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Identifier: 15835064

GI number: 15835064

Start: 543868

End: 545451

Strand: Direct

Name: Not Available

Synonym: TC0446

Alternate gene names: 15835064

Gene position: 543868-545451 (Clockwise)

Preceding gene: 15835062

Following gene: 15835065

Centisome position: 50.69

GC content: 35.42

Gene sequence:

>1584_bases
ATGCCTAAAAAAAAGTTTTTATATATCTTGATCCCCTTCTTGAGCCTTACTTTTGGCTTAACGTCTTGTCACCAGAAAGA
AGAAAACCTAAGAAATATTTTGCGTGTTGCTATTTGCCACGATCCAATGTCTTTAGATCCTCGCCAAGTTTTTTTGATTA
AAGATGTTTGTATAGCAAAAGCACTTTATGAAGGATTGGTTCGAGAAAATGATGGCAGTCTTCATTTGGCTCTAGCAGAA
AGATACAGCTTGTCCCAGGATCGGTGTGTCTACACTTTTTTTCTAAAAAAAACTTTTTGGCATAACGGAGATCTTGTAAC
AGCATATGATTTTGAAGAGTCTATAAAGCAATTTTATCTCCATGAGGTTGATAATGTTGCGTTACGATTGCTTGCGTTAA
TTAAAAATTCTCATGCTGTTCTAAAAGGAGACATTCCTGTTGAAAATTTAGGTGTTCGCGCTTTAGATGAACATACTCTT
GAAATCACTCTAGAACACCCCTCTTCTCATTTTTTAGAAACTCTCACACATCCCGTTTTTTATCCAGTGCATGCTTCTTT
GAGAGAATATTATAGAAATCGAAGTAAACGCTCTCTTCCAATAATTTCTAACGGGCCTTTTATCATTCGGTGTTATGAGC
CACAAAATTTTTTGTTGCTTGATAAAAATCCTTTTTATCATGACCAAAAAAATGTTTCTTTAGATGCTGTACGCTTACAA
ATAGTTCCCGATATTCATACAGCGGTGCAATTGTTTCAAAAAAAATATGTGGATTTAGTGGGGTTGCCTTGGAGCTCCTC
CTTCCCTCTAGAAGAACAAAAAAATCTTTCTCAAGATTTTTTGTATGATTATCCGGTGCTAAATTGCACAGTTCTATTCT
GCAATGTTAACCACAAACCATTAGATAATCCTTCGCTAAGAGCGGCACTCTCTTTAGCCATTGATCGAGAAACATTATTA
AAATTAGCTGGCAAAGGAAGCATTGCTACCAGTTTTGTACATCCTAGTCTATCTAAGATGCCTTTAGATGTTCTTTCGCA
AAAAGAGAGAATCTCTTTAGCTAAAAATTACCTAGCGGAAGCTTTAAAAACTGTTCCTCAAGAAGAGTTAAAAAAAATCA
CACTCATCTACCCCATAGAATCTATTGTCTTGCGAGCAGTTGTTCAAGAGATTCGTCAACAGTTATTTGATGTTTTAGGA
TTTAAGATTTCTACTTTAGGATTAGAATACCACAGTTTTTTGGATAAACGTTCTAAAGGAGAGTTTTCTTTATCAACAGG
AAATTGGGTTGCTGATTATCAACAAGCCAAAGCTTTTCTATCCATCCTTGGTAATGGGACTAAATATAAAGATTATCAAG
TGATTGATTGGCAAAATCAAGAATATACGGATATCGTATCTCGACTTTTGGTTGAGGATTCAACAGATTTACAAATTCTA
GCAGAACAACTATTGCTCAAAGAATCCCCTCTTATTCCCCTTTATCACTTGGATTATGCTTACGCTAAACATCCTAAAGT
CTCTAATCTCCAAACCTCCTCACTCGGAGAAATTGATTTAAAAAGAGTCTCGTTAGTGGAGTAA

Upstream 100 bases:

>100_bases
CAGCGTCTTTATATCTATGGTTTAGAGTTTGATAGGTCAAGCAGTTCAAAATTAGAAGAAGTTTAGTGCTAATTTGATTT
TGATTAGTTGCCGAAACTTT

Downstream 100 bases:

