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Identifier: 15835379

GI number: 15835379

Start: 907934

End: 910024

Strand: Reverse

Name: Not Available

Synonym: TC0765

Alternate gene names: 15835379

Gene position: 910024-907934 (Counterclockwise)

Preceding gene: 15835380

Following gene: 15835378

Centisome position: 84.82

GC content: 41.46

Gene sequence:

>2091_bases
ATGATAGATAAAATTATACGAACGATATTGGTTCTATCCTTATTCCTACTGTATTGGTCCTCAGATTTACTTGAAAAAGA
TATTAAAGCGATTAAAAGAGAGCTGAAGGCGTTGCACGAAGATGTTCTTGAGCTAGTTAGGATTTCGAATCAGCAAAGCA
ATTTTGTGCAAACCATGAATTCTCAGGCTTCTCCTGAGCCATGTGTGTACGCAGATTGCGGGGATCCTTCTTTACCCAAT
TTATTATGTGAGGACCCTTACGTTGAAAAAGTAGTTCCTTCGTTATTAAAAGAAGGGTTTGTTCCGAAAGGAGTCCTTCG
TACAGCTCAAGTGGGACGACCTGACAATTTGAGCCCGTTTAATGGCTTTGTCAATATCGTGCGATTTTATGAGTTATGTG
TTCCTTCTTTGGCCACGGAACATATTGGTAAGTATGAAGAGTTTGCTCCTAGTCTTGCTGTAAAGATAGAAGAACATTAT
GCACAGGACGGATCAGGGGATAAAGAATTCCACATTTATCTACGGCCAAATATGTTTTGGGAACCAATCGATCCAACGCT
CTTCCCTAAAAATGTGACTTTGGCAGAGTCTTTTTTAAGACCCCACCCAGTAACTGCTCATGATGTGAAGTTTTACTATG
ACGTAGTCATGAATCCTTATGTAGCAGAAATGCGTGCTGTGGCCATGAGATCATATTTTGAAGATGTGGTTTCTGTTCGT
GTAGAAAATGATTTGAAATTAGTTGTGCGTTGGCGAGCTCATACTGTGAACAACGAACAGGGGGAAGAGGAAAAGAAAGT
ATTGTATTCGGCCTTTATCAATACATTGGCTCTTCAACCTCTTCCATGCTTTGTATACAAGTATTTCGCGAATGGGGAAA
AAATTGTTCACGATGATTCTGATCCGGATACCTATCGGAAAGATTCCGTATGGGCTCAAAATTTTTCTTCGCACTGGGCT
TATAACTATATAGTCAGCTGTGGAGCTTTCCGATTTGCAGGGATGGATGATGAGAAGATCGCTTTGGTGAGAAATCCTAA
TTATTATAATCCTTTTGCTGCTCTTGTAGAGAAGCGGTATGTCTATATGAAAGACAGCACAGACTCACTCTTCCAAGATT
TCAAAGCGGGTAAAGTGGATATTGCTTATTTCCCTCCTAATCAAGTGGACAACCTTGCTAGCTTTATGAAGACTGCAGCC
TATAAAGAACAGGCAGCTAAAGGAGGGGCTATCATAGAAAAAAATTCTTCGGATCGGTCCTATTCTTATATTGGATGGAA
TTGTCTTTCTCTTTTCTTTAAAGATCGAGCAGTTCGACAGGCGATGAATATGCTAATTGATCGGGATCGTATCATTGAGC
AGTGCTTAGATGGACGAGGATCCTCAATAACTGGGCCCTTTTCTCTTTGTTCTCCATCGTATAATAGAAGTGTAGAAGGC
TGGCAGTACTCTCCAGAAGAGGCGTCTCGTAAATTGGAAGAAGAAGGCTGGGTTGATGCTGATGGAGATGGAATTCGAGA
GAAAGTGATAGATGGGGTAGTAGTGCCCTTCCGATTCCGACTGTGCTACTATGTAAAAAGTGTGACAGCAAGAACTATTG
CTGAATATGTGGCCACGGTATGCAAAGAAATCGGCATCGAATGTTGTTTACTTGGGCTAGATATGGCGGATTATTCACAA
GCTCTTGAAGAGAAGAATTTTGATGCCATCCTTTCTGGATGGAGTTTAGGAACTCCTCCGGAGGATCCGCGCTCCTTGTG
GCACTCAGAAGGGGCTTTAGAGAAGGGCTCAGCAAATGCTGTAGGATTCTGTAATAAAGAAGCTGACCGTATTATAGAAC
AGCTGAGTTATGAGTACGATCCGAATAAACGGCAGGAGCTATATCATCGTTTCCATGAGGTGATTCATGAAGAATCTCCT
TATGCTTTTCTGTATTCAAGACAATACTCCTTGGTTCATAAGAAGTATGTAAAAAATATTTTCATTCCAACAGAACATCC
GGATTGGATTCCTGGGGCCCAGGATGAAACAGTGAATCTGTCGATGATGTGGGTAGATAGAGAGGAGGGACAATGCTCCG
CTATATCTTAA

