Click here to switch to the map view.

The map label for this gene is lipA

Identifier: 15835461

GI number: 15835461

Start: 983540

End: 984466

Strand: Direct

Name: lipA

Synonym: TC0847

Alternate gene names: 15835461

Gene position: 983540-984466 (Clockwise)

Preceding gene: 15835460

Following gene: 15835462

Centisome position: 91.67

GC content: 43.37

Gene sequence:

>927_bases
ATGAGTAATTCCCCAGAATCCTCAACTCCCAAGCAGTCCATCCCAGCACGATTCCCCAAATGGTTGCGACAAAAACTTCC
TCTAGGGAAAGTATTTTCTCGAACTGATGGCACTATAAAAAATAAGGGGCTCCCCACTGTTTGTGAAGAAGCCTCTTGTC
CCAATCGCACGCATTGTTGGTCCAGACATACCGCTACATACCTTGCATTGGGTGATGCATGTACACGTCGCTGTGGCTTT
TGTGATATTGATTTCACTAAAAAACCTCTTCCCCCAGATCCTCAAGAAGGAGAGAAAATCGCTGCTTCCGCAAAAGCATT
GGGCCTAAAACATATCGTGATCACCATGGTTTCTCGCGATGATTTGGAGGATGGCGGAGCAGATGCTCTAGCTCGTATCA
TTACAACCTTACATATAGAACTTCCAGAAGCTACTATTGAAGTTCTAGCCTCTGATTTTGAAGGGAACATTGATGCTTTA
CACCATCTACTTGATGCACGTATTGCCATCTATAACCATAATGTAGAAACTGTAGAACGACTATCTCCCCTTGTTCGTCA
TAAAGCAACCTACCGACGGTCTCTGATGATGTTAGAGCAGGCTGCACAATACCTCCCAGACCTTATGATTAAGTCTGGTA
TAATGGTAGGATTAGGAGAACAGGAAAGCGAAATCAAGCAAACGTTAAAAGATCTTGCAGATCATGGCGTTAAGATAGTT
ACTATAGGACAATACCTTCGTCCTTCGCGAAGACATATTCCTGTGAAAAGCTATGTTTCTCCAGAAACATTTGATTATTA
TCGATCCGTAGGGGAGGCTTTGGGTCTTTTCATTTATGCAGGACCTTTCGTTCGTTCTAGCTTTAATGCAGATGCTGTTT
TTGAAGCTATGAGTCAACGAGAGCGCCTGTCCGCTTCTATACAATAG

Upstream 100 bases:

>100_bases
TTTATGAAACCATCCATGCACATCCAACCTTAGCAGAAGTTTGGGCAGAAAGTGCACTATTGGCCGTTGATACCCCGTTA
CATATGCCCCCTACTAGAAA

Downstream 100 bases:

>100_bases
CTAAAAACCAACTCATAACCCACTAAGCTTAAACATCCTTTAACATAAAAAATGTTCCCGATTGGCACTAATCTCCCCAT
TTGCTATGGTGAGTAAAAAG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSNSPESSTPKQSIPARFPKWLRQKLPLGKVFSRTDGTIKNKGLPTVCEEASCPNRTHCWSRHTATYLALGDACTRRCGF
CDIDFTKKPLPPDPQEGEKIAASAKALGLKHIVITMVSRDDLEDGGADALARIITTLHIELPEATIEVLASDFEGNIDAL
HHLLDARIAIYNHNVETVERLSPLVRHKATYRRSLMMLEQAAQYLPDLMIKSGIMVGLGEQESEIKQTLKDLADHGVKIV
TIGQYLRPSRRHIPVKSYVSPETFDYYRSVGEALGLFIYAGPFVRSSFNADAVFEAMSQRERLSASIQ

Sequences:

>Translated_308_residues
MSNSPESSTPKQSIPARFPKWLRQKLPLGKVFSRTDGTIKNKGLPTVCEEASCPNRTHCWSRHTATYLALGDACTRRCGF
CDIDFTKKPLPPDPQEGEKIAASAKALGLKHIVITMVSRDDLEDGGADALARIITTLHIELPEATIEVLASDFEGNIDAL
HHLLDARIAIYNHNVETVERLSPLVRHKATYRRSLMMLEQAAQYLPDLMIKSGIMVGLGEQESEIKQTLKDLADHGVKIV
TIGQYLRPSRRHIPVKSYVSPETFDYYRSVGEALGLFIYAGPFVRSSFNADAVFEAMSQRERLSASIQ
>Mature_307_residues
SNSPESSTPKQSIPARFPKWLRQKLPLGKVFSRTDGTIKNKGLPTVCEEASCPNRTHCWSRHTATYLALGDACTRRCGFC
DIDFTKKPLPPDPQEGEKIAASAKALGLKHIVITMVSRDDLEDGGADALARIITTLHIELPEATIEVLASDFEGNIDALH
HLLDARIAIYNHNVETVERLSPLVRHKATYRRSLMMLEQAAQYLPDLMIKSGIMVGLGEQESEIKQTLKDLADHGVKIVT
IGQYLRPSRRHIPVKSYVSPETFDYYRSVGEALGLFIYAGPFVRSSFNADAVFEAMSQRERLSASIQ

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=39.2982456140351, Blast_Score=234, Evalue=8e-62,
Organism=Homo sapiens, GI37577164, Length=245, Percent_Identity=38.7755102040816, Blast_Score=197, Evalue=9e-51,
Organism=Escherichia coli, GI1786846, Length=287, Percent_Identity=44.5993031358885, Blast_Score=237, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI32564533, Length=296, Percent_Identity=41.8918918918919, Blast_Score=235, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=285, Percent_Identity=43.859649122807, Blast_Score=259, Evalue=3e-70,
Organism=Drosophila melanogaster, GI221513272, Length=285, Percent_Identity=41.0526315789474, Blast_Score=245, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_CHLMU (Q9PJI2)

Other databases:

- EMBL:   AE002160
- PIR:   C81658
- RefSeq:   NP_297220.1
- ProteinModelPortal:   Q9PJI2
- GeneID:   1246215
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0847
- TIGR:   TC_0847
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   Q9PJI2
- ProtClustDB:   PRK05481
- BioCyc:   CMUR243161:TC_0847-MONOMER
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 34279; Mature: 34147

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSPESSTPKQSIPARFPKWLRQKLPLGKVFSRTDGTIKNKGLPTVCEEASCPNRTHCW
CCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCHHH
SRHTATYLALGDACTRRCGFCDIDFTKKPLPPDPQEGEKIAASAKALGLKHIVITMVSRD
HHHCHHHEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
DLEDGGADALARIITTLHIELPEATIEVLASDFEGNIDALHHLLDARIAIYNHNVETVER
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCHHHHHH
LSPLVRHKATYRRSLMMLEQAAQYLPDLMIKSGIMVGLGEQESEIKQTLKDLADHGVKIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCEEEE
TIGQYLRPSRRHIPVKSYVSPETFDYYRSVGEALGLFIYAGPFVRSSFNADAVFEAMSQR
EECHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHH
ERLSASIQ
HHHHCCCC
>Mature Secondary Structure 
SNSPESSTPKQSIPARFPKWLRQKLPLGKVFSRTDGTIKNKGLPTVCEEASCPNRTHCW
CCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCHHH
SRHTATYLALGDACTRRCGFCDIDFTKKPLPPDPQEGEKIAASAKALGLKHIVITMVSRD
HHHCHHHEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
DLEDGGADALARIITTLHIELPEATIEVLASDFEGNIDALHHLLDARIAIYNHNVETVER
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCHHHHHH
LSPLVRHKATYRRSLMMLEQAAQYLPDLMIKSGIMVGLGEQESEIKQTLKDLADHGVKIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCEEEE
TIGQYLRPSRRHIPVKSYVSPETFDYYRSVGEALGLFIYAGPFVRSSFNADAVFEAMSQR
EECHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHH
ERLSASIQ
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935