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The map label for this gene is yagE

Identifier: 15672050

GI number: 15672050

Start: 69389

End: 70702

Strand: Direct

Name: yagE

Synonym: L69353

Alternate gene names: 15672050

Gene position: 69389-70702 (Clockwise)

Preceding gene: 15672049

Following gene: 15672051

Centisome position: 2.93

GC content: 35.54

Gene sequence:

>1314_bases
ATGAAAGAAAATAAATTATTATCACCGGCAGCCATTTTTCTGTTGGGTGTGAATTCCATTATTGGTTCAGGAATTTTCTT
ACTTTCTGGCAAAATTTATGAAGATGCAGGAATTTGGAGTTTACTGGCTATTTTATGTGCTGGTTTATCGATTTTTGTGA
TTGCTTTTGCTTATGCCAACATGAGTAAGATTTATTCAAAAAATGGTGGAGCTTTTGTTTATGCTAAAGAAACTTTTGGG
CGTTTTCCAGGCTTTATTGTGGGAATGGTGACATGGTTGCTAGGTACAGTGACACTAGCTACAGAGGTTTCGGTTTTACT
GACAGCAATGAAAATGATTCTTCCAAATCTTAATAGTCGGTTGATTGGCATCATTATTATTTTAGCCCTAGGAATCATCT
CCTATTTTGGGAGTTCAATTATTGCTGGTTTAGACAATCTGACTTCTTTTGTGAAAATTGTCTTAGTTCTTGTTTTTATT
TTTACTTGTCTATGGTTGGTACGTGGTCTTAATTTTTCAAGTCCAAATTTACTGACAGCTTCGTCAGCAAATTTAGGTGG
CTTTAAAGGATTTCTGTCAGCTTACGGTACTGTGTTCTTTTTCTTCACCGGCTTTTCATTTCTACCGGTGAATGCTGAAA
AAATGAAAAATCCAGCAAAAAATTTACCAAAAATGATGTCCCTTGTCATGTTAACCTGTTTGGCTGTTTATCTGATTATT
CAAACCATTACCATTGGGGCATTGGGGCCAGAACTGACTAAAACACTTGTGCCTGCGGCAACAGCTTTTAGTAAAATCGT
GGGTAGCATTGGAATTCCGCTCTTTGTTGTCGGAATATGTTTTTCAATTTTTGGTGTTATTGTTGCGACCACTTTTAATA
CACCAACAATTTTAGCCAGTTTGGCTCAGGCTCATGAAGATGTGCCTATGTTTGTTTCTAAAAATAATCGCTTTGGAACC
CCCACTTGGGCAATTATTTTGACGACAGTTTGTGGAATAGCTCTCTTTTTGAGTGGAAATTATGTTTTTCTGAGTGGCCT
GACTGTCTTTATGAGTTTTGTTCAATATTTATCAACTGGACTTGCTAACATTAAGAAAAAATTCCTTTTGATTGGAATTG
GTTCGGTTCTCTTTTCATTGGTTTTACTGACAAGCTTTACTTTATCCGTCCTAATTTTAGGTTTAGGAATCTTGATATTA
CTTGTTATGATTTATTTTGTCGTTAAATTAGATGATGATGAACGTGAAAAATGGCTCAAAGAACACCAAGAAAAGTCAAA
AGGAGCAGATGTCAATACTGTTAAACGTTTGTAA

Upstream 100 bases:

>100_bases
AACTGGTGAAATAATGACTAAAAATGAGGCCTTTCTGATATAATTTAACTAACAATAAATTTAACAAAGTCTTAACTTAA
TTAAAATAAGTGAGGTTTCA

Downstream 100 bases:

>100_bases
GTAATTTTTAGATATTTTATTTTACTGAAGAAAGCTGTCAGTAAAATAAGGAGAAAAATATGACAAAAAAAGAATATACA
TTTTGGTTGTTGAGCAGTAT

Product: amino acid permease

Products: D-glycine [Cytoplasm]; D-alanine [Cytoplasm]; Proton [Cytoplasm]; D-serine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MKENKLLSPAAIFLLGVNSIIGSGIFLLSGKIYEDAGIWSLLAILCAGLSIFVIAFAYANMSKIYSKNGGAFVYAKETFG
RFPGFIVGMVTWLLGTVTLATEVSVLLTAMKMILPNLNSRLIGIIIILALGIISYFGSSIIAGLDNLTSFVKIVLVLVFI
FTCLWLVRGLNFSSPNLLTASSANLGGFKGFLSAYGTVFFFFTGFSFLPVNAEKMKNPAKNLPKMMSLVMLTCLAVYLII
QTITIGALGPELTKTLVPAATAFSKIVGSIGIPLFVVGICFSIFGVIVATTFNTPTILASLAQAHEDVPMFVSKNNRFGT
PTWAIILTTVCGIALFLSGNYVFLSGLTVFMSFVQYLSTGLANIKKKFLLIGIGSVLFSLVLLTSFTLSVLILGLGILIL
LVMIYFVVKLDDDEREKWLKEHQEKSKGADVNTVKRL

Sequences:

