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The map label for this gene is ybiD

Identifier: 15672161

GI number: 15672161

Start: 184180

End: 185277

Strand: Direct

Name: ybiD

Synonym: L184159

Alternate gene names: 15672161

Gene position: 184180-185277 (Clockwise)

Preceding gene: 15672160

Following gene: 15672162

Centisome position: 7.79

GC content: 35.25

Gene sequence:

>1098_bases
ATGACAGAGAATATAATAACAGAAACGCGTCCTTCAATTTATGGATTGACACGTGACCAATTAATTGAATGGGCAATTGA
AAATGGAGAAAAGAAATTCCGAGCAACTCAGGTTTGGGATTGGCTTTACCGTAAACGTGTTCAATCTTTTGAAGAAATGA
GCAATTTATCAGCTGCTTTTATTGATAAACTTAATGAGCATTTTATTTTGAATCCATTAGAACAAGTTGTTGTTCAAGAA
TCTGCTGATGGAACAGTGAAATATCTATTTATGCTTCCTGACCAGATGATGATTGAAACAGTTTTGATGCGTCAATCTTA
TGGTTTGTCTGTTTGTGTTACAACTCAAGTAGGTTGTAATATGGGTTGTACTTTCTGTGCTTCTGGGATTTTGAAAAAAG
AACGTGATGTGACTGCTGGGGAAATTGTCAGCCAAATCATGCTCGTTCAAAAATACTTTGATGAACGTGGTTTAGATGAA
CGTGTTTCACACGTGGTAGTTATGGGAATTGGTGAACCTTTTGATAATTACGAACATCTAATGAACTTCTTGCGTGTTAT
TAATGATGATAATGGGCTGGCAATTGGGGCCCGTCACATCACAGTTTCGACTTGTGGATTTATGCCAGCAAAAATTAAAG
AATTTGCGCATGAAAATCTACAAATCAATTTAGCAATTTCACTTCATGCTCCAAATAATGAGTTGCGTACTTCATTGATG
CGTATTACTCGTAACGCACCACTTGAAAAACTTTTTGAAGCGATTGACTATTACACAGAAACAACCAATCGACGTGTGAC
TTACGAATATATTATGCTTTCTGGTGAAAATGACAGCCCAGAAATTGCTCAACAATTGGCTGACTTGATAAAACCTAGAA
ATAAATTGTCTTATGTTAACTTGATTCCATACAATCCAGTTGCAGAACATATTAAATATGAACGTTCAACAAAAGATAAT
ACGGCAAAATTTTATGATGTTTTGAAGAAAAATGGCATTAACTGTGTCGTTCGTCAAGAACATGGAACAGATATTGATGC
AGCTTGTGGACAATTACGTTCAAAACAAATTAAGAAAAATAAGGCCAAATTAGCCTAA

Upstream 100 bases:

>100_bases
TTTTCATAAATAAATGATAATAATTTAAATTGATTTTATGAGTGTATTCAAGGGATATGAAAAATTGCACGATTAAAATT
TGAGATACTTAAGGAAAATA

Downstream 100 bases:

>100_bases
TATAAAAACTGCTGACCAGGCAGTTTTTTTATTTCCACTTTTGTCAGTAAAATATTTCGGAAATAACCTATATTTAATAA
AAAAAACAAATATAATGAAT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTENIITETRPSIYGLTRDQLIEWAIENGEKKFRATQVWDWLYRKRVQSFEEMSNLSAAFIDKLNEHFILNPLEQVVVQE
SADGTVKYLFMLPDQMMIETVLMRQSYGLSVCVTTQVGCNMGCTFCASGILKKERDVTAGEIVSQIMLVQKYFDERGLDE
RVSHVVVMGIGEPFDNYEHLMNFLRVINDDNGLAIGARHITVSTCGFMPAKIKEFAHENLQINLAISLHAPNNELRTSLM
RITRNAPLEKLFEAIDYYTETTNRRVTYEYIMLSGENDSPEIAQQLADLIKPRNKLSYVNLIPYNPVAEHIKYERSTKDN
TAKFYDVLKKNGINCVVRQEHGTDIDAACGQLRSKQIKKNKAKLA

Sequences:

