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Identifier: 15835180

GI number: 15835180

Start: 674863

End: 675534

Strand: Reverse

Name: Not Available

Synonym: TC0563

Alternate gene names: 15835180

Gene position: 675534-674863 (Counterclockwise)

Preceding gene: 15835181

Following gene: 15835179

Centisome position: 62.96

GC content: 39.14

Gene sequence:

>672_bases
ATGGATTTGCGATTAGATGAATTAGCTGTATTATTGGACGTTGCGGAAGATTCTATTCGGCAATGGGTGTCTTTCGAGGG
GATTCCTAGTTACAAAATCAATGATGAATTGCGCTTTAATAGAGAAGAAGTTGAGGACTGGCTCATCCATAATCACGGGG
TTATTGTGGCTGAGAAGGATAAGAGGGAATCTAAAGATTTGTCTCTTAGATACAGTCTTTACCGAGCTATTTACCGAGGA
GGGGTCCTTAAAAACGTCCTTGTAGATAGTAAAGAAGAAGCCTTACAGCATACAGCTAAGTATATAGCTAATAAGTTTGA
GTTGGATTCTGGAGTACTCTTCGAAATGCTTATGCATCGGGAGAGCCTTATGTCTACCGGTATTGGAGAGGGGATTGCTT
TGCCTCATGCCAAAGATTTTTTGATTAATATTGGATATGACATTGTTGTTCCTGTTTTCTTATCCCAGAGCATTGAATAC
GGGGCTTTGGATGGTAAGCCTGTTGATACTATGTTTTTTCTCTTTGCAAACCAAGATAGACATCATCTGAATTTAGTGAA
TAAAATTGTGCATTTGGGAATGTCTTTAGAAGCTCGTCTTTTTCTTAAGGAACACCCGGATCAGCCGCAGCTTTTAGCTT
TTATTAAGAATTGGGAAGCTCGAATTCATTAA

Upstream 100 bases:

>100_bases
TCAGCATTAACACAATCTTTACGAGATGAAGCTAGAAGGTCACAACTTCTACAGGTGCAAACTGTTGAAGAAGTGATGAG
CATTTTTCAGGAGTCTAGTT

Downstream 100 bases:

>100_bases
GTTACCAATAAAAAAGAATTTTACGTCCAAGTTGTGAAAAAAGCTTTAATGTTGCTATGGTGATCCCTAAGGTGGATCTA
GGAGAAAGTGCCGTCATGAT

Product: PTS system, IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MDLRLDELAVLLDVAEDSIRQWVSFEGIPSYKINDELRFNREEVEDWLIHNHGVIVAEKDKRESKDLSLRYSLYRAIYRG
GVLKNVLVDSKEEALQHTAKYIANKFELDSGVLFEMLMHRESLMSTGIGEGIALPHAKDFLINIGYDIVVPVFLSQSIEY
GALDGKPVDTMFFLFANQDRHHLNLVNKIVHLGMSLEARLFLKEHPDQPQLLAFIKNWEARIH

Sequences:

>Translated_223_residues
MDLRLDELAVLLDVAEDSIRQWVSFEGIPSYKINDELRFNREEVEDWLIHNHGVIVAEKDKRESKDLSLRYSLYRAIYRG
GVLKNVLVDSKEEALQHTAKYIANKFELDSGVLFEMLMHRESLMSTGIGEGIALPHAKDFLINIGYDIVVPVFLSQSIEY
GALDGKPVDTMFFLFANQDRHHLNLVNKIVHLGMSLEARLFLKEHPDQPQLLAFIKNWEARIH
>Mature_223_residues
MDLRLDELAVLLDVAEDSIRQWVSFEGIPSYKINDELRFNREEVEDWLIHNHGVIVAEKDKRESKDLSLRYSLYRAIYRG
GVLKNVLVDSKEEALQHTAKYIANKFELDSGVLFEMLMHRESLMSTGIGEGIALPHAKDFLINIGYDIVVPVFLSQSIEY
GALDGKPVDTMFFLFANQDRHHLNLVNKIVHLGMSLEARLFLKEHPDQPQLLAFIKNWEARIH

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=124, Percent_Identity=36.2903225806452, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 25731; Mature: 25731

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRLDELAVLLDVAEDSIRQWVSFEGIPSYKINDELRFNREEVEDWLIHNHGVIVAEKD
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCEEEEECC
KRESKDLSLRYSLYRAIYRGGVLKNVLVDSKEEALQHTAKYIANKFELDSGVLFEMLMHR
CCCCCCHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ESLMSTGIGEGIALPHAKDFLINIGYDIVVPVFLSQSIEYGALDGKPVDTMFFLFANQDR
HHHHHCCCCCCCCCCCHHHHEEECCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCC
HHLNLVNKIVHLGMSLEARLFLKEHPDQPQLLAFIKNWEARIH
HHHHHHHHHHHHCCCHHEEEHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLRLDELAVLLDVAEDSIRQWVSFEGIPSYKINDELRFNREEVEDWLIHNHGVIVAEKD
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCEEEEECC
KRESKDLSLRYSLYRAIYRGGVLKNVLVDSKEEALQHTAKYIANKFELDSGVLFEMLMHR
CCCCCCHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ESLMSTGIGEGIALPHAKDFLINIGYDIVVPVFLSQSIEYGALDGKPVDTMFFLFANQDR
HHHHHCCCCCCCCCCCHHHHEEECCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCC
HHLNLVNKIVHLGMSLEARLFLKEHPDQPQLLAFIKNWEARIH
HHHHHHHHHHHHCCCHHEEEHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]