>Translated_437_residues
MKENKLLSPAAIFLLGVNSIIGSGIFLLSGKIYEDAGIWSLLAILCAGLSIFVIAFAYANMSKIYSKNGGAFVYAKETFG
RFPGFIVGMVTWLLGTVTLATEVSVLLTAMKMILPNLNSRLIGIIIILALGIISYFGSSIIAGLDNLTSFVKIVLVLVFI
FTCLWLVRGLNFSSPNLLTASSANLGGFKGFLSAYGTVFFFFTGFSFLPVNAEKMKNPAKNLPKMMSLVMLTCLAVYLII
QTITIGALGPELTKTLVPAATAFSKIVGSIGIPLFVVGICFSIFGVIVATTFNTPTILASLAQAHEDVPMFVSKNNRFGT
PTWAIILTTVCGIALFLSGNYVFLSGLTVFMSFVQYLSTGLANIKKKFLLIGIGSVLFSLVLLTSFTLSVLILGLGILIL
LVMIYFVVKLDDDEREKWLKEHQEKSKGADVNTVKRL
>Mature_437_residues
MKENKLLSPAAIFLLGVNSIIGSGIFLLSGKIYEDAGIWSLLAILCAGLSIFVIAFAYANMSKIYSKNGGAFVYAKETFG
RFPGFIVGMVTWLLGTVTLATEVSVLLTAMKMILPNLNSRLIGIIIILALGIISYFGSSIIAGLDNLTSFVKIVLVLVFI
FTCLWLVRGLNFSSPNLLTASSANLGGFKGFLSAYGTVFFFFTGFSFLPVNAEKMKNPAKNLPKMMSLVMLTCLAVYLII
QTITIGALGPELTKTLVPAATAFSKIVGSIGIPLFVVGICFSIFGVIVATTFNTPTILASLAQAHEDVPMFVSKNNRFGT
PTWAIILTTVCGIALFLSGNYVFLSGLTVFMSFVQYLSTGLANIKKKFLLIGIGSVLFSLVLLTSFTLSVLILGLGILIL
LVMIYFVVKLDDDEREKWLKEHQEKSKGADVNTVKRL

Specific function: Probable amino-acid or metabolite transport protein [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Homo sapiens, GI181337167, Length=335, Percent_Identity=23.2835820895522, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790653, Length=343, Percent_Identity=22.7405247813411, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789017, Length=413, Percent_Identity=22.7602905569007, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17540018, Length=365, Percent_Identity=23.8356164383562, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281366235, Length=328, Percent_Identity=24.390243902439, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI116007820, Length=328, Percent_Identity=24.390243902439, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI116007818, Length=328, Percent_Identity=24.390243902439, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI85725152, Length=328, Percent_Identity=24.390243902439, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI45550968, Length=339, Percent_Identity=23.598820058997, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19921172, Length=339, Percent_Identity=23.598820058997, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24667468, Length=245, Percent_Identity=25.3061224489796, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 47193; Mature: 47193

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENKLLSPAAIFLLGVNSIIGSGIFLLSGKIYEDAGIWSLLAILCAGLSIFVIAFAYAN
CCCCCCCCHHHHHHHHHHHHHHCCHHHEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MSKIYSKNGGAFVYAKETFGRFPGFIVGMVTWLLGTVTLATEVSVLLTAMKMILPNLNSR
HHHHHHCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LIGIIIILALGIISYFGSSIIAGLDNLTSFVKIVLVLVFIFTCLWLVRGLNFSSPNLLTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
SSANLGGFKGFLSAYGTVFFFFTGFSFLPVNAEKMKNPAKNLPKMMSLVMLTCLAVYLII
CCCCCCHHHHHHHHHHHHHHHHHCCCEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
QTITIGALGPELTKTLVPAATAFSKIVGSIGIPLFVVGICFSIFGVIVATTFNTPTILAS
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LAQAHEDVPMFVSKNNRFGTPTWAIILTTVCGIALFLSGNYVFLSGLTVFMSFVQYLSTG
HHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LANIKKKFLLIGIGSVLFSLVLLTSFTLSVLILGLGILILLVMIYFVVKLDDDEREKWLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EHQEKSKGADVNTVKRL
HHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MKENKLLSPAAIFLLGVNSIIGSGIFLLSGKIYEDAGIWSLLAILCAGLSIFVIAFAYAN
CCCCCCCCHHHHHHHHHHHHHHCCHHHEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MSKIYSKNGGAFVYAKETFGRFPGFIVGMVTWLLGTVTLATEVSVLLTAMKMILPNLNSR
HHHHHHCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LIGIIIILALGIISYFGSSIIAGLDNLTSFVKIVLVLVFIFTCLWLVRGLNFSSPNLLTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
SSANLGGFKGFLSAYGTVFFFFTGFSFLPVNAEKMKNPAKNLPKMMSLVMLTCLAVYLII
CCCCCCHHHHHHHHHHHHHHHHHCCCEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
QTITIGALGPELTKTLVPAATAFSKIVGSIGIPLFVVGICFSIFGVIVATTFNTPTILAS
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LAQAHEDVPMFVSKNNRFGTPTWAIILTTVCGIALFLSGNYVFLSGLTVFMSFVQYLSTG
HHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LANIKKKFLLIGIGSVLFSLVLLTSFTLSVLILGLGILILLVMIYFVVKLDDDEREKWLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EHQEKSKGADVNTVKRL
HHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-glycine [Periplasm]; D-alanine [Periplasm]; Proton [Periplasm]; D-serine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + D-glycine [Periplasm] = Proton [Cytoplasm] + D-glycine [Cytoplasm] Proton [Periplasm] + D-alanine [Periplasm] = Proton [Cytoplasm] + D-alanine [Cytoplasm] Proton [Periplasm] + D-serine [Periplasm] = Proton [Cytoplasm] + D-serine [Cyto

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]