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The map label for this gene is recJ

Identifier: 15835347

GI number: 15835347

Start: 869998

End: 871746

Strand: Reverse

Name: recJ

Synonym: TC0732

Alternate gene names: 15835347

Gene position: 871746-869998 (Counterclockwise)

Preceding gene: 15835348

Following gene: 15835346

Centisome position: 81.25

GC content: 39.62

Gene sequence:

>1749_bases
ATGGAAAAAGACAAATTATCTGCACTCGGACAAAAATGGACGTATCCTAAGCATAATGAGGAGTTTCTGAAGAAGATTAT
AAAAGAATTGTATCTTCATCCTGTAATTGCTCAAGTTCTTGTATCGCGAGGGTTTCAATCTGTCCAAGAGATCCGAGATT
TTTTGTATCCGCAATTCCCATCGTTACATCCAGCCTCTTCGTTTTTGGATATGGAAAAAGCTGTCGCTCGTCTCCTTAAA
TCTAAGGAAAACAATGAGCATGTTATGATCTATGGCGATGGGGATGTAGACGGCATAACAGGTGTGACTTTACTCGTAGA
ATTTTTACAAATGCTCGGAGTAAAGACGAGCTATTGTTGTTCCGGTACTTTGTTTAAACAACACGGAGAAATTACCTCTT
TGATTTCTCAGATGCTACAAGATGGCGTTTCTCTCTTAATTACTGTGGATTGTGGAATTACTGCAGGAAGGGAAGTTCGT
TCCATTAACAAACAGGGTATAGATGTTATTGTCACAGATCATCATACGCCTACAGGGAAACTTCCCCATTGTATTGCCAC
GTTGAATCCGAAATTAGATAAAAATTCTTATCCAAATAAGGAGCTGACCGGGGTTGGGGTAGCTTTTAAGTTAGTATGTG
CAACTTATGAAGAACTGATTAAGCAGGATGCTTCTTGGAAAGATAAAATAGATCTGTTGCATTTTTTAGATTTAGTGAGT
TTAGGAACAATTGCAGATGTTGGCAGACTTTCCGGGGAGAATCGTATTTTAGTTTCCTATGGAATTAAAGAAATCACAAA
AGGAAAGCGTTTAGGCTTAAAAAAGCTTTGTTCTTTGTCAGGGGTTGATAAATCGGATATTTCTTCAACAAATTTAGGAA
TTAGAATTACCCCTAAATTAAATAGCTTAGGAAGATTAGCAGATTCTAGTCAGGGCGTTAAGTTATTATTATCTCAAGAT
CCTCGAACTGTGGGAACAATCGTTTCGGAGCTTGCTATTGTGAATCAGGAGCGTCAGAGAATAGAGGCTGAAGTGCTCCG
TGATGTAGAAAGAATTTTGGCAGCAAATCCTAAGCTAACTGCTCAATCTGCCATTGTATTGGCATCTCCTTATTGGCATT
CTCGAGTCATCCCGATTATTTCTGCTCGTTTGGCACGTACCTATAACAAACCCGTAGCAATTATTGCTCTTCAAGATGGT
ATAGGAAAAGGTTCTTTACGTACGATAGGGGCGTTCCCTTTATTAGGAGTGCTTCGTAAATGCGAATCGTTTTTTCTATC
CTATGGAGGACATGATTTTGCAGCAGGGCTTATGATCAAAGAGGAGCAGATAGAAGGGTTCAGGAAAAAATTCATCCATT
TAGTGAATTCCTCCTTAAGGAAAGACGAGGCTACGTTAACCTTGCCTTTGGATGTATGCATGGATTTCTCTCAAATTAGC
CAAGATCTGATCTCTTCTATGGAGCTTCTGGAGCCTTTTGGTAAGGGGAATCCGTCTCCAGTTTTTTATACAAGGGCCAT
ACAAGTACGTTATCCAAAGCTATTATCTGGGAATCACGTAAAATTATATTTAAATAGTGGGGAAAGAAATTTGGAGGGCA
CGGCTTTTGGTCAGGGAGATAAGATTAGTTTGCTCAAGGCTAATTGGAATATGCCTCTAGATATCGCTTATACACTGCGT
GTTATGAAGCGGTCTGTTCATGGGGCGGTTCGTCTTCTTATTCAGGACTTTCGTATACAGGCTCCTTGA

