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The map label for this gene is pyk

Identifier: 15835226

GI number: 15835226

Start: 728454

End: 729899

Strand: Direct

Name: pyk

Synonym: TC0609

Alternate gene names: 15835226

Gene position: 728454-729899 (Clockwise)

Preceding gene: 15835225

Following gene: 15835227

Centisome position: 67.89

GC content: 42.81

Gene sequence:

>1446_bases
ATGATCGCTAGAACAAAAATTATTTGTACAATAGGCCCTGCAACAAACACCCCAGAAATGCTTGAAAAACTTCTTGATGC
GGGGATGAATGTAGCGCGTCTTAACTTCAGTCATGGTACCCACGAAAGCCACGGCCGGACCATTGCTATTCTTAAGGAAC
TACGCGAAAAGCGCCAAGTCCCTTTAGCTATTATGTTGGATACAAAAGGACCAGAAATTCGTTTAGGCCAAGTAGAATCT
CCTATAAAAGTGAAGCCAGGAGACCGTCTCACTTTAACCAGTAAAGAAATTTTGGGATCCAAAGAAGCTGGAGTCACTCT
TTATCCTAGCTGCGTGTTCCCTTTCGTTCGCGAACGCGCTCCCGTCCTGATTGATGATGGATATATCCAAGCCGTAGTTG
TCAATGCTCAAGAGCATCTCATTGAGATAGAATTTCAGAATTCAGGAGAAATCAAGTCTAATAAATCACTTAGCATCAAA
GATATAGACGTAGCCCTCCCCTTCATGACAGAGAAGGATATCACGGATCTAAAATTCGGGGTCGAACAAGAACTTGACCT
TATCGCAGCATCTTTTGTCCGATGTAACGAAGACATCGATAGCATGCGTAAAGTTTTAGAAAACTTCGGCCGGCCAAATA
TGCCGATCATTGCCAAAATAGAAAATCATTTAGGGGTACAAAATTTCCAAGAAATAGCCAAAGCTTCTGATGGAATTATG
ATCGCACGAGGAGATCTCGGCATCGAATTATCTATCGTTGAAGTCCCTGCCTTACAAAAATTTATGGCTCGTGTGTCCAG
AGAAACAGGCCGTTTTTGTATCACCGCAACACAAATGCTCGAGTCAATGATTCGCAATCCCCTTCCTACACGAGCCGAAG
TTTCCGATGTAGCTAATGCTATCCACGATGGAACTTCCGCTGTGATGTTATCAGGAGAAACTGCTTCAGGAACTTATCCT
ATAGAAGCTGTAAAAACTATGCGCTCGATCATCCAAGAAACGGAAAAATCCTTTGATTACCAAGCCTTTTTCCAACTCAA
TGACAAAAATAGCGCTCTCAAAGTCTCTCCTTATCTTGAAGCAATAGGCGCTTCAGGGATCCAAATCGCTGAGAAAGCTT
CTGCTAAAGCGATTATTGTATACACCCAAACTGGGGGATCTCCCATGTTTCTTTCTAAATATCGTCCCTATCTCCCCATT
ATTGCCGTTACCCCAAACCGCAATGTATACTATCGCTTAGCAGTAGAATGGGGCGTATACCCTATGCTAACCTCAGAATC
TAACCGAACAGTTTGGCGCCACCAAGCTTGTGTCTATGGAGTAGAGAAAGGAATCCTTTCAAACTATGATAAAATTCTTG
TTTTTAGCCGAGGAGCAGGGATGCAGGACACGAATAACCTTACTCTGACTACTGTAAACGATGTTTTATCTCCTTCTCTT
GAATGA

Upstream 100 bases:

>100_bases
TACCCCTCCCCATCGCTAAATATTTTGTATAGTTGAAGGCTTCTTTCATTTTGGATATCCTAAGAAAATATTCCTCTTAC
TAATACTGGGATCCCGCTTT

Downstream 100 bases:

>100_bases
CCAACTCTGTAAATTTTTAACCTATATAGTTTACGAGCATGCCTTCTATTGTTAGACTTTCCGGTATCACCGTAAGAAAC
TTAAAAAATGTTACGGTAGA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MIARTKIICTIGPATNTPEMLEKLLDAGMNVARLNFSHGTHESHGRTIAILKELREKRQVPLAIMLDTKGPEIRLGQVES
PIKVKPGDRLTLTSKEILGSKEAGVTLYPSCVFPFVRERAPVLIDDGYIQAVVVNAQEHLIEIEFQNSGEIKSNKSLSIK
DIDVALPFMTEKDITDLKFGVEQELDLIAASFVRCNEDIDSMRKVLENFGRPNMPIIAKIENHLGVQNFQEIAKASDGIM
IARGDLGIELSIVEVPALQKFMARVSRETGRFCITATQMLESMIRNPLPTRAEVSDVANAIHDGTSAVMLSGETASGTYP
IEAVKTMRSIIQETEKSFDYQAFFQLNDKNSALKVSPYLEAIGASGIQIAEKASAKAIIVYTQTGGSPMFLSKYRPYLPI
IAVTPNRNVYYRLAVEWGVYPMLTSESNRTVWRHQACVYGVEKGILSNYDKILVFSRGAGMQDTNNLTLTTVNDVLSPSL
E

Sequences:

>Translated_481_residues
MIARTKIICTIGPATNTPEMLEKLLDAGMNVARLNFSHGTHESHGRTIAILKELREKRQVPLAIMLDTKGPEIRLGQVES
PIKVKPGDRLTLTSKEILGSKEAGVTLYPSCVFPFVRERAPVLIDDGYIQAVVVNAQEHLIEIEFQNSGEIKSNKSLSIK
DIDVALPFMTEKDITDLKFGVEQELDLIAASFVRCNEDIDSMRKVLENFGRPNMPIIAKIENHLGVQNFQEIAKASDGIM
IARGDLGIELSIVEVPALQKFMARVSRETGRFCITATQMLESMIRNPLPTRAEVSDVANAIHDGTSAVMLSGETASGTYP
IEAVKTMRSIIQETEKSFDYQAFFQLNDKNSALKVSPYLEAIGASGIQIAEKASAKAIIVYTQTGGSPMFLSKYRPYLPI
IAVTPNRNVYYRLAVEWGVYPMLTSESNRTVWRHQACVYGVEKGILSNYDKILVFSRGAGMQDTNNLTLTTVNDVLSPSL
E
>Mature_481_residues
MIARTKIICTIGPATNTPEMLEKLLDAGMNVARLNFSHGTHESHGRTIAILKELREKRQVPLAIMLDTKGPEIRLGQVES
PIKVKPGDRLTLTSKEILGSKEAGVTLYPSCVFPFVRERAPVLIDDGYIQAVVVNAQEHLIEIEFQNSGEIKSNKSLSIK
DIDVALPFMTEKDITDLKFGVEQELDLIAASFVRCNEDIDSMRKVLENFGRPNMPIIAKIENHLGVQNFQEIAKASDGIM
IARGDLGIELSIVEVPALQKFMARVSRETGRFCITATQMLESMIRNPLPTRAEVSDVANAIHDGTSAVMLSGETASGTYP
IEAVKTMRSIIQETEKSFDYQAFFQLNDKNSALKVSPYLEAIGASGIQIAEKASAKAIIVYTQTGGSPMFLSKYRPYLPI
IAVTPNRNVYYRLAVEWGVYPMLTSESNRTVWRHQACVYGVEKGILSNYDKILVFSRGAGMQDTNNLTLTTVNDVLSPSL
E

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=482, Percent_Identity=39.6265560165975, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI33286420, Length=482, Percent_Identity=39.6265560165975, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI33286418, Length=482, Percent_Identity=39.6265560165975, Blast_Score=318, Evalue=6e-87,
Organism=Homo sapiens, GI32967597, Length=466, Percent_Identity=40.343347639485, Blast_Score=312, Evalue=5e-85,
Organism=Homo sapiens, GI10835121, Length=466, Percent_Identity=40.343347639485, Blast_Score=311, Evalue=7e-85,
Organism=Homo sapiens, GI310128732, Length=286, Percent_Identity=41.958041958042, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI310128730, Length=286, Percent_Identity=41.958041958042, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=40.4255319148936, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=40.4255319148936, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=38.8888888888889, Blast_Score=139, Evalue=7e-33,
Organism=Escherichia coli, GI1787965, Length=435, Percent_Identity=43.2183908045977, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI1788160, Length=469, Percent_Identity=34.7547974413646, Blast_Score=232, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17544584, Length=495, Percent_Identity=39.1919191919192, Blast_Score=309, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17506829, Length=491, Percent_Identity=38.9002036659878, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71984413, Length=491, Percent_Identity=38.9002036659878, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71984406, Length=491, Percent_Identity=38.9002036659878, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17506831, Length=491, Percent_Identity=38.9002036659878, Blast_Score=275, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6319279, Length=488, Percent_Identity=38.9344262295082, Blast_Score=289, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6324923, Length=483, Percent_Identity=37.2670807453416, Blast_Score=275, Evalue=1e-74,
Organism=Drosophila melanogaster, GI28571814, Length=489, Percent_Identity=40.8997955010225, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=40.8997955010225, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI24648966, Length=415, Percent_Identity=38.7951807228916, Blast_Score=271, Evalue=7e-73,
Organism=Drosophila melanogaster, GI24581235, Length=483, Percent_Identity=31.2629399585921, Blast_Score=228, Evalue=6e-60,
Organism=Drosophila melanogaster, GI24646914, Length=265, Percent_Identity=37.3584905660377, Blast_Score=163, Evalue=3e-40,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53176; Mature: 53176