Upstream 100 bases:

>100_bases
CCAGGTACTAGAATTCAAAATTCGAATTGATAAAAGTATTCGAGACAAGGTTTGTCTCTTGGCTTTTGGGTGCGTTTTCA
ACGAATAGAGGAGACAATGC

Downstream 100 bases:

>100_bases
AACGGTTATGCCTGATTCCTTTGACTCTTTTTGCGATTATTTCTGTAAATTTTGTTATTCTTAATGCTGCTCCAGGAGAC
CTTCTTGAGGAACATAGTGT

Product: peptide ABC transporter, periplasmic peptide-binding protein, putative

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 696; Mature: 696

Protein sequence:

>696_residues
MIDKIIRTILVLSLFLLYWSSDLLEKDIKAIKRELKALHEDVLELVRISNQQSNFVQTMNSQASPEPCVYADCGDPSLPN
LLCEDPYVEKVVPSLLKEGFVPKGVLRTAQVGRPDNLSPFNGFVNIVRFYELCVPSLATEHIGKYEEFAPSLAVKIEEHY
AQDGSGDKEFHIYLRPNMFWEPIDPTLFPKNVTLAESFLRPHPVTAHDVKFYYDVVMNPYVAEMRAVAMRSYFEDVVSVR
VENDLKLVVRWRAHTVNNEQGEEEKKVLYSAFINTLALQPLPCFVYKYFANGEKIVHDDSDPDTYRKDSVWAQNFSSHWA
YNYIVSCGAFRFAGMDDEKIALVRNPNYYNPFAALVEKRYVYMKDSTDSLFQDFKAGKVDIAYFPPNQVDNLASFMKTAA
YKEQAAKGGAIIEKNSSDRSYSYIGWNCLSLFFKDRAVRQAMNMLIDRDRIIEQCLDGRGSSITGPFSLCSPSYNRSVEG
WQYSPEEASRKLEEEGWVDADGDGIREKVIDGVVVPFRFRLCYYVKSVTARTIAEYVATVCKEIGIECCLLGLDMADYSQ
ALEEKNFDAILSGWSLGTPPEDPRSLWHSEGALEKGSANAVGFCNKEADRIIEQLSYEYDPNKRQELYHRFHEVIHEESP
YAFLYSRQYSLVHKKYVKNIFIPTEHPDWIPGAQDETVNLSMMWVDREEGQCSAIS

Sequences:

>Translated_696_residues
MIDKIIRTILVLSLFLLYWSSDLLEKDIKAIKRELKALHEDVLELVRISNQQSNFVQTMNSQASPEPCVYADCGDPSLPN
LLCEDPYVEKVVPSLLKEGFVPKGVLRTAQVGRPDNLSPFNGFVNIVRFYELCVPSLATEHIGKYEEFAPSLAVKIEEHY
AQDGSGDKEFHIYLRPNMFWEPIDPTLFPKNVTLAESFLRPHPVTAHDVKFYYDVVMNPYVAEMRAVAMRSYFEDVVSVR
VENDLKLVVRWRAHTVNNEQGEEEKKVLYSAFINTLALQPLPCFVYKYFANGEKIVHDDSDPDTYRKDSVWAQNFSSHWA
YNYIVSCGAFRFAGMDDEKIALVRNPNYYNPFAALVEKRYVYMKDSTDSLFQDFKAGKVDIAYFPPNQVDNLASFMKTAA
YKEQAAKGGAIIEKNSSDRSYSYIGWNCLSLFFKDRAVRQAMNMLIDRDRIIEQCLDGRGSSITGPFSLCSPSYNRSVEG
WQYSPEEASRKLEEEGWVDADGDGIREKVIDGVVVPFRFRLCYYVKSVTARTIAEYVATVCKEIGIECCLLGLDMADYSQ
ALEEKNFDAILSGWSLGTPPEDPRSLWHSEGALEKGSANAVGFCNKEADRIIEQLSYEYDPNKRQELYHRFHEVIHEESP
YAFLYSRQYSLVHKKYVKNIFIPTEHPDWIPGAQDETVNLSMMWVDREEGQCSAIS
>Mature_696_residues
MIDKIIRTILVLSLFLLYWSSDLLEKDIKAIKRELKALHEDVLELVRISNQQSNFVQTMNSQASPEPCVYADCGDPSLPN
LLCEDPYVEKVVPSLLKEGFVPKGVLRTAQVGRPDNLSPFNGFVNIVRFYELCVPSLATEHIGKYEEFAPSLAVKIEEHY
AQDGSGDKEFHIYLRPNMFWEPIDPTLFPKNVTLAESFLRPHPVTAHDVKFYYDVVMNPYVAEMRAVAMRSYFEDVVSVR
VENDLKLVVRWRAHTVNNEQGEEEKKVLYSAFINTLALQPLPCFVYKYFANGEKIVHDDSDPDTYRKDSVWAQNFSSHWA
YNYIVSCGAFRFAGMDDEKIALVRNPNYYNPFAALVEKRYVYMKDSTDSLFQDFKAGKVDIAYFPPNQVDNLASFMKTAA
YKEQAAKGGAIIEKNSSDRSYSYIGWNCLSLFFKDRAVRQAMNMLIDRDRIIEQCLDGRGSSITGPFSLCSPSYNRSVEG
WQYSPEEASRKLEEEGWVDADGDGIREKVIDGVVVPFRFRLCYYVKSVTARTIAEYVATVCKEIGIECCLLGLDMADYSQ
ALEEKNFDAILSGWSLGTPPEDPRSLWHSEGALEKGSANAVGFCNKEADRIIEQLSYEYDPNKRQELYHRFHEVIHEESP
YAFLYSRQYSLVHKKYVKNIFIPTEHPDWIPGAQDETVNLSMMWVDREEGQCSAIS

