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The map label for this gene is phzF

Identifier: 16120293

GI number: 16120293

Start: 317427

End: 318326

Strand: Direct

Name: phzF

Synonym: VNG6408G

Alternate gene names: 16120293

Gene position: 317427-318326 (Clockwise)

Preceding gene: 16120285

Following gene: 16120295

Centisome position: 86.87

GC content: 52.22

Gene sequence:

>900_bases
ATGAGTGACATCCGCTATGATATCGTTGACGTCTTCGCACAAAGCCAATATACGGGGAATCAACTCGCGGTTGTCCAACC
AGAAGGTGAATTGAGCGGCGAAGAAATGCAGCGGATCGCGAACGAATTCGACTACTCAGAGACAACATTCATCGACCCAG
ATGGAAGTCATGCGGACGGATTCGAGGTCCGCATCTTCTCGCCCACGACAGAACTACCGTTCGCCGGCCACCCCACGCTC
GGTACGGCGTACGTTCTCCGAGAGCGGCTCGGACTTTCCGATGATGAACTAGTGTTACACGAACAAGTCGGCGCAATCCC
CGTTTCAGTCGAAAAAACAGCTACTGGTGATGAAATCTTACTAATGGAGCACCCGGAACCAACGTTCGATGAAATAATTG
AGCCTGCGAGGGCGGCGGACGCGGTTTCCCTACCAGCTGACGTTTTGGATGACCAGTTCCCAGCCCAGATTATCTCAACT
GGCGTTCCGACACTAATTGTGCCAGTTGAATCGCTAAGTGCGGTGAAGGAGGCCGAGACTAATGATGAAGTGTATTCGGA
ATTGGTTGATCAGTACGGAGTACGGAATCTCCTCGTGTTCGCTCCGGAGACATACGAACCAGAAAACGAGCTCAACGTCC
GAGTGTTTGCAGAGTTTTTCGGTATCCCTGAGGATCCTGCGACTGGTGCTTCGAATGGGTGTCTCGCTGCATACCTTACT
GAGCACCAGTACTTCGACACGTCTGAGATCGACGTACGTGTCGAACAAGGATATGAAGTGAACCGACCCTCACTACTGTA
TCTACGTGGGCAGACAACTGCAGATTCGATTCAGGTAGAGGTCGGTGGGCGAGTAATTCCGACTGCGACTGGGACTCTCG
TCGAGTCGAATAGCCTCTAA

Upstream 100 bases:

>100_bases
TAGGAGAGTAGAAAGCGCACTCCGAGTAAGAGAGACCAGTGACACTCGCCCTGACGAGACTACTTATCACCCAATCTCTG
TAAGTAAATACGTGAGCAGT

Downstream 100 bases:

>100_bases
ACTGGCTCCGATATCATTCTATTAGTTCGTTCGGGTTCTGCGGCAGAATTGATATCGTATGTCTATTTCCGCTGTGTCCT
CAGCTAAAGATCAATCAGTA

Product: phenazine biosynthetic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSDIRYDIVDVFAQSQYTGNQLAVVQPEGELSGEEMQRIANEFDYSETTFIDPDGSHADGFEVRIFSPTTELPFAGHPTL
GTAYVLRERLGLSDDELVLHEQVGAIPVSVEKTATGDEILLMEHPEPTFDEIIEPARAADAVSLPADVLDDQFPAQIIST
GVPTLIVPVESLSAVKEAETNDEVYSELVDQYGVRNLLVFAPETYEPENELNVRVFAEFFGIPEDPATGASNGCLAAYLT
EHQYFDTSEIDVRVEQGYEVNRPSLLYLRGQTTADSIQVEVGGRVIPTATGTLVESNSL

Sequences:

