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The map label for this gene is dut

Identifier: 15672158

GI number: 15672158

Start: 181168

End: 181620

Strand: Direct

Name: dut

Synonym: L181168

Alternate gene names: 15672158

Gene position: 181168-181620 (Clockwise)

Preceding gene: 15672157

Following gene: 15672159

Centisome position: 7.66

GC content: 38.63

Gene sequence:

>453_bases
ATGAAAATTCGTGGATTTGAAGTGGTAACTAAATATAAAAATGCTGGAATTAATATACCAAAACGTTCAACTGAACATTC
AGCAGGTTATGACATTGAAGCAGCTGAAACCGTTAGTTTTGCGCCAGGGGAAATTAAATTAATTCCAACAGGCTTGAAGG
CCTATATGCAGGCAGGTGAAGTGCTTTACATGTATGACCGTTCATCAAATCCTCGTAAAAAAGGCTTGGTTTTAATCAAT
TCAGTAGGTGTTATTGACAAGGATTACTATAATAATCCTGATAATGAAGGGCATATGTTTATGCAGATGCGTAATTTCAC
TGATGAAGAAGTCGTAATTGAAAAAGGGGAGCGCGTGGTTCAGGGAGTCTTCATGCCTTTCTTGGTCGCTGATGGTGATG
AAAATCAAGAAAAAGAAGAACGGACTGGTGGATTTGGGTCAACAGGAGCTTAA

Upstream 100 bases:

>100_bases
TCAACATCTCATCGAAACAAATGCTGAAGAAGTCTAAATGAAAAGCGCTCTCTGAGTGCTTTTTTTATGATATAATAGAA
AAAAACAAATGAGGTAAGAG

Downstream 100 bases:

>100_bases
TTCAAATTAAAGTAATTTAAGAAGCGCAATAGCGCTTTTTATTTTTGCGAACAGAAGCTTTTAAAGCCTATTCATATTAC
CTGTGTTATAAATCTTATGT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MKIRGFEVVTKYKNAGINIPKRSTEHSAGYDIEAAETVSFAPGEIKLIPTGLKAYMQAGEVLYMYDRSSNPRKKGLVLIN
SVGVIDKDYYNNPDNEGHMFMQMRNFTDEEVVIEKGERVVQGVFMPFLVADGDENQEKEERTGGFGSTGA

Sequences:

>Translated_150_residues
MKIRGFEVVTKYKNAGINIPKRSTEHSAGYDIEAAETVSFAPGEIKLIPTGLKAYMQAGEVLYMYDRSSNPRKKGLVLIN
SVGVIDKDYYNNPDNEGHMFMQMRNFTDEEVVIEKGERVVQGVFMPFLVADGDENQEKEERTGGFGSTGA
>Mature_150_residues
MKIRGFEVVTKYKNAGINIPKRSTEHSAGYDIEAAETVSFAPGEIKLIPTGLKAYMQAGEVLYMYDRSSNPRKKGLVLIN
SVGVIDKDYYNNPDNEGHMFMQMRNFTDEEVVIEKGERVVQGVFMPFLVADGDENQEKEERTGGFGSTGA

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Escherichia coli, GI1790071, Length=140, Percent_Identity=35, Blast_Score=74, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_LACLA (Q9CJ30)

Other databases:

- EMBL:   AE005176
- PIR:   H86646
- RefSeq:   NP_266332.1
- ProteinModelPortal:   Q9CJ30
- SMR:   Q9CJ30
- GeneID:   1113785
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L181168
- NMPDR:   fig|272623.1.peg.184
- HOGENOM:   HBG436079
- OMA:   ERIAQGI
- ProtClustDB:   PRK13956
- BioCyc:   LLAC272623:L181168-MONOMER
- BRENDA:   3.6.1.23
- HAMAP:   MF_00116
- InterPro:   IPR008180

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16722; Mature: 16722

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: BINDING 80-80

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRGFEVVTKYKNAGINIPKRSTEHSAGYDIEAAETVSFAPGEIKLIPTGLKAYMQAGE
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCHHHHHCCCC
VLYMYDRSSNPRKKGLVLINSVGVIDKDYYNNPDNEGHMFMQMRNFTDEEVVIEKGERVV
EEEEEECCCCCCCCCEEEEECCCCEECCCCCCCCCCCEEEEEECCCCCCHHHHHCCHHHH
QGVFMPFLVADGDENQEKEERTGGFGSTGA
HHHHHEEEEECCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MKIRGFEVVTKYKNAGINIPKRSTEHSAGYDIEAAETVSFAPGEIKLIPTGLKAYMQAGE
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCHHHHHCCCC
VLYMYDRSSNPRKKGLVLINSVGVIDKDYYNNPDNEGHMFMQMRNFTDEEVVIEKGERVV
EEEEEECCCCCCCCCEEEEECCCCEECCCCCCCCCCCEEEEEECCCCCCHHHHHCCHHHH
QGVFMPFLVADGDENQEKEERTGGFGSTGA
HHHHHEEEEECCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471