>Translated_365_residues
MTENIITETRPSIYGLTRDQLIEWAIENGEKKFRATQVWDWLYRKRVQSFEEMSNLSAAFIDKLNEHFILNPLEQVVVQE
SADGTVKYLFMLPDQMMIETVLMRQSYGLSVCVTTQVGCNMGCTFCASGILKKERDVTAGEIVSQIMLVQKYFDERGLDE
RVSHVVVMGIGEPFDNYEHLMNFLRVINDDNGLAIGARHITVSTCGFMPAKIKEFAHENLQINLAISLHAPNNELRTSLM
RITRNAPLEKLFEAIDYYTETTNRRVTYEYIMLSGENDSPEIAQQLADLIKPRNKLSYVNLIPYNPVAEHIKYERSTKDN
TAKFYDVLKKNGINCVVRQEHGTDIDAACGQLRSKQIKKNKAKLA
>Mature_364_residues
TENIITETRPSIYGLTRDQLIEWAIENGEKKFRATQVWDWLYRKRVQSFEEMSNLSAAFIDKLNEHFILNPLEQVVVQES
ADGTVKYLFMLPDQMMIETVLMRQSYGLSVCVTTQVGCNMGCTFCASGILKKERDVTAGEIVSQIMLVQKYFDERGLDER
VSHVVVMGIGEPFDNYEHLMNFLRVINDDNGLAIGARHITVSTCGFMPAKIKEFAHENLQINLAISLHAPNNELRTSLMR
ITRNAPLEKLFEAIDYYTETTNRRVTYEYIMLSGENDSPEIAQQLADLIKPRNKLSYVNLIPYNPVAEHIKYERSTKDNT
AKFYDVLKKNGINCVVRQEHGTDIDAACGQLRSKQIKKNKAKLA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=368, Percent_Identity=35.3260869565217, Blast_Score=216, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_LACLA (Q9CJ27)

Other databases:

- EMBL:   AE005176
- PIR:   C86647
- RefSeq:   NP_266335.1
- ProteinModelPortal:   Q9CJ27
- GeneID:   1113788
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L184159
- NMPDR:   fig|272623.1.peg.187
- HOGENOM:   HBG335345
- OMA:   VQKYFDE
- ProtClustDB:   PRK14455
- BioCyc:   LLAC272623:L184159-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41766; Mature: 41634

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENIITETRPSIYGLTRDQLIEWAIENGEKKFRATQVWDWLYRKRVQSFEEMSNLSAAF
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDKLNEHFILNPLEQVVVQESADGTVKYLFMLPDQMMIETVLMRQSYGLSVCVTTQVGCN
HHHHCCCEECCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCC
MGCTFCASGILKKERDVTAGEIVSQIMLVQKYFDERGLDERVSHVVVMGIGEPFDNYEHL
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCEEEEEECCCCCCCHHHH
MNFLRVINDDNGLAIGARHITVSTCGFMPAKIKEFAHENLQINLAISLHAPNNELRTSLM
HHHHHHHCCCCCEEEEEEEEEEEECCCCHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHH
RITRNAPLEKLFEAIDYYTETTNRRVTYEYIMLSGENDSPEIAQQLADLIKPRNKLSYVN
HHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEE
LIPYNPVAEHIKYERSTKDNTAKFYDVLKKNGINCVVRQEHGTDIDAACGQLRSKQIKKN
ECCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
KAKLA
HCCCC
>Mature Secondary Structure 
TENIITETRPSIYGLTRDQLIEWAIENGEKKFRATQVWDWLYRKRVQSFEEMSNLSAAF
CCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDKLNEHFILNPLEQVVVQESADGTVKYLFMLPDQMMIETVLMRQSYGLSVCVTTQVGCN
HHHHCCCEECCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCC
MGCTFCASGILKKERDVTAGEIVSQIMLVQKYFDERGLDERVSHVVVMGIGEPFDNYEHL
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCEEEEEECCCCCCCHHHH
MNFLRVINDDNGLAIGARHITVSTCGFMPAKIKEFAHENLQINLAISLHAPNNELRTSLM
HHHHHHHCCCCCEEEEEEEEEEEECCCCHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHH
RITRNAPLEKLFEAIDYYTETTNRRVTYEYIMLSGENDSPEIAQQLADLIKPRNKLSYVN
HHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEE
LIPYNPVAEHIKYERSTKDNTAKFYDVLKKNGINCVVRQEHGTDIDAACGQLRSKQIKKN
ECCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
KAKLA
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471