>100_bases
GTGTGGAAAATGCTCGCAACAAGCAAGATAGGCTCTTAAACCTCCAAGTACGCGACTCCCGTAGTTTCGATATTCAGCAC
TAACGATGCTCTTACTGCTA

Product: peptide ABC transporter, periplasmic peptide-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MPKKKFLYILIPFLSLTFGLTSCHQKEENLRNILRVAICHDPMSLDPRQVFLIKDVCIAKALYEGLVRENDGSLHLALAE
RYSLSQDRCVYTFFLKKTFWHNGDLVTAYDFEESIKQFYLHEVDNVALRLLALIKNSHAVLKGDIPVENLGVRALDEHTL
EITLEHPSSHFLETLTHPVFYPVHASLREYYRNRSKRSLPIISNGPFIIRCYEPQNFLLLDKNPFYHDQKNVSLDAVRLQ
IVPDIHTAVQLFQKKYVDLVGLPWSSSFPLEEQKNLSQDFLYDYPVLNCTVLFCNVNHKPLDNPSLRAALSLAIDRETLL
KLAGKGSIATSFVHPSLSKMPLDVLSQKERISLAKNYLAEALKTVPQEELKKITLIYPIESIVLRAVVQEIRQQLFDVLG
FKISTLGLEYHSFLDKRSKGEFSLSTGNWVADYQQAKAFLSILGNGTKYKDYQVIDWQNQEYTDIVSRLLVEDSTDLQIL
AEQLLLKESPLIPLYHLDYAYAKHPKVSNLQTSSLGEIDLKRVSLVE

Sequences:

>Translated_527_residues
MPKKKFLYILIPFLSLTFGLTSCHQKEENLRNILRVAICHDPMSLDPRQVFLIKDVCIAKALYEGLVRENDGSLHLALAE
RYSLSQDRCVYTFFLKKTFWHNGDLVTAYDFEESIKQFYLHEVDNVALRLLALIKNSHAVLKGDIPVENLGVRALDEHTL
EITLEHPSSHFLETLTHPVFYPVHASLREYYRNRSKRSLPIISNGPFIIRCYEPQNFLLLDKNPFYHDQKNVSLDAVRLQ
IVPDIHTAVQLFQKKYVDLVGLPWSSSFPLEEQKNLSQDFLYDYPVLNCTVLFCNVNHKPLDNPSLRAALSLAIDRETLL
KLAGKGSIATSFVHPSLSKMPLDVLSQKERISLAKNYLAEALKTVPQEELKKITLIYPIESIVLRAVVQEIRQQLFDVLG
FKISTLGLEYHSFLDKRSKGEFSLSTGNWVADYQQAKAFLSILGNGTKYKDYQVIDWQNQEYTDIVSRLLVEDSTDLQIL
AEQLLLKESPLIPLYHLDYAYAKHPKVSNLQTSSLGEIDLKRVSLVE
>Mature_526_residues
PKKKFLYILIPFLSLTFGLTSCHQKEENLRNILRVAICHDPMSLDPRQVFLIKDVCIAKALYEGLVRENDGSLHLALAER
YSLSQDRCVYTFFLKKTFWHNGDLVTAYDFEESIKQFYLHEVDNVALRLLALIKNSHAVLKGDIPVENLGVRALDEHTLE
ITLEHPSSHFLETLTHPVFYPVHASLREYYRNRSKRSLPIISNGPFIIRCYEPQNFLLLDKNPFYHDQKNVSLDAVRLQI
VPDIHTAVQLFQKKYVDLVGLPWSSSFPLEEQKNLSQDFLYDYPVLNCTVLFCNVNHKPLDNPSLRAALSLAIDRETLLK
LAGKGSIATSFVHPSLSKMPLDVLSQKERISLAKNYLAEALKTVPQEELKKITLIYPIESIVLRAVVQEIRQQLFDVLGF
KISTLGLEYHSFLDKRSKGEFSLSTGNWVADYQQAKAFLSILGNGTKYKDYQVIDWQNQEYTDIVSRLLVEDSTDLQILA
EQLLLKESPLIPLYHLDYAYAKHPKVSNLQTSSLGEIDLKRVSLVE

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=477, Percent_Identity=27.4633123689727, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1789397, Length=480, Percent_Identity=26.875, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI87081878, Length=515, Percent_Identity=25.631067961165, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787052, Length=486, Percent_Identity=24.8971193415638, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787762, Length=426, Percent_Identity=22.5352112676056, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789966, Length=409, Percent_Identity=23.7163814180929, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789887, Length=498, Percent_Identity=23.2931726907631, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60453; Mature: 60322

