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The map label for this gene is mraW

Identifier: 15835162

GI number: 15835162

Start: 654164

End: 655072

Strand: Reverse

Name: mraW

Synonym: TC0544

Alternate gene names: 15835162

Gene position: 655072-654164 (Counterclockwise)

Preceding gene: 15835167

Following gene: 15835161

Centisome position: 61.05

GC content: 42.02

Gene sequence:

>909_bases
GTGACTGATTCCATTCCTCATATTCCCGTTTTGGTAAAAGAGAGTCTTTCATTATTTCAGGACCAGCATCCTACTACTTT
TTGTGACGTTACTGTCGGCGCAGGTGGTCATGCAGAAGCTTTTCTAACTTCATTCCCCTCAATAGAACATTATGATGGGT
CCGACAGAGATCTGTCTGCTCTGGCTTTGTCAGAGAAAAGGTTGCTTCCTTTCAAGGGGCGAGTGTGTTTGCGTCATGCC
TCGTTTGATGAGGTTAACGCGCTAACTCCTGATGGGATGTATGATGGGGTGCTTGCTGATTTAGGAGTTTCCTCTATGCA
ATTAACGGACCTTGAACGAGGATTTAGTTTTCAAGGGGAGGATCACCCCTTAGATATGCGAATGGATACTTCTCGAGGTA
TTACTGCTAGTGAGGTGCTGAATTCTTTAAGAGAAGAGGAAATAGGAGAAATTTTCCGAAAATATGGAGAAGAGCCTCTT
TGGAGGAGCGCTGCAAATGCTGTGGTTCATTTTAGGAAAAAGAAAAAGATTTTAACGGTTAAGGATCTAAAGGATGCAAC
GTCAGGGGTTTTCCCATCCTATCGATTAAGAAAAAAAATTCATCCATTAACATTGATATTTCAAGCTTTGCGCATTTATG
TTAATCAAGAAGGGGCGCAATTAAAAGTTTTATTGGATTCCGCTTTGCGTTGGCTGCGTCCTGGTGGTCGTTTAGCTATC
ATTTCTTTTTGTAGTTTAGATGATCGTCCTGTTAAGTGGGCTTTTAGAGAAGCTGAGGCAAGAGGAGAGGGGCGAGTATT
GACCAAAAAGGTAATCATGCCAACTTATGAAGAAACTAGGAAGAATCCTCGATCACGCTCGGCTAAGTTGCGGTGTTTTG
AGAAGAGTTTAAAGATAAGTGATGAATAG

Upstream 100 bases:

>100_bases
ATACGATAAACTAAAGCCTTTTACTTTAGCTGCAAGAGAACGTTTTGAAGAGAGTGTTTTTGCGGCTTAATAGGATAATT
TTTTATGTAAAAGGTTTCTT

Downstream 100 bases:

>100_bases
GTATCGGTTCTCGCGGCTGTTTGTATGTTTAAGTCTTTTGTGCTGCGCTCTTTATCGGTATATAGATAAACAAAATGATC
TTACTAAACTTCGGTTAGAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTDSIPHIPVLVKESLSLFQDQHPTTFCDVTVGAGGHAEAFLTSFPSIEHYDGSDRDLSALALSEKRLLPFKGRVCLRHA
SFDEVNALTPDGMYDGVLADLGVSSMQLTDLERGFSFQGEDHPLDMRMDTSRGITASEVLNSLREEEIGEIFRKYGEEPL
WRSAANAVVHFRKKKKILTVKDLKDATSGVFPSYRLRKKIHPLTLIFQALRIYVNQEGAQLKVLLDSALRWLRPGGRLAI
ISFCSLDDRPVKWAFREAEARGEGRVLTKKVIMPTYEETRKNPRSRSAKLRCFEKSLKISDE

Sequences:

>Translated_302_residues
MTDSIPHIPVLVKESLSLFQDQHPTTFCDVTVGAGGHAEAFLTSFPSIEHYDGSDRDLSALALSEKRLLPFKGRVCLRHA
SFDEVNALTPDGMYDGVLADLGVSSMQLTDLERGFSFQGEDHPLDMRMDTSRGITASEVLNSLREEEIGEIFRKYGEEPL
WRSAANAVVHFRKKKKILTVKDLKDATSGVFPSYRLRKKIHPLTLIFQALRIYVNQEGAQLKVLLDSALRWLRPGGRLAI
ISFCSLDDRPVKWAFREAEARGEGRVLTKKVIMPTYEETRKNPRSRSAKLRCFEKSLKISDE
>Mature_301_residues
TDSIPHIPVLVKESLSLFQDQHPTTFCDVTVGAGGHAEAFLTSFPSIEHYDGSDRDLSALALSEKRLLPFKGRVCLRHAS
FDEVNALTPDGMYDGVLADLGVSSMQLTDLERGFSFQGEDHPLDMRMDTSRGITASEVLNSLREEEIGEIFRKYGEEPLW
RSAANAVVHFRKKKKILTVKDLKDATSGVFPSYRLRKKIHPLTLIFQALRIYVNQEGAQLKVLLDSALRWLRPGGRLAII
SFCSLDDRPVKWAFREAEARGEGRVLTKKVIMPTYEETRKNPRSRSAKLRCFEKSLKISDE

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=338, Percent_Identity=36.9822485207101, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI165377202, Length=198, Percent_Identity=38.8888888888889, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1786270, Length=321, Percent_Identity=35.8255451713396, Blast_Score=165, Evalue=3e-42,
Organism=Drosophila melanogaster, GI62472493, Length=334, Percent_Identity=33.8323353293413, Blast_Score=140, Evalue=9e-34,
Organism=Drosophila melanogaster, GI28571637, Length=334, Percent_Identity=33.8323353293413, Blast_Score=140, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_CHLMU (Q9PKC2)

Other databases:

- EMBL:   AE002160
- PIR:   D81692
- RefSeq:   NP_296921.1
- ProteinModelPortal:   Q9PKC2
- SMR:   Q9PKC2
- GeneID:   1245904
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0544
- TIGR:   TC_0544
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   CMUR243161:TC_0544-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34098; Mature: 33966

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 82-82 BINDING 100-100 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSIPHIPVLVKESLSLFQDQHPTTFCDVTVGAGGHAEAFLTSFPSIEHYDGSDRDLSA
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCHHH
LALSEKRLLPFKGRVCLRHASFDEVNALTPDGMYDGVLADLGVSSMQLTDLERGFSFQGE
HHHCCCCCCCCCCCEEEEECCCHHHCCCCCCCCHHHHHHHCCCCCEEHHHHHCCCCCCCC
DHPLDMRMDTSRGITASEVLNSLREEEIGEIFRKYGEEPLWRSAANAVVHFRKKKKILTV
CCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEH
KDLKDATSGVFPSYRLRKKIHPLTLIFQALRIYVNQEGAQLKVLLDSALRWLRPGGRLAI
HHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCEEE
ISFCSLDDRPVKWAFREAEARGEGRVLTKKVIMPTYEETRKNPRSRSAKLRCFEKSLKIS
EEEECCCCCCHHHHHHHHHCCCCCCEEEHHHCCCCHHHHHCCCCCCHHHHHHHHHCCCCC
DE
CC
>Mature Secondary Structure 
TDSIPHIPVLVKESLSLFQDQHPTTFCDVTVGAGGHAEAFLTSFPSIEHYDGSDRDLSA
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCHHH
LALSEKRLLPFKGRVCLRHASFDEVNALTPDGMYDGVLADLGVSSMQLTDLERGFSFQGE
HHHCCCCCCCCCCCEEEEECCCHHHCCCCCCCCHHHHHHHCCCCCEEHHHHHCCCCCCCC
DHPLDMRMDTSRGITASEVLNSLREEEIGEIFRKYGEEPLWRSAANAVVHFRKKKKILTV
CCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEH
KDLKDATSGVFPSYRLRKKIHPLTLIFQALRIYVNQEGAQLKVLLDSALRWLRPGGRLAI
HHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCEEE
ISFCSLDDRPVKWAFREAEARGEGRVLTKKVIMPTYEETRKNPRSRSAKLRCFEKSLKIS
EEEECCCCCCHHHHHHHHHCCCCCCEEEHHHCCCCHHHHHCCCCCCHHHHHHHHHCCCCC
DE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935