>Translated_299_residues
MSDIRYDIVDVFAQSQYTGNQLAVVQPEGELSGEEMQRIANEFDYSETTFIDPDGSHADGFEVRIFSPTTELPFAGHPTL
GTAYVLRERLGLSDDELVLHEQVGAIPVSVEKTATGDEILLMEHPEPTFDEIIEPARAADAVSLPADVLDDQFPAQIIST
GVPTLIVPVESLSAVKEAETNDEVYSELVDQYGVRNLLVFAPETYEPENELNVRVFAEFFGIPEDPATGASNGCLAAYLT
EHQYFDTSEIDVRVEQGYEVNRPSLLYLRGQTTADSIQVEVGGRVIPTATGTLVESNSL
>Mature_298_residues
SDIRYDIVDVFAQSQYTGNQLAVVQPEGELSGEEMQRIANEFDYSETTFIDPDGSHADGFEVRIFSPTTELPFAGHPTLG
TAYVLRERLGLSDDELVLHEQVGAIPVSVEKTATGDEILLMEHPEPTFDEIIEPARAADAVSLPADVLDDQFPAQIISTG
VPTLIVPVESLSAVKEAETNDEVYSELVDQYGVRNLLVFAPETYEPENELNVRVFAEFFGIPEDPATGASNGCLAAYLTE
HQYFDTSEIDVRVEQGYEVNRPSLLYLRGQTTADSIQVEVGGRVIPTATGTLVESNSL

Specific function: Isomerase that catalyzes the condensation of two molecules of trans-2,3-dihydro-3-hydroxyanthranilic acid (DHHA) into the phenazine ring system. The final product is not yet known [H]

COG id: COG0384

COG function: function code R; Predicted epimerase, PhzC/PhzF homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phzF family [H]

Homologues:

Organism=Homo sapiens, GI62177133, Length=244, Percent_Identity=25.8196721311475, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI74316009, Length=247, Percent_Identity=26.7206477732794, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1787737, Length=265, Percent_Identity=30.188679245283, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17542160, Length=309, Percent_Identity=27.1844660194175, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17542154, Length=309, Percent_Identity=27.1844660194175, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321818, Length=279, Percent_Identity=26.5232974910394, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003719 [H]

Pfam domain/function: PF02567 PhzC-PhzF [H]

EC number: NA

Molecular weight: Translated: 32737; Mature: 32606

Theoretical pI: Translated: 3.76; Mature: 3.76

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIRYDIVDVFAQSQYTGNQLAVVQPEGELSGEEMQRIANEFDYSETTFIDPDGSHADG
CCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCC
FEVRIFSPTTELPFAGHPTLGTAYVLRERLGLSDDELVLHEQVGAIPVSVEKTATGDEIL
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEHHCCCEEEEEECCCCCCEEE
LMEHPEPTFDEIIEPARAADAVSLPADVLDDQFPAQIISTGVPTLIVPVESLSAVKEAET
EEECCCCCHHHHHCHHHHCCCCCCCHHHHCCCCCHHHHHCCCCEEEEEHHHHHHHHHCCC
NDEVYSELVDQYGVRNLLVFAPETYEPENELNVRVFAEFFGIPEDPATGASNGCLAAYLT
CHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEEHHCCCCCCCCCCCCCEEEEEEE
EHQYFDTSEIDVRVEQGYEVNRPSLLYLRGQTTADSIQVEVGGRVIPTATGTLVESNSL
CCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCEEEEEECCEEECCCCCEEEECCCC
>Mature Secondary Structure 
SDIRYDIVDVFAQSQYTGNQLAVVQPEGELSGEEMQRIANEFDYSETTFIDPDGSHADG
CCCHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCC
FEVRIFSPTTELPFAGHPTLGTAYVLRERLGLSDDELVLHEQVGAIPVSVEKTATGDEIL
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEHHCCCEEEEEECCCCCCEEE
LMEHPEPTFDEIIEPARAADAVSLPADVLDDQFPAQIISTGVPTLIVPVESLSAVKEAET
EEECCCCCHHHHHCHHHHCCCCCCCHHHHCCCCCHHHHHCCCCEEEEEHHHHHHHHHCCC
NDEVYSELVDQYGVRNLLVFAPETYEPENELNVRVFAEFFGIPEDPATGASNGCLAAYLT
CHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEEHHCCCCCCCCCCCCCEEEEEEE
EHQYFDTSEIDVRVEQGYEVNRPSLLYLRGQTTADSIQVEVGGRVIPTATGTLVESNSL
CCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCEEEEEECCEEECCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]