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The map label for this gene is yhfA

Identifier: 15672725

GI number: 15672725

Start: 750333

End: 751001

Strand: Direct

Name: yhfA

Synonym: L150333

Alternate gene names: 15672725

Gene position: 750333-751001 (Clockwise)

Preceding gene: 15672721

Following gene: 15672726

Centisome position: 31.72

GC content: 34.23

Gene sequence:

>669_bases
ATGAAATTTAAAGCAGTGATTTTTGATATGGATGGTGTTCTTGTTGACACCGAACGTTATTATTTACAAAGAAGAGAAGA
ATTTTTTGGCGGTCATGGAATTTCTATTAGCCACTTGACGCCTAGTGATTTTATTGGTGGAAATATGAAAGATATTTGGC
CACGAATTTTGCAAGGGTCTTTTGATGAAGAAAAAGCGAGTGAATTGCAAGAAGACTATGAGCAATTCAAAGAAAATCAT
CCGATGCCTTATGCAGAACTTTTATTTTCTGATGTGCCTGATATTTTAGAATTTTTGCGTCAAAAAGAAGTAAAAATTGC
ACTTGCTTCAAGTTCGTCAATGTTTGATATCAAACAAATGTTAGATATTCATCAATTGAATTCTTATTTTGAAGTAGTGG
TTTCAGGAAATGACTTTAAACAAACGAAACCAAATCCAGAAATTTATCAATCAACAATGAAGAAATTGGGCGTAACACCT
TCTGAAACTTTAATTATTGAGGATTCTGAAAAAGGAATTCAAGCTGGAGAAGCAGCTGGTGCGACCGTTTGGGCAATTGA
AGACAAACGCTTTGGCATGAATCAAGAGAAGGCTGACTTAAAGGTAGATTCACTGACAGCTATTCAAAAATTACTGACAG
AATCATCACTAAAGATGATAAAGGCTTAA

Upstream 100 bases:

>100_bases
TCAAATTAGTTGTCTTGTCAACTAATTTCCGTGTAAAATAAAAATACTTTTATAATGAAGTCTATTGAACCATGCTTAGA
TACTGTGCTATAATGGTTCT

Downstream 100 bases:

>100_bases
TAAACATTTTAAAAGAAAAAATCACTGACAAATCGGTGATTCTTTAAGTTTTTTCTGTCCTATGTTAAAATAGAGGAATA
TAGGAGTAATATTATGAATT

Product: hypothetical protein

Products: Beta-D-Glucose 6- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MKFKAVIFDMDGVLVDTERYYLQRREEFFGGHGISISHLTPSDFIGGNMKDIWPRILQGSFDEEKASELQEDYEQFKENH
PMPYAELLFSDVPDILEFLRQKEVKIALASSSSMFDIKQMLDIHQLNSYFEVVVSGNDFKQTKPNPEIYQSTMKKLGVTP
SETLIIEDSEKGIQAGEAAGATVWAIEDKRFGMNQEKADLKVDSLTAIQKLLTESSLKMIKA

Sequences:

>Translated_222_residues
MKFKAVIFDMDGVLVDTERYYLQRREEFFGGHGISISHLTPSDFIGGNMKDIWPRILQGSFDEEKASELQEDYEQFKENH
PMPYAELLFSDVPDILEFLRQKEVKIALASSSSMFDIKQMLDIHQLNSYFEVVVSGNDFKQTKPNPEIYQSTMKKLGVTP
SETLIIEDSEKGIQAGEAAGATVWAIEDKRFGMNQEKADLKVDSLTAIQKLLTESSLKMIKA
>Mature_222_residues
MKFKAVIFDMDGVLVDTERYYLQRREEFFGGHGISISHLTPSDFIGGNMKDIWPRILQGSFDEEKASELQEDYEQFKENH
PMPYAELLFSDVPDILEFLRQKEVKIALASSSSMFDIKQMLDIHQLNSYFEVVVSGNDFKQTKPNPEIYQSTMKKLGVTP
SETLIIEDSEKGIQAGEAAGATVWAIEDKRFGMNQEKADLKVDSLTAIQKLLTESSLKMIKA

Specific function: Displays high phosphatase activity toward erythrose 4- phosphate, fructose 6-phosphate, 2-deoxyglucose 6-phosphate, and mannose 6-phosphate. May have a role in the intracellular metabolism of many phosphorylated carbohydrates [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787576, Length=193, Percent_Identity=28.4974093264249, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1789046, Length=192, Percent_Identity=26.5625, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788021, Length=215, Percent_Identity=27.4418604651163, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 5.4.2.6 [C]

Molecular weight: Translated: 25244; Mature: 25244

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKAVIFDMDGVLVDTERYYLQRREEFFGGHGISISHLTPSDFIGGNMKDIWPRILQGS
CCCEEEEEECCCEEECHHHHHHHHHHHHCCCCCEEEECCCCHHHCCCCHHHHHHHHHCCC
FDEEKASELQEDYEQFKENHPMPYAELLFSDVPDILEFLRQKEVKIALASSSSMFDIKQM
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHEEEEEECCCCHHHHHHH
LDIHQLNSYFEVVVSGNDFKQTKPNPEIYQSTMKKLGVTPSETLIIEDSEKGIQAGEAAG
HHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCC
ATVWAIEDKRFGMNQEKADLKVDSLTAIQKLLTESSLKMIKA
CEEEEEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFKAVIFDMDGVLVDTERYYLQRREEFFGGHGISISHLTPSDFIGGNMKDIWPRILQGS
CCCEEEEEECCCEEECHHHHHHHHHHHHCCCCCEEEECCCCHHHCCCCHHHHHHHHHCCC
FDEEKASELQEDYEQFKENHPMPYAELLFSDVPDILEFLRQKEVKIALASSSSMFDIKQM
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHEEEEEECCCCHHHHHHH
LDIHQLNSYFEVVVSGNDFKQTKPNPEIYQSTMKKLGVTPSETLIIEDSEKGIQAGEAAG
HHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCC
ATVWAIEDKRFGMNQEKADLKVDSLTAIQKLLTESSLKMIKA
CEEEEEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Beta-D-Glucose 1-Phosphate [C]

Specific reaction: Beta-D-Glucose 1-Phosphate = Beta-D-Glucose 6- Phosphate. [C]

General reaction: Group transfer (intramolecular phosphate group isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]