalue=5e-63,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 42905; Mature: 42774

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEMLNAFAMLEEQKGIKSEIVIEAIEQAITAAYKRQYGQAQNVKVIFDEKKGNFNVYS
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEE
TREVVDEVFDSRLEISLEDAHELNPHYEIGDKIMFEEKPKDFARTAAGAAKQVIMQKMRE
HHHHHHHHHCCCEEEEECHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
EERTIIYNEYSRYKDEIIQGTVEKVDARAVYVNLGRVDALLTKKDQIPQENFHVGDRVKV
HCCEEEEHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCEEEE
YVTDVVLTPKGTRVFISRTAPDMLKRMFEKEIPEVFDGTVEIKSIARDAGDRAKVIVQSH
EEEEEEECCCCCEEEEECCCHHHHHHHHHHHCHHHHCCCHHHHHHHHCCCCCEEEEEECC
DENVDAIGTMVGTKGSRIQGIIRELAGEKMDIIEWSEDKATLVANALKPARIEQVLITAD
CCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEEC
GSSLAVVARDQLSLAIGKRGQNVRLAAHVTNSKIDIKAADEFNLDDYEFVGEDGEEIPTT
CCEEEEEEECCHHEEECCCCCCEEEEEEEECCEEEEEECCCCCCCCHHCCCCCCCCCCCC
EVNETLVVEEVVETPEVSESEE
CCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SKEMLNAFAMLEEQKGIKSEIVIEAIEQAITAAYKRQYGQAQNVKVIFDEKKGNFNVYS
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEE
TREVVDEVFDSRLEISLEDAHELNPHYEIGDKIMFEEKPKDFARTAAGAAKQVIMQKMRE
HHHHHHHHHCCCEEEEECHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
EERTIIYNEYSRYKDEIIQGTVEKVDARAVYVNLGRVDALLTKKDQIPQENFHVGDRVKV
HCCEEEEHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCEEEE
YVTDVVLTPKGTRVFISRTAPDMLKRMFEKEIPEVFDGTVEIKSIARDAGDRAKVIVQSH
EEEEEEECCCCCEEEEECCCHHHHHHHHHHHCHHHHCCCHHHHHHHHCCCCCEEEEEECC
DENVDAIGTMVGTKGSRIQGIIRELAGEKMDIIEWSEDKATLVANALKPARIEQVLITAD
CCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEEC
GSSLAVVARDQLSLAIGKRGQNVRLAAHVTNSKIDIKAADEFNLDDYEFVGEDGEEIPTT
CCEEEEEEECCHHEEECCCCCCEEEEEEEECCEEEEEECCCCCCCCHHCCCCCCCCCCCC
EVNETLVVEEVVETPEVSESEE
CCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]