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The map label for this gene is ksgA

Identifier: 15672672

GI number: 15672672

Start: 689448

End: 690332

Strand: Direct

Name: ksgA

Synonym: L0363

Alternate gene names: 15672672

Gene position: 689448-690332 (Clockwise)

Preceding gene: 15672671

Following gene: 15672673

Centisome position: 29.14

GC content: 33.9

Gene sequence:

>885_bases
ATGACAGAAAACAACATAGACCGCATTTCCAATATTATCCGTACTCAGGACATTTTACGTCGCCATGATTTTAATTTTAA
AAAAAAATTTGGACAAAATTTCCTGACAGACCATAATATTCTGACAAAAATCACTCAAACTGCTGAACTTTCAAAAGAAG
TAAATGTCATTGAAATTGGTCCAGGTATTGGTTCTTTGACCCAATATTTATTGGAAGAAGCAGCTGAAGTGATGGCTTTT
GAAATTGACAAATCTTTGATTCCAATTTTAGAAGAAACGATGGCACCTTACGATAATTTTACCTTGGTTTCGGCAGATAT
TTTGAAAGTTGATTTACTTTCAGAAATTCAAAAATTTAAAAATCCAAATTTACCAATCAAAGTGGTTGCGAATTTACCTT
ATTACATCACAACACCGATTTTGATGCATTTGATTGAAAGCAAAATTCCATTTTCAGAATTTGTGGTGATGATGCAAAAA
GAAGTGGCAGACCGAATTGCTGCAAGTCCTAAAACGAAAGCTTACGGCTCATTATCAATTGCTGTTCAATATTATATGGA
AGCTAGTGTAGCCTTTATCGTACCTCGAACTGTCTTTATTCCAGCACCAAATGTTGATTCAGCTATTTTGAAAATGGTTC
GTCGTGAAGCACCATTGGTTGAAGTAGAAGATGAAGAATGGTTCTTTAAAACGATGCACAGCAGTTTTGTTCATCGTCGT
AAAACATTGATGAATAATTTACAAGCAGCTTTTGGTAAAGAAAGTAAACCTGAAATTGAAAAATTATTGGCACAAGCAGA
AATATCGCCAACAATTCGTGGTGAAGCCTTATCTATTGAAGAATTTGCTAAATTAGCTGATGCTCTACTTCCGCTTAAAA
AATAA

Upstream 100 bases:

>100_bases
ATGTCGCTGATAGAATACATCAATGGTTTCTGTCAGTAAAATTTACTGACAGAAACTTAATATCAGTTTTAAAATACATA
AAAAGTAAAAGAAAGAAAAA

Downstream 100 bases:

>100_bases
ATTAAAAGGAAACGTTAACTCATGTTAGCGTTTTTGTGTTATCATGTAAGAAAATGAAGAAATATTTTAAAGTTTAATTC
TTAATTTAAAGGAGAATAAA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTENNIDRISNIIRTQDILRRHDFNFKKKFGQNFLTDHNILTKITQTAELSKEVNVIEIGPGIGSLTQYLLEEAAEVMAF
EIDKSLIPILEETMAPYDNFTLVSADILKVDLLSEIQKFKNPNLPIKVVANLPYYITTPILMHLIESKIPFSEFVVMMQK
EVADRIAASPKTKAYGSLSIAVQYYMEASVAFIVPRTVFIPAPNVDSAILKMVRREAPLVEVEDEEWFFKTMHSSFVHRR
KTLMNNLQAAFGKESKPEIEKLLAQAEISPTIRGEALSIEEFAKLADALLPLKK

Sequences:

>Translated_294_residues
MTENNIDRISNIIRTQDILRRHDFNFKKKFGQNFLTDHNILTKITQTAELSKEVNVIEIGPGIGSLTQYLLEEAAEVMAF
EIDKSLIPILEETMAPYDNFTLVSADILKVDLLSEIQKFKNPNLPIKVVANLPYYITTPILMHLIESKIPFSEFVVMMQK
EVADRIAASPKTKAYGSLSIAVQYYMEASVAFIVPRTVFIPAPNVDSAILKMVRREAPLVEVEDEEWFFKTMHSSFVHRR
KTLMNNLQAAFGKESKPEIEKLLAQAEISPTIRGEALSIEEFAKLADALLPLKK
>Mature_293_residues
TENNIDRISNIIRTQDILRRHDFNFKKKFGQNFLTDHNILTKITQTAELSKEVNVIEIGPGIGSLTQYLLEEAAEVMAFE
IDKSLIPILEETMAPYDNFTLVSADILKVDLLSEIQKFKNPNLPIKVVANLPYYITTPILMHLIESKIPFSEFVVMMQKE
VADRIAASPKTKAYGSLSIAVQYYMEASVAFIVPRTVFIPAPNVDSAILKMVRREAPLVEVEDEEWFFKTMHSSFVHRRK
TLMNNLQAAFGKESKPEIEKLLAQAEISPTIRGEALSIEEFAKLADALLPLKK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=280, Percent_Identity=30.7142857142857, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI7657198, Length=223, Percent_Identity=30.4932735426009, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1786236, Length=263, Percent_Identity=31.1787072243346, Blast_Score=144, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI25141369, Length=298, Percent_Identity=28.8590604026846, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25146882, Length=224, Percent_Identity=33.4821428571429, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6324989, Length=207, Percent_Identity=28.5024154589372, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21358017, Length=245, Percent_Identity=31.8367346938775, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI21357273, Length=290, Percent_Identity=25.5172413793103, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_LACLA (Q9CHN8)

Other databases:

- EMBL:   AE005176
- PIR:   B86711
- RefSeq:   NP_266846.1
- ProteinModelPortal:   Q9CHN8
- SMR:   Q9CHN8
- GeneID:   1114315
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0363
- NMPDR:   fig|272623.1.peg.708
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- ProtClustDB:   PRK00274
- BioCyc:   LLAC272623:L0363-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 33345; Mature: 33214

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 33-33 BINDING 35-35 BINDING 60-60 BINDING 81-81 BINDING 106-106 BINDING 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENNIDRISNIIRTQDILRRHDFNFKKKFGQNFLTDHNILTKITQTAELSKEVNVIEIG
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCEEEEC
PGIGSLTQYLLEEAAEVMAFEIDKSLIPILEETMAPYDNFTLVSADILKVDLLSEIQKFK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHC
NPNLPIKVVANLPYYITTPILMHLIESKIPFSEFVVMMQKEVADRIAASPKTKAYGSLSI
CCCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
AVQYYMEASVAFIVPRTVFIPAPNVDSAILKMVRREAPLVEVEDEEWFFKTMHSSFVHRR
HHHHHHHHHHHEEECCEEEEECCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHH
KTLMNNLQAAFGKESKPEIEKLLAQAEISPTIRGEALSIEEFAKLADALLPLKK
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TENNIDRISNIIRTQDILRRHDFNFKKKFGQNFLTDHNILTKITQTAELSKEVNVIEIG
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCEEEEC
PGIGSLTQYLLEEAAEVMAFEIDKSLIPILEETMAPYDNFTLVSADILKVDLLSEIQKFK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHC
NPNLPIKVVANLPYYITTPILMHLIESKIPFSEFVVMMQKEVADRIAASPKTKAYGSLSI
CCCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
AVQYYMEASVAFIVPRTVFIPAPNVDSAILKMVRREAPLVEVEDEEWFFKTMHSSFVHRR
HHHHHHHHHHHEEECCEEEEECCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHH
KTLMNNLQAAFGKESKPEIEKLLAQAEISPTIRGEALSIEEFAKLADALLPLKK
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471