Click here to switch to the map view.

The map label for this gene is mtlF

Identifier: 15672005

GI number: 15672005

Start: 32907

End: 33338

Strand: Direct

Name: mtlF

Synonym: L32907

Alternate gene names: 15672005

Gene position: 32907-33338 (Clockwise)

Preceding gene: 15672004

Following gene: 15672006

Centisome position: 1.39

GC content: 32.87

Gene sequence:

>432_bases
ATGGAAATTCAAAAAAGTCTGATTAAAATTAATCAAAACTTTTCAAATAAAGAAGAGGCCATAAAATATTGTGGAAGTTT
GTTGGTTGATGGAGGTTATGTCAAACCTAACTATATCTCAGCGATGATTGAACGAGATAGAGAATTGTCAGTCTATATGG
GAAACTTTATTGCAATTCCTCATGGAACTGATGAATCAAAAAAAGAAGTTTTAAAAACAGGAGTAGCCATTGTTCAAGTT
CCTCGTGGTGTAAATTTTGGAACAGTAGAAAATCCTAAGATAGCAACAATGCTATTTGGAATTGCGGGAATTAAAGAGGA
GCATCTTGAGCTGATTCAAAAGATTTCAATTTTTTGCGCGGACGTTGACAATGTTGTAAAACTTGCTGATGCTCAAACAA
TCGAAGAAATTGCAAAATACTTTGAAAATTAA

Upstream 100 bases:

>100_bases
GTGAGTTACTAAGAGAGATAATTATCGAAGCTGTTAAACATTATGATGACTAGGTTTACATAAGTTGTAAACCTAGTAAA
CCAAGAAAGGTAGTTTTTAA

Downstream 100 bases:

>100_bases
TTAAAAAATGGCACAGCTTTTAGTGCCATTTTTTATATTTACCAGCATAATGTAAAAGCTTACAATTACTTATATAGAGG
GATTTTAGAGAATTCTATTT

Product: mannitol-specific PTS system IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Mtl; EIII-Mtl; PTS system mannitol-specific EIIA component [H]

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MEIQKSLIKINQNFSNKEEAIKYCGSLLVDGGYVKPNYISAMIERDRELSVYMGNFIAIPHGTDESKKEVLKTGVAIVQV
PRGVNFGTVENPKIATMLFGIAGIKEEHLELIQKISIFCADVDNVVKLADAQTIEEIAKYFEN

Sequences:

>Translated_143_residues
MEIQKSLIKINQNFSNKEEAIKYCGSLLVDGGYVKPNYISAMIERDRELSVYMGNFIAIPHGTDESKKEVLKTGVAIVQV
PRGVNFGTVENPKIATMLFGIAGIKEEHLELIQKISIFCADVDNVVKLADAQTIEEIAKYFEN
>Mature_143_residues
MEIQKSLIKINQNFSNKEEAIKYCGSLLVDGGYVKPNYISAMIERDRELSVYMGNFIAIPHGTDESKKEVLKTGVAIVQV
PRGVNFGTVENPKIATMLFGIAGIKEEHLELIQKISIFCADVDNVVKLADAQTIEEIAKYFEN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790027, Length=130, Percent_Identity=40.7692307692308, Blast_Score=106, Evalue=7e-25,
Organism=Escherichia coli, GI1788494, Length=122, Percent_Identity=38.5245901639344, Blast_Score=98, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 15940; Mature: 15940

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIQKSLIKINQNFSNKEEAIKYCGSLLVDGGYVKPNYISAMIERDRELSVYMGNFIAIP
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCEEEEC
HGTDESKKEVLKTGVAIVQVPRGVNFGTVENPKIATMLFGIAGIKEEHLELIQKISIFCA
CCCCHHHHHHHHHCHHEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DVDNVVKLADAQTIEEIAKYFEN
CHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEIQKSLIKINQNFSNKEEAIKYCGSLLVDGGYVKPNYISAMIERDRELSVYMGNFIAIP
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCEEEEC
HGTDESKKEVLKTGVAIVQVPRGVNFGTVENPKIATMLFGIAGIKEEHLELIQKISIFCA
CCCCHHHHHHHHHCHHEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DVDNVVKLADAQTIEEIAKYFEN
CHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1322373; 12397186 [H]