Upstream 100 bases:

>100_bases
GGCGTATGTAATCCTGGAATTATGGGATTATTGCTCCTTTCCTCCTGAAGGAGGAAAGGAGCTTTTTTTTAAAAGAGCTC
CTCTCCTGGGATGAGAGTAT

Downstream 100 bases:

>100_bases
TCGCTTGTATCTCCTGGCGGTCATTGTACCATTCTTATCGAGATCGGGAGGAGGTATTTTACCCCTGTCATCTTCAACTC
TAGCTTAACTATCTTATTTT

Product: single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MEKDKLSALGQKWTYPKHNEEFLKKIIKELYLHPVIAQVLVSRGFQSVQEIRDFLYPQFPSLHPASSFLDMEKAVARLLK
SKENNEHVMIYGDGDVDGITGVTLLVEFLQMLGVKTSYCCSGTLFKQHGEITSLISQMLQDGVSLLITVDCGITAGREVR
SINKQGIDVIVTDHHTPTGKLPHCIATLNPKLDKNSYPNKELTGVGVAFKLVCATYEELIKQDASWKDKIDLLHFLDLVS
LGTIADVGRLSGENRILVSYGIKEITKGKRLGLKKLCSLSGVDKSDISSTNLGIRITPKLNSLGRLADSSQGVKLLLSQD
PRTVGTIVSELAIVNQERQRIEAEVLRDVERILAANPKLTAQSAIVLASPYWHSRVIPIISARLARTYNKPVAIIALQDG
IGKGSLRTIGAFPLLGVLRKCESFFLSYGGHDFAAGLMIKEEQIEGFRKKFIHLVNSSLRKDEATLTLPLDVCMDFSQIS
QDLISSMELLEPFGKGNPSPVFYTRAIQVRYPKLLSGNHVKLYLNSGERNLEGTAFGQGDKISLLKANWNMPLDIAYTLR
VMKRSVHGAVRLLIQDFRIQAP

Sequences:

>Translated_582_residues
MEKDKLSALGQKWTYPKHNEEFLKKIIKELYLHPVIAQVLVSRGFQSVQEIRDFLYPQFPSLHPASSFLDMEKAVARLLK
SKENNEHVMIYGDGDVDGITGVTLLVEFLQMLGVKTSYCCSGTLFKQHGEITSLISQMLQDGVSLLITVDCGITAGREVR
SINKQGIDVIVTDHHTPTGKLPHCIATLNPKLDKNSYPNKELTGVGVAFKLVCATYEELIKQDASWKDKIDLLHFLDLVS
LGTIADVGRLSGENRILVSYGIKEITKGKRLGLKKLCSLSGVDKSDISSTNLGIRITPKLNSLGRLADSSQGVKLLLSQD
PRTVGTIVSELAIVNQERQRIEAEVLRDVERILAANPKLTAQSAIVLASPYWHSRVIPIISARLARTYNKPVAIIALQDG
IGKGSLRTIGAFPLLGVLRKCESFFLSYGGHDFAAGLMIKEEQIEGFRKKFIHLVNSSLRKDEATLTLPLDVCMDFSQIS
QDLISSMELLEPFGKGNPSPVFYTRAIQVRYPKLLSGNHVKLYLNSGERNLEGTAFGQGDKISLLKANWNMPLDIAYTLR
VMKRSVHGAVRLLIQDFRIQAP
>Mature_582_residues
MEKDKLSALGQKWTYPKHNEEFLKKIIKELYLHPVIAQVLVSRGFQSVQEIRDFLYPQFPSLHPASSFLDMEKAVARLLK
SKENNEHVMIYGDGDVDGITGVTLLVEFLQMLGVKTSYCCSGTLFKQHGEITSLISQMLQDGVSLLITVDCGITAGREVR
SINKQGIDVIVTDHHTPTGKLPHCIATLNPKLDKNSYPNKELTGVGVAFKLVCATYEELIKQDASWKDKIDLLHFLDLVS
LGTIADVGRLSGENRILVSYGIKEITKGKRLGLKKLCSLSGVDKSDISSTNLGIRITPKLNSLGRLADSSQGVKLLLSQD
PRTVGTIVSELAIVNQERQRIEAEVLRDVERILAANPKLTAQSAIVLASPYWHSRVIPIISARLARTYNKPVAIIALQDG
IGKGSLRTIGAFPLLGVLRKCESFFLSYGGHDFAAGLMIKEEQIEGFRKKFIHLVNSSLRKDEATLTLPLDVCMDFSQIS
QDLISSMELLEPFGKGNPSPVFYTRAIQVRYPKLLSGNHVKLYLNSGERNLEGTAFGQGDKISLLKANWNMPLDIAYTLR
VMKRSVHGAVRLLIQDFRIQAP