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKKKFLYILIPFLSLTFGLTSCHQKEENLRNILRVAICHDPMSLDPRQVFLIKDVCIAK
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHH
ALYEGLVRENDGSLHLALAERYSLSQDRCVYTFFLKKTFWHNGDLVTAYDFEESIKQFYL
HHHHHHHCCCCCCEEEEEEHHCCCCCCCEEEEEEEHHHHCCCCCEEEEECHHHHHHHHHH
HEVDNVALRLLALIKNSHAVLKGDIPVENLGVRALDEHTLEITLEHPSSHFLETLTHPVF
HHHHHHHHHHHHHHHCCCEEEECCCCHHHCCCEEECCCEEEEEEECCCHHHHHHHCCCEE
YPVHASLREYYRNRSKRSLPIISNGPFIIRCYEPQNFLLLDKNPFYHDQKNVSLDAVRLQ
EHHHHHHHHHHHCCCCCCCCEEECCCEEEEEECCCCEEEECCCCCCCCCCCCEEEEEEEE
IVPDIHTAVQLFQKKYVDLVGLPWSSSFPLEEQKNLSQDFLYDYPVLNCTVLFCNVNHKP
EECCHHHHHHHHHHHHHHEEECCCCCCCCCHHHHCCCHHHHHCCCCEEEEEEEEECCCCC
LDNPSLRAALSLAIDRETLLKLAGKGSIATSFVHPSLSKMPLDVLSQKERISLAKNYLAE
CCCCHHHHHHHHHHCHHHHHHHCCCCCEEEHHCCCCHHCCCHHHHCCHHHHHHHHHHHHH
ALKTVPQEELKKITLIYPIESIVLRAVVQEIRQQLFDVLGFKISTLGLEYHSFLDKRSKG
HHHHCCHHHHCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHCCCCC
EFSLSTGNWVADYQQAKAFLSILGNGTKYKDYQVIDWQNQEYTDIVSRLLVEDSTDLQIL
CEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHH
AEQLLLKESPLIPLYHLDYAYAKHPKVSNLQTSSLGEIDLKRVSLVE
HHHHHHHCCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
PKKKFLYILIPFLSLTFGLTSCHQKEENLRNILRVAICHDPMSLDPRQVFLIKDVCIAK
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHH
ALYEGLVRENDGSLHLALAERYSLSQDRCVYTFFLKKTFWHNGDLVTAYDFEESIKQFYL
HHHHHHHCCCCCCEEEEEEHHCCCCCCCEEEEEEEHHHHCCCCCEEEEECHHHHHHHHHH
HEVDNVALRLLALIKNSHAVLKGDIPVENLGVRALDEHTLEITLEHPSSHFLETLTHPVF
HHHHHHHHHHHHHHHCCCEEEECCCCHHHCCCEEECCCEEEEEEECCCHHHHHHHCCCEE
YPVHASLREYYRNRSKRSLPIISNGPFIIRCYEPQNFLLLDKNPFYHDQKNVSLDAVRLQ
EHHHHHHHHHHHCCCCCCCCEEECCCEEEEEECCCCEEEECCCCCCCCCCCCEEEEEEEE
IVPDIHTAVQLFQKKYVDLVGLPWSSSFPLEEQKNLSQDFLYDYPVLNCTVLFCNVNHKP
EECCHHHHHHHHHHHHHHEEECCCCCCCCCHHHHCCCHHHHHCCCCEEEEEEEEECCCCC
LDNPSLRAALSLAIDRETLLKLAGKGSIATSFVHPSLSKMPLDVLSQKERISLAKNYLAE
CCCCHHHHHHHHHHCHHHHHHHCCCCCEEEHHCCCCHHCCCHHHHCCHHHHHHHHHHHHH
ALKTVPQEELKKITLIYPIESIVLRAVVQEIRQQLFDVLGFKISTLGLEYHSFLDKRSKG
HHHHCCHHHHCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHCCCCC
EFSLSTGNWVADYQQAKAFLSILGNGTKYKDYQVIDWQNQEYTDIVSRLLVEDSTDLQIL
CEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHH
AEQLLLKESPLIPLYHLDYAYAKHPKVSNLQTSSLGEIDLKRVSLVE
HHHHHHHCCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]