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The map label for this gene is ispH

Identifier: 15836548

GI number: 15836548

Start: 1165412

End: 1166344

Strand: Direct

Name: ispH

Synonym: CPj1017

Alternate gene names: 15836548

Gene position: 1165412-1166344 (Clockwise)

Preceding gene: 15836547

Following gene: 15836549

Centisome position: 95.01

GC content: 41.48

Gene sequence:

>933_bases
ATGAGAAAACTTATTTTATGCAATCCTAGAGGATTTTGCTCTGGAGTTGTGCGCGCTATTCAAGTTGTAGAGGTTGCTTT
AGAAAAGTGGGGAGCTCCTATCTATGTAAAACATGAGATTGTTCACAATCGCCATGTTGTTAATGCTTTACGAGCCAAGG
GAGCGATCTTTGTTGAAGAACTTGTTGATGTTCCTGAAGGTGAGAGAGTCATTTATTCAGCTCATGGAATTCCTCCTTCA
GTTAGAGCTGAAGCAAAAGCCCGTAAGCTTATTGATATTGATGCTACCTGTGGTTTGGTTACTAAGGTGCATTCTGCTGC
GAAGTTATACGCAAGTAAAGGATACAAAATCATACTGATCGGCCATAAGAAGCACGTTGAGGTGATTGGTATTGTTGGAG
AAGTTCCTGAACACATTACTGTTGTCGAGAAGGTTGCTGACGTCGAGGCCTTACCTTTTAGTTCTGATACACCTTTATTT
TATATTACTCAAACGACGTTGAGTTTGGATGATGTTCAGGAGATCTCATCGGCTTTGCTAAAGCGATATCCCTCTATCAT
TACTCTGCCTAGTTCTTCGATTTGTTATGCAACCACGAACCGTCAAAAAGCATTGCGTTCTGTTTTATCTCGCGTGAATT
ACGTCTATGTGGTTGGAGATGTCAACAGCTCGAATTCCAATCGTCTTCGCGAAGTGGCTTTGAGAAGGGGAGTTCCCGCT
GATTTGATCAACAATCCCGAGGATATTGATACGAACATCGTAAATCATTCTGGAGATATAGCAATGACTGCAGGAGCCTC
AACTCCCGAAGACGTAGTTCAAGCTTGCATTCGAAAGCTATCATCACTTATCCCTGGTTTACAAGTGGAAAATGATATAT
TTGCTGTAGAGGATGTCGTATTTCAATTACCAAAAGAACTCCGTTGTTCTTAG

Upstream 100 bases:

>100_bases
GACGTAATAGAATAATTTTTATTATTGCTTTAATATGCGCGCTTCCAATATAAGCATTGTGAAGCGCGTTTCATATGTCT
TTTATCTTTAGGTAATTATT

Downstream 100 bases:

>100_bases
GTCTTTAGGCTTACTTGCCAAGTTTTTCTCGAGATTGCTTTATAGAGTCTTCTTCTCGTTCAGAGAGGGTATTTACCTTT
TTAGTTCTCTGTATTTGAAA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MRKLILCNPRGFCSGVVRAIQVVEVALEKWGAPIYVKHEIVHNRHVVNALRAKGAIFVEELVDVPEGERVIYSAHGIPPS
VRAEAKARKLIDIDATCGLVTKVHSAAKLYASKGYKIILIGHKKHVEVIGIVGEVPEHITVVEKVADVEALPFSSDTPLF
YITQTTLSLDDVQEISSALLKRYPSIITLPSSSICYATTNRQKALRSVLSRVNYVYVVGDVNSSNSNRLREVALRRGVPA
DLINNPEDIDTNIVNHSGDIAMTAGASTPEDVVQACIRKLSSLIPGLQVENDIFAVEDVVFQLPKELRCS

Sequences:

>Translated_310_residues
MRKLILCNPRGFCSGVVRAIQVVEVALEKWGAPIYVKHEIVHNRHVVNALRAKGAIFVEELVDVPEGERVIYSAHGIPPS
VRAEAKARKLIDIDATCGLVTKVHSAAKLYASKGYKIILIGHKKHVEVIGIVGEVPEHITVVEKVADVEALPFSSDTPLF
YITQTTLSLDDVQEISSALLKRYPSIITLPSSSICYATTNRQKALRSVLSRVNYVYVVGDVNSSNSNRLREVALRRGVPA
DLINNPEDIDTNIVNHSGDIAMTAGASTPEDVVQACIRKLSSLIPGLQVENDIFAVEDVVFQLPKELRCS
>Mature_310_residues
MRKLILCNPRGFCSGVVRAIQVVEVALEKWGAPIYVKHEIVHNRHVVNALRAKGAIFVEELVDVPEGERVIYSAHGIPPS
VRAEAKARKLIDIDATCGLVTKVHSAAKLYASKGYKIILIGHKKHVEVIGIVGEVPEHITVVEKVADVEALPFSSDTPLF
YITQTTLSLDDVQEISSALLKRYPSIITLPSSSICYATTNRQKALRSVLSRVNYVYVVGDVNSSNSNRLREVALRRGVPA
DLINNPEDIDTNIVNHSGDIAMTAGASTPEDVVQACIRKLSSLIPGLQVENDIFAVEDVVFQLPKELRCS

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=47.5728155339806, Blast_Score=271, Evalue=3e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_CHLPN (Q9Z6P2)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   F86617
- PIR:   G72006
- RefSeq:   NP_225211.1
- RefSeq:   NP_301072.1
- RefSeq:   NP_445375.1
- RefSeq:   NP_877327.1
- GeneID:   1467734
- GeneID:   895489
- GeneID:   919793
- GeneID:   963021
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0836
- KEGG:   cpn:CPn1017
- KEGG:   cpt:CpB1055
- TIGR:   CP_0836
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- PhylomeDB:   Q9Z6P2
- ProtClustDB:   PRK01045
- BioCyc:   CPNE115711:CP_0836-MONOMER
- BioCyc:   CPNE115713:CPN1017-MONOMER
- BioCyc:   CPNE138677:CPJ1017-MONOMER
- BioCyc:   CPNE182082:CPB1055-MONOMER
- BRENDA:   1.17.1.2
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 33936; Mature: 33936

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLILCNPRGFCSGVVRAIQVVEVALEKWGAPIYVKHEIVHNRHVVNALRAKGAIFVEE
CCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHH
LVDVPEGERVIYSAHGIPPSVRAEAKARKLIDIDATCGLVTKVHSAAKLYASKGYKIILI
HHCCCCCCEEEEEECCCCCCHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCEEEEE
GHKKHVEVIGIVGEVPEHITVVEKVADVEALPFSSDTPLFYITQTTLSLDDVQEISSALL
ECCCCEEEEEEHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHH
KRYPSIITLPSSSICYATTNRQKALRSVLSRVNYVYVVGDVNSSNSNRLREVALRRGVPA
HHCCCEEEECCCCEEEEECCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCH
DLINNPEDIDTNIVNHSGDIAMTAGASTPEDVVQACIRKLSSLIPGLQVENDIFAVEDVV
HHCCCCCCCCCCEECCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHH
FQLPKELRCS
HHCCHHHCCC
>Mature Secondary Structure
MRKLILCNPRGFCSGVVRAIQVVEVALEKWGAPIYVKHEIVHNRHVVNALRAKGAIFVEE
CCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHH
LVDVPEGERVIYSAHGIPPSVRAEAKARKLIDIDATCGLVTKVHSAAKLYASKGYKIILI
HHCCCCCCEEEEEECCCCCCHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCEEEEE
GHKKHVEVIGIVGEVPEHITVVEKVADVEALPFSSDTPLFYITQTTLSLDDVQEISSALL
ECCCCEEEEEEHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHH
KRYPSIITLPSSSICYATTNRQKALRSVLSRVNYVYVVGDVNSSNSNRLREVALRRGVPA
HHCCCEEEECCCCEEEEECCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCH
DLINNPEDIDTNIVNHSGDIAMTAGASTPEDVVQACIRKLSSLIPGLQVENDIFAVEDVV
HHCCCCCCCCCCEECCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHH
FQLPKELRCS
HHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362