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIARTKIICTIGPATNTPEMLEKLLDAGMNVARLNFSHGTHESHGRTIAILKELREKRQV
CCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCC
PLAIMLDTKGPEIRLGQVESPIKVKPGDRLTLTSKEILGSKEAGVTLYPSCVFPFVRERA
CEEEEEECCCCCEEECCCCCCEEECCCCEEEEEHHHHCCCCCCCEEEEHHHHHHHHHCCC
PVLIDDGYIQAVVVNAQEHLIEIEFQNSGEIKSNKSLSIKDIDVALPFMTEKDITDLKFG
CEEEECCEEEEEEEECCCEEEEEEECCCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHCC
VEQELDLIAASFVRCNEDIDSMRKVLENFGRPNMPIIAKIENHLGVQNFQEIAKASDGIM
CHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEHHCCCHHHHHHHHHCCCCEE
IARGDLGIELSIVEVPALQKFMARVSRETGRFCITATQMLESMIRNPLPTRAEVSDVANA
EEECCCCEEEEEEECHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHH
IHDGTSAVMLSGETASGTYPIEAVKTMRSIIQETEKSFDYQAFFQLNDKNSALKVSPYLE
HCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECHHHH
AIGASGIQIAEKASAKAIIVYTQTGGSPMFLSKYRPYLPIIAVTPNRNVYYRLAVEWGVY
HHCCCCCEEHHCCCCCEEEEEEECCCCEEECCCCCCCCEEEEECCCCCEEEEEEEECCCE
PMLTSESNRTVWRHQACVYGVEKGILSNYDKILVFSRGAGMQDTNNLTLTTVNDVLSPSL
EEEECCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEHHHCCCCC
E
C
>Mature Secondary Structure
MIARTKIICTIGPATNTPEMLEKLLDAGMNVARLNFSHGTHESHGRTIAILKELREKRQV
CCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCC
PLAIMLDTKGPEIRLGQVESPIKVKPGDRLTLTSKEILGSKEAGVTLYPSCVFPFVRERA
CEEEEEECCCCCEEECCCCCCEEECCCCEEEEEHHHHCCCCCCCEEEEHHHHHHHHHCCC
PVLIDDGYIQAVVVNAQEHLIEIEFQNSGEIKSNKSLSIKDIDVALPFMTEKDITDLKFG
CEEEECCEEEEEEEECCCEEEEEEECCCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHCC
VEQELDLIAASFVRCNEDIDSMRKVLENFGRPNMPIIAKIENHLGVQNFQEIAKASDGIM
CHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEHHCCCHHHHHHHHHCCCCEE
IARGDLGIELSIVEVPALQKFMARVSRETGRFCITATQMLESMIRNPLPTRAEVSDVANA
EEECCCCEEEEEEECHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHH
IHDGTSAVMLSGETASGTYPIEAVKTMRSIIQETEKSFDYQAFFQLNDKNSALKVSPYLE
HCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECHHHH
AIGASGIQIAEKASAKAIIVYTQTGGSPMFLSKYRPYLPIIAVTPNRNVYYRLAVEWGVY
HHCCCCCEEHHCCCCCEEEEEEECCCCEEECCCCCCCCEEEEECCCCCEEEEEEEECCCE
PMLTSESNRTVWRHQACVYGVEKGILSNYDKILVFSRGAGMQDTNNLTLTTVNDVLSPSL
EEEECCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEHHHCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10411734 [H]