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=327, Percent_Identity=23.8532110091743, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 79685; Mature: 79685

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDKIIRTILVLSLFLLYWSSDLLEKDIKAIKRELKALHEDVLELVRISNQQSNFVQTMN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
SQASPEPCVYADCGDPSLPNLLCEDPYVEKVVPSLLKEGFVPKGVLRTAQVGRPDNLSPF
CCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHH
NGFVNIVRFYELCVPSLATEHIGKYEEFAPSLAVKIEEHYAQDGSGDKEFHIYLRPNMFW
HHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHEEEEHHHHCCCCCCCCEEEEEECCCCCC
EPIDPTLFPKNVTLAESFLRPHPVTAHDVKFYYDVVMNPYVAEMRAVAMRSYFEDVVSVR
CCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
VENDLKLVVRWRAHTVNNEQGEEEKKVLYSAFINTLALQPLPCFVYKYFANGEKIVHDDS
CCCCEEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCC
DPDTYRKDSVWAQNFSSHWAYNYIVSCGAFRFAGMDDEKIALVRNPNYYNPFAALVEKRY
CCCCHHHHHHHHHHHHCCHHHHHHHHHCCEEECCCCCCCEEEEECCCCCCHHHHHHHHHE
VYMKDSTDSLFQDFKAGKVDIAYFPPNQVDNLASFMKTAAYKEQAAKGGAIIEKNSSDRS
EEEECCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
YSYIGWNCLSLFFKDRAVRQAMNMLIDRDRIIEQCLDGRGSSITGPFSLCSPSYNRSVEG
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCC
WQYSPEEASRKLEEEGWVDADGDGIREKVIDGVVVPFRFRLCYYVKSVTARTIAEYVATV
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
CKEIGIECCLLGLDMADYSQALEEKNFDAILSGWSLGTPPEDPRSLWHSEGALEKGSANA
HHHCCCCEEEECCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCC
VGFCNKEADRIIEQLSYEYDPNKRQELYHRFHEVIHEESPYAFLYSRQYSLVHKKYVKNI
CCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHC
FIPTEHPDWIPGAQDETVNLSMMWVDREEGQCSAIS
CCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MIDKIIRTILVLSLFLLYWSSDLLEKDIKAIKRELKALHEDVLELVRISNQQSNFVQTMN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
SQASPEPCVYADCGDPSLPNLLCEDPYVEKVVPSLLKEGFVPKGVLRTAQVGRPDNLSPF
CCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHH
NGFVNIVRFYELCVPSLATEHIGKYEEFAPSLAVKIEEHYAQDGSGDKEFHIYLRPNMFW
HHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHEEEEHHHHCCCCCCCCEEEEEECCCCCC
EPIDPTLFPKNVTLAESFLRPHPVTAHDVKFYYDVVMNPYVAEMRAVAMRSYFEDVVSVR
CCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
VENDLKLVVRWRAHTVNNEQGEEEKKVLYSAFINTLALQPLPCFVYKYFANGEKIVHDDS
CCCCEEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCC
DPDTYRKDSVWAQNFSSHWAYNYIVSCGAFRFAGMDDEKIALVRNPNYYNPFAALVEKRY
CCCCHHHHHHHHHHHHCCHHHHHHHHHCCEEECCCCCCCEEEEECCCCCCHHHHHHHHHE
VYMKDSTDSLFQDFKAGKVDIAYFPPNQVDNLASFMKTAAYKEQAAKGGAIIEKNSSDRS
EEEECCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
YSYIGWNCLSLFFKDRAVRQAMNMLIDRDRIIEQCLDGRGSSITGPFSLCSPSYNRSVEG
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCC
WQYSPEEASRKLEEEGWVDADGDGIREKVIDGVVVPFRFRLCYYVKSVTARTIAEYVATV
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
CKEIGIECCLLGLDMADYSQALEEKNFDAILSGWSLGTPPEDPRSLWHSEGALEKGSANA
HHHCCCCEEEECCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCC
VGFCNKEADRIIEQLSYEYDPNKRQELYHRFHEVIHEESPYAFLYSRQYSLVHKKYVKNI
CCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHC
FIPTEHPDWIPGAQDETVNLSMMWVDREEGQCSAIS
CCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]