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The map label for this gene is dapF

Identifier: 15835329

GI number: 15835329

Start: 852241

End: 853050

Strand: Reverse

Name: dapF

Synonym: TC0714

Alternate gene names: 15835329

Gene position: 853050-852241 (Counterclockwise)

Preceding gene: 15835330

Following gene: 15835328

Centisome position: 79.51

GC content: 39.75

Gene sequence:

>810_bases
ATGGGATCCTTTTCTCCTTTAATGACTTATAGGTATCATCTATATTCTGGAACTGGTAATAGTTTTATTTTAGGGGAGTT
CATACCTCCCCTCCAACATATTGTGTTTCTATGCCAGAAAGAGAAGGTCGATGGATTTTTATGTGTAGAACCTTCTGAGA
TAGCAGATGCTAAGCTAACGATTTTTAATAGCGATGGATCTGAAGCTTCTATGTGTGGCAATGGATTGCGGTGCGTAATG
GCTCATGTAGCTCAGAGTTTGGGCTTAGAGGATGTTTCCATTGAAACCGTTCGCGGGGTCTATCAAGGTAAGTTTTTTTC
TATGGATCGAGTATTAGTTGATATGACATTACTTGATTGGAAGAAGACTAAGAAAACATTAACACATGTATTGCCTGGCA
TGCCGGAAGAGGTATTCTTTATTGATACGGGAGTCCCGCATGTTGTTGTCTTTGTTCCTGATGTGAATAAGGTTCCTGTA
CAAGAATGGGGGGCTTTCCTTCGTTATCATGAAGATTTTAGGCCTAATGGAGTCAATGTAGACTTTGTTCAGACAAAGAA
AGAAGACACGCTACTTGTTTATACTTATGAGCGGGGATGCGAGCGAGAAACTCTTTCTTGTGGGACAGGAATGTTAGCGA
GTGCTTTAGTTGCTGCAGATGTTTTTTCGTTAGAACAAGATTTTTCTCTGTTAGTATGTTCTCGGAGTGGAAACATTGTT
AAGATTTTTTCTGAAAACGGTAAGGTTTTTTTAGAAGGGCCTGTAACCCTTTTGAATTGTAGTGAAAATATTGGGGAGTT
TGCCCCCTAA

Upstream 100 bases:

>100_bases
TATGTTGAAGCAACAGGGCAGTCTCGAGAGGTGATAGAGAAAGCTATTGATCGAGATATGTGGATGAGTGCCAATGAAGC
TATGGAGTTTGGTCTATTAG

Downstream 100 bases:

>100_bases
TCTAGATGTATTAGACAAATTTGAGGATAAAACAAGAGAGGGTTGCTATCTAACAGAGCATTCCCTATACTGGAGCCTAT
GACAACATATCCTGTACCTC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MGSFSPLMTYRYHLYSGTGNSFILGEFIPPLQHIVFLCQKEKVDGFLCVEPSEIADAKLTIFNSDGSEASMCGNGLRCVM
AHVAQSLGLEDVSIETVRGVYQGKFFSMDRVLVDMTLLDWKKTKKTLTHVLPGMPEEVFFIDTGVPHVVVFVPDVNKVPV
QEWGAFLRYHEDFRPNGVNVDFVQTKKEDTLLVYTYERGCERETLSCGTGMLASALVAADVFSLEQDFSLLVCSRSGNIV
KIFSENGKVFLEGPVTLLNCSENIGEFAP

Sequences:

>Translated_269_residues
MGSFSPLMTYRYHLYSGTGNSFILGEFIPPLQHIVFLCQKEKVDGFLCVEPSEIADAKLTIFNSDGSEASMCGNGLRCVM
AHVAQSLGLEDVSIETVRGVYQGKFFSMDRVLVDMTLLDWKKTKKTLTHVLPGMPEEVFFIDTGVPHVVVFVPDVNKVPV
QEWGAFLRYHEDFRPNGVNVDFVQTKKEDTLLVYTYERGCERETLSCGTGMLASALVAADVFSLEQDFSLLVCSRSGNIV
KIFSENGKVFLEGPVTLLNCSENIGEFAP
>Mature_268_residues
GSFSPLMTYRYHLYSGTGNSFILGEFIPPLQHIVFLCQKEKVDGFLCVEPSEIADAKLTIFNSDGSEASMCGNGLRCVMA
HVAQSLGLEDVSIETVRGVYQGKFFSMDRVLVDMTLLDWKKTKKTLTHVLPGMPEEVFFIDTGVPHVVVFVPDVNKVPVQ
EWGAFLRYHEDFRPNGVNVDFVQTKKEDTLLVYTYERGCERETLSCGTGMLASALVAADVFSLEQDFSLLVCSRSGNIVK
IFSENGKVFLEGPVTLLNCSENIGEFAP

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=189, Percent_Identity=31.7460317460317, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_CHLMU (Q9PJW2)

Other databases:

- EMBL:   AE002160
- RefSeq:   NP_297088.1
- ProteinModelPortal:   Q9PJW2
- SMR:   Q9PJW2
- GeneID:   1246077
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0714
- TIGR:   TC_0714
- HOGENOM:   HBG399442
- OMA:   NCASVIA
- ProtClustDB:   PRK00450
- BioCyc:   CMUR243161:TC_0714-MONOMER
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 29865; Mature: 29733

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 72-72 ACT_SITE 207-207

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSFSPLMTYRYHLYSGTGNSFILGEFIPPLQHIVFLCQKEKVDGFLCVEPSEIADAKLT
CCCCCCCEEEEEEEEECCCCCEEEHHHCCHHHHHHHHHHHCCCCEEEEECCHHHCCEEEE
IFNSDGSEASMCGNGLRCVMAHVAQSLGLEDVSIETVRGVYQGKFFSMDRVLVDMTLLDW
EEECCCCCHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEHHHHEEEHHHHHH
KKTKKTLTHVLPGMPEEVFFIDTGVPHVVVFVPDVNKVPVQEWGAFLRYHEDFRPNGVNV
HHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEE
DFVQTKKEDTLLVYTYERGCERETLSCGTGMLASALVAADVFSLEQDFSLLVCSRSGNIV
EEEEECCCCEEEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCEE
KIFSENGKVFLEGPVTLLNCSENIGEFAP
EEEECCCEEEEECCEEEEECCCCCCCCCC
>Mature Secondary Structure 
GSFSPLMTYRYHLYSGTGNSFILGEFIPPLQHIVFLCQKEKVDGFLCVEPSEIADAKLT
CCCCCCEEEEEEEEECCCCCEEEHHHCCHHHHHHHHHHHCCCCEEEEECCHHHCCEEEE
IFNSDGSEASMCGNGLRCVMAHVAQSLGLEDVSIETVRGVYQGKFFSMDRVLVDMTLLDW
EEECCCCCHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEHHHHEEEHHHHHH
KKTKKTLTHVLPGMPEEVFFIDTGVPHVVVFVPDVNKVPVQEWGAFLRYHEDFRPNGVNV
HHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEE
DFVQTKKEDTLLVYTYERGCERETLSCGTGMLASALVAADVFSLEQDFSLLVCSRSGNIV
EEEEECCCCEEEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCEE
KIFSENGKVFLEGPVTLLNCSENIGEFAP
EEEECCCEEEEECCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935