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=532, Percent_Identity=32.3308270676692, Blast_Score=229, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 64520; Mature: 64520

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDKLSALGQKWTYPKHNEEFLKKIIKELYLHPVIAQVLVSRGFQSVQEIRDFLYPQFP
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCC
SLHPASSFLDMEKAVARLLKSKENNEHVMIYGDGDVDGITGVTLLVEFLQMLGVKTSYCC
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHCC
SGTLFKQHGEITSLISQMLQDGVSLLITVDCGITAGREVRSINKQGIDVIVTDHHTPTGK
CCCHHHHCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHCCCCCEEEEECCCCCCCC
LPHCIATLNPKLDKNSYPNKELTGVGVAFKLVCATYEELIKQDASWKDKIDLLHFLDLVS
CCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LGTIADVGRLSGENRILVSYGIKEITKGKRLGLKKLCSLSGVDKSDISSTNLGIRITPKL
HHHHHHHHHCCCCCEEEEEECHHHHHCCHHCCHHHHHHHCCCCCCCCCCCCCCEEECCCC
NSLGRLADSSQGVKLLLSQDPRTVGTIVSELAIVNQERQRIEAEVLRDVERILAANPKLT
CHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AQSAIVLASPYWHSRVIPIISARLARTYNKPVAIIALQDGIGKGSLRTIGAFPLLGVLRK
CCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
CESFFLSYGGHDFAAGLMIKEEQIEGFRKKFIHLVNSSLRKDEATLTLPLDVCMDFSQIS
HHHHHHHCCCCCHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCHHHHH
QDLISSMELLEPFGKGNPSPVFYTRAIQVRYPKLLSGNHVKLYLNSGERNLEGTAFGQGD
HHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCHHCCCCEEEEEEECCCCCCCCCCCCCCC
KISLLKANWNMPLDIAYTLRVMKRSVHGAVRLLIQDFRIQAP
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKDKLSALGQKWTYPKHNEEFLKKIIKELYLHPVIAQVLVSRGFQSVQEIRDFLYPQFP
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCC
SLHPASSFLDMEKAVARLLKSKENNEHVMIYGDGDVDGITGVTLLVEFLQMLGVKTSYCC
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHCC
SGTLFKQHGEITSLISQMLQDGVSLLITVDCGITAGREVRSINKQGIDVIVTDHHTPTGK
CCCHHHHCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHCCCCCEEEEECCCCCCCC
LPHCIATLNPKLDKNSYPNKELTGVGVAFKLVCATYEELIKQDASWKDKIDLLHFLDLVS
CCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LGTIADVGRLSGENRILVSYGIKEITKGKRLGLKKLCSLSGVDKSDISSTNLGIRITPKL
HHHHHHHHHCCCCCEEEEEECHHHHHCCHHCCHHHHHHHCCCCCCCCCCCCCCEEECCCC
NSLGRLADSSQGVKLLLSQDPRTVGTIVSELAIVNQERQRIEAEVLRDVERILAANPKLT
CHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AQSAIVLASPYWHSRVIPIISARLARTYNKPVAIIALQDGIGKGSLRTIGAFPLLGVLRK
CCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
CESFFLSYGGHDFAAGLMIKEEQIEGFRKKFIHLVNSSLRKDEATLTLPLDVCMDFSQIS
HHHHHHHCCCCCHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCHHHHH
QDLISSMELLEPFGKGNPSPVFYTRAIQVRYPKLLSGNHVKLYLNSGERNLEGTAFGQGD
HHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCHHCCCCEEEEEEECCCCCCCCCCCCCCC
KISLLKANWNMPLDIAYTLRVMKRSVHGAVRLLIQDFRIQAP
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]