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Identifier: 15835305

GI number: 15835305

Start: 820257

End: 821618

Strand: Direct

Name: Not Available

Synonym: TC0690

Alternate gene names: 15835305

Gene position: 820257-821618 (Clockwise)

Preceding gene: 15835304

Following gene: 15835306

Centisome position: 76.45

GC content: 42.29

Gene sequence:

>1362_bases
ATGAACACGTTTCTATCTTTTTTGACAGCTTTTGATGACTTTATTTGGTCATATGTAGCGTTTATTTTAATCCTTTCGCT
CGGAGTCGTGTTCACGTACAAATCGAATTTCGCACAATTCACGCAGCTTCCTTCATTTTTTAAACTGTTCTATCGATTCT
CGAAGGAGTCTTCGTCTACACAGAAAGATCTGAAGAAAGGTGTTCATCCATTAAAAGTTTTTTTCGCTTCCGCTGGAGGA
AATATTGGTATAGGGAACGTTGTTGGGGTAGTCACTGCTGCTAGTGTCGGAGGCCCTGGAGCGCTCTTTTGGGTCTGGAT
AGCAGGGATTCTTGGATCTATCGTTAAATACTCTGAAGTTTATCTAGGAATTAAATTCCGCCAATCCGACGCACATGGTG
TGTATCACGGAGGACCCATGTTCTTCCTTGATAAGGCCTATAAAACCCGTATTGTCTCCATCATTGTGGCCATTCTTCTC
TGTATCTATGGAGTGGAGATTTACCAATTTTCGGTGATTACAAACACTTTAACCTCCTGCTGGAACGTTCCTAAAATTAT
TTCTATCGCCTCCCTTCTTTTCTTAGTTATTTTTGCTGTGCAAGGAGGTCTGCAACGAATAGGGAAAATCTGTTCCTTAG
TTTTACCCTTCTTCATGTTGATTTACTGCGGAATGGCTTTTTACATCTTAGCTCATGAAATCCATACCCTTCCAACGCTT
TTCTCAAGCATCTTCCGTTCCGCCTTTACAGGTCATGGCGCTATTGGAGGATTTGCTGGCTGCACCGTTGCATCCACTAT
TCGCCAAGGGCTCTCTAGAGCTGCCTATTCGGGAGACATCGGTATTGGGTTTGATTCCATTATCCAAAGCGAGACATCGG
CTACAAATCCCCAGACACAAGCTCAACTCAGCATTGTCGGAGTTGTTGTAGATAATCTTGTCTGTACATTAAGTCTTCTC
ATCGTCCTCGCTTCTGGTGTTTGGCACAGAGTAGGCCTGGAGGGATCTGAGGTTGTGGAACAAGCCCTTTCCTCATATTT
CCCCTATATTCGTATATTCCTTCCTGCATTCCTTTTTGCGACAGGATATACAACGATTATTTCTTACTTCCTTGTAGGGA
AGAAATGCGCGAATTTTATTTGCGGTAACAAAGGATCTAAATTCTATACCTGTTATGGTATTTTAGCTTTGCCAGCTTTC
TGCTTTCTACCACAAGATACGGCATTACTCGTTATGTCTGTCTCAGGGGCATTGTTGCTATGCATCAATCTATTCGGAGT
TTTTTTAATGCGAAAAGAACTCGTCTTCCCCAAAAAAGCTGAGGAAACTCTTGAAGTATCTGGATCCTCTTTCTCTTCTT
AG

Upstream 100 bases:

>100_bases
AATAAAAATTGTTCTCTAAAAGATCCTCTACCAAACAAAAAAGAAAAAGAGGATAATATAGGGCAATTTTCAGAAGAAAA
TGATTTAAATTACATATTTT

Downstream 100 bases:

>100_bases
TGAGAAAGAGGTATAAAGCTGTTGCCGATCAAAGTGACGTTCCTTATATATGGTTCTTCTGACACCATAAAAAATTTTTA
GAAACTTATAGGTAGTTTTT

Product: sodium/alanine symporter family protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MNTFLSFLTAFDDFIWSYVAFILILSLGVVFTYKSNFAQFTQLPSFFKLFYRFSKESSSTQKDLKKGVHPLKVFFASAGG
NIGIGNVVGVVTAASVGGPGALFWVWIAGILGSIVKYSEVYLGIKFRQSDAHGVYHGGPMFFLDKAYKTRIVSIIVAILL
CIYGVEIYQFSVITNTLTSCWNVPKIISIASLLFLVIFAVQGGLQRIGKICSLVLPFFMLIYCGMAFYILAHEIHTLPTL
FSSIFRSAFTGHGAIGGFAGCTVASTIRQGLSRAAYSGDIGIGFDSIIQSETSATNPQTQAQLSIVGVVVDNLVCTLSLL
IVLASGVWHRVGLEGSEVVEQALSSYFPYIRIFLPAFLFATGYTTIISYFLVGKKCANFICGNKGSKFYTCYGILALPAF
CFLPQDTALLVMSVSGALLLCINLFGVFLMRKELVFPKKAEETLEVSGSSFSS

Sequences:

>Translated_453_residues
MNTFLSFLTAFDDFIWSYVAFILILSLGVVFTYKSNFAQFTQLPSFFKLFYRFSKESSSTQKDLKKGVHPLKVFFASAGG
NIGIGNVVGVVTAASVGGPGALFWVWIAGILGSIVKYSEVYLGIKFRQSDAHGVYHGGPMFFLDKAYKTRIVSIIVAILL
CIYGVEIYQFSVITNTLTSCWNVPKIISIASLLFLVIFAVQGGLQRIGKICSLVLPFFMLIYCGMAFYILAHEIHTLPTL
FSSIFRSAFTGHGAIGGFAGCTVASTIRQGLSRAAYSGDIGIGFDSIIQSETSATNPQTQAQLSIVGVVVDNLVCTLSLL
IVLASGVWHRVGLEGSEVVEQALSSYFPYIRIFLPAFLFATGYTTIISYFLVGKKCANFICGNKGSKFYTCYGILALPAF
CFLPQDTALLVMSVSGALLLCINLFGVFLMRKELVFPKKAEETLEVSGSSFSS
>Mature_453_residues
MNTFLSFLTAFDDFIWSYVAFILILSLGVVFTYKSNFAQFTQLPSFFKLFYRFSKESSSTQKDLKKGVHPLKVFFASAGG
NIGIGNVVGVVTAASVGGPGALFWVWIAGILGSIVKYSEVYLGIKFRQSDAHGVYHGGPMFFLDKAYKTRIVSIIVAILL
CIYGVEIYQFSVITNTLTSCWNVPKIISIASLLFLVIFAVQGGLQRIGKICSLVLPFFMLIYCGMAFYILAHEIHTLPTL
FSSIFRSAFTGHGAIGGFAGCTVASTIRQGLSRAAYSGDIGIGFDSIIQSETSATNPQTQAQLSIVGVVVDNLVCTLSLL
IVLASGVWHRVGLEGSEVVEQALSSYFPYIRIFLPAFLFATGYTTIISYFLVGKKCANFICGNKGSKFYTCYGILALPAF
CFLPQDTALLVMSVSGALLLCINLFGVFLMRKELVFPKKAEETLEVSGSSFSS

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=357, Percent_Identity=27.4509803921569, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 49307; Mature: 49307

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]