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The map label for this gene is def

Identifier: 15836598

GI number: 15836598

Start: 1218069

End: 1218629

Strand: Direct

Name: def

Synonym: CPj1067

Alternate gene names: 15836598

Gene position: 1218069-1218629 (Clockwise)

Preceding gene: 15836597

Following gene: 15836600

Centisome position: 99.31

GC content: 40.11

Gene sequence:

>561_bases
ATGATTAGACGTTTAGAATATTACGGCAGTCCTATTTTAAGGAAAAAGTCTTCCCCAATTGCAGAGATCACAGATGAGAT
TCGTAATCTCGTGAGTGATATGTGTGATACTATGGAAGCACATCGTGGTGTCGGTTTAGCCGCTCCTCAGGTAGGGAAAA
ACGTCAGTTTATTTGTCATGTGTGTAGATAGAGAGACTGAGGATGGAGAGTTGATTTTCTCTGAGTCTCCGAGGGTATTT
ATCAATCCTGTTCTATCAGATCCTTCTGAAACCCCGATCATAGGTAAAGAAGGATGTCTTTCTATTCCTGGATTGCGAGG
AGAAGTATTCCGCCCTCAGAAAATCACAGTGACCGCTATGGATCTCAATGGTAAAATATTTACTGAGCACTTGGAAGGAT
TCACTGCACGTATCATTATGCACGAGACTGACCATCTGAATGGAGTTCTCTATATTGATCTTATGGAAGAACCCAAAGAT
CCTAAAAAATTTAAAGCCTCTTTAGAGAAGATCAAACGTCGCTACAATACACACTTGAGTAAAGAAGAACTAGTTTCTTA
A

Upstream 100 bases:

>100_bases
TTAAAGAGTATGACTCTTATCAACCCAAGAAATGTTTTAGATCCAAGTGCTTGTCGTACGAGATTTCTTCACAGAGCTCT
GCCAAGCCCATGTTCAGACT

Downstream 100 bases:

>100_bases
TTGCTCTTCAGTCTGATGTAGGTGATATTTTCTTGTCTCTTGCGTCACATTTGTTGTCAGCTTTGCTTATTTCCCCGAAC
AAATTTCGTCAAAGGTTTTA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MIRRLEYYGSPILRKKSSPIAEITDEIRNLVSDMCDTMEAHRGVGLAAPQVGKNVSLFVMCVDRETEDGELIFSESPRVF
INPVLSDPSETPIIGKEGCLSIPGLRGEVFRPQKITVTAMDLNGKIFTEHLEGFTARIIMHETDHLNGVLYIDLMEEPKD
PKKFKASLEKIKRRYNTHLSKEELVS

Sequences:

>Translated_186_residues
MIRRLEYYGSPILRKKSSPIAEITDEIRNLVSDMCDTMEAHRGVGLAAPQVGKNVSLFVMCVDRETEDGELIFSESPRVF
INPVLSDPSETPIIGKEGCLSIPGLRGEVFRPQKITVTAMDLNGKIFTEHLEGFTARIIMHETDHLNGVLYIDLMEEPKD
PKKFKASLEKIKRRYNTHLSKEELVS
>Mature_186_residues
MIRRLEYYGSPILRKKSSPIAEITDEIRNLVSDMCDTMEAHRGVGLAAPQVGKNVSLFVMCVDRETEDGELIFSESPRVF
INPVLSDPSETPIIGKEGCLSIPGLRGEVFRPQKITVTAMDLNGKIFTEHLEGFTARIIMHETDHLNGVLYIDLMEEPKD
PKKFKASLEKIKRRYNTHLSKEELVS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=165, Percent_Identity=32.1212121212121, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1789682, Length=162, Percent_Identity=40.1234567901235, Blast_Score=98, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24645726, Length=161, Percent_Identity=34.1614906832298, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24645728, Length=166, Percent_Identity=34.3373493975904, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_CHLPN (Q9Z6J2)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   E72000
- PIR:   H86623
- RefSeq:   NP_225261.1
- RefSeq:   NP_301122.1
- RefSeq:   NP_445322.1
- RefSeq:   NP_877382.1
- ProteinModelPortal:   Q9Z6J2
- SMR:   Q9Z6J2
- PHCI-2DPAGE:   Q9Z6J2
- GeneID:   1467790
- GeneID:   894864
- GeneID:   919831
- GeneID:   963128
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0783
- KEGG:   cpn:CPn1067
- KEGG:   cpt:CpB1111
- TIGR:   CP_0783
- HOGENOM:   HBG665227
- OMA:   MILPINI
- ProtClustDB:   PRK00150
- BioCyc:   CPNE115711:CP_0783-MONOMER
- BioCyc:   CPNE115713:CPN1067-MONOMER
- BioCyc:   CPNE138677:CPJ1067-MONOMER
- BioCyc:   CPNE182082:CPB1111-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 21018; Mature: 21018

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: ACT_SITE 142-142

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRLEYYGSPILRKKSSPIAEITDEIRNLVSDMCDTMEAHRGVGLAAPQVGKNVSLFVM
CCCCCCHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
CVDRETEDGELIFSESPRVFINPVLSDPSETPIIGKEGCLSIPGLRGEVFRPQKITVTAM
EECCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEEE
DLNGKIFTEHLEGFTARIIMHETDHLNGVLYIDLMEEPKDPKKFKASLEKIKRRYNTHLS
ECCCEEHHHHHCCEEEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
KEELVS
HHHHCC
>Mature Secondary Structure
MIRRLEYYGSPILRKKSSPIAEITDEIRNLVSDMCDTMEAHRGVGLAAPQVGKNVSLFVM
CCCCCCHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
CVDRETEDGELIFSESPRVFINPVLSDPSETPIIGKEGCLSIPGLRGEVFRPQKITVTAM
EECCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEEE
DLNGKIFTEHLEGFTARIIMHETDHLNGVLYIDLMEEPKDPKKFKASLEKIKRRYNTHLS
ECCCEEHHHHHCCEEEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
KEELVS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362